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Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent Invitrogen™

Lipofectamine CRISPRMAX Cas9 Transfection Reagent is the first optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery. It is a high-throughput-friendly, cost-effective alternative to electroporation.

Working together with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA, it provides:
• Demonstrated cleavage efficiency—tested in over 20 cell types including iPSC, mESC, N2A, CHO, A549, HCT116, HeLa, HEK293, and several others
• Low cell toxicity—less cells needed to initiate your experiment
• High throughput friendly—an ideal delivery solution for 96-well format
• Cost savings—whether cost-per-reaction or initial investment

Achieve high transfection efficiency in novel genome editing applications
Lipofectamine CRISPRMAX transfection reagent increases the likelihood of successful cleavage and recombination, especially when combined with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA. This ultimately maximizes the efficiency of performing genetic modifications.

Improve experimental outcomes using TrueCut Cas9 Protein v2
Unlike CRISPR plasmid or Cas9 mRNA, using Cas9 protein provides superior cleavage efficiency in primary cells and stem cells. It eliminates the need for transcription or translation of the payload, removes the risk of genomic integration, and is cell cycle independent.

Lipofectamine™ Transfection Reagent Invitrogen™

Lipofectamine® Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine® Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with many cell lines.

With Lipofectamine® Transfection Reagent, you'll get:

• A reagent proven to work in high-throughput applications
• A reliable reagent for establishing stable cell lines
• A reagent that works well with PLUS™ Reagent for higher protein expression

A proven technology
Our Lipofectamine®-brand reagents have been recognized as the most-cited transfection reagent family, with tens of thousands of citations since launch in 1993. Lipofectamine® Transfection Reagent is no exception as a trusted reagent for a wide range of cell lines. When used in combination with PLUS™ Reagent, Lipofectamine® Transfection Reagent has been shown to work well with cells such as BHK-21, NIH 3T3, COS-1, fibroblasts, keratinocytes, HT-29, MRC-5, and SK-BR3.

A 'value' alternative for plasmid DNA
With such a legacy comes a commitment to providing the best reagents for all scientists and labs, regardless of their funding capabilities. Lipofectamine® Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol. For optimal results in a wider range of plasmid DNA transfection conditions, we recommend Lipofectamine® LTX Reagent, due to its optimal balance of potency & low-cytotoxicity. Lipofectamine® 2000 Transfection Reagent is recommended if you are transfecting a broad range of cell lines or want to transfect RNA.

ExpiFectamine™ 293 Transfection Kit Gibco™

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium

Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
Provides robust and reproducible transfection results, giving users greater confidence in their results
Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields

The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 16 to 18 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.

Lipofectamine™ 2000 Transfection Reagent Invitrogen™

Lipofectamine® 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine® 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.

With Lipofectamine® 2000 Transfection Reagent, you'll get:

• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines

A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine® 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine® 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine® 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine® 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.

Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine® 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.

PichiaPink™ Secretion Optimization Kit Invitrogen™

The PichiaPink™ Secretion Optimization Kit is a new recombinant protein expression system based on the yeast Pichia pastoris. The PichiaPink™ Secretion Optimization Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secretion Optimization Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein.

•   Get a complete kit to get started with protein production in Pichia pastoris.

•   Screen your Pichia transformants rapidly by auxotrophy and color selection.
•   Reduce protein degradation with protease-deficient Pichia pastoris strains.
•   Optimize protein secretion with one of 8 secretion signal sequences.

The PichiaPink™ Secretion Optimization Kit comes with:
•   PichiaPink™ Vector Kit (Cat. No. A11152)
•   PichiaPink™ Secretion Signal Set (Cat. No. A11155)
•   PichiaPink™ Expression Strain Set (Cat. No. A11154)
•   PichiaPink™ Media Kit (Cat. No. A11156)

A New Way to Screen Pichia Transformants
The PichiaPink™ Secretion Optimization Kit uses adenine auxotrophy to select for Pichia transformants. The PichiaPink™ strains have the ade2 genotype and require complementation with the ADE2 gene to grow without adenine. Use of an auxotrophic marker also makes it easier to maintain transformants. No antibiotics are required to maintain your transformants.

In addition, you can screen for Pichia transformants by color. The untransformed PichiaPink™ strains appear as pink colonies while transformed PichiaPink™ strains appear as white colonies (Fig 1).

New Expression Vectors for Pichia
The PichiaPink™ Secretion Optimization Kit comes with the new PichiaPink™ Vector Kit. The kit includes two vectors, pPINK-LC and pPINK-HC. The pPINK vectors are built around the ADE2 gene for complementing the ade2-deficient Pichia strains. Both vectors use the methanol-induced AOX1 promoter to express your protein. The pPINK-LC integrates at low-copy number into the Pichia genome while the pPINK-HC vector integrates with high-copy number.

Both pPINK-LC and pPINK-HC vectors are compatible with the PichiaPink™ Secretion Signal Set. Using a 3-way ligation, you can add one of 8 secretion sequences to your gene. Note: the pPINK vectors express proteins intracellularly by default.

Both the PichiaPink™ Vector Kit (Cat. No. A11152) and PichiaPink™ Secretion Signal Set (Cat. No. A11155) can be ordered separately to refill the PichiaPink™ Secretion Optimization Kit.

Keep Your Proteins Intact
The PichiaPink™ Secretion Optimization Kit comes with the PichiaPink™ Expression Strain Set. This set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Expression System. All 4 strains have the ade2 genotype. In addition, 3 strains include knockouts in 2 protease genes. Protease-deficient strains reduce the need for protease inhibitors when growing your cells and improve the yield of your protein. Test a single protease knockout or the double protease knockout to find the one that works best for you.

You can order the PichiaPink™ Expression Strain Set (Cat. No. A11154) separately to refill the PichiaPink™ Secretion Optimization Kit.

Media Pouches to Get You Started

The PichiaPink™ Media Kit is a set of convenient prepackaged media pouches for use with the PichiaPink™ Secretion Optimization Kit. The PichiaPink™ Media Kit contains media needed to grow PichiaPink™ strains from frozen stocks to cultures ready for transformation and selection. You can order the PichiaPink™ Media Kit (Cat. No. A11156) separately to refill the PichiaPink™ Secretion Optimization Kit.

Why Choose the PichiaPink™ Yeast Expression System?
The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.

For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links
Learn more about the PichiaPink™ Yeast Expression System.
Learn more about our other protein expression systems.

References

1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]
2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]

siPORT™ Amine Transfection Agent Invitrogen™

A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 1 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used.

Accessory Products:
siPORT™ NeoFX™ Transfection Agent (SKU#s AM4511 and AM4510), a cationic and neutral lipid mixture, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.

293fectin™ Transfection Reagent Gibco™

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018), and is intended for use with the FreeStyle™ 293 Expression System (Cat. No. K9000-01). 293fectin™ Transfection Reagent provides the following advantages:

• Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
• Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
• Add 293fectin™ reagent complexes directly to cells in culture medium
• No need to remove complexes or change or add medium following transfection

ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System Invitrogen™

The ViraPower™ HiPerform™ T-REx™ Gateway® Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway® technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features
• WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, increases transgene expression and cPPT (central Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
• Blasticidin selection marker for stable selection under control of SV40 promoter

Kit Includes
• ViraPower™ HiPerform™ T-Rex™ Gateway® Vector Kit (Cat # A11144)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
• Gateway® LR Clonase® II Plus Enzyme Mix (Cat # 12538120)
• One Shot® Stbl3™ Chemically Competent E. coli (Cat # C737303)
• Geneticin® (Cat # 10131035)
• pENTR™ Gus positive control plasmid

For research use only. Not intended for any therapeutic or diagnostic use.

Neon™ Transfection System 100 µL Kit Invitrogen™

The Neon™ Transfection System 100 µL Kit is designed specifically for use with the Neon™ Transfection System. Use this kit for transfection volumes of 100 µL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100 µL Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.

PichiaPink™ Secretion Signal Set Invitrogen™

The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors.

Optimize Secretion of Your Expressed Protein
By adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastoris secretes few native proteins, so the bulk of the protein in the media will be yours.

Each DNA sequence encodes a different secretion signal. You can test each secretion signal sequence to find the one that works best for your protein.

The PichiaPink™ Secretion Signal Set comes with 8 different secretion signals including:
•     α-mating factor pre-sequence from Saccharomyces cerevisiae
•     α-amylase signal sequence from Aspergillus niger
•     Glucoamylase signal sequence from Aspergillus awamori
•     Serum albumin signal sequence from Homo sapiens
•     Inulinase signal sequence from Kluyveromcyes maxianus
•     Invertase signal sequence from Saccharomyces cerevisiae
•     Killer protein signal sequence from Saccharomyces cerevisiae
•     Lysozyme signal sequence from Gallus gallus

Easily Add a Secretion Signal to Your Protein
The DNA fragments in the PichiaPink™ Secretion Signal Set are designed to be easily inserted in front of your gene when using the pPINK-HC or pPINK-LC vectors. You can try different secretion signal sequences to see which one gives optimal secretion of your protein.

Each signal sequence includes the Kozak sequence for the AOX1 gene from Pichia pastoris to ensure efficient translation of your protein.

The secretion signal sequences come as phosphorylated duplex oligomers in 40 pmol aliquots. Resuspend oligomers in 40 µl TE buffer before use.

Why Choose the PichiaPink™ Yeast Expression System?
The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.

For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links
Learn more about the PichiaPink™ Yeast Expression System.
Learn more about our other protein expression systems.

References

1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]
2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]

Lipofectamine™ RNAiMAX Transfection Reagent Invitrogen™

Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types.

With Lipofectamine® RNAiMAX Transfection Reagent you will get:

• Superior transfection efficiency requiring lower RNAi concentrations, leading to more effective gene knockdown with minimal nonspecific effects
• Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
• Superior transfection efficiencies for miRNA antagonists and mimics
• Compatibility with a broad range of cell types, providing the most versatile approach to all of your gene silencing experiments
• A simple and rapid protocol for consistent and reproducible results

High knockdown in a wide range of cells
Lipofectamine® RNAiMAX Transfection Reagent transfects a wide range of cell types (see figure). For gene silencing, Lipofectamine® RNAiMAX Transfection Reagent's high-efficiency transfections lead to the high levels of gene knockdown needed to achieve convincing results.

Simple, high-throughput–ready transfections
Simply mix Lipofectamine® RNAiMAX Transfection Reagent with siRNA, add to your cells, incubate, and measure gene knockdown. The simplicity and speed combined with high transfection efficiency make Lipofectamine® RNAiMAX Transfection Reagent ideal for high-throughput siRNA transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.

Find an optimized Lipofectamine® RNAiMAX transfection protocol for your cell line.

siPORT™ NeoFX™ Transfection Agent with manual Invitrogen™

This Ambion® lipid-based formulation can be used to efficiently transfect adherent cells with siRNA as they are subcultured—without increased cytotoxicity. It is provided in one tube containing 1 mL.

• Versatile—works with a broad range of cell lines
• Reproducible—produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
• Efficient—performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects
• Fast—reverse-transfects cells as they are plated, saving a day

Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT™ NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. From start to finish, successful gene silencing experiments can be completed in as early as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.

Reverse-Transfect Cells as They are Plated, Saving a Day
Just add siPORT™ NeoFX to your diluted siRNA, incubate to form transfection complexes, add the complexes to the culture wells, and overlay with cells. This new streamlined protocol, named "neofection", will save researchers a full day of valuable time when compared to traditional plated transfection procedures. The transfection complexes are active and stable even in the presence of serum, so there is no need to remove or replace media following transfection.

Optimized for Lower siRNA Concentrations and High Reproducibility
Recent reports indicate that siRNA concentrations of 100 nM or higher can lead to nonspecific changes in gene expression (off-target effects) in mammalian cultured cells. Reducing the amount of siRNA used for transfections to 1–20 nM minimizes these nonspecific effects, while still providing extensive silencing of the target gene. siPORT™ NeoFX Transfection Agent efficiently transfects low concentrations of siRNA. Furthermore, this agent delivers high performance among multiple wells for replicate samples and between different lots of the transfection agent.

Compatible with a Broad Range of Cell Lines
The agent has been used successfully to deliver siRNA into many cell types, including MCF-7 (human breast adenocarcinoma), HT-29 (human colorectal adenocarcinoma), HeLa-S3 (human cervix adenocarcinoma), HeLa (human cervix adenocarcinoma), HepG2 (human hepatocellular carcinoma), BJ (human foreskin fibroblast), A549 (human lung carcinoma), UMR106 (rat osteosarcoma), SKOv3 (human ovarian carcinoma), and SKNAS (human bone marrow neuroblastoma).

Accessory Products:
siPORT™ Amine Transfection Agent (SKU#s AM4502 and AM4503), a polyamine agent, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.

Neon™ Transfection System Starter Pack Invitrogen™

The Neon® Transfection System Starter Pack contains a Neon® Transfection device and two 192 reaction kits (10 µL and 100 µL sample sizes) to get you started in a fast, convenient, and cost effective manner. The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:

Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
Simple—easy to use, with a single reagent kit for all cell types
Versatile—open system allows electroporation parameters to be optimized freely

One-of-a-kind electroporation
Unlike standard cuvette-based electroporation chambers, the Neon® system uses a patented, biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows better maintenance of physiological conditions, resulting in very high cell survival compared to conventional electroporation (see figure).

Plug-n-play out of the box
The transfection process couldn’t be simpler. Draw up the cells and plasmid mix into the Neon® pipette tip, plug into the pipette holder, and press start. Then just pipette the transfected cells into your culture vessel. No more filling and pulling sample from the cuvette and capping and de-capping. The Neon® system uses a simple 3-step transfection procedure. The transfection occurs in the Neon® pipette tip.

Simplified transfection kit for all cell types
Avoid the hassle of determining which proprietary buffer kit will work with your favorite cell type. We have simplified your work with just one transfection kit that is compatible with all cell types. Start your transfection with our optimized protocols for popular cell types, or follow our standard simple optimization procedure for all new cell types.

Perform more transfections with limited or precious cells
Your cells may be limited when you are required to perform neuronal preps or when enriching for homogeneous cell populations via FACS or Dynabeads® enrichment. In these instances, reducing the number of cells required per transfection can allow you to perform more complex experiments with your precious cells.

Silencer™ siRNA Transfection II Kit Invitrogen™

This Ambion® kit is for the optimization of transfection protocols for specific cell types. It includes 0.4 mL of each of two transfection agents; siPORT™ Amine Transfection Agent and siPORT™ NeoFX™ Transfection Agent along with control siRNAs.
• Broad cell line compatibility-kit includes siPORT™ Amine and siPORT™ NeoFX™ transfection agents
• Faster protocol optimization-kit includes ready-to-transfect GAPDH and negative siRNA controls The choice of transfection agent is critical for gene silencing experiments. Every transfection agent has a different delivery efficiency when used with different cell types, and the overall transfection efficiency and degree of gene silencing depend on the nature of the transfection agent/siRNA complex. Whether you are new to RNAi research or you want to expand your experiments to include different cell lines, the Silencer® siRNA Transfection II Kit enables you to optimize your transfection protocols for specific cell types. The Silencer® siRNA Transfection II Kit includes siPORT™ Amine Transfection Agent (a polyamine mixture) and the new siPORT™ NeoFX™ Transfection Agent (a cationic and neutral lipid mixture). These reagents support efficient, reproducible siRNA transfections in different cell types. Furthermore, both can be used to transfect cells during subculturing-saving a full day of valuable time-without increased cytotoxicity. In addition to containing a choice of transfection agents, this kit includes a well-characterized GAPDH synthetic siRNA that can be used to optimize transfection conditions. Results can also be verified at the protein level with GAPDH-specific antibodies. A scrambled, negative control siRNA is also provided to confirm the specificity of the siRNA of interest and to demonstrate the absence of nonspecific, toxic effects.

Lipofectamine™ MessengerMAX™ Transfection Reagent Invitrogen™

Lipofectamine® MessengerMAX mRNA Transfection Reagent delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved application outcomes and more biologically relevant research. That's because our novel lipid nanoparticle technology is optimized to deliver the highest amount of mRNA possible without the nuclear entry step that is required with DNA.

Successfully transfect more predictive cell models with a reagent that provides:
• Amazing transfection efficiency in neurons and primary cell types
• Faster protein expression with no risk of genomic integration
• Up to 10x higher cleavage using mRNA CRISPRs

Enable high transfection efficiency in novel genome editing applications
Lipofectamine® MessengerMAX™ reagent helps increase the likelihood of successful cleavage and recombination with GeneArt® CRISPR Nuclease mRNA through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Efficiently transfect difficult-to-transfect cells
Lipofectamine® MessengerMAX™ reagent is designed to transfect a higher amount of mRNA into neurons and a broad spectrum of difficult-to-transfect primary cells. This eliminates the need for electroporation or viruses, and provides a > 2-fold improvement in transfection efficiency compared to other lipid based reagents.

Leverage advantages of mRNA transfection
Transfection of mRNA does not require nuclear uptake, which results in faster protein expression than DNA transfection since translation of mRNA occurs in the cytoplasm. Also, mRNA has no risk of genomic integration and transfection efficiency is cell cycle independent.
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