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MicroSEQ™ ID Microbial Identification Software v3.0 Bundle for 3500 Instrument Applied Biosystems™

MicroSEQ® ID Microbial Identification Software allows you to easily identify and classify unidentified bacterial or fungal sequences by comparing them to a validated microbial library. This bundled version includes the bacterial and fungal sequence libraries.

A validated library of over 2300 microbial 16S rDNA sequences help ensure you get the answers you need

• Automatically identify unknown specimens
• Run plates on Applied Biosystems® 3500 Series genetic analyzers directly via the MicroSEQ® ID Software
• MicroSEQ® system is a complete integrated solution, including security, audit, and e-signature features
• Qualification and validation support

Confidently Identify Microbes
The MicroSEQ® system comprises bacterial libraries of 500 bp 16S rDNA sequences and over 2300 validated entries--including extensive coverage of gram-negative nonfermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. A fungal library containing more than 1100 entries is also included for fungal identification. To help ensure accurate identification of the species of your sequences, we rigorously validate all library entries. And unlike other widely available databases, MicroSEQ® libraries contain only correctly identified organisms and quality-controlled sequences derived from type strains of preeminent culture collections. The libraries also support sequence polymorphisms, and include bacterial Category A biothreat agents.

Automatically Identify Unknown Specimens
Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The new Auto-ID feature uses a set of editable parameters to automatically identify unknown specimens. The results table is displayed in the report, and an editable comments field allows additional information or manual identifications to be entered into the same table.

Run Plates on 3500/3500xL Instruments Directly via the MicroSEQ® ID Software
Applied Biosystems® 3500 Series Genetic Analyzer users are now able to run plates on these instruments directly via the MicroSEQ® ID Software user interface, without needing Autoanalysis Manager. A single software wizard enables you to set up projects, specimens, and plates; adjust the plate layout via drag and drop; start and monitor the run; and review results.

The MicroSEQ® System: A Complete, Integrated Solution
Streamlining every step in microbial identification, the Applied Biosystems® MicroSEQ® system combines the advantages of MicroSEQ® ID Software with easy to use PrepMan® Ultra sample preparation reagents and protocols, MicroSEQ® bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. The MicroSEQ® ID Software also includes security, audit, and electronic signature features enabling the software to smoothly integrate into your workflow.

Qualification and Validation Support
To help ensure seamless integration into your laboratory, from installation to routine operation, we offer comprehensive installation and operational qualification (IQ/OQ) and validation support services.

DS-33 GeneScan™ Install Kit Dx with GeneScan™ 600 LIZ™ Size Standard v2.0 Dx Applied Biosystems™

The DS-33 GeneScan™ Installation Kit Dx consists of pooled PCR products labeled with 6-FAM™, VIC™, NED™, and PET™ dyes. To generate the pooled products, CEPH individual 1347-02 genomic DNA is amplified with 8 fluorescent PCR primer pairs, which amplify selected microsatellite loci (as listed in the package product insert). The kit also contains a tube of GeneScan 600 LIZ™ Size Standard v2.0 Dx (Cat. No. A25794).

Ion Xpress™ Plus Fragment Library Kit Ion Torrent™

The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100 ng input DNA. In addition, highly efficient library construction enables the creation of 'amplification-free' libraries from 1 µg input material. This kit allows users to modulate an appropriate fragment insert size depending on desired read length and application.

The Ion Xpress™ Plus Fragment Library Kit contains sample preparation reagents for enzymatic shearing and library construction for up to 20 DNA libraries (depending on input DNA type) for semiconductor sequencing. Store components at -20°C.

The Ion Xpress™ Plus Fragment Library Kit offers you:
• Superior coverage uniformity for diverse sample types using our proprietary Ion Shear™ Plus chemistry
• Lower input requirement with as little as 100 ng starting material
• Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes in as fast as 2 hours
• Significant cost savings by removing the need for ancillary shearing devices
• Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With a turnaround time of as little as 2 hours, the Ion Xpress™ Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your sample(s) of interest possible in less time than ever before. Furthermore, Ion Xpress™ Plus fragment libraries exhibit greater coverage uniformity and higher recovery rates across diverse sample types than other library construction methodologies, so you can be confident of generating the best data in the shortest time.

Ion Torrent Sequencing Made Simpler, Faster, and More Affordable
Ion Shear™ Plus DNA fragmentation technology, included in the Ion Xpress™ Plus kit, obviates the need for costly physical shearing devices for most applications and requires as little as 100 ng of sample, accelerating your path to semiconductor sequencing. This flexible technology provides users the ability to adjust the resulting DNA fragment size to accommodate a range of sequencing read lengths depending on their project requirements. This technology is also amenable to automated library preparation systems such as the AB Library Builder™ System to provide quick answers when you need them most. Ion Shear™ Plus DNA fragmentation technology is not included in the Ion Plus kit. Use the Ion Plus kit if physical shearing methods are preferred.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit & Prep-n- Go™ Buffer, for buccal swabs Applied Biosystems™

The AmpFLSTR Identifiler Direct PCR Amplification Kit & Prep-n-Go Buffer (for Buccal Swabs) combines the features of our AmpFLSTR Identifiler Direct PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for Buccal Swabs). The Identifiler Direct kit enables direct PCR amplification of the same 16 loci included in our standard Identifiler kit, while eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Prep-n-Go Buffer (for Buccal Swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

Key Features:

• Enables the direct amplification of buccal swab samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction?purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Identifiler kit, which:

• Maximizes concordance with STR profiles generated with other AmpFLSTR kits including the Identifiler, Identifiler Plus, Profiler Plus, Profiler Plus ID, SGM Plus and COfiler Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

SOLiD™ EZ Bead™ E80 System Consumables, for TargetSeq™-enriched samples Applied Biosystems™

SOLiD™ EZ Bead™ E80 System Consumables for TargetSeq™, 4-pack, contains all necessary reagents and consumables for performing four E80 EZ Bead™ system runs for TargetSeq™-enriched samples, except the SOLiD™ EZ Bead™ Oil Pack Kit, which can be purchased separately.

Included in the kit:

4 SOLiD™ EZ Bead™ Emulsifier E80 Reagents Kits
4 SOLiD™ EZ Bead™ Emulsifier E80 Accessories Kits
2 SOLiD™ EZ Bead™ Amplifier E80 Accessories Kits
4 SOLiD™ EZ Bead™ Enricher Buffer Reagents Kits
4 SOLiD™ EZ Bead™ Enricher E80 Reagents Kits
4 SOLiD™ EZ Bead™ Enricher Accessories Kits

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Genexus™ Barcodes 1-32 AS Ion Torrent™

The Ion Torrent Genexus Barcodes 1-32 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 33-64 AS, 65-96 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-32 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Plate Base (septa seal) for 3730/3730xl, 384 well Applied Biosystems™

This is a 384-well Plate Base (Septa Seal) for use with Applied Biosystems® 3730 and 3730xl DNA Analyzers.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems Invitrogen™

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. mRNA can be converted to sequencing-ready library within 4.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detectio
• >98% strand specificity
• Efficient detection of non-coding RNA

The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~150 bp for mRNA or whole transcriptome libraries with sample inputs of 1–25 ng of mRNA-enriched or ribo-depleted RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based library purification steps, enable completion of the entire workflow in approximately 4.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The Collibri Stranded RNA Library Prep Kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related product, the Collibri Library Quantification Kit is recommended for qPCR-based quantification of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Gene fusion detection
• Detection of transcript isoforms

Yfiler™ Plus PCR Amplification Kit Applied Biosystems™

Note: The Yfiler Plus kit is now approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

The Yfiler Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.

The Yfiler Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.

Key features of the Yfiler Plus PCR Amplification Kit:
• Improved discrimination capacity through new, highly discriminating markers
• Improved performance with degraded samples and PCR inhibitors
• Improved genotyping accuracy through expanded allelic ladder and virtual bins
• Concordance with existing data in reference haplotype databases
• Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits

Improved discrimination capacity
The Yfiler Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR Yfiler PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.

More information from challenging casework samples
Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.

Streamlined protocol and increased workflow efficiency
The Yfiler Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
• Blood and buccal samples on treated paper substrates, without the need for sample purification
• Blood samples collected on untreated paper substrates and treated with Prep-n-Go Buffer
• Buccal samples collected on swab substrates and treated with Prep-n-Go Buffer

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Oncomine™ Myeloid Research Assay GX Ion Torrent™

The Ion Torrent Oncomine Myeloid Assay GX is a comprehensive targeted next-generation sequencing (NGS) assay designed for sensitive detection of myeloid disorder-associated DNA mutations and fusion transcripts in blood and bone marrow samples.

This assay is compatible with the Genexus Integrated Sequencer, which performs library preparation, sequencing, analysis, and reporting in an automated sample-to-result workflow. Depending on the workflow, results can be obtained in as little as a single day.

The Oncomine Myeloid Assay GX includes the following features:
• Comprehensive coverage of key DNA mutations and fusion transcripts associated with myeloid disorders
• Automated sample-to-report workflow on the Genexus sequencer in less than a day
• Sequence up to eight samples (DNA & RNA) per lane on an Ion Torrent GX5 Chip in a single run
• Less than 15 minutes hands-on time
• Detection of somatic variants down to 5% allele frequency

The Oncomine Myeloid Assay GX is designed to assist in the understanding of myeloid cancer. The DNA panel is comprised of 40 key genes while the RNA panel includes a broad fusion panel of 29 driver genes, covering the most relevant targets associated with major myeloid disorders, including acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).

The multiplex primer design leverages Ion AmpliSeq technology to generate results from multiple samples in a single run. Sequencing results are automatically analyzed by Genexus software using an optimized assay-specific analysis workflow.

The assay kit includes three pools of Ion AmpliSeq oligonucleotide primers (a 2-pool DNA panel and 1-pool RNA panel) and Genexus Strips 1 & 2-AS library reagents, enough to sequence up to 32 samples on the Genexus Integrated Sequencer. One end-to-end sequencing run uses one lane on the Ion Torrent GX5 Chip and accommodates up to eight samples. Additional Genexus sequencer reagents and supplies must be ordered separately.

GeneScan™ 1000 ROX™ dye Size Standard Applied Biosystems™

GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems® fluorescence-based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 1000 ROX™ Size Standard is designed for sizing DNA fragments in the 47–946 bp range and provides labeled fragments of 47, 51, 55, 82, 85 93 99, 126, 136, 262, 293, 317, 439, 557, 692, 695 and 946 bases. Each of the DNA fragments is labeled with ROX™ fluorophore, which results in a single peak when run under non-denaturing conditions.

Note: Under denaturing conditions using POP-4® polymer, fragment lengths between 100 and 539 bp can be determined (the linear range of the separation with respect to length vs. scan number).

Ion 540™ Chip Kit Ion Torrent™

The Ion 540™ Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5™ and Ion S5™ XL Sequencing Systems. The Ion 540 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of as little as 2.5 hours for 200 bp sequencing.

The Ion 540 Chip Kit offers:

• Sample tracking functionality from an integrated barcode
• A rapid run time of as little as 2.5 hours for 200 bp sequencing, generating 60–80 million reads
• Support for a variety of research applications, including sequencing of up to three human exomes, transcriptome profiling, and targeted resequencing of gene panels
• Easy handling and loading of templated products for sequencing
• Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems.
Use this kit with the Ion 540 Kit-Chef and the Ion 540 Kit-OT2.

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems with Human/Mouse/Rat rRNA Depletion Kit Invitrogen™

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. Total RNA can be converted to sequencing-ready library within 6.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Superior rRNA removal
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA
• Preservation of 3‘ end sequence information

The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~ 150 bp for whole transcriptome libraries starting with sample inputs of 100–500 ng of total human, mouse, or rat RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based rRNA depletion and library purification steps, enable completion of the entire workflow in approximately 6.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri Stranded RNA Library Prep Kit with Human/Mouse/Rat rRNA Depletion Kit includes ribosomal RNA (rRNA) depletion reagents to enable a comprehensive view of the transcriptome through superior removal of human, mouse, and rat rRNA. Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related prioduct, the Collibri Library Quantification Kit is recommended for qPCR-based quantifications of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

3500 Dx Series Sequencing Standard, BigDye™ Terminator v1.1 Applied Biosystems™

This BigDye® Terminator v1.1 sequencing standard consists of four tubes, each of which contains DNA of a known 1,200 base pair sequence prepared with BigDye® Terminator v1.1. Sufficient DNA is included to perform one spectral calibration/performance check and/or one control sequencing run on an Applied Biosystems® 3500 Dx Series genetic analyzer. The DNA preparation has been lyophilized to maximize stability.

Ion AmpliSeq™ Comprehensive Cancer Panel Ion Torrent™

The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. View the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (PDF).

The Ion AmpliSeq™ technology allows ultra-high multiplex PCR combined with targeted sequencing library construction in just 16 hours for over 400 genes. Utilizing only 10 ng of input DNA per primer pool for a total of 40 ng of input DNA, analysis of restricted samples like FFPE samples is possible. The Ion AmpliSeq™ Comprehensive Cancer Panel is optimized for library construction with the Ion AmpliSeq™ Library Kit 2.0. Libraries are then ready for template preparation on the Ion OneTouch™ System and sequencing on the Ion PGM™ Sequencer. The Ion AmpliSeq™ Comprehensive Cancer Panel is not compatible with Ion Ampliseq™ 2.0 Beta Kits.

Features and Benefits:

• Broad survey of 409 key genes in a simple PCR reaction, no additional capital equipment required
• Unmatched plexy of 16,000 primer pairs in only four pools with Ion AmpliSeq™ technology
• Low DNA input of only 40 ng DNA and short amplicons enable FFPE samples and needle biopsies
• Start comprehensive genomic studies quickly with pre-designed primer pools
• Simplify analysis and annotation of variants with Ion Reporter™ software

Comprehensive Gene Content Including Genes from Hotspot Panel
Ion Torrent has designed the most extensive cancer panel available for sequencing with Ion PGM™ and, soon, Ion Proton™ Sequencers. Working with key cancer researchers and reviewing literature and databases, the Ion AmpliSeq™ Comprehensive Cancer Panel was designed to target all exons of key tumor suppressor genes and oncogenes most frequently cited and most frequently mutated. Strategically designed to interrogate CDS and splice variants across multiple gene families simultaneously, pathway-based gene selection profiles mutational spectrum in cancer driver genes and drug targets, along with signaling cascades, apoptosis, DNA repair, transcription regulators, inflammatory response, and growth factor genes in a single assay. Additionally, all genes from the focused Ion AmpliSeq™ Cancer Panel are included in the Ion AmpliSeq™ Comprehensive Cancer Panel providing corresponding complementary data.

Unmatched Level of Multiplex PCR
The Ion AmpliSeq™ Comprehensive Cancer Panel is comprised of four primer pools totaling almost 16,000 primer pairs covering 409 genes-that's approximately 4,000 primer pairs in each pool! Used in conjunction with the Ion AmpliSeq™ Library Kit 2.0, the Ion AmpliSeq™ Comprehensive Cancer Panel maintains high uniformity and specificity at this high plexy, advancing multiplex PCR to new levels.

Over 400 Genes, Only 40 ng DNA Required
While other target selection methods typically require micrograms of DNA input, the Ion AmpliSeq™ Comprehensive Cancer Panel uses only 40 ng DNA. This breakthrough in low DNA input requirement enables the interrogation of restricted samples like formalin-fixed, paraffin-embedded samples (FFPE) and fine needle aspiration biopsies (FNAB). Additionally, shorter amplicon design (average 155 bp) allows for compatibility with degraded samples.

Simplified Target Selection Without Primer Design
With pre-designed primers in the Ion AmpliSeq™ Comprehensive Cancer Panel, researchers can bypass gene selection, primer design, and optimization, and advance straight to generating results for a broad survey of hundreds of genes in key research samples. To further focus your cancer research, you may choose to conduct in-depth somatic variant analysis on a subset of genes or specific cancer subtype. Using Ion AmpliSeq™ Designer, researchers can simply add or subtract genes from this panel to create your own custom panel. The flexibility and scalability of Ion AmpliSeq™ Solutions spans across the Ion AmpliSeq™ Ready-to-use Panels and Ion AmpliSeq™ Custom Panels.

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.
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