Shop All DNA Synthesis Reagents and Columns

Synthesis Column Filters Applied Biosystems™

These filters are intended for use with empty synthesis columns to allow users to pack columns manually.

For Research Use Only. Not for use in diagnostics procedures.

CPG (Controlled Pore Glass) Synthesis Column, 1.0 µmol, 1000 Angstrom Pore Size, dA(Bz) Applied Biosystems™

The 1.0 µmol dA(Bz) Controlled Pore Glass (CPG) columns are suitable for the synthesis of any length oligonucleotide. These columns deliver high-quality oligonucleotides.

• Proprietary design and manufacturing methods ensure consistency and high quality
• Each column is tested to verify accuracy and precision of fill, then pressure-tested to verify proper assembly and to guarantee each column is leak-free
• The 1,000 angstrom pore size allows synthesis of oligonucleotides of varying length

These columns are fully compatible with the Applied Biosystems 3400 instruments, ABI 391, ABI 392, ABI 394, and earlier instruments.

For Research Use Only. Not for use in diagnostics procedures.

CPG (Controlled Pore Glass) Synthesis Column, 0.2 µmol, 500 Angstrom Pore Size, dG (iBu) Applied Biosystems™

The 0.2 µmol dG(iBu) Controlled Pore Glass (CPG) columns are suitable for the synthesis of oligonucleotides<50 bases in length. These columns deliver high-quality oligonucleotides.

• Proprietary design and manufacturing methods ensure consistency and high quality
• Each column is tested to verify accuracy and precision of fill, then pressure-tested to verify proper assembly and to guarantee each column is leak-free
• The 500 angstrom pore size allows synthesis of oligonucleotides of up to 50 bases.

These columns are fully compatible with the Applied Biosystems 3400 instruments, ABI 391, ABI 392, ABI 394, and earlier instruments.

For Research Use Only. Not for use in diagnostics procedures.

Bulk CPG (Controlled Pore Glass), 500 Angstrom pore size, dA(Bz) Applied Biosystems™

Bulk dA(Bz) Controlled Pore Glass (CPG) is available for users to pack their own columns for synthesis of oligonucleotides <50 bases in length.

• Proprietary design and manufacturing methods ensure consistency and high quality
• The 500 angstrom pore size of the support allows synthesis of oligonucleotides of up to 50 bases
• Save time and money

For Research Use Only. Not for use in diagnostics procedures.

Iodine-Water-Pyridine-Tetrahydrofuran Oxidizer Solution (0.02 M) Applied Biosystems™

This Iodine⁄Water⁄Pyridine⁄Tetrahydrofuran (oxidizer) solution comes in a convenient large volume for ABI 394 Nucleic Acid synthesizers, resulting in extended hands-free DNA synthesis. With these larger bottles attached your synthesizer can produce up to 40 average primer-length oligonucleotides with no bottle changes, reducing attended operation time by 50%.

• Reagent concentrations are within our strict specifications, guaranteeing consistent quality.
• Our performance specification of at least 98% stepwise coupling efficiency is assured, increasing oligonucleotide synthesis yields of full-length product.
• Reagent kits are available for your convenience, and save you time and money.

Our high-performance reagents begin with raw materials that must conform to strict specifications. Once the reagents are manufactured and packaged, we perform extensive testing to assure quality. Reagent lifetime on the instrument is two weeks for tetrazole, and six weeks for all other reagents.

For Research Use Only. Not for use in diagnostics procedures.

Polystyrene Synthesis Column, ABI LV40™, dA(Bz) Applied Biosystems™

The dA(Bz) ABI LV40® columns employ the advantages of polystyrene and a low volume column design to deliver high-quality oligonucleotides, while saving time and money.

• Proprietary design and manufacturing methods ensure consistency and high quality.
• Each column is tested to verify accuracy and precision of fill, then pressure-tested to verify proper assembly and to guarantee each column is leak-free.
• Hydrophobic polystryrene columns sustain anhydrous environment to promote efficient coupling while minimizing nonspecific binding sites, yielding an efficient synthesis.
• The 1,000 angstrom pore size of the polystyrene support allows synthesis of oligonucleotides of varying length.

Save Time and Money
Compared to conventional columns, the ABI LV40® columns reduce cycle time by about 10% and the cost of synthesis by about 25%. These columns are fully compatible with the Applied Biosystems 3400 instruments, ABI 391, ABI 392, ABI 394, and earlier instruments.

For Research Use Only. Not for use in diagnostics procedures.

Anhydrous Acetonitrile Applied Biosystems™

This case contains 4 x 30 mL bottles of anhydrous acetonitrile.

This anhydrous acetonitrile (amidite diluent) comes in a convenient large volume for ABI 394 Nucleic Acid synthesizers, resulting in extended hands-free DNA synthesis. With these larger bottles attached your synthesizer can produce up to 40 average primer-length oligonucleotides with no bottle changes, reducing attended operation time by 50%.

• Reagent concentrations are within our strict specifications, guaranteeing consistent quality.
• Our performance specification of at least 98% stepwise coupling efficiency is assured, increasing oligonucleotide synthesis yields of full-length product.
• Reagent kits are available for your convenience, and save you time and money.

Our high-performance reagents begin with raw materials that must conform to strict specifications. Once the reagents are manufactured and packaged, we perform extensive testing to assure quality. Reagent lifetime on the instrument is two weeks for tetrazole, and six weeks for all other reagents.

For Research Use Only. Not for use in diagnostics procedures.

CPG (Controlled Pore Glass) Synthesis Column, 1.0 µmol, 1000 Angstrom Pore Size, dG(iBu) Applied Biosystems™

The 1.0 µmol dG(iBu) Controlled Pore Glass (CPG) columns are suitable for the synthesis of any length oligonucleotide. These columns deliver high-quality oligonucleotides.

• Proprietary design and manufacturing methods ensure consistency and high quality
• Each column is tested to verify accuracy and precision of fill, then pressure-tested to verify proper assembly and to guarantee each column is leak-free
• The 1,000 angstrom pore size allows synthesis of oligonucleotides of varying length

These columns are fully compatible with the Applied Biosystems 3400 instruments, ABI 391, ABI 392, ABI 394, and earlier instruments.

For Research Use Only. Not for use in diagnostics procedures.

Tetraethylthiuram Disulfide Acetonitrile (TETD) Sulfurizing Reagent, 450 mL Applied Biosystems™

This is Tetraethylthiuram Disulfide Acetonitrile (TETD) Sulfurizing Reagent (450 mL).

Tetraethylthiuram Disulfide Acetonitrile (TETD) Sulfurizing Reagent is used for making phosphorothiate backbone oligonucleotides. TETD comes in a convenient large volume for ABI 394 Nucleic Acid synthesizers, resulting in extended hands-free DNA synthesis. With these larger bottles attached your synthesizer can produce up to 40 average primer-length oligonucleotides with no bottle changes, reducing attended operation time by 50%.

• Reagent concentrations are within our strict specifications, guaranteeing consistent quality.
• Our performance specification of at least 98% stepwise coupling efficiency is assured, increasing oligonucleotide synthesis yields of full-length product.
• Reagent kits are available for your convenience, and save you time and money.

Our high-performance reagents begin with raw materials that must conform to strict specifications. Once the reagents are manufactured and packaged, we perform extensive testing to assure quality. Reagent lifetime on the instrument is two weeks for tetrazole, and six weeks for all other reagents.

For Research Use Only. Not for use in diagnostics procedures.

CPG (Controlled Pore Glass) Synthesis Column, 0.2 µmol, 500 Angstrom Pore Size, T Applied Biosystems™

The 0.2 µmol T Controlled Pore Glass (CPG) columns are suitable for the synthesis of oligonucleotides<50 bases in length. These columns deliver high-quality oligonucleotides.

• Proprietary design and manufacturing methods ensure consistency and high quality
• Each column is tested to verify accuracy and precision of fill, then pressure-tested to verify proper assembly and to guarantee each column is leak-free
• The 500 angstrom pore size allows synthesis of oligonucleotides of up to 50 bases.

These columns are fully compatible with the Applied Biosystems 3400 instruments, ABI 391, ABI 392, ABI 394, and earlier instruments.

For Research Use Only. Not for use in diagnostics procedures.

Tetrazole Acetonitrile Solution Applied Biosystems™

This Tetrazole⁄Acetonitrile solution comes in a convenient large volume for ABI 394 Nucleic Acid synthesizers, resulting in extended hands-free DNA synthesis. With these larger bottles attached your synthesizer can produce up to 40 average primer-length oligonucleotides with no bottle changes, reducing attended operation time by 50%.

• Reagent concentrations are within our strict specifications, guaranteeing consistent quality.
• Our performance specification of at least 98% stepwise coupling efficiency is assured, increasing oligonucleotide synthesis yields of full-length product.
• Reagent kits are available for your convenience, and save you time and money.

Our high-performance reagents begin with raw materials that must conform to strict specifications. Once the reagents are manufactured and packaged, we perform extensive testing to assure quality. Reagent lifetime on the instrument is two weeks for tetrazole, and six weeks for all other reagents.

For Research Use Only. Not for use in diagnostics procedures.

dAbz, 0.2 µmol ABI 3900 HT Columns Applied Biosystems™

The 0.2 µmol d(Abz) ABI 3900 HT columns are fully assembled, low-cost columns that produce high-quality oligonucleotides. These supports are filled with polystyrene, a material which has several advantages over the more conventional, controlled pore glass (CPG) filled columns.

• Proprietary design and manufacturing methods ensure consistency and high quality to give you optimal synthesis quality.
• Hydrophobic, polystyrene-filled columns sustain an anhydrous environment to promote efficient coupling while minimizing nonspecific binding sites, yielding an efficient synthesis.
• Polystyrene support allows synthesis of oligonucleotides of a variety of lengths, giving you flexibility in high-throughput oligonucleotide synthesis.

Designed for Performance
The greater hydrophobicity of polystyrene results in a higher yield of full-length product. Side reactions, such as extraneous chain growth, are minimized by the inert nature of this support surface.

For Research Use Only. Not for use in diagnostics procedures.

Bulk CPG (Controlled Pore Glass), 500 Angstrom Pore Size, dC(Bz) Applied Biosystems™

Bulk dC(Bz) Controlled Pore Glass (CPG) for users to pack their own columns for synthesis of oligonucleotides <50 bases in length.

• Proprietary design and manufacturing methods ensure consistency and high quality
• The 500 angstrom pore size of the support allows synthesis of oligonucleotides of up to 50 bases
• Save time and money

For Research Use Only. Not for use in diagnostics procedures.

GeneArt™ High-Order Genetic Assembly System Invitrogen™

The GeneArt® High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and recombine DNA fragments with high efficiency. This greatly reduces the in vitro handling of DNA and eliminates the need for enzymatic treatments, such as restriction and ligation, while allowing for precise fusions of DNA sequences. The kit contains materials for the transformation and purification from yeast (no growth media), and competent E. coli for plasmid amplification of correct clones.

Easy and Powerful — Clone up to 10 DNA fragments, with the sequence of your choice, simultaneously in a single vector (up to 110 Kbp); no restriction digestion, ligation or recombination sites required
Precision and Efficiency —Designed to let you clone what you want, where you want, in the orientation you want, and achieve up to 90% correct clones with no extra sequences left behind
Flexibility — Use our linear vector, a vector of your choice, or clone pre-existing DNA fragments that have no end-homology without further modifications
Free Tools — Design DNA oligos and more with our free web-based interface that walks you through your project step-by-step
Diverse Applications — Streamline many synthetic biology and molecular biology techniques through the rapid combination, addition, deletion, or exchange of DNA segments

For the cloning of 1 to 4 DNA fragments of limited size, and if you prefer an in vitro approach, consider using the GeneArt® Seamless Cloning and Assembly Kit (cat # A13288).

Easily Create New Specific Constructs from Diverse DNA Fragments
The GeneArt® High-Order Genetic Assembly System takes advantage of transformation-associated recombination (TAR) in the yeast Saccharomyces cerevisae to join pre-existing DNA fragments, or chemically synthesized oligonucleotides, into a single recombinant molecule. DNA fragments and linearized vector are joined based on shared end-terminal homology. If no such end homology exists between pieces, they can be “stitched" together with recombination linkers, synthetic DNA oligonucleotides that provide end-terminal homology between two unrelated DNA fragments. The process is very efficient and seamless, leaving no extra sequences after the assembly. Even though it has been shown to work for up to 0.5 Mb and 50 DNA fragments, this product has been optimized for up to 110 Kb and 10 DNA fragments in a vector.

Simple Clone Verification
In order to minimize the work that is done in yeast, recombinant yeast clones are subjected to a simplified 10-minute DNA extraction protocol. The extraction step yields assembled molecule in enough quantity to do colony PCR verification of the junctions, as well as direct transformation of E.coli cells for downstream analysis. The proprietary lysis buffer and glass beads needed for the extraction are included in the kit. For selection of recombinant yeast clones we offer a selective yeast media kit, CSM Media for Mav203 Yeast Cells (cat# A13292).

Considerations for Choosing a Cloning Vector
The GeneArt® High-Order Genetic Assembly System requires shuttle vectors that have high capacity and are compatible with yeast and E.coli (i.e. BAC-YAC shuttle vectors). There is no cloning vector included in this product, but we offer a ready-to-use linear cloning vector separately called the GeneArt® pYES1L Vector with Sapphire Technology™ (cat# A13287). You can also use your own vector, but it must be compatible with the High-Order Genetic Assembly System. This compatibility can be easily accomplished with our GeneArt® High-Order Vector Conversion Cassette (cat#A13291).

Cloning Efficiency
In GeneArt® High-Order Assembly the main factors affecting cloning efficiency are the size of the DNA elements, the number of those without end-homology, the total size of the final molecule, and the quality and specificity of the fragment. The terminal end of the PCR fragments (A-overhangs or blunt), does not affect the cloning efficiency.

Typical cloning efficiencies for different numbers of fragments with end-homology assembled into the GeneArt® pYES1L Vector with Sapphire Technology™ are the following:
• >90% for 5 DNA fragments of 10 Kb each
• >90% for 10 DNA fragments of 5 Kb each
• >50% for 10 DNA fragments of 10 Kb each

Common cloning efficiencies for pre-existing fragments, without end-homology, assembled into the GeneArt® pYES1L Vector with Sapphire Technology™ using 'stitching’ DNA oligonucleotides are:
• >90% for 1 fragment of 10 Kb
• >75% for 2 DNA fragments of 10 Kb each

Design Your Cloning in silico
A key step in GeneArt® High-Order Genetic Assembly is the correct design of fragments and oligos with the appropriate homology and spacing to ensure successful assembly of your clone. To simplify and speed the design process we provide a free online design tool to help you design your experiment in silico. The tool checks for compatibility of the experimental design with the product specifications, designs DNA oligos for either PCR amplification or for stitching of the different elements to clone, and presents the user with a graphical representation of the vector as well as a downloadable annotated sequence in GenBank format that is compatible with Vector NTI® software.

Applications
The GeneArt® High-Order Genetic Assembly System is designed to empower cloning and DNA assembly experiments in a wide range of molecular biology and synthetic biology applications, among others. The product allows for the creation of modular expression vectors, with interchangeable parts, and can be used to perform a variety of tasks that would otherwise involve multiple steps. Use the kit to simply: construct fusion proteins, delete, replace, or add DNA elements such as restriction sites, clone large pre-existing DNA fragments without end-homology, and many other techniques that require manipulation of genetic sequences.

dG(dmf) FastPhoramidite™ Reagent Applied Biosystems™

The dG(dmf) FastPhoramidite® is used for nucleic acid synthesis on the Expedite™ 8905, 8909, and 8909 with MOSS upgrade DNA synthesizers. Our β-cyanoethyl phosphoramidites guarantee you high-quality nucleic acid synthesis.

• Proprietary manufacturing processes guarantee high purity products.
• Expedite™ system fast deprotecting amidites offer fast deprotection for primer-length oligonucleotides, shortening overall production time and costs.
• Fast deprotecting amidites and standard deprotecting amidites are use-tested and optimized for use on the Expedite™ System for high-quality nucleic acid synthesis.
• Phosphoramidite® kits simplify ordering, saving you time and money.

High purity
Our phosphoramidites benefit from proprietary manufacturing processes that yield products of high purity. In synthesis they perform at correspondingly high levels, producing pure, high quality DNA at all scales. We guarantee that 5'-dimethoxytrityl nucleoside β-cyanoethyl phosphoramidites, monomer intermediates for licensed β-cyanoethyl phosphoramidite chemistry, will produce DNA that is at least 99.8% chemically authentic when used with recommended synthesis cycles.

For Research Use Only. Not for use in diagnostics procedures.
Results per page
    spinner