The GeneArt™ Seamless Cloning and Assembly Kit enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction.
The GeneArt™ Site-Directed Mutagenesis System provides a state-of-the-art, simple, convenient, and highly efficient means to generate mutations on a target construct in vitro in less than three hours. This system replaces the popular GeneTailor™ Site-Directed Mutagenesis System, and has been...
The GeneArt™ Site-Directed Mutagenesis PLUS System brings our mutagenesis technology to the next level with the ability to perform single- or multi-site mutations in larger plasmids with greater efficiency and in less time than the competition.
The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between...
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
pUC19 DNA
Thermo Scientific™
Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.
The GeneArt® High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and recombine DNA fragments with high efficiency.
The Invitrogen TrueTag Donor DNA Kit, DDK, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments. Benefits of the TrueTag Donor DNA kits include: • One-step PCR amplification to generate donor...
Thermo Scientific Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 bp) with 12 bp single-stranded complementary 5’-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining.
pUC57 DNA
Thermo Scientific™
Thermo Scientific Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3’-ends that are resistant to E. coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions.
The GeneArt® Type IIs Assembly Kit, Bbs I, provides seamless cloning and assembly of up to 8 DNA fragments by simultaneous cleavage and ligation in a single reaction. The kit uses a technology similar to Golden Gate cloning with the type IIs restriction enzyme Bbs I and can be used to assemble...
The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments.
pUC18 DNA
Thermo Scientific™
Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation.
Thermo Scientific phiX174 was the first DNA virus discovered to have a single-stranded, circular genome. phiX174 DNA is 5386 nt in length. The DNA strand packaged into the virion is termed the “plus” strand.