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Sterility


Normal Goat Serum Invitrogen™

Normal Goat Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Normal Horse Serum Invitrogen™

Normal Horse Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Cryopreserved Human Kupffer Cells Gibco™

Cryopreserved Human Kupffer Cells are isolated from adult human livers. Each vial contains a minimum of one million viable cells. These easy-to-use cells provide a convenient way to produce hepatocyte and Kupffer cell co-cultures for the study of various hepatic functions.

• Viability routinely >90%
• Purity routinely >90%
• Respond to activation with lipopolysaccharides (LPS)
• Minimum 1 million viable cells per vial
• Protocols for hepatocyte co-culture available

Kupffer Cells Provide for Improved Physiological Modeling
Growing evidence shows that under both normal and pathological conditions, many hepatocyte functions are regulated by substances released from neighboring non-parenchymal cells (NPC). These cells, particularly Kupffer cells, play an important role in the modulation of xenobiotic metabolism in the liver. Kupffer cells secrete potent mediators of the inflammatory response that controls liver inflammation. These cytokine mediators control hepatocyte metabolic rates through direct interactions with phase I and phase II enzymes. Kupffer cell and hepatocyte co-cultures can self-assemble within 72 hours of treatment with pro-inflammatory cytokines or LPS, and can function by effectively modulating P450 expression, thus giving a more physiologically relevant result. Studies indicate that co-culture of hepatocytes with NPCs better represent both normal liver physiology as well as disease states.

Normal Human Serum Invitrogen™

Normal Human Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Fetal Bovine Serum, qualified, Australia Gibco™

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Sera Category: Premium (Secure)
Origin: Australia.
Endotoxin level: <=5 EU/mL.
Hemoglobin level: <=30 mg/dL (levels routinely <=25 mg/dL).

Fetal Bovine Serum, mesenchymal stem cell-qualified, One Shot™ format, USDA-approved regions Gibco™

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Sera Category: Specialty
Specially tested for the ability to support the expansion and clonal enumeration (colony forming unit fibroblast assay) of mesenchymal stem cells.

Origin: Countries meeting USDA importation requirements.

Endotoxin level: <=50 EU/ml (levels routinely <=10 EU/ml).

Hemoglobin level: <=25 mg/dl.

Storage:
Store at -5°C to -20°C.

Mock (G) SLC Transporter Cells Gibco™

These cells are intended for use as a negative control in experiments utilizing TRANSiPORT solute carrier (SLC) transporter-overexpressing HEK293 cells. These mock cells do not overexpress the SLC transport protein and so can be used to determine levels of endogenous transport activity as well as non-specific binding of compounds being tested.

Related products
GM1102G Human OATP1B1 (G) SLC Transporter Cells
GM1103G Human OAT1 (G) SLC Transporter Cells
GM1104G Human OAT3 (G) SLC Transporter Cells
GM1105G Human OCT2 (G) SLC Transporter Cells
GM1106G Human OATP1B3 (G) SLC Transporter Cells
GM1108G Human OCT1 (G) SLC Transporter Cells
GM1113G Human NTCP (G) SLC Transporter Cells
GM1114G Human MATE1 (G) SLC Transporter Cells
GM1115G Human MATE2K (G) SLC Transporter Cells

Human Skeletal Myoblasts (HSkM) Gibco™

Primary Normal Human Skeletal Myoblasts (HSkM) are designed to undergo highly efficient differentiation following plating of cryopreserved cells.

The cells are:
• Performance tested—guaranteed to differentiate ≥ 50% following 48 hours of incubation
• Guaranteed to be ≥ 70% viable
• Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 viruses

Each vial contain sufficient number of cells to fully seed a single multi-well dish (ranging in format from 6-well to 384-well).

Monkey (cynomolgus) Cryopreserved Hepatocytes, Plateable Male Gibco™

These plateable cryopreserved male Cynomolgus Monkey hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, proper cell morphology, and metabolic activity increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• 75% ≤ viability
• Attachment efficiency
• Monolayer formation and integrity
• 80% ≤ confluency on Day 5
• Phase I and II enzyme activities

Related Links:

Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Fetal Bovine Serum, qualified, United States Gibco™

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Gibco Sera: Trusted Quality for Consistent Performance

Gibco Sera Category: Value Plus (Performance)
• Use for general cell culture with common cell lines
• Low endotoxin and hemoglobin levels
• Endotoxin level: < 10 EU/mL
• Hemoglobin level: < 25 mg/dL
• Origin: United States

Gibco Serum Delivers
• ISO 13485 certified, processed in FDA registered facilities
• Labeled for in-vitro diagnostics (compliant with highest level of USP sterility testing)
• Triple filtered at 0.1 µm
• Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
• Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

GeneBLAzer™ ERRα-UAS-bla HEK 293T Cells Thermo Scientific™

ERR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Estrogen related receptor alpha (ERR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ERR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ERR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of XCT790, an inverse agonist. In addition, ERR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

KM71H, Pichia pastoris Yeast Strain Invitrogen™

Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain.

Bovine Serum, New Zealand origin Gibco™

Gibco™ sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Sera Category: Secure
Origin: New Zealand.
Collected from cattle typically 12-24 months of age.

Human OATP1B1 (G) SLC Transporter Cells Gibco™

TRANSiPORT Human OATP1B1 (G) SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP1B1 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1B1 SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1101G) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1103G Human OAT1 (G) SLC Transporter Cells
GM1104G Human OAT3 (G) SLC Transporter Cells
GM1105G Human OCT2 (G) SLC Transporter Cells
GM1106G Human OATP1B3 (G) SLC Transporter Cells
GM1108G Human OCT1 (G) SLC Transporter Cells
GM1113G Human NTCP (G) SLC Transporter Cells
GM1114G Human MATE1 (G) SLC Transporter Cells
GM1115G Human MATE2K (G) SLC Transporter Cells
GM1101G Mock Cells

Horse Serum, New Zealand origin Gibco™

Gibco™ sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Sera Category: Specialty
Origin: New Zealand.
EIA tested (negative) serum from a donor herd.
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