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AmpFLSTR™ Yfiler™ Direct Kit Applied Biosystems™

The AmpFLSTR Yfiler Direct Kit enables forensic laboratories to perform high-quality Y-STR analysis of single-source reference samples using direct PCR amplification. This kit amplifies the same 17 Y-STR loci as the AmpFLSTR Yfiler Kit and is designed to generate accurate results with faster thermocycling conditions than the Yfiler Kit. Blood or buccal samples on treated paper (FTA Card or NUCLEIC-CARD System) can be punched into PCR plates or tubes and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector and other untreated substrates can be processed without additional workflow steps using the Applied Biosystems Prep-n-Go Buffer.

Key Features:

• Supports direct PCR amplification (no DNA extraction or quantification required) of single source reference samples on treated and untreated paper
• Provides maximum concordance with the Yfiler kit
• Designed to deliver faster cycling times through the introduction of a new fast-capable enzyme (~2.5 hours)

In addition, the Yfiler Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Yfiler kit, which:

• Facilitates comparison with casework data
• Streamlines validation and implementation

Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR STR Kits, instrumentation, software, and consumables.

The AmpFLSTR Yfiler Direct Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification.

The Yfiler Direct kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

IMPORTANT NOTICE:
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

3130xl/3100 Genetic Analyzer 16-Capillary Array, 36 cm Applied Biosystems™

This is a 36 cm 16-capillary array (internally uncoated) for the 3130xl and 3100 Genetic Analyzer. Use for fragment analysis and rapid sequencing applications. Capillary dimensions are 47 cm x 50 µm i.d. Usage per array is 100 runs (with use of Applied Biosystems reagents).

BigDye XTerminator™ Purification Kit Applied Biosystems™

The BigDye XTerminator® Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye® terminators and salts. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.

• Eliminate liquid transfer – Use a single plate.
• Obtain complete dye blob removal.
• Stabilize the samples before analysis.
• Improve your sequencing workflow.
• Perform manual or automated protocols.
• Get rapid, reliable, and reproducible results.

Simple Purification Process
Traditional purification methods, such as ethanol precipitation, require the addition of multiple reagents along with decanting and centrifuging steps. The BigDye XTerminator® Purification Kit requires the addition of only two reagents which can be added sequentially or premixed:

• XTerminator™ Solution – Scavenges unincorporated dye terminators and free salts from the post-sequencing reaction.
• SAM™ Solution – Enhances the performance of the XTerminator™ Solution and stabilizes the post-purification reactions.

The protocol is very simple (see figure 1):
1. Perform cycle sequencing with BigDye® terminators.
2. After cycle sequencing, centrifuge the reaction plate briefly, and then pipette the correct volume of SAM™ Solution into each plate well.
3. Pipette the correct volume of XTerminator™ Solution into each well:
4. Vortex the XTerminator™ Solution bulk container briefly.
5. Using a wide-bore pipette tip (recommended wide-bore tips), pipette the correct volume of liquid into each plate well
6. Seal the plate using a heat seal or Clear Adhesive Film (P⁄N 4306311). Vortex for 30 minutes (recommended 96-well microplate vortexers and 384-well microplage vortexers), then centrifuge the reaction plate briefly.
7. Place the reaction plate in the Applied Biosystems DNA analyzer. Select run module and run plate.

Excellent Long Fragment Recovery
You won’t sacrifice sequence length when you use the BigDye XTerminator® Purification Kit. Figure 2 shows data from the DNA Technology Unit, Plant Biotechnology Institute (NRC-PBI) Saskatoon, Canada. The Q20 base read=1044 bp.

Improved Short Fragment Recovery
Use the BigDye XTerminator® Purification Kit to obtain higher quality data. Figure 3 shows a comparison between the BigDye XTerminator® Purification Kit and ethanol precipitation-based purification performed at the DNA Technology Unit, Plant Biotechnology Institute (NRC-PBI) Saskatoon, Canada. The BigDye XTerminator® Purification Kit results in superior short fragment recovery. Ethanol purification produces sequence data marred by dye blobs.

Increased Stability
Sealed reaction plates are stable for 48 hours at room temperature or 10 days at 4°C You can re-run the same sample in case of a power interruption, computer malfunction, etc. without having to repeat the experiment. This increases your flexibility when loading a reaction plate on a CE instrument.

Run Modules Available
Applied Biosystems provides download-able run modules for use with the BigDye XTerminator® Purification Kit and Data Collection Software. These modules are designed for the 3100, 3100–Avant (with Data Collection v2.0 or above), 3130⁄3130xl, and 3730⁄3730xl Analyzers. The software modules for the BigDye XTerminator® Purification Kit are compatible with the Windows® 2000 and Windows® XP operating systems and are available on our software download page:

http:⁄⁄www.appliedbiosystems.com⁄absite⁄us⁄en⁄home⁄support⁄software⁄dna-sequencing⁄bigdye-xterm-purif-kit.html

For Research Use Only. Not for use in diagnostics procedures.

Ion Plus Fragment Library Kit for AB Library Builder™ System Ion Torrent™

The Ion Plus Fragment Library Kit for AB Library Builder™ System provides a rapid, flexible, and high-throughput solution for library construction upstream of semiconductor sequencing when starting with fragmented DNA. This ready-to-use reagent cartridge simplifies next-generation sequencing by enabling automated DNA fragment library preparation for the Personal Genome Machine® and soon the Proton™ sequencing systems.

This kit contains sample preparation reagents for library construction of 13 DNA libraries for semiconductor sequencing. Use of this reagent cartridge requires additional standard or barcoded library adapters (sold separately).

This library kit offers:

Simplicity and convenience of automated library preparation upstream of semiconductor sequencing
Rapid and flexible workflow automating library creation and size selection in as little as 2 hours starting with pre-fragmented DNA
Lower input requirement providing the ability to make amplification-free libraries from as little as 50 ng starting material
Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

With greatly reduced hands-on time, the Ion Plus Fragment Library Kit for AB Library Builder™ System automates preparation of up to 13 standard, paired-end, or barcode fragment libraries in about 2 hours per run, starting with small amplicons or pre-sheared gDNA samples. Size selection of the libraries is performed automatically without the expense or effort of gel-based methods. Alternatively, libraries can be manually size-selected by conventional methods after the automated run.

Starting with 50–1000 ng of input DNA, the kit yields significantly higher amounts of library than current manual methods, eliminating the need for PCR amplification (i.e. 'amplification-free' libraries) for most sequencing needs.

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With the ability to prepare up to 13 libraries in a little over 2 hours, the Ion Plus Fragment Library Kit for AB Library Builder™ System is a key step towards automation of the Ion sequencing workflow, making comprehensive analyses of your sample(s) of interest more convenient than ever before. This modular reagent system allows for the construction of a variety of fragment libraries (i.e., standard and barcoded) depending on the library adapters used (library adapters sold separately).

Ion Torrent Sequencing Made Simpler, Faster, and More Affordable
The Ion Plus Fragment Library Kit for AB Library Builder™ System automates library creation and size selection, eliminating the need for costly time-consuming gel-based size selection, and requires as little as 50 ng of sample, accelerating your path to semiconductor sequencing. This automated library preparation solution provides fast and scalable workflows for sequencing projects requiring low- to high-throughput processing capabilities.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Collibri™ PS DNA Library Prep Kit for Illumina Systems, with UD indexes (Set C, 49-72) Invitrogen™

The Invitrogen Collibri PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PS DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success
• Includes all necessary components in a single kit
• Offers consistent, even coverage of variable GC regions from inputs ranging from 1 to 1,000 ng
• Offers improved coverage of challenging genomic regions compared to already available library prep technologies
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PS DNA Library Prep Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PS DNA Library Prep Kit consistently offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from a wide range of inputs (1 ng to 1 µg) of physically sheared double-stranded DNA (see figure below). Additionally, a PCR-free version is offered for inputs starting at 500 ng DNA. The entire library preparation protocol takes about 3.5 hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Reservoir Septa Applied Biosystems™

This is a Reservoir Septa for ABI PRISM™ 3100 Genetic Analyzer and ABI PRISM™ 3100-Avant™ Genetic Analyzer.

For Research Use Only. Not for use in diagnostics procedures.

Ion Xpress™ Plus Fragment Library Kit for AB Library Builder™ System Ion Torrent™

The Ion Xpress™ Plus Fragment Library Kit for AB Library Builder™ System provides a rapid, flexible, and high-throughput solution for library construction upstream of the semiconductor sequencing workflow. This ready-to-use reagent cartridge simplifies next-generation sequencing by enabling automated DNA fragment library preparation for the Personal Genome Machine® and soon the Proton™ sequencing systems.

This kit contains sample preparation reagents for enzymatic shearing and library construction of 13 DNA libraries for semiconductor sequencing. Use of this reagent cartridge requires the addition of library adapters (sold separately).

This library kit offers:

Simplicity and convenience of automated library preparation upstream of semiconductor sequencing
Superior coverage uniformity for diverse sample types using our proprietary Ion Shear™ Plus chemistry
Lower input requirement providing the ability to make amplification-free libraries from as little as 50 ng of starting DNA
Rapid and flexible workflow producing Ion fragment libraries containing user-selectable and automatically size-selected insert sizes of 100–400 bp in as little as 2 hours
Significant cost savings by removing the need for ancillary shearing devices and size-selection gels
Scalable methodology to meet high-throughput sequencing requirements

With its plug-n-play modular reagent design, this revolutionary kit automates all steps of library creation, including both DNA fragmentation and library size selection, in as little as 2 hours with limited set-up time and minimal subsequent user-intervention. DNA fragmentation is automated with enhanced, proprietary Ion Shear™ DNA fragmentation chemistry to achieve user-selectable inserts of 100–400 bp in length. Size selection of the libraries is performed automatically without the expense or effort of gel-based methods. Alternatively, libraries can be manually size-selected using conventional methods after the automated run. Starting with 50–1000 ng of input DNA, the kit yields significantly higher amounts of library than current manual methods, eliminating the need for PCR amplification (i.e., 'amplification-free' libraries) for most sequencing needs.

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With the ability to prepare up to 13 libraries in a little over 2 hours, the Ion Xpress™ Plus Fragment Library Kit for AB Library Builder™ System is a key step towards automation of the Ion sequencing workflow, making comprehensive analyses of your sample(s) of interest more convenient than ever before. Furthermore, Ion Xpress™ Plus fragment libraries generated using the AB Library Builder™ system exhibit the superb coverage uniformity typically seen with proprietary Ion Shear™ DNA fragmentation chemistry and higher recovery rates than other library construction methodologies. So you can be confident of generating the best data in the shortest time, without the hassle or expense of manual shearing and size selection.

Ion Torrent Sequencing Made Simpler, Faster, and More Affordable
Ion Shear™ Plus DNA fragmentation technology eliminates the need for costly physical shearing devices for most applications and requires as little as 50 ng of sample, accelerating your path to semiconductor sequencing. This flexible technology also provides users the ability to adjust fragmentation and size-selection sizes to accommodate a range of sequencing read lengths depending on their project requirements. This automated library preparation solution provides fast and scalable workflows for sequencing projects requiring low- to high-throughput processing capabilities.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Anode Buffer Reservoir Jar Applied Biosystems™

This Buffer Reservoir is for use with the 3100-Avant™ Genetic Analyzer. It attaches to the lower polymer block, holding the anodic buffer.

For Research Use Only. Not for use in diagnostics procedures.

Anode Buffer Container (ABC) for 3500 Series Systems for Protein Quality Analysis Applied Biosystems™

The Anode Buffer Container (ABC) contains 1X running buffer to support protein quality analysis applications on the Applied Biosystems™ 3500 Series genetic analyzers for protein quality analysis. The ABC is ready-to-use and disposable with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat-sealed with a plastic film, which is removed prior to installation on the instrument. Each package includes four containers.

Ion 510™ & Ion 520™ & Ion 530™ Food Protection Kit – Chef (2 sequencing runs per initialization) Thermo Scientific™

Perform robust and automated template preparation and sequencing of up to 400 base-read libraries using the Ion 510™ & Ion 520™ & Ion 530™ Food Protection Kit – Chef (2 sequencing runs per initialization)  as a part of Thermo Scientific™ Next Generation Sequencing (NGS) Food Authenticity Workflow.

The Thermo Scientific™ NGS Food Authenticity Workflow allows identification of the meat, plant and fish species present in even the most challenging sample types; protecting your business from food fraud by verifying the authenticity of samples for improved traceability and food safety.

MicroSEQ™ D2 rDNA Fungal Sequencing Kit Applied Biosystems™

This kit is the sequencing component of the MicroSEQ® Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi. It includes the primers needed to sequence the PCR products generated using the PCR component. A protocol and Quick Reference Card are not included.
• Sequence-based measurement ensures you get reproducible results in less than 24 hours.
• Growth requirement-independent measurement enables you to identify fungi using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ® ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training; same work flow as the MicroSEQ® Full Gene 16S rDNA Bacterial Identification Kit.

Specific and Easy to Use
Based on phylogenetic analysis of the D2 region of large-subunit ribosomal RNA gene sequences, this easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1,000 validated type species.

The MicroSEQ® ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems® MicroSEQ® System combines the advantages of MicroSEQ® ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ® bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.

Collibri™ PS DNA Library Prep Kit for Illumina Systems, with UD indexes(Set D, 73-96) Invitrogen™

The Invitrogen Collibri PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PS DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success
• Includes all necessary components in a single kit
• Offers consistent, even coverage of variable GC regions from inputs ranging from 1 to 1,000 ng
• Offers improved coverage of challenging genomic regions compared to already available library prep technologies
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PS DNA Library Prep Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PS DNA Library Prep Kit consistently offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from a wide range of inputs (1 ng to 1 µg) of physically sheared double-stranded DNA (see figure below). Additionally, a PCR-free version is offered for inputs starting at 500 ng DNA. The entire library preparation protocol takes about 3.5 hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

MicroSEQ™ ID Microbial Identification Software v3.0 Lite Bundle Applied Biosystems™

MicroSEQ® ID Microbial Identification Software allows you to easily identify and classify unidentified bacterial or fungal sequences by comparing them to a validated microbial library. The Lite version of the software is for use on a stand-alone PC (not connected to the genetic analyzer) for simple analyses. This bundle includes the bacterial and fungal sequence libraries.

• A validated library of over 2300 microbial sequences help ensure you get the answers you need
• Automatically identify unknown specimens
• Run plates on Applied Biosystems® 3500 Series Genetic Analyzers directly via the MicroSEQ® ID Software
• MicroSEQ® system is a complete integrated solution, including security, audit, and e-signature features
• Qualification and validation support

Confidently Identify Microbes
The MicroSEQ® system comprises bacterial libraries of 500 bp 16S rDNA sequences and over 2300 validated entries--including extensive coverage of gram-negative nonfermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. A fungal library containing more than 1100 entries is also included. To help ensure accurate identification of the species of your sequences, we rigorously validate all library entries. And unlike other widely available databases, MicroSEQ® libraries contain only correctly identified organisms and quality-controlled sequences derived from type strains of preeminent culture collections. The libraries also support sequence polymorphisms, and include bacterial Category A biothreat agents.

Automatically Identify Unknown Specimens
Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The new Auto-ID feature in MicroSEQ® ID Software v3.0 uses a set of editable parameters to automatically identify unknown specimens. The results table is displayed in the report, and an editable comments field allows additional information or manual identifications to be entered into the same table.

Run Plates on 3500/3500xL Instruments Directly via the MicroSEQ® ID Software
Applied Biosystems® 3500 Series Genetic Analyzer users are now able to run plates on these instruments directly via the MicroSEQ® ID Software v3.0 user interface, without needing Autoanalysis Manager. A single software wizard enables you to set up projects, specimens, and plates; adjust the plate layout via drag and drop; start and monitor the run; and review results.

The MicroSEQ® System: A Complete, Integrated Solution
Streamlining every step in microbial identification, the Applied Biosystems® MicroSEQ® system combines the advantages of MicroSEQ® ID Software with easy to use PrepMan® Ultra sample preparation reagents and protocols, MicroSEQ® bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. The MicroSEQ® ID Software also includes security, audit, and electronic signature features enabling the software to smoothly integrate into your workflow.

Qualification and Validation Support
To help ensure seamless integration into your laboratory, from installation to routine operation, we offer comprehensive installation and operational qualification (IQ/OQ) and validation support services.

For Research Use Only. Not for use in diagnostics procedures.

Prep-n-Go™ Buffer (for buccal swabs) Applied Biosystems™

Prep-n-Go™ Buffer (for buccal swabs) is a best-in-class buffer designed to enable high-quality direct PCR amplification of single source samples collected on buccal swabs and amplified using the AmpFLSTR® Identifiler® Direct PCR Amplification Kit or other Applied Biosystems® STR direct chemistries. This product expands direct PCR amplification capabilities to laboratories that use a wide variety of substrates, and is readily adaptable to both manual and automated workflows. No purification, extraction, or quantification is required, thus enabling laboratories to significantly expand their throughput capabilities, while also maximizing sample integrity and data quality.

Key Features:

• Designed to provide consistent, interpretable peaks above the detection threshold, and high quality, well-balanced STR profiles
• Helps simplify automation requirements and expand throughput capabilities
• Helps maximize sample integrity and data quality
• Developed according to stringent performance standards to enable higher data reproducibility, reduced artifacts, and increased success rates with Applied Biosystems® STR direct amplification solutions

Qubit™ Food Protection Starter Pack Thermo Scientific™

Quickly and accurately quantify extracted DNA using the highly sensitive Thermo Scientific™ Qubit™ The Qubit Food Protection Starter Pack is for use with the Thermo Scientific™ NGS Food Authenticity Workflow.

The Thermo Scientific NGS Food Authenticity Workflow allows identification of the meat, plant or fish species present in even the most challenging sample types, protecting your business from food fraud.

The Qubit Food Protection Starter Pack provides everything you need for 500 test reactions―a Qubit 4 Fluorometer, Qubit Assay Tubes and Qubit dsDNA BR Assay Kit.

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