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    MicroSEQ™ ID Microbial Identification Software v3.0 Bundle for 3500 Instrument Applied Biosystems™

    MicroSEQ® ID Microbial Identification Software allows you to easily identify and classify unidentified bacterial or fungal sequences by comparing them to a validated microbial library. This bundled version includes the bacterial and fungal sequence libraries.

    A validated library of over 2300 microbial 16S rDNA sequences help ensure you get the answers you need

    • Automatically identify unknown specimens
    • Run plates on Applied Biosystems® 3500 Series genetic analyzers directly via the MicroSEQ® ID Software
    • MicroSEQ® system is a complete integrated solution, including security, audit, and e-signature features
    • Qualification and validation support

    Confidently Identify Microbes
    The MicroSEQ® system comprises bacterial libraries of 500 bp 16S rDNA sequences and over 2300 validated entries--including extensive coverage of gram-negative nonfermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. A fungal library containing more than 1100 entries is also included for fungal identification. To help ensure accurate identification of the species of your sequences, we rigorously validate all library entries. And unlike other widely available databases, MicroSEQ® libraries contain only correctly identified organisms and quality-controlled sequences derived from type strains of preeminent culture collections. The libraries also support sequence polymorphisms, and include bacterial Category A biothreat agents.

    Automatically Identify Unknown Specimens
    Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The new Auto-ID feature uses a set of editable parameters to automatically identify unknown specimens. The results table is displayed in the report, and an editable comments field allows additional information or manual identifications to be entered into the same table.

    Run Plates on 3500/3500xL Instruments Directly via the MicroSEQ® ID Software
    Applied Biosystems® 3500 Series Genetic Analyzer users are now able to run plates on these instruments directly via the MicroSEQ® ID Software user interface, without needing Autoanalysis Manager. A single software wizard enables you to set up projects, specimens, and plates; adjust the plate layout via drag and drop; start and monitor the run; and review results.

    The MicroSEQ® System: A Complete, Integrated Solution
    Streamlining every step in microbial identification, the Applied Biosystems® MicroSEQ® system combines the advantages of MicroSEQ® ID Software with easy to use PrepMan® Ultra sample preparation reagents and protocols, MicroSEQ® bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. The MicroSEQ® ID Software also includes security, audit, and electronic signature features enabling the software to smoothly integrate into your workflow.

    Qualification and Validation Support
    To help ensure seamless integration into your laboratory, from installation to routine operation, we offer comprehensive installation and operational qualification (IQ/OQ) and validation support services.

    DS-33 GeneScan™ Install Kit Dx with GeneScan™ 600 LIZ™ Size Standard v2.0 Dx Applied Biosystems™

    The DS-33 GeneScan™ Installation Kit Dx consists of pooled PCR products labeled with 6-FAM™, VIC™, NED™, and PET™ dyes. To generate the pooled products, CEPH individual 1347-02 genomic DNA is amplified with 8 fluorescent PCR primer pairs, which amplify selected microsatellite loci (as listed in the package product insert). The kit also contains a tube of GeneScan 600 LIZ™ Size Standard v2.0 Dx (Cat. No. A25794).

    Ion Xpress™ Plus Fragment Library Kit Ion Torrent™

    The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100 ng input DNA. In addition, highly efficient library construction enables the creation of 'amplification-free' libraries from 1 µg input material. This kit allows users to modulate an appropriate fragment insert size depending on desired read length and application.

    The Ion Xpress™ Plus Fragment Library Kit contains sample preparation reagents for enzymatic shearing and library construction for up to 20 DNA libraries (depending on input DNA type) for semiconductor sequencing. Store components at -20°C.

    The Ion Xpress™ Plus Fragment Library Kit offers you:
    • Superior coverage uniformity for diverse sample types using our proprietary Ion Shear™ Plus chemistry
    • Lower input requirement with as little as 100 ng starting material
    • Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes in as fast as 2 hours
    • Significant cost savings by removing the need for ancillary shearing devices
    • Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

    A Single-Day Workflow: The Next Stage in a Sequencing Revolution
    With a turnaround time of as little as 2 hours, the Ion Xpress™ Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your sample(s) of interest possible in less time than ever before. Furthermore, Ion Xpress™ Plus fragment libraries exhibit greater coverage uniformity and higher recovery rates across diverse sample types than other library construction methodologies, so you can be confident of generating the best data in the shortest time.

    Ion Torrent Sequencing Made Simpler, Faster, and More Affordable
    Ion Shear™ Plus DNA fragmentation technology, included in the Ion Xpress™ Plus kit, obviates the need for costly physical shearing devices for most applications and requires as little as 100 ng of sample, accelerating your path to semiconductor sequencing. This flexible technology provides users the ability to adjust the resulting DNA fragment size to accommodate a range of sequencing read lengths depending on their project requirements. This technology is also amenable to automated library preparation systems such as the AB Library Builder™ System to provide quick answers when you need them most. Ion Shear™ Plus DNA fragmentation technology is not included in the Ion Plus kit. Use the Ion Plus kit if physical shearing methods are preferred.

    For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

    CPG (Controlled Pore Glass) Synthesis Column, 1.0 µmol, 500 Angstrom Pore Size, dG(dmf) Applied Biosystems™

    The 1.0 µmol dG(dmf) Controlled Pore Glass (CPG) columns are suitable for the synthesis of oligonucleotides<50 bases in length. These columns deliver high-quality oligonucleotides.

    • Proprietary design and manufacturing methods ensure consistency and high quality
    • Each column is tested to verify accuracy and precision of fill, then pressure-tested to verify proper assembly and to guarantee each column is leak-free
    • The 500 angstrom pore size allows synthesis of oligonucleotides of up to 50 bases.

    These columns are fully compatible with the Applied Biosystems 3400 instruments, ABI 391, ABI 392, ABI 394, and earlier instruments.

    For Research Use Only. Not for use in diagnostics procedures.

    Trap-Pak™ Bags (Mini) Applied Biosystems™

    Trap-Pak™ bags are tightly sealed membrane packets containing molecular sieves to reduce and maintain a low water content in solutions that must remain anhydrous.

    Used With:
    Anhydrous Acetonitrile, Amidite Diluent, Tetrazole⁄Acetonitrile, Amidite Activator Solution, Tetrazole⁄Acetonitrile, Amidite Activator Solution, Tetrazole⁄Acetonitrile, Amidite Activator Solution

    For Research Use Only. Not for use in diagnostics procedures.

    AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit & Prep-n- Go™ Buffer, for buccal swabs Applied Biosystems™

    The AmpFLSTR Identifiler Direct PCR Amplification Kit & Prep-n-Go Buffer (for Buccal Swabs) combines the features of our AmpFLSTR Identifiler Direct PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for Buccal Swabs). The Identifiler Direct kit enables direct PCR amplification of the same 16 loci included in our standard Identifiler kit, while eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Prep-n-Go Buffer (for Buccal Swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

    Key Features:

    • Enables the direct amplification of buccal swab samples without the need for extraction or purification
    • Facilitates high throughput & automated sample processing
    • Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
    • Helps accelerate time to result with minimal reduction in data quality
    • Minimizes extraction?purification costs and helps reduce the cost of labor and consumables

    In addition, the Identifiler Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Identifiler kit, which:

    • Maximizes concordance with STR profiles generated with other AmpFLSTR kits including the Identifiler, Identifiler Plus, Profiler Plus, Profiler Plus ID, SGM Plus and COfiler Kits
    • Facilitates comparison with casework data and legacy databases
    • Streamlines validation and implementation

    For Forensic or Paternity Use Only.

    Important Notice

    The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

    SOLiD™ EZ Bead™ E80 System Consumables, for TargetSeq™-enriched samples Applied Biosystems™

    SOLiD™ EZ Bead™ E80 System Consumables for TargetSeq™, 4-pack, contains all necessary reagents and consumables for performing four E80 EZ Bead™ system runs for TargetSeq™-enriched samples, except the SOLiD™ EZ Bead™ Oil Pack Kit, which can be purchased separately.

    Included in the kit:

    4 SOLiD™ EZ Bead™ Emulsifier E80 Reagents Kits
    4 SOLiD™ EZ Bead™ Emulsifier E80 Accessories Kits
    2 SOLiD™ EZ Bead™ Amplifier E80 Accessories Kits
    4 SOLiD™ EZ Bead™ Enricher Buffer Reagents Kits
    4 SOLiD™ EZ Bead™ Enricher E80 Reagents Kits
    4 SOLiD™ EZ Bead™ Enricher Accessories Kits

    For Research Use Only. Not for human or animal therapeutic or diagnostic use.

    Genexus™ Barcodes 1-32 AS Ion Torrent™

    The Ion Torrent Genexus Barcodes 1-32 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 33-64 AS, 65-96 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

    As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-32 AS kit:
    • Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
    • Supports intake variability of clinical research samples in a cost-effective manner
    • Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
    • Enables parallel processing of up to four compatible assays in a single run
    • Delivers fast and simple instrument setup, with less than five minutes of hands-on time

    The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

    Plate Base (septa seal) for 3730/3730xl, 384 well Applied Biosystems™

    This is a 384-well Plate Base (Septa Seal) for use with Applied Biosystems® 3730 and 3730xl DNA Analyzers.

    For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

    Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems Invitrogen™

    The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

    The Collibri Stranded RNA Library Prep Kit offers:
    • Short, automation-friendly workflow. mRNA can be converted to sequencing-ready library within 4.5 hours.
    • Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
    • Uniform transcript coverage and high transcript detection sensitivity
    • High sensitivity of differential gene expression detectio
    • >98% strand specificity
    • Efficient detection of non-coding RNA

    The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~150 bp for mRNA or whole transcriptome libraries with sample inputs of 1–25 ng of mRNA-enriched or ribo-depleted RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based library purification steps, enable completion of the entire workflow in approximately 4.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

    For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

    Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The Collibri Stranded RNA Library Prep Kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

    A related product, the Collibri Library Quantification Kit is recommended for qPCR-based quantification of libraries before proceeding to sequencing.

    Applications
    The Collibri Stranded RNA Library Prep Kit is recommended for:
    • Gene expression studies
    • Alternative splicing analysis
    • Non-coding RNA detection and discovery
    • Gene fusion detection
    • Detection of transcript isoforms

    CPG (Controlled Pore Glass) Synthesis Column, 500 Angstrom, 10.0 µmol, dG(dmf) Applied Biosystems™

    The 10.0 µmol dG(dmf) Controlled Pore Glass (CPG) columns are suitable for the synthesis of oligonucleotides<50 bases in length. These columns deliver high-quality oligonucleotides.

    • Proprietary design and manufacturing methods ensure consistency and high quality
    • Each column is tested to verify accuracy and precision of fill, then pressure-tested to verify proper assembly and to guarantee each column is leak-free
    • The 500 angstrom pore size allows synthesis of oligonucleotides of up to 50 bases.

    These columns are fully compatible with the Applied Biosystems 3400 instruments, ABI 391, ABI 392, ABI 394, and earlier instruments.

    For Research Use Only. Not for use in diagnostics procedures.

    GeneArt™ Type IIs Assembly Kit, Aar I Invitrogen™

    The GeneArt® Type IIs Assembly Kit, Aar I, provides seamless cloning and assembly of up to 8 DNA fragments by simultaneous cleavage and ligation in a single reaction. The kit uses a technology similar to Golden Gate cloning with the type IIs restriction enzyme Aar I and can be used to assemble multiple fragments in a pre-determined order into any compatible vector. Since type IIs assembly is not based upon homologous recombination, there is minimal risk of rearrangements and minimal sequence confirmation of your final construct is required.

    The kit is offered in three versions, each with a different type IIs restriction enzyme (Aar I, Bsa I, or Bbs I). All versions include all-in-one enzyme mix, cloning vector, and cloning controls. Our GeneArt® Primer and Construct Design Tool should be used to determine the appropriate GeneArt® assembly kit for your fragments; it provides an easy-to-use interface to design your construct and create and order primers, should they be required.

    • Assemble multiple DNA fragments in any order, into any compatible vector, without scars
    • Avoid homologous recombination and associated rearrangements when cloning homologous or repetitive sequences
    • Use for assembly of TALs, gene variants, and repetitive sequences
    • Create your own cloning and expression vectors with custom vector elements
    • Minimize sequence confirmation of final construct
    • Pick from three type IIs enzymes

    Type IIs Cloning
    GeneArt® Type IIs Cloning is a simple, two-step process, consisting of an in vitro assembly reaction followed by transformation into competent E. coli. The type IIS restriction endonuclease Aar I recognizes an asymmetric DNA sequence and cleaves the DNA molecule at a defined distance from the Aar I recognition site. The ends of your DNA fragment can be designed to be flanked by the Aar I restriction site such that digestion of the fragments removes the recognition site and generates complementary overhangs that can be ligated seamlessly, creating a junction that lacks the original site. The kit includes a destination vector, called pType IIs, which is optimized for this approach.

    Cloning Efficiency, Flexibility, and Precision
    With the GeneArt® Type IIs Assembly Kit, the main factors effecting cloning efficiency are the size of the DNA elements, the total size of the final molecule (? 10 kb), and the quality and specificity of the fragment.

    Typical cloning efficiencies for different numbers of fragments cloned into pType IIs are:

    • >95% for 5 fragments of 1 kb each
    • >60% for 8 fragments of 1 kb each
    • >85% for 2 identical fragments of 1 kb each

    in silico Cloning Design Support
    A key step in GeneArt® Type IIs Cloning is the correct design of fragments and oligos with the appropriate recognition sites and spacing to help ensure successful assembly of your clone. To simplify and speed the design process we provide the GeneArt® Primer and Construct Design Tool to help you design your experiment in silico. The tool recommends the correct GeneArt® kit for your assembly, checks for compatibility of the experimental design with the product specifications, designs DNA oligos, if needed, and presents the user with a graphical representation of the vector, as well as a downloadable annotated sequence in GenBank format that is compatible with VectorNTI® software.

    Additional recommended products:

    • Zero Blunt® TOPO® PCR Cloning Kit, without competent cells (Cat. No. 450245)
    • Zero Blunt® TOPO® PCR Cloning Kit, with One Shot® TOP10 Chemically Competent E. coli (Cat. No. K280020)
    • One Shot® MAX Efficiency™ DH10B™ T1 Phage Resistant Cells (Cat. No. 12331-013)

    Yfiler™ Plus PCR Amplification Kit Applied Biosystems™

    Note: The Yfiler Plus kit is now approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

    The Yfiler Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.

    The Yfiler Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.

    Key features of the Yfiler Plus PCR Amplification Kit:
    • Improved discrimination capacity through new, highly discriminating markers
    • Improved performance with degraded samples and PCR inhibitors
    • Improved genotyping accuracy through expanded allelic ladder and virtual bins
    • Concordance with existing data in reference haplotype databases
    • Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits

    Improved discrimination capacity
    The Yfiler Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR Yfiler PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.

    More information from challenging casework samples
    Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.

    Streamlined protocol and increased workflow efficiency
    The Yfiler Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
    • Blood and buccal samples on treated paper substrates, without the need for sample purification
    • Blood samples collected on untreated paper substrates and treated with Prep-n-Go Buffer
    • Buccal samples collected on swab substrates and treated with Prep-n-Go Buffer

    Complete validated solution
    Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

    For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

    Oncomine™ Myeloid Research Assay GX Ion Torrent™

    The Ion Torrent Oncomine Myeloid Assay GX is a comprehensive targeted next-generation sequencing (NGS) assay designed for sensitive detection of myeloid disorder-associated DNA mutations and fusion transcripts in blood and bone marrow samples.

    This assay is compatible with the Genexus Integrated Sequencer, which performs library preparation, sequencing, analysis, and reporting in an automated sample-to-result workflow. Depending on the workflow, results can be obtained in as little as a single day.

    The Oncomine Myeloid Assay GX includes the following features:
    • Comprehensive coverage of key DNA mutations and fusion transcripts associated with myeloid disorders
    • Automated sample-to-report workflow on the Genexus sequencer in less than a day
    • Sequence up to eight samples (DNA & RNA) per lane on an Ion Torrent GX5 Chip in a single run
    • Less than 15 minutes hands-on time
    • Detection of somatic variants down to 5% allele frequency

    The Oncomine Myeloid Assay GX is designed to assist in the understanding of myeloid cancer. The DNA panel is comprised of 40 key genes while the RNA panel includes a broad fusion panel of 29 driver genes, covering the most relevant targets associated with major myeloid disorders, including acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).

    The multiplex primer design leverages Ion AmpliSeq technology to generate results from multiple samples in a single run. Sequencing results are automatically analyzed by Genexus software using an optimized assay-specific analysis workflow.

    The assay kit includes three pools of Ion AmpliSeq oligonucleotide primers (a 2-pool DNA panel and 1-pool RNA panel) and Genexus Strips 1 & 2-AS library reagents, enough to sequence up to 32 samples on the Genexus Integrated Sequencer. One end-to-end sequencing run uses one lane on the Ion Torrent GX5 Chip and accommodates up to eight samples. Additional Genexus sequencer reagents and supplies must be ordered separately.

    CPG (Controlled Pore Glass) Synthesis Column, 500 Angstrom, 10.0 µmol, dC(Bz) Applied Biosystems™

    The 10.0 µmol dC(Bz) Controlled Pore Glass (CPG) columns are suitable for the synthesis of oligonucleotides<50 bases in length. These columns deliver high-quality oligonucleotides.

    • Proprietary design and manufacturing methods ensure consistency and high quality
    • Each column is tested to verify accuracy and precision of fill, then pressure-tested to verify proper assembly and to guarantee each column is leak-free
    • The 500 angstrom pore size allows synthesis of oligonucleotides of up to 50 bases.

    These columns are fully compatible with the Applied Biosystems 3400 instruments, ABI 391, ABI 392, ABI 394, and earlier instruments.

    For Research Use Only. Not for use in diagnostics procedures.
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