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Cryopreserved Human Kupffer Cells Gibco™

Cryopreserved Human Kupffer Cells are isolated from adult human livers. Each vial contains a minimum of one million viable cells. These easy-to-use cells provide a convenient way to produce hepatocyte and Kupffer cell co-cultures for the study of various hepatic functions.

• Viability routinely >90%
• Purity routinely >90%
• Respond to activation with lipopolysaccharides (LPS)
• Minimum 1 million viable cells per vial
• Protocols for hepatocyte co-culture available

Kupffer Cells Provide for Improved Physiological Modeling
Growing evidence shows that under both normal and pathological conditions, many hepatocyte functions are regulated by substances released from neighboring non-parenchymal cells (NPC). These cells, particularly Kupffer cells, play an important role in the modulation of xenobiotic metabolism in the liver. Kupffer cells secrete potent mediators of the inflammatory response that controls liver inflammation. These cytokine mediators control hepatocyte metabolic rates through direct interactions with phase I and phase II enzymes. Kupffer cell and hepatocyte co-cultures can self-assemble within 72 hours of treatment with pro-inflammatory cytokines or LPS, and can function by effectively modulating P450 expression, thus giving a more physiologically relevant result. Studies indicate that co-culture of hepatocytes with NPCs better represent both normal liver physiology as well as disease states.

Human Umbilical Vein Endothelial Cells, HUVEC Pooled, (1 x 106 cells) Gibco™

Primary human umbilical vein endothelial cells, pooled from multiple donors, cryopreserved at the end of the primary culture.
• Viability at least 70%
• ≥1,000,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Positive for von Willebrand factor (vWf)
• Positive for CD31
• Negative for α-actin
• Positive for diI-Ac-LDL uptake

Human Epidermal Keratinocytes, Pooled (HEKp) Gibco™

Human Epidermal Keratinocytes, pooled (HEKp) from Invitrogen are normal cells isolated from multiple neonatal foreskins. These cells are optimal for investigating the molecular and biochemical bases of normal and disease processes of the skin. They have been cryopreserved at the end of the primary culture stage in animal origin-free media containing 10% DMSO. Use HEKp for your research into fibrosis, wound healing, irritancy, and diseases such as psoriasis.
Guaranteed to have ≥70% viability upon thawing
• Each vial guaranteed to reach ≥15 population doublings after thaw
• Sufficient cells to seed ≥100 cm2
• Tested for potential biological contaminants
• Optimized for use in EpiLife® serum-free media

Get More Physiologically Relevant Results with Invitrogen Primary Cells

Human primary cell cultures closely mimic the in vivo state and thus generate more physiologically relevant data. Our Human Epidermal Keratinocytes are isolated and pooled from 4-6 neonatal foreskins. This process is robust and yields large donor lots to ensure availability of consistent product for your studies.

Reliable Performance and Purity
Primary cells from Invitrogen are guaranteed to have ≥70% viability upon thawing with each vial containing sufficient cells to seed ≥100 cm2 of surface area. Performance testing guarantees each vial of HEKp will reach ≥15 population doublings after thaw. In addition, each lot is tested for potential biological contaminants--HIV-1, Hepatitis B, Hepatitis C viruses, mycoplasma, bacteria, yeast, and other fungi-- to ensure quality and robustness.

Optimized for Use with Gibco® Media
Human Epidermal Keratinocytes, pooled, from Invitrogen have been optimized for use with our serum-free media, specifically EpiLife® Medium with 60 µM calcium and Human Keratinocyte Growth Supplement (HKGS). Preparation of supplemented media is a simple process; the final solution can be stored for up to 30 days at 4°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Learn more about our complete system of media and supplements for culturing Human Epidermal Keratinocytes

Human Umbilical Vein Endothelial Cells, HUVEC Pooled, (0.5 x 106 cells) Gibco™

Primary human umbilical vein endothelial cells, pooled from multiple donors, cryopreserved at the end of the primary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Positive for von Willebrand factor (vWf)
• Positive for CD31
• Negative for α-actin
• Positive for diI-Ac-LDL uptake

Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Plateable Male Gibco™

These plateable cryopreserved male rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, proper cell morphology, and metabolic function increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Viability ≥ 75%
• Attachment efficiency
• Monolayer formation and integrity
• Confluency >80% on Day 5
• Phase I enzyme activities

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Coronary Artery Smooth Muscle Cells (HCASMC) Gibco™

Primary human coronary artery smooth muscle cells cryopreserved at the end of the tertiary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Negative for von Willebrand factor (vWf)
• Positive for a-actin

Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Plateable Female Gibco™

These plateable cryopreserved female rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

GeneBLAzer™ GR-UAS-bla HEK 293T Cells Thermo Scientific™

GR-UAS-bla HEK 293T cells contain the ligandbinding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-blaHEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-GR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. GR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of Dexamethasone. In addition, GR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Primary Mouse Cortical Neurons Gibco™

Gibco Primary Mouse Cortical Neurons are isolated and cryopreserved from C57BL/6 embryonic day-17 mice. A flexible, ready-to-use, and quality alternative to fresh neurons, each vial contains 1 million cells per mL. These neurons consistently yield post-thaw viability in the range of 50-80%, have high neuronal purity, and are validated for functionality.

• High viability: post-thaw viability of 50% or greater
• High purity: greater than 98% neuronal phenotype
• Functionally validated: tested for long-term survival, neurotransmitter response, and transduction

Dog (Beagle) Cryopreserved Hepatocytes, Male Gibco™

These cryopreserved male dog (Beagle) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Expi293F™ Cells Gibco™

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium. Expi293F cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.

Expi293F Cells attributes include:
Suspension culture cells pre-adapted to Expi293 Expression Medium
Demonstrated higher protein expression levels than other 293 cells adapted to Expi293 Expression Medium
Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells
Consistent transient expression performance of Expi293F cells for over 40 passages beyond thaw
• Also available from a cGMP bank (Cat. No. 100044202)

Frozen cells are supplied in a vial containing 1 ml of cells at 1 x 107 viable cells/ml in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.

Cryopreserved Rat Kupffer Cells

These cryopreserved purified Kupffer cells are isolated from adult male Sprague-Dawley rats. Each vial contains a minimum of one million cells.

Easy to use cryopreserved Kupffer cells provide a convenient way to produce custom cell ratios for hepatic co-culture.

Each lot of Cryopreserved Rat Kupffer Cells is tested for:

• Purity
• Response to activation with LPS
• Adherence
• Minimum 1 million cells per vial

Kupffer Cells for Improved Physiological Modeling

Growing evidence shows that under both normal and pathological conditions, many hepatocyte functions are regulated by substances released from neighboring non-parenchymal cells (NPC). These cells, particularly Kupffer cells, play an important role in the modulation of xenobiotic metabolism in the liver. Kupffer cells secrete potent mediators of the inflammatory response that controls liver inflammation. These cytokine mediators control hepatocyte metabolic rates through direct interactions with phase I and phase II enzymes. Kupffer cell and hepatocyte co-cultures can self-assemble within 72 hours of treatment with pro-inflammatory cytokines or LPS, and can function by effectively modulating P450 expression, thus giving a more physiologically relevant result. Studies indicate that co-culture of hepatocytes with NPCs would better represent both normal liver physiology as well as disease states.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

PichiaPink™ Expression Strain Set Invitrogen™

The PichiaPink™ Expression Strain Set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Expression System Kits. Each strain comes as a frozen stock in YPD media with 15% glycerol. All PichiaPink™ strains have the ade2 deletion. 3 strains have deletions of two protease genes, PEP4 and PRB1. You can use the PichiaPink™ Expression Strain Set to refill your PichiaPink™ Yeast Expression System Kits.

The PichiaPink™ Expression Strain Set includes the following Pichia strains:
•     PichiaPink™ Strain 1: ade2
•     PichiaPink™ Strain 2: ade2, pep4
•     PichiaPink™ Strain 3: ade2, prb1
•     PichiaPink™ Strain 4: ade2, prb1, pep4

Select Transformants by Color
The PichiaPink™ Yeast Expression System makes it easy to select for transformed colonies by color. Untransformed PichiaPink™ strains form pink colonies. When grown on plates without adenine, transformed PichiaPink™ strains form white colonies (Fig 1).

Get Reduced Protein Degradation
The PichiaPink™ Expression Strain Set comes with 3 strains that include knockouts in two protease genes. Protease-deficient strains reduce the need for protease inhibitors when growing your cells and improve the yield of your protein. Test a single protease knockout or the double protease knockout to find the one that works best for you.

Why Choose the PichiaPink™ Yeast Expression System?
The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.

For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links
Learn more about the PichiaPink™ Yeast Expression System.
Learn more about our other protein expression systems.

References

1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]
2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]

Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Male Gibco™

These cryopreserved male rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Rat (Wistar) Cryopreserved Hepatocytes, Plateable Male Gibco™

These plateable cryopreserved male rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
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