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Ion AmpliSeq™ Direct FFPE DNA Kit Ion Torrent™

The Ion AmpliSeq™ Direct FFPE DNA Kit enables the preparation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissues for library construction using the Ion AmpliSeq protocol, without the need for deparaffinization or DNA purification. This kit is suitable for FFPE tissue sections up to 10 µm thick.

Features of the Ion AmpliSeq Direct FFPE DNA Kit include:
• Simple one-tube 2-step protocol that minimizes sample loss
• Efficient 30-minute workflow with only 10 minutes of hands-on time
• Ideal for FFPE DNA samples with limited DNA amount
• No deparaffinization of FFPE sections required
• No column or bead-based DNA purification required
• Ion AmpliSeq library preparation with as little as 1 ng input DNA
• Uracil-D-glycosylase treatment to remove deaminated cytosines is an optional step

Extraction of DNA from samples with limited DNA amount
Archived tissue samples can provide clinically relevant information, but it can be difficult to isolate the small amount of nucleic acid they contain. Deparaffinization of FFPE tissue sections and purification of the DNA in these samples can be a complicated, multi-step procedure requiring considerable hands-on time, and the associated sample-loss makes it an unsuitable approach for processing FFPE DNA samples with limited DNA amounts.

A simpler and faster method
The Ion AmpliSeq Direct FFPE DNA Kit provides a user-friendly protocol that eliminates the need for deparaffinization of FFPE tissue sections and purification of the DNA. From an FFPE section mounted on a glass slide, the area of interest is removed using Transfer Solution and pipetted into a PCR tube or 96-well plate. After the addition of Direct Reagent, the sample is incubated at 65°C for 15 minutes. The FFPE DNA is now ready for the Ion AmpliSeq™ Library Preparation protocol. The prepared FFPE DNA can be quantified using the Qubit™ HS DNA Quantitation Kit, if necessary. The simplicity of this protocol eliminates the need for complicated deparaffinization and DNA extraction procedures, and libraries can be prepared either manually or with the automated Ion Chef™ System.

Oncomine™ Lung cfDNA Assay Ion Torrent™

The Oncomine™ Lung cfDNA Assay is part of a complete research solution to detect lung tumor-derived DNA (ctDNA) in cfDNA. It provides the reagents for library construction and a single pool of multiplex PCR primers for preparation of amplicon libraries from cell-free DNA (cfDNA) obtained from the plasma fraction of a single tube of whole blood. The assay enables researchers to analyze single nucleotide variants and short indels that are frequently mutated in non-small cell lung cancer (NSCLC). Eleven genes and >150 hotspots are covered (Genes: ALK, BRAF, EGFR, ERBB2, KRAS, MAP2K1, MET, NRAS, PIK3CA, ROS1, and TP53). Through the use of proprietary sequencing technology, a limit of detection (LOD) down to 0.1% can be achieved.

Note: Target and hotspot BED files and User Guide document for cfDNA Variant Analysis with Torrent Suite Software 5.2 can be found below under 'Documents'.

• Optimized amplicon design for short cfDNA (~170 bp)
• Detection of both single nucleotide variants (SNVs) and short indels down to 0.1% LOD
• Optimized targeted assay design allows highly multiplexed next-generation sequencing (NGS), reducing sequencing costs
• Very high sensitivity and specificity, enabling extremely low levels of detection with minimal false positives
• Efficient workflow, from a single tube of blood to results in just 2 days

Why are pathologists and oncologists interested in liquid biopsy clinical cancer research? Liquid biopsies offer several advantages over conventional solid tumor samples:
• Less invasive to obtain, enabling tumor content to be sampled multiple times
• Lower cost compared to traditional tissue samples
• Faster turnaround time from sample to results
• Help capture more of the heterogeneity of the tumor
• May lead to improvements in standard of care in the future

The entire workflow (figure below) from isolation of cfDNA using the MagMAX™ Cell-Free DNA Isolation Kit to analysis of samples can be accomplished in just 2 days using the Ion Chef™ System and the Ion S5™ XL sequencing system.

Unlike other technologies with low LODs (1-5%), the Oncomine Lung cfDNA Assay has a flexible detection limit down to 0.1% or 1 mutant copy in a background of 1,000 wild-type copies. To achieve 0.1% LOD, 20 ng of input cfDNA is required. Lower amounts of cfDNA can be used, but the resulting LOD will be higher depending on the input amount.

Simplicity, speed, and scalability
The Oncomine Lung cfDNA Assay enables cancer genetic studies from as little as 1 ng of input cfDNA for targeted library construction. The cfDNA assay uses standard PCR equipment and two simple PCR reactions, one for sequencing and the second to amplify the library for high multiplex PCR-based target selection, which minimizes the need for extensive capital investment. Additionally, the Oncomine Lung cfDNA Assay is compatible with FFPE samples for possible concordance studies. Total time to targeted libraries is typically just 3.5 hours. Scalability and flexibility are achieved using the Tag Sequencing Barcode Set 1-24

Genexus™ Barcodes 1-96 AS Ion Torrent™

The Ion Torrent Genexus Barcodes 1-96 AS kit provides one each of Ion Torrent Genexus Barcodes 1-32 AS, 33-64 AS, and 65-96 AS. When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, it supports flexible, fully automated preparation and normalization of up to 96 Ion AmpliSeq libraries. Each plate is filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-96 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

ONC ION AS LIB KIT PLUS 24 RXN Ion Torrent™

This is a replicate of the existing AmpliSeq SKU 4488990 for revenue under the CS4 PL to be bundled with Oncomine tumor specfic panels.

Genexus™ Barcodes 1-32 AS Ion Torrent™

The Ion Torrent Genexus Barcodes 1-32 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 33-64 AS, 65-96 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-32 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Ion AmpliSeq™ Library Kit 2.0 Ion Torrent™

The Ion AmpliSeq™ Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ panels.

Scalable Multiplex PCR Reactions
The Ion AmpliSeq 2.0 technology enables scalable multiplex PCR reactions from 12- to 24,000-plex in a single well using just 10 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Ion AmpliSeq™ Custom Primer Pools are designed via the Ion AmpliSeq™ Designer Tool, available at www.ampliseq.com.

Barcoded Library Preparation
The Ion AmpliSeq Library Kit 2.0 includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1-96 kits or IonCode™ Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and Flexible Protocol
The Ion AmpliSeq Library Kit 2.0 uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The library preparation uses very small quantities of starting material per PCR. The resulting DNA libraries are ready for downstream template preparation using the automated Ion Chef™ System (which also provides the option of automated library preparation using the Ion AmpliSeq™ Kit for Chef DL8) or the Ion OneTouch™ 2 System followed by sequencing. The intuitive Torrent Suite™ Software and optional Ion Reporter™ Software enables automation of data analysis.

3500 Genetic Analyzer for Protein Quality Analysis Applied Biosystems™

The 3500 Genetic Analyzer for Protein Quality Analysis is a capillary electrophoresis instrument with an 8-capillary array specifically designed to support the demanding performance needs of biopharmaceutical screening and quality control environments, while retaining the unsurpassed application versatility appreciated by scientists working in analytical and process development environments. The instrument enables parallel analysis of 8 samples without compromising on the run time per sample, thereby enabling high throughput analysis with high quality data.

This system includes an 8-capillary instrument and a Dell Workstation and monitor, as well as reagent kits for system qualification. For a system that includes GlycanAssure™ Acquisition and Analysis Software, please see Cat. No. A30886.

Features include:
• An 8-capillary system that can easily be upgraded to a 24-capillary system when you need higher throughput
• Single-line, 505 nm, solid-state, long-life laser that utilizes a standard power supply and requires no heat removal
• Radio frequency identification (RFID) technology that tracks key consumables data and records administrative information
• Advanced multiplexing capabilities for glycan analysis with up to six unique dyes
• Unrivaled application flexibility—one array and one polymer are used for most applications
• Simple setup, operation, and maintenance

Request more information >

Accurate, precise, and reliable data quality
The 3500 Series systems significantly improve signal uniformity from instrument-to-instrument, run-to-run, and capillary array-to-capillary array. The systems provide accurate, reproducible, and precise relative quantitation of glycan levels.

Ready-to-use consumables with RFID tags
The 3500 Series systems introduce ready-to-use consumables with pre-packaged polymer pouches, cathode and anode buffer containers, and easy-to-install capillary arrays. Each of these consumables is integrated with RFID tags that enable viewing, tracking, and reporting of critical information about reagents and consumables, including usage, lot number, part number, expiry date, and on-instrument lifetime within the GlycanAssure data acquisition software.

Additional products
The following products are required for use with this system:
A31278: Anode Buffer Container (ABC) for 3500 Series for Protein Quality Analysis
A31279: Cathode Buffer Container (CBC) for 3500 Series for Protein Quality Analysis
A30936: POP-7™ for Protein Quality Analysis (384 samples)

ONC ION AS LIB KIT PLUS 96 RXN Ion Torrent™

This is a replicate of the existing AmpliSeq SKU A35907 for revenue under the CS4 PL to be bundled with Oncomine tumor specfic panels.

SGS™ All Species ID Food DNA Analyser Kits Thermo Scientific™

Identify the species present in even the most challenging sample types with the Thermo Scientific™ Next Generation Sequencing (NGS) Food Authenticity Workflow incorporating SGS™ All Species ID Food DNA Analyser Kits.

Protect your business from food fraud by verifying the meat, plant or fish species in your food samples for improved traceability and food safety.

As part of a complete, simple Next Generation Sequencing workflow, these kits provide a list of meat, plant or fish species present in all types of food and feed products including complex and highly processed sample types.

AmpFLSTR™ NGM SElect™ PCR Amplification Kit Applied Biosystems™

The NGM SElect Kit is a highly robust, single amplification Short Tandem Repeat (STR) multiplex kit developed for human identification laboratories wishing to utilize the expanded European Standard Set of Loci along with the highly polymorphic SE33 locus. The NGM SElect Kit combines the latest advances in primer synthesis, buffer enhancement and thermal cycling optimization. This enables the kit to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features of the NGM SElect Kit:

• Exceptional data quality and discrimination capabilities support international data sharing
• Enables significantly enhanced performance on inhibited and degraded samples
• Enables greater sensitivity
• Maximizes concordance with previously typed samples
• Facilitates confident interpretation, promotes an optimized workflow and streamlines the implementation process

Data Quality Worth Sharing
The NGM SElect Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP), and includes the expanded European Standard Set of Loci (ESSL).

The NGM SElect kit simultaneously amplifies the same 16 loci included in the NGM kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel (Figure 1).

Configured for Success
Utilizing our expertise in 5-dye and mobility modifier technology, 17 loci are accommodated in a single amplification while maintaining optimum spacing (Figure 2). This permits unambiguous allele designation. All amplicons fall below 450 base pairs (bp) to maximize performance across a wide range of forensic samples. The majority of loci are concentrated below 200 bp, enabling recovery of a greater number of alleles from challenging degraded samples.

Powerful Discrimination
The combination of loci in the NGM SElect kit generates the highest power of discrimination of any AmpFLSTR kit (Table 1). This helps minimize adventitious matches when comparing large data sets (e.g. for cross border data exchange). The NGM SElect kit also offers utility for other applications requiring higher levels of discrimination, including:
• Complex kinship analysis
• Disaster victim identification
• Missing person investigations

Permits Significantly Enhanced Performance on Inhibited and Degraded Samples
Leveraging the recent advances in PCR amplification technology pioneered with the NGM kit, the NGM SElect kit enables recovery of data from severely inhibited and degraded samples. For example, the NGM SElect kit is able to extract full profiles even from samples containing high concentrations of PCR inhibitors such as humic acid (Figure 3).

Performs with Greater Sensitivity
The NGM SElect Kit benefits from the same sensitivity enhancements as the NGM Kit, resulting in superior allele recovery at lower input levels (Figure 4). This can also enhance detection of the minor contributor in a mixed sample. The standard 29 cycle, 1 ng DNA input protocol enables maximum profile balance, and a validated 30 cycle protocol is also available. The higher cycle number leads to a corresponding increase in signal, increasing the possibility of allele detection from lower input DNA amounts. The use of 30 cycles also results in offscale data at an input of 1 ng (Figure 4); therefore, laboratories wishing to adopt this protocol should determine the optimum input DNA appropriate to their workflow through internal validation studies.

Maximizes Concordance
The NGM SElect Kit maintains identical primer sequences for the core STR loci common to the SGM Plus, Identifiler and SEfiler Plus kits, thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements

The primers for the SE33 locus were redesigned from those used in the SEfiler Plus kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus kit as much as possible.

Facilitates Confident Data Interpretation
The NGM SElect kit benefits from improvements in primer synthesis techniques resulting in significantly cleaner baselines than previous AmpFLSTR kits (Figure 5). The lack of dye related artifacts, even at 30 cycles, improves the ability of the analyst to interpret data, particularly for low level samples. Confident data interpretation is further enhanced by other improvements in profile quality, including:
• Enhanced sensitivity and robustness
• Maximized heterozygote and intracolor balance
• Optimum dye set utilization

These features also help facilitate streamlined processing of single source samples by expert system software such as GeneMapper ID-X.

Easy to Implement, Easy to Use
Every aspect of the NGM SElect kit has been designed with ease of implementation and use in mind. This is a single kit solution that delivers high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs.

The kit workflow is very similar to other AmpFLSTR kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened by approximately one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Genetic Analyzer Sample Tubes, 0.5 mL Applied Biosystems™

These are sample tubes for use in the 48-sample tray of the 310 Genetic Analyzer.

Use with: 1.0 mL Glass Syringe, 310 Genetic Analyzer Capillary (47 cm, 36 cm wtr), 310 Running Buffer (10X), BigDye® Terminator v1.1 Cycle Sequencing Kit, GeneScan™ 500 ROX™ Size Standard, Hi-Di™ Formamide, MicroAmp® Reaction Tube with Cap (0.2 ml), POP-4™ Polymer for the 310 Genetic Analyzer, POP-6™ Polymer for the 310 Genetic Analyzer, Septa for 0.5 ml Sample Tubes

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Genexus™ Barcodes 33-64 AS Ion Torrent™

The Ion Torrent Genexus Barcodes 33-64 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 1-32 AS, 65-96 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 33-64 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Ion Sphere™ Quality Control Kit Ion Torrent™

The Ion Sphere Quality Control Kit furnishes a complete set of reagents for performing templated bead quality control using the Qubit 3.0 Fluorometer. This comprehensive kit contains calibration controls, buffers, and a probe mix for quality assessment of pre-enriched and post-enriched template-positive Ion Spheres. The powerful combination of the Ion Sphere Quality Control Kit and the Qubit 3.0 Fluorometer gives you greater experimental control and significant time and cost savings. By providing the confidence to generate high quality sequencing data with every run, this quality control process is ideal for those who are new to next-generation sequencing.

The Ion Sphere Quality Control Kit contains reagents for 20 quality control measurements. This kit is compatible with Qubit 2.0 and 3.0 fluorometers.

The Ion Sphere Quality Control Kit Gives You:
• Higher quality sequencing results through robust templated bead quality assessment
• A significantly quicker and more economical method for evaluating templated bead quality
• An increased level of experimental control and confidence

Rapidly Increase Your Proficiency in Semiconductor Sequencing
Quality control is essential for experimental success with new technologies. The Sphere Quality Control kit combined with the Qubit 3.0 Fluorometer offers a low cost, rapid, easy-to-use quality assessment solution, accelerating your proficiency in semiconductor sequencing. Don’t suffer by spending hours troubleshooting with “black-box" sequencers. Get high quality sequencing data the first time.

Enjoy Higher Confidence in Your Results
The Ion Sphere Quality Control Kit with the Qubit 3.0 Fluorometer brings greater experimental control to your science. For FFPE, LCM, or single cell applications where input material may be compromised, this robust assay determines sample quality prior to enrichment and sequencing, saving you time and money.

Benefit from a Quality Control Kit Designed for the Highly Accurate Qubit 3.0 Fluorometer
With state-of-the-art optical components, the capability for sophisticated data analysis, and an intuitive user interface, the Qubit 3.0 Fluorometer platform provides highly accurate and specific measurements not only of templated Ion Sphere Particles, but also of DNA, RNA, or protein. Through the use of dye-based quantitation, the Qubit 3.0 Fluorometer enhances your productivity by letting you avoid costly repetition of experiments.

ONC ION AS LIB KIT PLUS 384 RXN Ion Torrent™

This is a replicate of the existing AmpliSeq SKU A38875 for revenue under the CS4 PL to be bundled with Oncomine tumor specfic panels.

Oncomine™ BCR IGH SR Assay, RNA Ion Torrent™

The Oncomine BCR IGH-SR Assay, RNA, is a robust, targeted next-generation sequencing (NGS) assay designed for hematology-oncology and minimal residual disease (MRD) monitoring research, pharmacodynamics and biomarker analysis of immunotherapy, as well as infectious and autoimmune disease research. The assay kit includes a single pool of multiplex PCR primers, total RNA-to-cDNA synthesis kit and Ion AmpliSeq library reagents.

The assay accurately identifies and measures clonal expansion of B lymphocytes using total RNA from bone marrow, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh-frozen and formalin-fixed paraffin-embedded (FFPE) research samples. The assay identifies unique B-cell clones through targeting of the highly diverse complementarity-determining region 3 (CDR3) of the B-cell receptor IGH chain from mRNA template via AmpliSeq multiplex framework 3 and joining gene primers. The nucleotide sequence of the IGH CDR3 region serves as a natural barcode to enable clone tracking for MRD clinical research and measurements of B-cell clonal expansion and diversity for pharmacodynamics and vaccine biomarker research. IGH CDR3 region amino acid motifs may reveal signatures of B-cell responses to defined antigens for use as future markers of autoimmune or infectious disease. RNA input improves detection of changes in plasmablast and plasma cell populations following immune challenge or administration of immunomodulatory agents. For DNA samples, please see Cat. No. A45483.

Benefits of the Oncomine BCR IGH SR Assay, RNA, include:
• High sensitivity and exceptionally low limit of detection for rare clone detection and tracking in research samples via dual-barcode indexing and high template capture efficiency of AmpliSeq multiplex PCR
• Compatibility with an array of research sample types, including fresh-frozen and FFPE tissue, bone marrow, whole blood, PBLs, and PBMCs
• Assay primers design to amplify all variable and joining gene alleles in the gold-standard IMGT database
• Ability to generate large libraries with minimal input requirement, facilitating detection of rare clones at a frequency of 10-6 from a single library preparation
• Efficient workflow using the Ion Chef templating system and Ion GeneStudio S5 sequencing system, enabling library-to-results time within 48 hours
• Flexible input requirements ranging from 25 ng to 2 µg
• Streamlined and user-friendly informatics solution with automated clonotyping, clonal lineage analysis, reporting of key repertoire features, and multi-sample analysis capability to enable longitudinal tracking in research samples
• Ultra-low substitution error rate minimizing artifacts arising from sequencing errors, enabling highly accurate assessment of clonal heterogeneity in malignant B-cell research samples of interest

The Oncomine BCR IGH-SR Assay, RNA, uses multiplex PCR primers to generate 110-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements.

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

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