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    Converge™ Software v1.0 Case Management License and Kinship & Paternity Analysis License Applied Biosystems™

    These two licenses are for the Case Management and Kinship & Paternity Analysis applications of Converge Software v1.0. The software is obtained through purchase of the Converge Software documentation and server computer. Converge Software is an all-in-one, modular, enterprise platform from Thermo Fisher Scientific that provides integrated DNA data management and analysis software for forensic laboratories. The software simplifies the daily complexities of forensic case management and kinship/paternity analysis and centralizes all case data under one powerful solution.

    Case Management application
    Converge Software is designed to increase the efficiency of forensic and relationship DNA testing laboratories through the centralization of data creation, analysis, and storage to a single access point. The Case Management software application supports case, subject, genotype profile, and laboratory data management, and allows for automated data transfer and integration with various forensic DNA laboratory systems. The software is highly configurable to fit specific laboratory workflows, not only for analysis parameters, but also for data fields per the laboratory SOPs. In addition, it allows for configuration of the user interface and output reports, and provides features that help ensure data security and integrity. These features are easily accessible through a secured web browser interface under the protection of a laboratory’s own IT department. A variety of software modules can be readily built on the Converge platform to support various forensic applications.

    Converge Kinship and Paternity application
    Kinship and paternity testing can provide challenges in both the sheer volume of cases to be analyzed, as well as the complexity of laboratory workflows and statistical analyses being performed. Thermo Fisher Scientific offers an integrated and streamlined solution for kinship and paternity testing on the Converge platform with its Converge Kinship and Paternity application. The application integrates with Converge Software and GeneMapper™ ID-X genotyping software to help enable automated analysis, reporting of routine paternity and other relationship testing cases, and allows for genetic likelihood ratio calculation, as well as reports for any complex relationship. Licenses for this module may be purchased in addition to the Case Management license.

    Learn more about Case Management License (without Kinship & Paternity Analysis License) >

    User Bulletin: 310 Genetic Analyzer Applied Biosystems™

    This is a bulletin describing the use of the G5v2 Module for Use with Dye Set 33 (DS-33) and 5-dye applications.

    For Research Use Only. Not for use in diagnostics procedures.

    DS-32 Matrix Standard Set [5FAM™, JOE™, NED™ and ROX™ dyes] for the 310 Genetic Analyzer Applied Biosystems™

    The Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing DNA fragments on the 310 Genetic Anlayzer. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the five different colored fluorescent dye-labeled samples in a single capillary.

    For Research Use Only. Not for use in diagnostics procedures.

    Septa for 0.2 mL MicroAmp™ tubes (for the 310 Genetic Analyzer) Applied Biosystems™

    These are septa covers for 0.2 ml MicroAmp™ sample tubes, for use with the 310 Genetic Analyzer.

    Use with: MicroAmp® 8-Tube Strips (0.2 ml).

    For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

    CE Phosphoramidite, dC(Bz) Applied Biosystems™

    The dC(Bz) CE Phosphoramidite is used for nucleic acid synthesis on ABI DNA Synthesizers. Standard series of phosphoramidite deprotects in 6-8 hours at 55°C.

    β-cyanoethyl phosphoramidites are for use on ABI synthesizers to guarantee you high quality nucleic acid synthesis.

    • Proprietary manufacturing processes yield high-purity β-cyanoethyl phosphoramidites, which in turn guarantee production of high-quality DNA at all scales.
    • β-cyanoethyl phosphoramidites are use-tested and optimized for use on the ABI synthesizers to guarantee you high-quality nucleic acid synthesis.

    High Quality
    Our phosphoramidites benefit from proprietary manufacturing processes that yield high-purity products. In synthesis they perform at a correspondingly high level, producing pure, high quality DNA at all scales. We guarantee that 5'-dimethoxytrityl nucleoside β-cyanoethyl phosphoramidites, monomer intermediates for licensed β-cyanoethyl phosphoramidite chemistry, will produce DNA that is at least 99.8% chemically authentic when used with recommended synthesis cycles.

    Dry phosphoroamidites (powder form) are guaranteed for one year from the date of shipment. Lifetime of diluted phosphoramidites in residence on the instrument is two weeks when used with CPG synthesis supports, and three weeks when used with polystyrene supports.

    For Research Use Only. Not for use in diagnostics procedures.

    Ion AmpliSeq™ Comprehensive Cancer Panel Ion Torrent™

    The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. View the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (PDF).

    The Ion AmpliSeq™ technology allows ultra-high multiplex PCR combined with targeted sequencing library construction in just 16 hours for over 400 genes. Utilizing only 10 ng of input DNA per primer pool for a total of 40 ng of input DNA, analysis of restricted samples like FFPE samples is possible. The Ion AmpliSeq™ Comprehensive Cancer Panel is optimized for library construction with the Ion AmpliSeq™ Library Kit 2.0. Libraries are then ready for template preparation on the Ion OneTouch™ System and sequencing on the Ion PGM™ Sequencer. The Ion AmpliSeq™ Comprehensive Cancer Panel is not compatible with Ion Ampliseq™ 2.0 Beta Kits.

    Features and Benefits:

    • Broad survey of 409 key genes in a simple PCR reaction, no additional capital equipment required
    • Unmatched plexy of 16,000 primer pairs in only four pools with Ion AmpliSeq™ technology
    • Low DNA input of only 40 ng DNA and short amplicons enable FFPE samples and needle biopsies
    • Start comprehensive genomic studies quickly with pre-designed primer pools
    • Simplify analysis and annotation of variants with Ion Reporter™ software

    Comprehensive Gene Content Including Genes from Hotspot Panel
    Ion Torrent has designed the most extensive cancer panel available for sequencing with Ion PGM™ and, soon, Ion Proton™ Sequencers. Working with key cancer researchers and reviewing literature and databases, the Ion AmpliSeq™ Comprehensive Cancer Panel was designed to target all exons of key tumor suppressor genes and oncogenes most frequently cited and most frequently mutated. Strategically designed to interrogate CDS and splice variants across multiple gene families simultaneously, pathway-based gene selection profiles mutational spectrum in cancer driver genes and drug targets, along with signaling cascades, apoptosis, DNA repair, transcription regulators, inflammatory response, and growth factor genes in a single assay. Additionally, all genes from the focused Ion AmpliSeq™ Cancer Panel are included in the Ion AmpliSeq™ Comprehensive Cancer Panel providing corresponding complementary data.

    Unmatched Level of Multiplex PCR
    The Ion AmpliSeq™ Comprehensive Cancer Panel is comprised of four primer pools totaling almost 16,000 primer pairs covering 409 genes-that's approximately 4,000 primer pairs in each pool! Used in conjunction with the Ion AmpliSeq™ Library Kit 2.0, the Ion AmpliSeq™ Comprehensive Cancer Panel maintains high uniformity and specificity at this high plexy, advancing multiplex PCR to new levels.

    Over 400 Genes, Only 40 ng DNA Required
    While other target selection methods typically require micrograms of DNA input, the Ion AmpliSeq™ Comprehensive Cancer Panel uses only 40 ng DNA. This breakthrough in low DNA input requirement enables the interrogation of restricted samples like formalin-fixed, paraffin-embedded samples (FFPE) and fine needle aspiration biopsies (FNAB). Additionally, shorter amplicon design (average 155 bp) allows for compatibility with degraded samples.

    Simplified Target Selection Without Primer Design
    With pre-designed primers in the Ion AmpliSeq™ Comprehensive Cancer Panel, researchers can bypass gene selection, primer design, and optimization, and advance straight to generating results for a broad survey of hundreds of genes in key research samples. To further focus your cancer research, you may choose to conduct in-depth somatic variant analysis on a subset of genes or specific cancer subtype. Using Ion AmpliSeq™ Designer, researchers can simply add or subtract genes from this panel to create your own custom panel. The flexibility and scalability of Ion AmpliSeq™ Solutions spans across the Ion AmpliSeq™ Ready-to-use Panels and Ion AmpliSeq™ Custom Panels.

    For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

    CarrierSeq ™ ECS Kit with Ion 540™ Chips (16 samples/chip) Ion Torrent™

    The Ion Torrent CarrierSeq ECS kits provide all the reagents and materials necessary for a comprehensive, seamless, and flexible next-generation sequencing (NGS) workflow for expanded carrier screening (ECS). When used with an Ion GeneStudio S5 System, the kits enable a simple, end-to-end workflow for the detection of carrier-positive samples by research labs interested in maximizing the identification of carrier status using genomic DNA isolated from blood or saliva samples.The Ion AmpliSeq CarrierSeq ECS Panel included in the kits enables the detection of single nucleotide variants (SNVs), insertion/deletions (indels), and copy number variants (CNVs) associated with 418 inherited disorders in a single assay.

    Benefits of the CarrierSeq ECS kits include:
    • Targeting of all coding regions and intron/exon boundaries of 420 genes implicated in 418 inherited disorders
    • Incorporation into a single NGS assay difficult-to-characterize genes due to homology as a result of paralogues (spinal muscular atrophy (SMN1 and SMN2)), pseudogenes (Gaucher disease (GBA and GBAP1)), 21-hydroxylase deficient congenital adrenal hyperplasia (CYP21A2 and CYP21A1P), and loci (alpha-thalassemia (HBA1 and HBA2))
    • For use with Carrier Reporter, intuitive data analysis software that quickly translates data into results with customizable analysis and reporting options
    • Flexible throughput options—kit formats available for 3, 4, 15, or 16 samples per chip or 6, 8, 30, or 32 samples per run

    Comprehensive content and consolidated assays
    The Ion AmpliSeq CarrierSeq ECS Panel provides comprehensive coverage of common and rare variants to help achieve higher per-disorder detection rate. The panel targets >14,000 amplicons that cover all coding regions of 420 target genes, including intron/exon boundaries, to genotype more than 28,000 SNVs and indels from the ClinVar archive of human variation. The panel also provides robust targeting for CNV analysis to maximize carrier status detection.

    Genetic variants for a number of most prevalent yet serious disorders can be difficult to resolve by NGS assays, and so separate additional stand-alone tests are often required. The Ion AmpliSeq CarrierSeq ECS Panel consolidates such stand-alone assays into a single assay, including difficult-to-sequence genes, such as SMN1 for spinal muscular atrophy, GBA for Gaucher disease, CYP21A2 for 21-hydroxylase deficient congenital adrenal hyperplasia, and HBA1 and HBA2 for alpha thalassemia.

    Streamlined implementation and workflow
    The CarrierSeq ECS kits are an end-to-end solution that includes reagents for target amplification, library preparation, and sequencing with software for data analysis and reporting. The reagents are optimized to work together out of the box and ready to use with the Ion Chef and Ion GeneStudio S5 systems for template preparation and sequencing, respectively, and Carrier Reporter Software for reporting functionality with customizable options.

    Straight-forward and powerful data analysis and reporting
    Carrier Reporter Software is easy to use and designed specifically for carrier screening analysis and reporting. It incorporates the output of special analysis algorithms that address CNV and SNV/indel calling for technically challenging genes such as CYP21A2, GBA, HBA1/2, and SMN1.

    Ridom™ SeqSphere+ Software for Ion Torrent™ Systems (Commercial) Ion Torrent™

    SeqSphere+ Software from Ridom allows for automatic processing and analyzing of microbial sequence data obtained from Ion Torrent™ systems. SeqSphere+ Software enables whole genome microbial typing (MLST+) or traditional MLST sequencing projects. This software is designed both for individual labs and for distributed work-groups (client/server model) to enable easy data sharing. License options are available for 1, 3, or 5 years; 2 or 5 seats; and commercial or academic/government labs.

    Ridom SeqSphere+ Software Features:

    User-friendly: no scripting skills or bioinformatician needed

    Highly automated workflow: download a pre-designed task template or create a custom template, then apply it to the analysis of hundreds of strains with almost no user intervention.

    Single, expanding nomenclature service: worldwide, unique nomenclature service that enables a 'Molecular Typing Esperanto'

    DNA re-sequencing editor: edit and analyze reference-mapped assemblies of Ion Torrent™ data (e.g., MLST, MLST+) with auto-correction of homopolymer-related insertion/deletion errors.

    Sanger data: assemble, edit, and analyze Sanger CE sequencing data (e.g., MLST).

    Analytical tools: select data entries from a comparison table for epidemiologic, evolutionary, or functional analysis. Cluster and visualize data entries by using minimum spanning or UPGMA/neighbor-joining trees.

       --Store, search, retrieve, export, and create reports from your epidemiologic and DNA sequence data stored in an integrated database. Search new sequence entries against stored data.
       --Data fields are compliant with the metadata requirements of the NCBI BioSample. Enables data entry plausibility checks.

    Bacterial typing: typing of bacteria is automatically performed with user-defined quality parameters (e.g., coverage, stop codons) using public or self-defined query libraries and task templates.

    Ridom Community:
       --Rapidly and easily share task templates with other institutions or download them online from the Task Template 'Store'.
       --Option to contribute to a single, worldwide, expanding, publicly-available database of nomenclature and epidemiologic data. Larger (supra) national institutions may obtain their own server for epidemiologic data upon request.

       --Encryption (SSL) of data in transmission
       --Various configurable user roles, user groups, and access controls
       --Audit trail functionality (who, when and what)

    Computer Specifications (Minimum)

    Client computer OS: Windows® 7 64-bit, CPU: Core i5, RAM: 8 GB, HD: 500 GB, Internet connection required to use public Ridom MLST+ Nomenclature Server & Task Template 'Store' (supports working with a private and local nomenclature and to generate custom tasks).

    Server computer OS: Windows® 7/Linux 64-bit (Ubuntu LTS recommended), CPU: Core i5, RAM: 8 GB (48 GB recommended if MIRA de novo assembly done on this server), HD: 2 TB (RAID level 1 or 5 recommended), it is recommended not to use the NGS machine computer as server.

    Ion 510™ Food Protection Chip Kit Thermo Scientific™

    Simplify sample tracking and sequencing with the Ion 510™ Food Protection Chip Kit as a part of  Thermo Scientific™ Next Generation Sequencing (NGS) Food Authenticity Workflow incorporating Ion Torrent™ Next Generation Sequencing technology.

    The Thermo Scientific NGS Food Authenticity Workflow allows identification of meat, plant or fish species present in even the most challenging sample types, protecting your business from food fraud.

    The Food Protection Ion 510 Chip Kit contains eight barcoded chips for sample tracking and sequencing with the Ion GeneStudio S5 Food Protection sequencing system.

    Retainer & Base Set (Standard) for 3500xL Genetic Analyzer, 384 well Applied Biosystems™

    This 384 -well plate Retainer and Base Standard Set is for use with the 3500xL Genetic Analyzer.

    For Research Use Only. Not for use in diagnostics procedures.

    310 Genetic Analyzer Capillary, 47 cm (36 cm wtr - well to read) Applied Biosystems™

    This is an internally uncoated Capillary for the 310 Genetic Analyzer, 47 cm long. Use for genescan and rapid sequencing applications. 100 runs⁄capillary.

    Used With
    2.5 µL Glass Syringe, 310 Running Buffer (10X), POP-4® Polymer for the 310 Genetic Analyzer, POP-6™ Polymer for the 310 Genetic Analyzer.

    For Research Use Only. Not for use in diagnostics procedures.

    GeneArt™ Site-Directed Mutagenesis System Invitrogen™

    The GeneArt® Site-Directed Mutagenesis System provides a state-of-the-art, simple, convenient, and highly efficient means to generate mutations on a target construct in vitro in less than three hours. This system replaces the popular GeneTailor™ Site-Directed Mutagenesis System, and has been completely redesigned to be at the leading edge of commercial site-directed mutagenesis products currently available on the market. This product brings our mutagenesis technology to the next level. Note: A PCR enzyme is not included with the system and must be purchased separately. The recommended enzyme for this kit is AccuPrime™ Pfx DNA Polymerase.

    Powerful – Make substitutions, deletions, or insertions of up to 12 nucleotides in plasmids up to 14 Kb
    Efficient – Enables you to obtain your desired mutant the first time; over 90% correct mutants for a 3 Kb plasmid
    Fast – Have your mutated plasmid DNA in less then 3 hours with the simple, minimal handling protocol
    Versatile – Use plasmids of many sizes, and DNA isolated from any source, with no need for specialized vectors, host strains, or restriction sites

    Simple Creation of Desired Mutants
    Creating mutants with the GeneArt® Site-Directed Mutagenesis System relies on the inherent properties of DNA methylase, high fidelity DNA polymerase, recombination enzymes, and the native McrBC endonuclease of E. coli. Simply incorporate the mutation or mutations (up to 12 nucleotides), that you want into primers, and after PCR, recombination, and transformation you get vectors with only the mutations you desired with up to 90% efficiency. The template vector that you add mutations to can be isolated from any source and up to 14Kb in size. For creating complete constructs, or for joining large pieces of unrelated DNA see our GeneArt® Seamless Cloning and Assembly Kit (cat# A13288) or our GeneArt® High-Order Genetic Assembly System (cat#A13286).

    Optimized Mutagenesis Protocol
    The GeneArt® Site-Directed Mutagenesis System has been optimized for efficiency and simplicity. The DNA methylation and amplification steps are combined into a single reaction with no need for an in vitro DpnI digestion step. After methylation and amplification, a 10 minute in vitro recombination reaction of the amplified PCR products increases the colony output by 3 to 10 fold; resulting in a higher mutagenesis efficiency. A final transformation of the mutated DNA into DH5α™ E.coli cells digests any methylated parental DNA, leaving behind only the intact unmethylated mutagenesis reaction product. No purification steps or additional digestions are needed. Individual colonies can be selected the following day to verify mutations.

    Synthesis Column Filters Applied Biosystems™

    These filters are intended for use with empty synthesis columns to allow users to pack columns manually.

    For Research Use Only. Not for use in diagnostics procedures.

    BigDye™ Terminator v3.1 Cycle Sequencing Kit Applied Biosystems™

    The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

    • Improve the quality of your results for a wide range of sequencing applications
    • Optimized for long read lengths
    • Better dye mobility characteristics
    • Improved performance reading through GT-rich regions
    • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
    • Enhance your productivity and reduce costs

    Get more uniform peak heights and improved accuracy
    With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

    MicroAmp™ Optical Film Compression Pad Applied Biosystems™

    Applied Biosystems® MicroAmp® Optical Film Compression Pads are designed to support a proper thermal seal between the thermal cycler and reaction plates when using adhesive films.

    Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
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