Nucleic Acid Introduction Reagents

Nucleic acid introduction reagents are products and kits used to introduce nucleic acids into cells, yeast, bacteria, and other microorganisms via transfection and chemical transformations. These products include reagents and kits for transfection, chemical transformation, and electroporation transformation.
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FreeStyle™ MAX Reagent Gibco™

FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins.

BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control Invitrogen™

The BLOCK-iT™ Alexa Fluor™ Red Fluorescent Control is an ideal indicator of lipid-mediated transfection efficiency for RNAi experiments, particularly those using the Lipofectamine™ RNAiMAX Transfection Reagent.

Lipofectin™ Transfection Reagent Invitrogen™

Lipofectin® Transfection Reagent is the reagent of choice for transfection of endothelial cells. Lipofectin® Transfection Reagent is also suitable for transfecting DNA, RNA, and oligonucleotides into mammalian cells, and DNA into plant protoplasts.

BLOCK-iT™ Fluorescent Oligo, for electroporation Invitrogen™

BLOCK-iT™ Fluorescent Oligo provides: • A strong, easily detectable FITC signal indicating transfection efficiency • Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA • Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA...

Lipofectamine™ RNAiMAX Transfection Reagent Invitrogen™

Lipofectamine™ RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine™ RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of...

BLOCK-iT™ Transfection Optimization Kit Invitrogen™

The BLOCK-iT™ Transfection Optimization Kit is designed to optimize RNAi transfection using controls for transfection and viability. Each kit contains a BLOCK-iT™ Fluorescent Oligo, which provides: • A strong, easily detectable FITC signal indicating transfection efficiency • Chemical modifications...

Jump-In™ GripTite™ HEK 293 Kit Thermo Scientific™

The Jump-In™ GripTite™ HEK293 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ GripTite™ HEK293 cell line, to create an isogenic stable cell line with less effort and in less time than traditional cell engineering methods.

ExpiFectamine™ 293 Transfection Kit Gibco™

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells.

Jump In™ T-REx™ CHO-K1 Kit Thermo Scientific™

The Jump-In™ T-REx™ CHO-K1 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ CHO-K1 cell line, to create an inducible isogenic stable cell line with less effort and in less time than traditional cell engineering methods.

Lipofectamine™ 3000 Transfection Reagent Invitrogen™

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Lipofectamine™ MessengerMAX™ Transfection Reagent Invitrogen™

Lipofectamine™ MessengerMAX mRNA Transfection Reagent delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved application outcomes and more biologically relevant research.

LentiArray™ CRISPR Negative Control Lentivirus, human, non-targeting Invitrogen™

Negative control gRNAs are essential in screening applications for setting thresholds for hit determination. The Invitrogen™ LentiArray™ CRISPR Negative Control Lentivirus, Human Scrambled, contains a gRNA sequence with no sequence homology to any region of the human genome.

LentiArray™ CRISPR Positive Control Lentivirus, human HPRT Invitrogen™

Optimizing delivery conditions is a critical step in any experiment utilizing the CRISPR/Cas9 system. The Invitrogen™ LentiArray™ CRISPR Positive Control Lentivirus gRNA against HPRT is a highly efficient gRNA validated in multiple human cell lines that has achieved editing efficiencies of greater...

LentiArray™ Cas9 Lentivirus Invitrogen™

LentiArray Cas9 Lentivirus provides an efficient method to drive high level Cas9 nuclease expression in a wide variety of cell types. The LentiArray Cas9 Lentivirus construct includes a human codon-optimized version of Cas9 with two nuclear localization signals to facilitate efficient delivery into...

LentiArray™ Cas9 Lentivirus Invitrogen™

LentiArray Cas9 Lentivirus provides an efficient method to drive high level Cas9 nuclease expression in a wide variety of cell types. The LentiArray Cas9 Lentivirus construct includes a human codon-optimized version of Cas9 with two nuclear localization signals to facilitate efficient delivery into...

ExpiFectamine™ CHO Transfection Kit Gibco™

The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells.

Neon™ Transfection System Invitrogen™

The Neon™ Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in...

Neon™ Transfection Tubes Invitrogen™

The Neon™ Transfection Tubes are designed specifically for use with the Neon™ Transfection System device (MPK5000). Each Neon™ Transfection System Kit (MPK1096 and MPK10096) already comes with 20 electroporation tubes.

PichiaPink™ Secretion Signal Set Invitrogen™

The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors.

Competent Cell Sampler Invitrogen™

The Competent Cell Sampler kit contains a pair of four different strains of chemically competent E. coli cells in convenient One Shot formats. Each single-use vial of cells is ready to use, with no extra pipetting steps or freeze-thaw cycles which reduce transformation efficiency.

PichiaPink™ Secretion Optimization Kit Invitrogen™

The PichiaPink™ Secretion Optimization Kit is a new recombinant protein expression system based on the yeast Pichia pastoris. The PichiaPink™ Secretion Optimization Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein.

Jump-In™ Fast Gateway™ Core Kit Invitrogen™

The Jump-In™ Fast Gateway™ Core Kit is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites).

Jump-In™TI™ Platform Kit Invitrogen™

The Jump-In™TI™ Platform Kit enables generation of a Jump-In™TI™ cell line. A Jump-In™TI™ cell line is created by co-transfecting one of three vectors containing the R4 attP target site with the transiently expressed vector for the PhiC31 integrase into your mammalian cell line.

ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System Invitrogen™

The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway™ technologies to facilitate easy...

Pichia Expression Kit, original kit Invitrogen™

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium.

Neon™ Transfection System Starter Pack Invitrogen™

The Neon™ Transfection System Starter Pack contains a Neon™ Transfection device and two 192 reaction kits (10 µL and 100 µL sample sizes) to get you started in a fast, convenient, and cost effective manner.

Neon™ Transfection System 100 µL Kit Invitrogen™

The Neon™ Transfection System 100 µL Kit is designed specifically for use with the Neon™ Transfection System. Use this kit for transfection volumes of 100 µL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells.

Multi-Copy Pichia Expression Kit Invitrogen™

The Multi-Copy Pichia Expression Kit contains the pPIC3.5K, pPIC9K, and pAO815 vectors for production and selection of Pichia strains that contain more than one copy of the gene of interest. In many cases, increased copy number has led to increased expression levels. pPIC9K and pPIC3.

FreeStyle™ 293 Expression System Invitrogen™

The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1).

MAX Efficiency™ Transformation Reagent for Algae Invitrogen™

MAX Efficiency™ Transformation Reagent for Algae is a buffer that enhances transformation efficiency for multiple strains of Chlamydomonas species. One of the biggest hurdles in research and development with Chlamydomonas has been the introduction of exogenous DNA into Chlamydomonas strains due to...

siPORT™ Amine Transfection Agent with manual Invitrogen™

A polyamine-based Ambion™ siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 1 mL.

LV-MAX™ Transfection Kit Gibco™

The Gibco LV- MAX Transfection Kit is an essential component of the LV-MAX Lentiviral Production System. It is formulated for the transient transfection of Gibco Viral Production Cells (high-density suspension-adapted human embryonic kidney (HEK) 293-derived cells).

CTS™ LV-MAX™ Transfection Kit Gibco™

The Gibco CTS LV-MAX Transfection Kit is an essential component of the CTS LV-MAX lentiviral production system. Based on extensive Design of Experiment (DOE) methodologies, the cationic lipid-based transfection reagent, lentiviral production supplement, and LV enhancer are specifically screened for...

Silencer™ siRNA Starter Kit Invitrogen™

Perfect for researchers new to RNAi, this Ambion™ kit contains all of the reagents, protocols, and instructions necessary to get underway with siRNA transfection and ensure successful gene knockdown experiments.

Cellfectin™ II Reagent Gibco™

Cellfectin® II Reagent is a cationic-lipid formulation designed for optimal transfection of insect cells. Cellfectin® II is an improved version of the original Cellfectin® Reagent. In addition to providing the same great performance as Cellfectin®, Cellfectin® II Reagent is manufactured to ensure...

DMRIE-C Transfection Reagent Invitrogen™

DMRIE-C Transfection Reagent is suitable for transfecting DNA and RNA into eukaryotic cells, and is particularly effective for transfecting suspension cells (e.g., Jurkat) and other lymphoid-derived cell lines. Refer to the Cell Lines database at www.invitrogen.

Optifect™ Transfection Reagent Invitrogen™

Optifect™ Transfection Reagent is a pre-optimized formulation designed to ensure highly efficient gene delivery to a wide variety of cell lines transfected at low confluence or density (10%-70%) (Figure 1).

Lipofectamine™ 2000 CD Transfection Reagent Invitrogen™

Lipofectamine™ 2000 CD (Chemically Defined) Transfection Reagent is a proprietary animal origin-free formulation for transfecting nucleic acids into eukaryotic cells. Using Lipofectamine™ 2000 CD reagent for transfection provides the following advantages: • Highest transfection efficiency in many...

Oligofectamine™ Transfection Reagent Invitrogen™

Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells. Oligofectamine™ Transfection Reagent forms stable complexes with oligos, permitting efficient transfection into eukaryotic cells in a highly...

Invivofectamine™ 3.0 Reagent Invitrogen™

Invivofectamine™ 3.0 Reagent is an animal origin-free lipid nanoparticle designed for high efficiency in vivo delivery of siRNA and miRNA to mouse liver cells following tail vein injection. Using Invivofectamine™ 3.0 Reagent Invivofectamine™ 3.

Calcium Phosphate Transfection Kit Invitrogen™

The Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation. How it works The calcium phosphate transfection method for introducing DNA into mammalian cells is based on forming a calcium...

PEI-Transferrinfection Kit Invitrogen™

Transfection of DNA into eukaryotic cells is a common method to study biological mechanisms. A major goal is the efficient and specific delivery of genes into the desired target cells. Although a wide panel of techniques and vectors (viral and nonviral) have been developed that work with variable...

Opti-Plex™ Complexation Buffer Gibco™

Opti-Plex Complexation Buffer is a cGMP-manufactured, chemically defined, animal origin-free (AOF), methionine-free, biotin-free, low-protein transfection complexation buffer based on Opti-MEM I Reduced Serum Medium.

ExpiFectamine™ 293 Met (–) Transfection Kit Gibco™

The ExpiFectamine 293 Met (–) Transfection Kit is a core component of the Expi293 Protein Labeling Kit for rapid, high-yield transient protein expression from Expi293F cells for applications where labelling of methionine residues in proteins is desired.

Viral-Plex™ Complexation Buffer Gibco™

Gibco Viral-Plex Complexation Buffer is a chemically defined, protein-free, phenol red-free buffer for complexing plasmid DNA with transfection reagent for standard transfection applications.

CTS™ Xenon™ Electroporation Buffer, bag Gibco™

Gibco CTS Xenon Electroporation Buffer is designed specifically for use with the Xenon Electroporation System. It is a universal buffer that supports the transfection of a variety human primary cells and payloads (e.g.

CTS™ Xenon™ Electroporation Buffer, bottle Gibco™

Gibco CTS Xenon Electroporation Buffer is designed specifically for use with the Xenon Electroporation System. It is a universal buffer that supports the transfection of a variety human primary cells and payloads (e.g.

CTS™ Xenon™ Genome Editing Buffer, bag Gibco™

Gibco CTS Xenon Genome Editing Buffer is designed specifically for use with the Xenon Electroporation System. It has been designed to improve performance with gene editing -pecific payloads (e.g., CRISPR Cas9) for knock-out- or knock-in-based applications in a variety human primary cells.

AAV-MAX Transfection Kit Gibco™

The Gibco AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0. The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production...

Lipofectamine™ Stem Transfection Reagent Invitrogen™

Lipofectamine Stem Transfection Reagent is optimized to achieve maximum efficiency with minimal early differentiation in a wide range of stem cells. It can co-deliver DNA, RNA, and Cas9 ribonucleoprotein (RNP) complexes.

EasySelect™ Pichia Expression Kit Invitrogen™

The EasySelect™ Pichia Expression Kit provides all needed components for protein production in the yeast Pichia pastoris. The EasySelect™ Kit contains Pichia yeast strains, expression vectors, and reagents that enable transformation of Pichia cells.

FreeStyle™ CHO-S Cells Gibco™

FreeStyle™ CHO-S Cells are part of the FreeStyle™ MAX Expression System, which is a breakthrough technology for rapid and high-yield mammalian protein production. This cell line is adapted to high density, serum-freesuspension culture in FreeStyle™ CHO Expression Medium and iscapable of producing...

FreeStyle™ 293-F Cells Gibco™

The FreeStyle™ 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle™ MAX 293 Expression System or FreeStyle™ 293 Expression System. FreeStyle™ 293-F cells are adapted to suspension culture in FreeStyle™ 293 Expression Medium.

BLOCK-iT™ Transfection Kit Invitrogen™

The BLOCK-iT™ Transfection Kit contains everything you need for successful high-efficiency transfection of siRNA into cells. Each kit contains Lipofectamine™ 2000 Transfection Reagent for efficient delivery in a broad range of cell lines and the BLOCK-iT™ Fluorescent Oligo for use as an indicator of...

Lipofectamine™ LTX Reagent Invitrogen™

Lipofectamine™ LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells, resulting in the highest possible transfection efficiencies and viabilities.

293fectin™ Transfection Reagent Gibco™

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No.

PLUS™ Reagent Invitrogen™

PLUS™ Reagent is used in conjunction with various transfection reagents, such as Lipofectamine™ Reagent, to improve transfection efficiency in adherent cell lines. Using PLUS™ Reagent Some customers find that when using PLUS™ Reagent, less optimization of DNA concentration is needed to achieve high...

Lipofectamine™ 2000 Transfection Reagent Invitrogen™

Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine™ 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene...

Lipofectamine™ Transfection Reagent Invitrogen™

Lipofectamine™ Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine™ Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with...

Neon™ Transfection System 10 µL Kit Invitrogen™

The Neon™ Transfection System 10 µL Kit is designed specifically for use with the Neon™ Transfection System. Use this kit for transfection volumes of 10 µL, containing 5 × 104–2 × 105 adherent cells or 1 × 105–5 × 105 suspension cells.
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