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Primary Rat Cortex Neurons Gibco™

Gibco Primary Rat Cortex Neurons are isolated and cryopreserved from day-18 rat embryos. A flexible, ready-to-use and quality alternative to fresh neurons, each vial contains 1 million cells per mL. These neurons consistently yield post-thaw viability in the range of 50–80% and have high neuronal purity.

ExpiSf9™ Cells Gibco™

ExpiSf9 Cells are a non-engineered derivative of Sf9 insect cells that have been adapted for high-density suspension growth in ExpiSf CD Medium.

ExpiSf9 Cells:
• Enable consistent protein expression performance for over 20 passages after thaw
• Allow you to attain yields of three times more protein per liter than other insect cells from baculovirus-infected culture as part of the ExpiSf Expression System
• Achieve maximum cell densities of 2x107 cells per mL in ExpiSf CD Medium
• Exhibit fast recovery post-thaw and doubling times of approximately 24 hours
• Achieve high performance growth in multiple culture volumes from 4 mL in deep-well plates to >3 L in shake flasks

ExpiSf9 Cells are a core component of the ExpiSf Expression System. Frozen cells are supplied in a vial containing 1.5 mL of cells at 1 x 107 viable cells per mL in ExpiSf CD Medium and 7.5% DMSO. The cells should be thawed in ExpiSf CD Medium. Cells are typically grown in shake flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator. Growing ExpiSf9 cells in adherent format for routine culture is not recommended.

QuantiGene™ Sample Processing Kit, cultured cells Invitrogen™

This QuantiGene Sample Processing Kit for cultured cells contains reagents and instructions for the preparation of cell lysates for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA targets and QuantiGene Plex DNA assays for DNA targets.

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your cultured cell sample

What you get
The QuantiGene Sample Processing Kit for cultured cells contains our proprietary lysis mixture, proteinase K, and detailed instructions for preparing cell lysates from cultured cells.

Success in cell lysis
Key factors that influence the complete lysis of the cells include:
• The lysis mixture
• Proteinase K
• Vortexing or pipetting of the sample
• Storage of the lysates at -80°C
• Incubation at 50°C

The protocols provided with the QuantiGene Sample Processing Kit for cultured cells use a combination of these factors to ensure complete lysis of the samples. In addition, the lysis mixture provides the proper ionic conditions for the overnight hybridization in the QuantiGene assays.

Human Spheroid-Qualified Hepatocytes Gibco™

Gibco Human Spheroid-Qualified Hepatocytes are off-the-shelf cryopreserved human cells for generation of hepatic spheroids in your own lab. Each vial contains a minimum of five million viable cells, sufficient for up to sixteen 96-well plates of hepatic spheroids.

Maintain cells in culture longer—up to 21 days, >4X longer than cells last in monolayer culture
Design your own schedule—cells are ready for plating when you are
Interrogate pathways of interest—applicable for toxicity, metabolism, or disease modeling

Simplified protocol
Cryopreserved cells can be thawed and plated at your convenience, in as little as 60 minutes. Try it on your own, or request a demo with an experienced scientist.

Stringent quality control
Our high standards help ensure only the best spheroid-forming lots become available, maximizing your chance of success. In addition, each lot is tested for:
• Albumin production in monolayer and spheroid culture
• Phase I enzyme induction
• Bile duct formation (CFDA staining)
• Proper morphology
• Viability >75%
• Attachment efficiency
• Monolayer formation and integrity

Important! For best results, do not attempt hepatic spheroid formation in low attachment plates from other sources. Hepatocyte lots are qualified in Nunclon Sphera plates only and use of other plates can lead to variability in spheroid formation.

Flexible readouts
Analyze single spheroids or pool several for detection in assays such as gene expression, immunostaining, viability, or CYP activity.

Primary Rat Hippocampus Neurons Gibco™

Gibco Primary Rat Hippocampus Neurons are isolated and cryopreserved from day-18 rat embryos. A flexible, ready-to-use and quality alternative to fresh neurons, each vial contains 1 million cells per mL. These neurons consistently yield post-thaw viability in the range of 50–80% and have high neuronal purity.

GeneBLAzer™ GR-UAS-bla HEK 293T Cells Thermo Scientific™

GR-UAS-bla HEK 293T cells contain the ligandbinding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-blaHEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-GR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. GR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of Dexamethasone. In addition, GR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Mouse (CD-1) Cryopreserved Hepatocytes, Male Gibco™

These cryopreserved male mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, proper cell morphology, and metabolic function increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Viability ≥ 70%
• Proper morphology
• Phase I and II enzyme activities

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

KM71H, Pichia pastoris Yeast Strain Invitrogen™

Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain.

Human Plateable Hepatocytes, Transporter Qualified Gibco™

Plateable cryopreserved human hepatocytes assessed for transporter function using uptake transporter assays at both 4°C and 37°C.

Cells per vial: 4-8 million

Hepatocyte cultures were incubated with taurocholate and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP/BSEP and OATPs/MRP2. Accumulation rates and facilitated active uptake were calculated.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, proper cell morphology, and transporter function increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Viability ≥ 70%
• Attachment efficiency
• Monolayer formation and integrity
• Confluency >80% on Day 4
• Tested for plating in 96 well format
• Phase I enzyme activities
• Transporter activity
• Bile canaliculi formation
• Relevant CYP450 polymorphisms

Related Links:

Learn more about our cryopreserved hepatocytes
Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

GeneBLAzer™ LXRβ-UAS-bla HEK 293T Cells Thermo Scientific™

LXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-beta (LXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-LXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. LXR beta-UAS-bla HEK 293T 293 cells are functionally validated for Z' and EC50 concentrations of TO901317. In addition, cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Expi293F™ Cells Gibco™

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium. Expi293F cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.

Expi293F Cells attributes include:
Suspension culture cells pre-adapted to Expi293 Expression Medium
Demonstrated higher protein expression levels than other 293 cells adapted to Expi293 Expression Medium
Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells
Consistent transient expression performance of Expi293F cells for over 40 passages beyond thaw
• Also available from a cGMP bank (Cat. No. 100044202)

Frozen cells are supplied in a vial containing 1 ml of cells at 1 x 107 viable cells/ml in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.

GeneBLAzer™ ERα-UAS-bla GripTite™ Cells Thermo Scientific™

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla GripTite™ cell line. GeneBLAzer®UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Cryopreserved Rat Kupffer Cells

These cryopreserved purified Kupffer cells are isolated from adult male Sprague-Dawley rats. Each vial contains a minimum of one million cells.

Easy to use cryopreserved Kupffer cells provide a convenient way to produce custom cell ratios for hepatic co-culture.

Each lot of Cryopreserved Rat Kupffer Cells is tested for:

• Purity
• Response to activation with LPS
• Adherence
• Minimum 1 million cells per vial

Kupffer Cells for Improved Physiological Modeling

Growing evidence shows that under both normal and pathological conditions, many hepatocyte functions are regulated by substances released from neighboring non-parenchymal cells (NPC). These cells, particularly Kupffer cells, play an important role in the modulation of xenobiotic metabolism in the liver. Kupffer cells secrete potent mediators of the inflammatory response that controls liver inflammation. These cytokine mediators control hepatocyte metabolic rates through direct interactions with phase I and phase II enzymes. Kupffer cell and hepatocyte co-cultures can self-assemble within 72 hours of treatment with pro-inflammatory cytokines or LPS, and can function by effectively modulating P450 expression, thus giving a more physiologically relevant result. Studies indicate that co-culture of hepatocytes with NPCs would better represent both normal liver physiology as well as disease states.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

GeneBLAzer™ RARβ-UAS-bla HEK 293T Cells Thermo Scientific™

RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

Mouse (CD-1) Cryopreserved Hepatocytes, Female Gibco™

These cryopreserved female mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.
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