Shop All DNA Sequencing

Concentration


Product Line


Purity


PrepFiler Express/AutoMate Express™ Validation Applied Biosystems™

This is a performance validation of the PrepFiler Express⁄AutoMate Express™ Forensic DNA Extraction System.

For Research Use Only. Not for use in diagnostics procedures.

Ion Xpress™ Plus Fragment Library Kit for AB Library Builder™ System Ion Torrent™

The Ion Xpress™ Plus Fragment Library Kit for AB Library Builder™ System provides a rapid, flexible, and high-throughput solution for library construction upstream of the semiconductor sequencing workflow. This ready-to-use reagent cartridge simplifies next-generation sequencing by enabling automated DNA fragment library preparation for the Personal Genome Machine® and soon the Proton™ sequencing systems.

This kit contains sample preparation reagents for enzymatic shearing and library construction of 13 DNA libraries for semiconductor sequencing. Use of this reagent cartridge requires the addition of library adapters (sold separately).

This library kit offers:

Simplicity and convenience of automated library preparation upstream of semiconductor sequencing
Superior coverage uniformity for diverse sample types using our proprietary Ion Shear™ Plus chemistry
Lower input requirement providing the ability to make amplification-free libraries from as little as 50 ng of starting DNA
Rapid and flexible workflow producing Ion fragment libraries containing user-selectable and automatically size-selected insert sizes of 100–400 bp in as little as 2 hours
Significant cost savings by removing the need for ancillary shearing devices and size-selection gels
Scalable methodology to meet high-throughput sequencing requirements

With its plug-n-play modular reagent design, this revolutionary kit automates all steps of library creation, including both DNA fragmentation and library size selection, in as little as 2 hours with limited set-up time and minimal subsequent user-intervention. DNA fragmentation is automated with enhanced, proprietary Ion Shear™ DNA fragmentation chemistry to achieve user-selectable inserts of 100–400 bp in length. Size selection of the libraries is performed automatically without the expense or effort of gel-based methods. Alternatively, libraries can be manually size-selected using conventional methods after the automated run. Starting with 50–1000 ng of input DNA, the kit yields significantly higher amounts of library than current manual methods, eliminating the need for PCR amplification (i.e., 'amplification-free' libraries) for most sequencing needs.

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With the ability to prepare up to 13 libraries in a little over 2 hours, the Ion Xpress™ Plus Fragment Library Kit for AB Library Builder™ System is a key step towards automation of the Ion sequencing workflow, making comprehensive analyses of your sample(s) of interest more convenient than ever before. Furthermore, Ion Xpress™ Plus fragment libraries generated using the AB Library Builder™ system exhibit the superb coverage uniformity typically seen with proprietary Ion Shear™ DNA fragmentation chemistry and higher recovery rates than other library construction methodologies. So you can be confident of generating the best data in the shortest time, without the hassle or expense of manual shearing and size selection.

Ion Torrent Sequencing Made Simpler, Faster, and More Affordable
Ion Shear™ Plus DNA fragmentation technology eliminates the need for costly physical shearing devices for most applications and requires as little as 50 ng of sample, accelerating your path to semiconductor sequencing. This flexible technology also provides users the ability to adjust fragmentation and size-selection sizes to accommodate a range of sequencing read lengths depending on their project requirements. This automated library preparation solution provides fast and scalable workflows for sequencing projects requiring low- to high-throughput processing capabilities.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Ion AmpliSeq™ Comprehensive Cancer Panel Ion Torrent™

The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. View the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (PDF).

The Ion AmpliSeq™ technology allows ultra-high multiplex PCR combined with targeted sequencing library construction in just 16 hours for over 400 genes. Utilizing only 10 ng of input DNA per primer pool for a total of 40 ng of input DNA, analysis of restricted samples like FFPE samples is possible. The Ion AmpliSeq™ Comprehensive Cancer Panel is optimized for library construction with the Ion AmpliSeq™ Library Kit 2.0. Libraries are then ready for template preparation on the Ion OneTouch™ System and sequencing on the Ion PGM™ Sequencer. The Ion AmpliSeq™ Comprehensive Cancer Panel is not compatible with Ion Ampliseq™ 2.0 Beta Kits.

Features and Benefits:

• Broad survey of 409 key genes in a simple PCR reaction, no additional capital equipment required
• Unmatched plexy of 16,000 primer pairs in only four pools with Ion AmpliSeq™ technology
• Low DNA input of only 40 ng DNA and short amplicons enable FFPE samples and needle biopsies
• Start comprehensive genomic studies quickly with pre-designed primer pools
• Simplify analysis and annotation of variants with Ion Reporter™ software

Comprehensive Gene Content Including Genes from Hotspot Panel
Ion Torrent has designed the most extensive cancer panel available for sequencing with Ion PGM™ and, soon, Ion Proton™ Sequencers. Working with key cancer researchers and reviewing literature and databases, the Ion AmpliSeq™ Comprehensive Cancer Panel was designed to target all exons of key tumor suppressor genes and oncogenes most frequently cited and most frequently mutated. Strategically designed to interrogate CDS and splice variants across multiple gene families simultaneously, pathway-based gene selection profiles mutational spectrum in cancer driver genes and drug targets, along with signaling cascades, apoptosis, DNA repair, transcription regulators, inflammatory response, and growth factor genes in a single assay. Additionally, all genes from the focused Ion AmpliSeq™ Cancer Panel are included in the Ion AmpliSeq™ Comprehensive Cancer Panel providing corresponding complementary data.

Unmatched Level of Multiplex PCR
The Ion AmpliSeq™ Comprehensive Cancer Panel is comprised of four primer pools totaling almost 16,000 primer pairs covering 409 genes-that's approximately 4,000 primer pairs in each pool! Used in conjunction with the Ion AmpliSeq™ Library Kit 2.0, the Ion AmpliSeq™ Comprehensive Cancer Panel maintains high uniformity and specificity at this high plexy, advancing multiplex PCR to new levels.

Over 400 Genes, Only 40 ng DNA Required
While other target selection methods typically require micrograms of DNA input, the Ion AmpliSeq™ Comprehensive Cancer Panel uses only 40 ng DNA. This breakthrough in low DNA input requirement enables the interrogation of restricted samples like formalin-fixed, paraffin-embedded samples (FFPE) and fine needle aspiration biopsies (FNAB). Additionally, shorter amplicon design (average 155 bp) allows for compatibility with degraded samples.

Simplified Target Selection Without Primer Design
With pre-designed primers in the Ion AmpliSeq™ Comprehensive Cancer Panel, researchers can bypass gene selection, primer design, and optimization, and advance straight to generating results for a broad survey of hundreds of genes in key research samples. To further focus your cancer research, you may choose to conduct in-depth somatic variant analysis on a subset of genes or specific cancer subtype. Using Ion AmpliSeq™ Designer, researchers can simply add or subtract genes from this panel to create your own custom panel. The flexibility and scalability of Ion AmpliSeq™ Solutions spans across the Ion AmpliSeq™ Ready-to-use Panels and Ion AmpliSeq™ Custom Panels.

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Ion AmpliSeq™ Direct FFPE DNA Kit Ion Torrent™

The Ion AmpliSeq™ Direct FFPE DNA Kit enables the preparation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissues for library construction using the Ion AmpliSeq protocol, without the need for deparaffinization or DNA purification. This kit is suitable for FFPE tissue sections up to 10 µm thick.

Features of the Ion AmpliSeq Direct FFPE DNA Kit include:
• Simple one-tube 2-step protocol that minimizes sample loss
• Efficient 30-minute workflow with only 10 minutes of hands-on time
• Ideal for FFPE DNA samples with limited DNA amount
• No deparaffinization of FFPE sections required
• No column or bead-based DNA purification required
• Ion AmpliSeq library preparation with as little as 1 ng input DNA
• Uracil-D-glycosylase treatment to remove deaminated cytosines is an optional step

Extraction of DNA from samples with limited DNA amount
Archived tissue samples can provide clinically relevant information, but it can be difficult to isolate the small amount of nucleic acid they contain. Deparaffinization of FFPE tissue sections and purification of the DNA in these samples can be a complicated, multi-step procedure requiring considerable hands-on time, and the associated sample-loss makes it an unsuitable approach for processing FFPE DNA samples with limited DNA amounts.

A simpler and faster method
The Ion AmpliSeq Direct FFPE DNA Kit provides a user-friendly protocol that eliminates the need for deparaffinization of FFPE tissue sections and purification of the DNA. From an FFPE section mounted on a glass slide, the area of interest is removed using Transfer Solution and pipetted into a PCR tube or 96-well plate. After the addition of Direct Reagent, the sample is incubated at 65°C for 15 minutes. The FFPE DNA is now ready for the Ion AmpliSeq™ Library Preparation protocol. The prepared FFPE DNA can be quantified using the Qubit™ HS DNA Quantitation Kit, if necessary. The simplicity of this protocol eliminates the need for complicated deparaffinization and DNA extraction procedures, and libraries can be prepared either manually or with the automated Ion Chef™ System.

Collibri™ PS DNA Library Prep Kit for Illumina Systems, with UD indexes (Set A, 1-24) Invitrogen™

The Invitrogen Collibri PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PS DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success
• Includes all necessary components in a single kit
• Offers consistent, even coverage of variable GC regions from inputs ranging from 1 to 1,000 ng
• Offers improved coverage of challenging genomic regions compared to already available library prep technologies
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PS DNA Library Prep Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PS DNA Library Prep Kit consistently offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from a wide range of inputs (1 ng to 1 µg) of physically sheared double-stranded DNA (see figure below). Additionally, a PCR-free version is offered for inputs starting at 500 ng DNA. The entire library preparation protocol takes about 3.5 hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

ONC ION AMPLISEQ FOR CHEF, DL8 Ion Torrent™

This is a replicate of the existing AmpliSeq SKU A29024 for revenue under the CS4 PL to be bundled with Oncomine tumor specfic panels.

dGTP BigDye™ Terminator v1.0 Ready Reaction Cycle Sequencing Kit Applied Biosystems™

dGTP BigDye® Terminator Cycle Sequencing Kit with the AmpliTaq® DNA Polymerase, FS. This Ready Reaction Kit uses the same dye set as the BigDye® v1.0 kits.

The dGTP BigDye® Terminator Cycle Sequencing Kit's fluorescent dideoxy terminator chemistry is optimized for sequencing through GT- and G-rich templates.

• Ensure reliable results with difficult regions and finishing applications.
• Sequence templates with challenging sequence contexts.
• Sequence problematic templates that standard chemistries cannot.

Sequence Challenging Templates
The dGTP BigDye® Terminator Kit is ideal for working with GT- and G-rich and other difficult-to-sequence templates. The dGTP BigDye® Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples.

Optimized for GT-Rich Templates
Obtain higher success rate even with your difficult GT-rich templates. By using the same chemistries as the standard BigDye® Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye® Terminator Kit provides proven performance, optimized for GT-rich and other difficult-to-sequence templates.

Easily Integrate into Your Workflow
dGTP BigDye® Terminator Kits require no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.

Ion Universal Library Quantitation Kit Thermo Scientific™

The Ion Universal Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input generated by the Ion 16STM Metagenomics Kit for Ion Torrent™ semiconductor sequencing. This kit provides the TaqMan® assay, TaqMan® Universal Master Mix, and library standard needed to quantify femtomolar amounts of Ion fragment libraries. With cycling conditions that allow both fast and standard cycling, the Ion Universal Library Quantitation Kit supports most real-time platforms. This real-time PCR kit enables greater accuracy and specificity compared to non-PCR or non-probe-based methods, delivering simplicity and speed to the Ion PGM™ System sequencing workflow.

The Ion Universal Library Quantitation Kit enables:
• Precision—TaqMan® assays enable greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods
• Sensitivity—the protocol supports quantitation of femtomolar amounts of fragment libraries
• Simplicity—the validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation
• Versatility—the highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range
• Efficiency—qPCR-based methods use less material for quantification

This kit includes:
• A TaqMan® assay designed for Ion library adaptors
• TaqMan® Universal PCR Master Mix
• An adapted E. coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ~190 bp in size

Note: In addition to use with the Ion 16S™ Metagenomics Kit workflow, the Ion Universal Library Quantitation kit is also compatible with sequencing workflows for amplicons generated using any master mix.

MicroSEQ™ ID Microbial Identification Software v3.0 Lite Applied Biosystems™

MicroSEQ® ID Microbial Identification Software allows you to easily identify and classify unidentified bacterial or fungal sequences by comparing them to a validated microbial library. The Lite version of the software can be used on a stand-alone PC (not connected to the genetic analyzer) for simple analyses.

• A validated library of over 2300 microbial sequences help ensure you get the answers you need
• Automatically identify unknown specimens
• Run plates on Applied Biosystems® 3500 Series Genetic Analyzers directly via the MicroSEQ® ID Software
• MicroSEQ® system is a complete integrated solution, including security, audit, and e-signature features
• Qualification and validation support

Confidently Identify Microbes
The MicroSEQ® system comprises bacterial libraries of 500 bp sequences and over 2300 validated entries--including extensive coverage of gram-negative nonfermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. Another library containing more than 1100 entries is available for fungal identification. To help ensure accurate identification of the species of your sequences, we rigorously validate all library entries. And unlike other widely available databases, MicroSEQ® libraries contain only correctly identified organisms and quality-controlled sequences derived from type strains of preeminent culture collections. The libraries also support sequence polymorphisms, and include bacterial Category A biothreat agents.

Automatically Identify Unknown Specimens
Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The new Auto-ID feature in MicroSEQ® ID Software v3.0 uses a set of editable parameters to automatically identify unknown specimens. The results table is displayed in the report, and an editable comments field allows additional information or manual identifications to be entered into the same table.

Run Plates on 3500/3500xL Instruments Directly via the MicroSEQ® ID Software
Applied Biosystems® 3500 Series Genetic Analyzer users are now able to run plates on these instruments directly via the MicroSEQ® ID Software v3.0 user interface, without needing Autoanalysis Manager. A single software wizard enables you to set up projects, specimens, and plates; adjust the plate layout via drag and drop; start and monitor the run; and review results.

The MicroSEQ® System: A Complete, Integrated Solution
Streamlining every step in microbial identification, the Applied Biosystems® MicroSEQ® system combines the advantages of MicroSEQ® ID Software with easy to use PrepMan® Ultra sample preparation reagents and protocols, MicroSEQ® bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. The MicroSEQ® ID Software also includes security, audit, and electronic signature features enabling the software to smoothly integrate into your workflow.

Qualification and Validation Support
To help ensure seamless integration into your laboratory, from installation to routine operation, we offer comprehensive installation and operational qualification (IQ/OQ) and validation support services.

For Research Use Only. Not for use in diagnostics procedures.

Oncomine™ Comprehensive Assay v3M Ion Torrent™

The Oncomine Comprehensive Assay v3 is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 161 unique genes to help inform drug discovery research and clinical trial research programs. It provides the reagents for library construction and four pools—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Designed to help you go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion S5 System with the Ion 540 Chip to enable up to 8 samples (7 samples and one no-template control) per run.

When combined with Oncomine Knowledge Reporter Software, you can create reports that align labels, guidelines, and clinical trials to your results so you can focus on key drivers of cancer in your research.

Key features of the assay are:
• Enables analysis of variants across 161 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool (40 ng total) isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

From sample to answer
The Oncomine Comprehensive Assay is part of an NGS workflow that helps researchers to filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

The workflow has been adopted by large-scale national clinical trial research programs including NCI-MATCH (United States) and LC-SCRUM (Japan), and includes:
• Proven Ion AmpliSeq chemistry
• Automated library and template prep on the Ion Chef System
• Scalable sequencing on the Ion S5 Sytem
• Optimized Oncomine informatics

Multiple configurations
The Oncomine Comprehensive Assay v3 comes in the following configurations to match your laboratory needs:

Manual library preparation configuration:
Oncomine Comprehensive Library Assay v3M
This configuration contains all the reagents needed to prepare libraries for 24 or 96 samples:
    • Oncomine Comprehensive Panel v3M DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3M RNA—contains the RNA primer pools
    • Ampliseq Library Plus reagents—allow you to manually create your library
Ion Chef automated library preparation configuration:
Oncomine Comprehensive Library Assay v3C
This configuration contains all the reagents needed to prepare libraries for 32 samples:
    • Oncomine Comprehensive Panel v3C DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3C RNA—contains the RNA primer pools
    • Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System


Collibri™ PS DNA Library Prep Kit for Illumina Systems, with CD indexes Invitrogen™

The Invitrogen Collibri PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PS DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success
• Includes all necessary components in a single kit
• Offers consistent, even coverage of variable GC regions from inputs ranging from 1 to 1,000 ng
• Offers improved coverage of challenging genomic regions compared to already available library prep technologies
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PS DNA Library Prep Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PS DNA Library Prep Kit consistently offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from a wide range of inputs (1 ng to 1 µg) of physically sheared double-stranded DNA (see figure below). Additionally, a PCR-free version is offered for inputs starting at 500 ng DNA. The entire library preparation protocol takes about 3.5 hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

DS-33 Matrix Standard Set for the 310 Genetic Analyzer Applied Biosystems™

The dye-labeled oligonucleotides included in the Matrix Standard Set DS-33 [6-FAM™, VIC®, NED™, PET®, and LIZ®] are used to generate the "multi-component matrix" required for the ABI PRISM® 310 Genetic Analyzer under denaturing applications.

The 310 instrument Data Collection Software then utilizes the multicomponent matrix to automatically correct the spectral overlap in samples labeled with DS-33 dyes. Matrix standards do not need to be run with every set of sample injections. This set of five matrix standards only need to be run once to generate a matrix file which is then applied to samples run under similar conditions. For more information on the use of matrix standards, please refer to the instrument user’s manual.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ ID Microbial Identification Software v3.1 Applied Biosystems™

MicroSEQ ID Microbial Identification Software v3.1 allows you to easily identify and classify unidentified bacterial or fungal sequences by comparing them to a validated microbial library. This version of the software does not include the bacterial and fungal sequence libraries.

• Automatically identify unknown specimens against a validated library of over 3000 microbial sequences help ensure you get the answers you need (libraries not included with this version of the software)
• Run plates on Applied Biosystems 3500 Series genetic analyzers directly via the MicroSEQ ID software
• The MicroSEQ system is a complete integrated solution, including security, audit, and e-signature features
• Qualification and validation support are available

Confidently identify microbes
The MicroSEQ ID sequence libraries consist of bacterial libraries of 500 bp 16S rDNA sequences and over 2300 validated entries, including extensive coverage of gram-negative non-fermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus, as well as a fungal library containing more than 1100 entries. To help ensure accurate identification of the species of your sequences, we rigorously validate all library entries. And unlike other widely available databases, MicroSEQ libraries contain only correctly identified organisms and quality-controlled sequences derived from type strains of preeminent culture collections. The libraries also support sequence polymorphisms, and include bacterial Category A biothreat agents.

Automatically identify unknown specimens
Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The Auto-ID feature in MicroSEQ ID software uses a set of editable parameters to automatically identify unknown specimens. The results table is displayed in the report, and an editable comments field allows additional information or manual identifications to be entered into the same table.

Run plates on 3500/3500xL instruments directly via the MicroSEQ ID software
Applied Biosystems 3500 Series genetic analyzer users are now able to run plates on these instruments directly via the MicroSEQ ID software v3.1 user interface, without needing Autoanalysis Manager. A single software wizard enables you to set up projects, specimens, and plates; adjust the plate layout via drag and drop; start and monitor the run; and review results.

The MicroSEQ system: A complete, integrated solution
Streamlining every step in microbial identification, the MicroSEQ system combines the advantages of MicroSEQ ID software with easy-to-use PrepMan Ultra sample preparation reagents and protocols, MicroSEQ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. The MicroSEQ ID software also includes security, audit, and electronic signature features enabling the software to smoothly integrate into your workflow.

Qualification and validation support
To help ensure seamless integration into your laboratory, from installation to routine operation, we offer comprehensive installation and operational qualification (IQ/OQ) and validation support services.

ONC ION AMPLISEQ FOR CHEF, 4x DL8 Ion Torrent™

This is a replicate of the existing AmpliSeq SKU A89024 for revenue under the CS4 PL to be bundled with Oncomine tumor specfic panels.

Oncomine™ Lung cfDNA Assay Ion Torrent™

The Oncomine™ Lung cfDNA Assay is part of a complete research solution to detect lung tumor-derived DNA (ctDNA) in cfDNA. It provides the reagents for library construction and a single pool of multiplex PCR primers for preparation of amplicon libraries from cell-free DNA (cfDNA) obtained from the plasma fraction of a single tube of whole blood. The assay enables researchers to analyze single nucleotide variants and short indels that are frequently mutated in non-small cell lung cancer (NSCLC). Eleven genes and >150 hotspots are covered (Genes: ALK, BRAF, EGFR, ERBB2, KRAS, MAP2K1, MET, NRAS, PIK3CA, ROS1, and TP53). Through the use of proprietary sequencing technology, a limit of detection (LOD) down to 0.1% can be achieved.

Note: Target and hotspot BED files and User Guide document for cfDNA Variant Analysis with Torrent Suite Software 5.2 can be found below under 'Documents'.

• Optimized amplicon design for short cfDNA (~170 bp)
• Detection of both single nucleotide variants (SNVs) and short indels down to 0.1% LOD
• Optimized targeted assay design allows highly multiplexed next-generation sequencing (NGS), reducing sequencing costs
• Very high sensitivity and specificity, enabling extremely low levels of detection with minimal false positives
• Efficient workflow, from a single tube of blood to results in just 2 days

Why are pathologists and oncologists interested in liquid biopsy clinical cancer research? Liquid biopsies offer several advantages over conventional solid tumor samples:
• Less invasive to obtain, enabling tumor content to be sampled multiple times
• Lower cost compared to traditional tissue samples
• Faster turnaround time from sample to results
• Help capture more of the heterogeneity of the tumor
• May lead to improvements in standard of care in the future

The entire workflow (figure below) from isolation of cfDNA using the MagMAX™ Cell-Free DNA Isolation Kit to analysis of samples can be accomplished in just 2 days using the Ion Chef™ System and the Ion S5™ XL sequencing system.

Unlike other technologies with low LODs (1-5%), the Oncomine Lung cfDNA Assay has a flexible detection limit down to 0.1% or 1 mutant copy in a background of 1,000 wild-type copies. To achieve 0.1% LOD, 20 ng of input cfDNA is required. Lower amounts of cfDNA can be used, but the resulting LOD will be higher depending on the input amount.

Simplicity, speed, and scalability
The Oncomine Lung cfDNA Assay enables cancer genetic studies from as little as 1 ng of input cfDNA for targeted library construction. The cfDNA assay uses standard PCR equipment and two simple PCR reactions, one for sequencing and the second to amplify the library for high multiplex PCR-based target selection, which minimizes the need for extensive capital investment. Additionally, the Oncomine Lung cfDNA Assay is compatible with FFPE samples for possible concordance studies. Total time to targeted libraries is typically just 3.5 hours. Scalability and flexibility are achieved using the Tag Sequencing Barcode Set 1-24
Results per page
    spinner