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DS-33 GeneScan™ Install Kit Dx with GeneScan™ 600 LIZ™ Size Standard v2.0 Dx Applied Biosystems™

The DS-33 GeneScan™ Installation Kit Dx consists of pooled PCR products labeled with 6-FAM™, VIC™, NED™, and PET™ dyes. To generate the pooled products, CEPH individual 1347-02 genomic DNA is amplified with 8 fluorescent PCR primer pairs, which amplify selected microsatellite loci (as listed in the package product insert). The kit also contains a tube of GeneScan 600 LIZ™ Size Standard v2.0 Dx (Cat. No. A25794).

Ion Xpress™ Plus Fragment Library Kit Ion Torrent™

The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100 ng input DNA. In addition, highly efficient library construction enables the creation of 'amplification-free' libraries from 1 µg input material. This kit allows users to modulate an appropriate fragment insert size depending on desired read length and application.

The Ion Xpress™ Plus Fragment Library Kit contains sample preparation reagents for enzymatic shearing and library construction for up to 20 DNA libraries (depending on input DNA type) for semiconductor sequencing. Store components at -20°C.

The Ion Xpress™ Plus Fragment Library Kit offers you:
• Superior coverage uniformity for diverse sample types using our proprietary Ion Shear™ Plus chemistry
• Lower input requirement with as little as 100 ng starting material
• Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes in as fast as 2 hours
• Significant cost savings by removing the need for ancillary shearing devices
• Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With a turnaround time of as little as 2 hours, the Ion Xpress™ Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your sample(s) of interest possible in less time than ever before. Furthermore, Ion Xpress™ Plus fragment libraries exhibit greater coverage uniformity and higher recovery rates across diverse sample types than other library construction methodologies, so you can be confident of generating the best data in the shortest time.

Ion Torrent Sequencing Made Simpler, Faster, and More Affordable
Ion Shear™ Plus DNA fragmentation technology, included in the Ion Xpress™ Plus kit, obviates the need for costly physical shearing devices for most applications and requires as little as 100 ng of sample, accelerating your path to semiconductor sequencing. This flexible technology provides users the ability to adjust the resulting DNA fragment size to accommodate a range of sequencing read lengths depending on their project requirements. This technology is also amenable to automated library preparation systems such as the AB Library Builder™ System to provide quick answers when you need them most. Ion Shear™ Plus DNA fragmentation technology is not included in the Ion Plus kit. Use the Ion Plus kit if physical shearing methods are preferred.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Ion AmpliSeq™ CarrierSeq™ ECS Panel Ion Torrent™

The Ion AmpliSeq CarrierSeq ECS Panel enables detection of single nucleotide variants (SNVs), insertion/deletions (indels), and copy number variants (CNVs) associated with 418 inherited disorders in a single assay. It is included in the Ion Torrent CarrierSeq ECS kits, which provide all of the reagents and materials necessary for a comprehensive, seamless, and flexible next-generation sequencing (NGS) workflow for expanded carrier screening (ECS). When used with an Ion GeneStudio S5 System, the kits enable a simple, end-to-end workflow for the detection of carrier-positive samples by research labs interested in maximizing the identification of carrier status using genomic DNA isolated from blood or saliva samples.

Benefits of the Ion AmpliSeq CarrierSeq ECS Panel include:
• Targeting of all coding regions and intron/exon boundaries of 420 genes implicated in 418 inherited disorders
• Increased carrier status detection rate through genotyping of SNV, indel, and CNV carrier status by NGS
• Incorporation into a single NGS assay difficult-to-characterize genes due to homology as a result of paralogues (spinal muscular atrophy (SMN1 and SMN2)), pseudogenes (Gaucher disease (GBA and GBAP1)), 21-hydroxylase deficient congenital adrenal hyperplasia (CYP21A2 and CYP21A1P), and loci (alpha-thalassemia (HBA1 and HBA2))

Comprehensive content and consolidated assays
The Ion AmpliSeq CarrierSeq ECS Panel provides comprehensive coverage of common and rare variants to help achieve higher per-disorder detection rate. The panel targets >14,000 amplicons that cover all coding regions of 420 target genes, including intron/exon boundaries, to genotype more than 28,000 SNVs and indels from the ClinVar archive of human variation. The panel also provides robust targeting for CNV analysis to maximize carrier status detection.
vGenetic variants for a number of most prevalent yet serious disorders can be difficult to resolve by NGS assays, and so separate additional stand-alone tests are often required. The Ion AmpliSeq CarrierSeq ECS Panel consolidates such stand-alone assays into a single assay, including difficult-to-sequence genes, such as SMN1 for spinal muscular atrophy, GBA for Gaucher disease, CYP21A2 for 21-hydroxylase deficient congenital adrenal hyperplasia, and HBA1 and HBA2 for alpha thalassemia.

Yfiler™ Plus PCR Amplification Kit Applied Biosystems™

Note: The Yfiler Plus kit is now approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

The Yfiler Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.

The Yfiler Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.

Key features of the Yfiler Plus PCR Amplification Kit:
• Improved discrimination capacity through new, highly discriminating markers
• Improved performance with degraded samples and PCR inhibitors
• Improved genotyping accuracy through expanded allelic ladder and virtual bins
• Concordance with existing data in reference haplotype databases
• Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits

Improved discrimination capacity
The Yfiler Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR Yfiler PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.

More information from challenging casework samples
Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.

Streamlined protocol and increased workflow efficiency
The Yfiler Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
• Blood and buccal samples on treated paper substrates, without the need for sample purification
• Blood samples collected on untreated paper substrates and treated with Prep-n-Go Buffer
• Buccal samples collected on swab substrates and treated with Prep-n-Go Buffer

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Genexus™ Barcodes 1-96 AS Ion Torrent™

The Ion Torrent Genexus Barcodes 1-96 AS kit provides one each of Ion Torrent Genexus Barcodes 1-32 AS, 33-64 AS, and 65-96 AS. When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, it supports flexible, fully automated preparation and normalization of up to 96 Ion AmpliSeq libraries. Each plate is filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-96 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Plate Base (heat seal), 96 well Applied Biosystems™

This is a 96-well Plate Base (Heat Seal) for use with Applied Biosystems® 3730 and 3730xl DNA Analyzers.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems with Human/Mouse/Rat rRNA Depletion Kit Invitrogen™

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. Total RNA can be converted to sequencing-ready library within 6.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Superior rRNA removal
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA
• Preservation of 3‘ end sequence information

The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~ 150 bp for whole transcriptome libraries starting with sample inputs of 100–500 ng of total human, mouse, or rat RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based rRNA depletion and library purification steps, enable completion of the entire workflow in approximately 6.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri Stranded RNA Library Prep Kit with Human/Mouse/Rat rRNA Depletion Kit includes ribosomal RNA (rRNA) depletion reagents to enable a comprehensive view of the transcriptome through superior removal of human, mouse, and rat rRNA. Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related prioduct, the Collibri Library Quantification Kit is recommended for qPCR-based quantifications of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

Prep-n-Go™ Buffer (for buccal swabs) Applied Biosystems™

Prep-n-Go™ Buffer (for buccal swabs) is a best-in-class buffer designed to enable high-quality direct PCR amplification of single source samples collected on buccal swabs and amplified using the AmpFLSTR® Identifiler® Direct PCR Amplification Kit or other Applied Biosystems® STR direct chemistries. This product expands direct PCR amplification capabilities to laboratories that use a wide variety of substrates, and is readily adaptable to both manual and automated workflows. No purification, extraction, or quantification is required, thus enabling laboratories to significantly expand their throughput capabilities, while also maximizing sample integrity and data quality.

Key Features:

• Designed to provide consistent, interpretable peaks above the detection threshold, and high quality, well-balanced STR profiles
• Helps simplify automation requirements and expand throughput capabilities
• Helps maximize sample integrity and data quality
• Developed according to stringent performance standards to enable higher data reproducibility, reduced artifacts, and increased success rates with Applied Biosystems® STR direct amplification solutions

Ion Torrent™ Dual Barcode Kit 1-96 Ion Torrent™

The Ion Torrent Dual Barcode Kit 1-96 provides 96 unique dual-matched barcode adapters specifically designed to enable optimal performance of amplicon libraries and Ion semiconductor sequencers. When used in combination with Ion AmpliSeq and Oncomine assays, the kit enables users to pool up to 96 amplicon libraries to conduct multiplexed sequencing analysis, thereby increasing lab efficiency and reducing sequencing costs per sample. The barcode adapters are provided in 96-well plate format.

Features of the Ion Torrent Dual Barcode Kit 1-96 include:
• Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes to identify very rare clones with high accuracy
• Both sequence- and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Higher throughput and variant calling of rare clones
Multiplexing with dual-barcoded libraries enables cost-effective runs by permitting up to 96 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias and provides superior sensitivity in detecting low-frequency clones with high accuracy.

Sequence- and flow-optimized for increased performance and efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool and require a minimal number of flows to interrogate, resulting in more economical multiplexed sequencing runs.

Robust error correction built in by design
Built-in error correction increases the accuracy of sample assignment.

Oncomine™ BCR IGH SR Assay, RNA Ion Torrent™

The Oncomine BCR IGH-SR Assay, RNA, is a robust, targeted next-generation sequencing (NGS) assay designed for hematology-oncology and minimal residual disease (MRD) monitoring research, pharmacodynamics and biomarker analysis of immunotherapy, as well as infectious and autoimmune disease research. The assay kit includes a single pool of multiplex PCR primers, total RNA-to-cDNA synthesis kit and Ion AmpliSeq library reagents.

The assay accurately identifies and measures clonal expansion of B lymphocytes using total RNA from bone marrow, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh-frozen and formalin-fixed paraffin-embedded (FFPE) research samples. The assay identifies unique B-cell clones through targeting of the highly diverse complementarity-determining region 3 (CDR3) of the B-cell receptor IGH chain from mRNA template via AmpliSeq multiplex framework 3 and joining gene primers. The nucleotide sequence of the IGH CDR3 region serves as a natural barcode to enable clone tracking for MRD clinical research and measurements of B-cell clonal expansion and diversity for pharmacodynamics and vaccine biomarker research. IGH CDR3 region amino acid motifs may reveal signatures of B-cell responses to defined antigens for use as future markers of autoimmune or infectious disease. RNA input improves detection of changes in plasmablast and plasma cell populations following immune challenge or administration of immunomodulatory agents. For DNA samples, please see Cat. No. A45483.

Benefits of the Oncomine BCR IGH SR Assay, RNA, include:
• High sensitivity and exceptionally low limit of detection for rare clone detection and tracking in research samples via dual-barcode indexing and high template capture efficiency of AmpliSeq multiplex PCR
• Compatibility with an array of research sample types, including fresh-frozen and FFPE tissue, bone marrow, whole blood, PBLs, and PBMCs
• Assay primers design to amplify all variable and joining gene alleles in the gold-standard IMGT database
• Ability to generate large libraries with minimal input requirement, facilitating detection of rare clones at a frequency of 10-6 from a single library preparation
• Efficient workflow using the Ion Chef templating system and Ion GeneStudio S5 sequencing system, enabling library-to-results time within 48 hours
• Flexible input requirements ranging from 25 ng to 2 µg
• Streamlined and user-friendly informatics solution with automated clonotyping, clonal lineage analysis, reporting of key repertoire features, and multi-sample analysis capability to enable longitudinal tracking in research samples
• Ultra-low substitution error rate minimizing artifacts arising from sequencing errors, enabling highly accurate assessment of clonal heterogeneity in malignant B-cell research samples of interest

The Oncomine BCR IGH-SR Assay, RNA, uses multiplex PCR primers to generate 110-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements.

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

Genexus™ Barcodes 1-32 AS Ion Torrent™

The Ion Torrent Genexus Barcodes 1-32 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 33-64 AS, 65-96 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-32 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit Ion Torrent™

The Ion AmpliSeq Transcriptome Mouse Gene Expression Assay allows simultaneous gene expression measurement of over 20,000 mouse RefSeq genes in a single assay. Providing the ability to work directly with low input levels of total RNA, the Ion AmpliSeq Transcriptome Mouse Gene Expression Assay is ideal for those interested in obtaining comprehensive and highly sensitive gene expression measurements from a wide range of RNA sources. This particular kit enables manual library preparation with throughput flexibility. For automated production, see the Ion AmpliSeq Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit.

The Ion AmpliSeq Transcriptome Mouse Gene Expression Assay offers:
• Flexible, straightforward library preparation—start with as little as 10 ng of total RNA from tissues, cells, or other sources
• Fast gene expression profiling with simple workflow—go from RNA to data in <2 days with ~45 minutes of total hands-on time with the automated workflow of the Ion GeneStudio S5 and Ion Chef systems
• A complementary software plug-in for automated sequencing analysis that provides gene quantitation and reporting as well as quality control metrics
• Compatibility with Ion Xpress Barcode adapters, allowing for multiplexed sequencing

Leveraging the speed and simplicity of Ion AmpliSeq technology, this ultrahigh-multiplex PCR enrichment approach enables rapid gene expression profiling with minimal hands-on time. This research method provides a faster, simpler, and lower-cost sequencing method that is highly correlated to whole-transcriptome RNA sequencing.

Straightforward library preparation and multiplex sequencing with manual kit
Starting with as little as 10 ng of total RNA, libraries can be prepared in approximately seven hours with only one hour of hands-on time. Total RNA is first processed with the SuperScript VILO cDNA Synthesis Kit (not included), and the resulting cDNA is amplified using Ion AmpliSeq technology to accurately maintain expression levels of all genes.

The resulting libraries are ready for downstream template preparation using the Ion OneTouch 2 or Ion Chef System and sequencing on the Ion GeneStudio S5 or Ion Proton System. Libraries can be barcoded for multiplexing, providing flexibility, reduced cost, and increased throughput, with the recommended 16 libraries per Ion 550 Chip and 8 libraries per Ion 540 or Ion PI chip. Other multiplexing options are also possible, depending on the experimental design and desired level of sensitivity.

Plate Base (heat seal) for 3730/3730xl, 384 well Applied Biosystems™

This is a 384-well Plate Base (Heat Seal) for use with Applied Biosystems® 3730 and 3730xl DNA Analyzers.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems Invitrogen™

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. mRNA can be converted to sequencing-ready library within 4.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detectio
• >98% strand specificity
• Efficient detection of non-coding RNA

The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~150 bp for mRNA or whole transcriptome libraries with sample inputs of 1–25 ng of mRNA-enriched or ribo-depleted RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based library purification steps, enable completion of the entire workflow in approximately 4.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The Collibri Stranded RNA Library Prep Kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related product, the Collibri Library Quantification Kit is recommended for qPCR-based quantification of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Gene fusion detection
• Detection of transcript isoforms

3500 Dx Genetic Analyzer CS2 Install Kit Applied Biosystems™

This kit includes all of the consumables and accessories needed to perform an instrument qualification—install, operational, or/and performance. This kit is specific for the 3500 Dx CS2 instrument and comes with a 50-cm 8-capillary array, POP-6™ polymer, and BigDye® Terminator v1.1 sequencing standard.
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