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DNA Cloning and Mapping and Mutagenesis
A wide variety of kits, systems, DNA, reagents, well plates, consumables, and other products for use with DNA cloning, sequencing, and mapping procedures and/or mutagenesis experiments.
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Bacterial or Yeast Strain: TOP10. Cell Type: Chemically Competent E. coli Cloning Method: Seamless Cloning. Content And Storage: The kit has enough reagents for 20 cloning reactions and a control. The components are: 10X Enzyme Mix and 5X Reaction Buffer, control vector and insert, One Shot™ Chemically Competent TOP10 E. coli, S.O.C. Medium, and a supercoiled control plasmid for E.coli.
A cloning vector is not included in this kit.
For your convenience and flexibility, a ready-to-use linear vector is available separately for 20 cloning reactions (GeneArt™ Linear pUC19L Vector for Seamless Cloning, catalog number A13289).
A cloning vector is not included in this kit.
For your convenience and flexibility, a ready-to-use linear vector is available separately for 20 cloning reactions (GeneArt™ Linear pUC19L Vector for Seamless Cloning, catalog number A13289).
Cloning Method: Seamless cloning. Content And Storage: Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Control Type: Positive Control. For Use With (Application): Seamless Cloning & Genetic Assembly.
Control Type: Positive Control. For Use With (Application): Seamless Cloning & Genetic Assembly.
Cloning Method: Restriction Enzyme⁄ MCS. Concentration: 0.5 μg/μL Sample Type: DNA. Final Product Type: DNA.
Cell Type: DH10B. Content And Storage: Box 1
• 10 x DH10B Competent Cells, 100 μL
• pUC19 DNA, 50 μL, 100 pg/μL
• S.O.C. Medium, 5 mL
Store at -80°C.
Box 2
• Phusion Hot Start II DNA Polymerase, 10 μL, 2 U/μL
• Phusion HF Buffer (5X), 1.5 mL
• dNTP Mix, 10 mM each, 20 μL
• FastDigest DpnI, 20 μL
• Control plasmid (in TE buffer), 5 ng/μL
• Control primer mix (25 μM each of 5' phosphorylated primers), 20 μL
• T4 DNA Ligase, 15 μL
• Rapid Ligation Buffer (5X), 200 μL
Store at -20°C. Format: Kit. Sample Type: PCR Products.
• 10 x DH10B Competent Cells, 100 μL
• pUC19 DNA, 50 μL, 100 pg/μL
• S.O.C. Medium, 5 mL
Store at -80°C.
Box 2
• Phusion Hot Start II DNA Polymerase, 10 μL, 2 U/μL
• Phusion HF Buffer (5X), 1.5 mL
• dNTP Mix, 10 mM each, 20 μL
• FastDigest DpnI, 20 μL
• Control plasmid (in TE buffer), 5 ng/μL
• Control primer mix (25 μM each of 5' phosphorylated primers), 20 μL
• T4 DNA Ligase, 15 μL
• Rapid Ligation Buffer (5X), 200 μL
Store at -20°C. Format: Kit. Sample Type: PCR Products.
For Use With (Application): Cellular Imaging. No. of Reactions: 10 reactions. Quantity: 10 Reactions.
Cloning Method: Seamless cloning. Content And Storage: Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Control Type: Positive Control. For Use With (Application): Seamless Cloning & Genetic Assembly.
Control Type: Positive Control. For Use With (Application): Seamless Cloning & Genetic Assembly.
Bacterial or Yeast Strain: TOP10. Cell Type: Chemically Competent E. coli Cloning Method: Seamless cloning. Content And Storage: Includes master mix, positive control, water, and One Shot TOP10 chemically competent E. coli.
Cloning Method: TOPO™-TA. Constitutive or Inducible System: Constitutive. Delivery Type: Transfection. Format: Kit.
For Use With (Application): Protein Detection and Isolation. No. of Reactions: 10 reactions. Quantity: 10 Reactions.
For Use With (Application): Cellular Imaging. No. of Reactions: 10 reactions. Quantity: 10 Reactions.
Concentration: 0.3 μg/μL Sample Type: DNA. Final Product Type: DNA. For Use With (Application): CloningNucleic Acid Gel Electrophoresis.
Format: Kit. Sample Type: DNA. Enzyme: DNA Polymerase, T4 Polynucleotide Kinase. For Use With (Application): Cloning.
For Use With (Application): Cellular Imaging. Quantity: 10 Reactions.
Antibiotic Resistance Bacterial: Ampicillin (AmpR). Cloning Method: LIC Cloning. Sample Type: PCR Products. For Use With (Application): PCR Cloning.
Cloning Method: Restriction Enzyme⁄ MCS. Concentration: 0.5 μg/μL Sample Type: DNA. Final Product Type: DNA.
Learn More (656)
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How Gibson Assembly® works Homology requirements Choosing your approach Vector selection Competent cell selection, Building large DNA constructs that contain no extraneous sequences is often a challenging task.
Introduction to type IIS restriction enzymes Golden Gate cloning How does Golden Gate Assembly work? Site directed mutagenesis Plasmid-based reverse genetics systems Conclusion , Discovered more than 50 years ago, restriction enzymes are foundational elements of molecular biology and genetic...
Documents & Support (453)
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Can you describe the details of your GeneArt Site-Directed Mutagenesis services?
My DNA oligo seems to have mutations when I sequence the final cloning product. Why would this happen?