Shop All Transfection Reagents

For Use With (Equipment)


RNAi Type


Transformation Efficiency


Expi293™ Inducible Expression System Kit Gibco™

The Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The Expi293F Inducible cell line was established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. This cell line is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins.

Expi293 Inducible Expression System Kit features include:
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

The components included in the Expi293 Inducible Expression System Kit are: one vial of frozen Expi293F Inducible cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one pcDNA5/TO Mammalian Expression Vector kit, 500 mg tetracycline hydrochloride, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible Expression System are also available for individual purchase.

Lipofectamine™ 2000 Transfection Reagent Invitrogen™

Lipofectamine® 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine® 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.

With Lipofectamine® 2000 Transfection Reagent, you'll get:

• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines

A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine® 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine® 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine® 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine® 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.

Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine® 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.

Jump-In™TI™ Platform Kit Invitrogen™

The Jump-In™TI™ Platform Kit enables generation of a Jump-In™TI™ cell line. A Jump-In™TI™ cell line is created by co-transfecting one of three vectors containing the R4 attP target site with the transiently expressed vector for the PhiC31 integrase into your mammalian cell line.
Generating a Jump-In™TI™ cell line is the first step in creating a stable, targeted, and irreversible mammalian cell line expressing your gene of interest. For targeted and irreversible integration of your gene of interest into your Jump-In™TI™ cell line, use the Jump-In™TI™ Gateway® Vector Kit.
This kit is also a component of the Jump-In™TI™ Gateway® System.

293fectin™ Transfection Reagent Gibco™

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018), and is intended for use with the FreeStyle™ 293 Expression System (Cat. No. K9000-01). 293fectin™ Transfection Reagent provides the following advantages:

• Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
• Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
• Add 293fectin™ reagent complexes directly to cells in culture medium
• No need to remove complexes or change or add medium following transfection

Jump In™ T-REx™ CHO-K1 Kit Thermo Scientific™

The Jump-In™ T-REx™ CHO-K1 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ CHO-K1 cell line, to create an inducible isogenic stable cell line with less effort and in less time than traditional cell engineering methods. These cells stably express the tetracycline repressor protein, allowing for inducible expression of your retargeted gene of interest upon the addition of doxycycline.

The high retargeting efficiency, made possible by the R4 sites in the CHO-K1 cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest.

The Jump-In™ T-REx™ CHO-K1 Kit lets you:

• Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods
• Inducibly express your gene of interest using doxycycline
• Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs
• Generate multiple cell lines in parallel using the simplified work flow
• Easily access the technology without complicated licenses or restrictions to interpret

Save Time with Rapid and Efficient Generation of Engineered Cell Lines
With the Jump-In™ T-REx™ CHO-K1 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined workflow makes it easier to generate several cell lines at the same time. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones. In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements.

Expand Your Experimental Capabilities
The Jump-In™ T-REx™ CHO-K1 Kit allows for inducible expression of your toxic or unstable gene of interest and is the ideal solution for targets and assays where transient engineering technologies are problematic. The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway® destination vectors accept large inserts.

The kit includes:

Jump-In™ T-REx™ CHO-K1 Cells (2 vials @ 1 ml each)
pJTI™ R4 Dest CMV TO pA Vector (100 µg)--A Gateway® Destination vector that drives inducible expression of your gene of interest under control of the strong CMV promoter by inclusion of the Tet-Operon.
pJTI™ R4 Int Vector (100 µg)--Expresses the R4 integrase to facilitate the retargeting of your gene of interest into the R4 site.

Sold separately:

pJTI™ R4 CMV-TO MCS pA Vector--A multi-site cloning vector that drives inducible expression of your gene of interest under control of the strong CMV promoter by inclusion of the Tet-Operon.
pJTI™ R4 EXP CMV-TO EmGFP pA Vector--A control vector with inducible expression of GFP under control of the strong CMV promoter by inclusion of the Tet-Operon.

Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent Invitrogen™

Lipofectamine CRISPRMAX Cas9 Transfection Reagent is the first optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery. It is a high-throughput-friendly, cost-effective alternative to electroporation.

Working together with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA, it provides:
• Demonstrated cleavage efficiency—tested in over 20 cell types including iPSC, mESC, N2A, CHO, A549, HCT116, HeLa, HEK293, and several others
• Low cell toxicity—less cells needed to initiate your experiment
• High throughput friendly—an ideal delivery solution for 96-well format
• Cost savings—whether cost-per-reaction or initial investment

Achieve high transfection efficiency in novel genome editing applications
Lipofectamine CRISPRMAX transfection reagent increases the likelihood of successful cleavage and recombination, especially when combined with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA. This ultimately maximizes the efficiency of performing genetic modifications.

Improve experimental outcomes using TrueCut Cas9 Protein v2
Unlike CRISPR plasmid or Cas9 mRNA, using Cas9 protein provides superior cleavage efficiency in primary cells and stem cells. It eliminates the need for transcription or translation of the payload, removes the risk of genomic integration, and is cell cycle independent.

ExpiFectamine™ Sf Transfection Reagent Gibco™

ExpiFectamine Sf Transfection Reagent is a next-generation cationic lipid-based reagent for efficient DNA transfection of insect cells using fast, flexible protocols.

ExpiFectamine Sf Transfection Reagent:
• Delivers robust and reproducible transfection, giving you greater confidence in your results
• Enables transfection of plasmid DNA in adherent and suspension Sf9 and Sf21 cell cultures for reliable recombinant baculovirus production
• Facilitates efficient production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
• Exhibits low toxicity and does not require medium change post-transfection
• Is part of a simple, fast protocol with a short 5-minute complexation time
• Demonstrates linear scalability of transfections from culture volumes of 4 mL to 500 mL in suspension format

The ExpiFectamine Sf Transfection Reagent is a core component of the ExpiSf Expression System and has been optimized for transfection of ExpiSf9 Cells. However, the reagent also demonstrates superior performance using other Sf9 and Sf21 insect cells cultured in serum-free media such as Sf-900 II SFM or Sf-900 III SFM in both adherent and suspension culture formats.

FreeStyle™ 293-F Cells Gibco™

The FreeStyle™ 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle™ MAX 293 Expression System or FreeStyle™ 293 Expression System. FreeStyle™ 293-F cells are adapted to suspension culture in FreeStyle™ 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle™ 293 Expression Medium. The FreeStyle™ 293-F cell line is supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle™ 293 Expression Medium and 10% DMSO. Characteristics of FreeStyle™ 293-F cells:

• Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages.
• Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle™ 293 Expression Medium.
• Demonstrate high transfection efficiencies with FreeStyle™ MAX reagent.
• Suspension cultures may be transfected in FreeStyle™ 293 Expression Medium without the need to change media.
• Permits transfection of cells at large volumes.

LentiArray™ CRISPR Negative Control Lentivirus, human, non-targeting Invitrogen™

Negative control gRNAs are essential in screening applications for setting thresholds for hit determination. The Invitrogen™ LentiArray™ CRISPR Negative Control Lentivirus, Human Scrambled, contains a gRNA sequence with no sequence homology to any region of the human genome.

FreeStyle™ 293 Expression System Invitrogen™

The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or separately.

FreeStyle™ 293 Expression Medium is chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells under suspension type culture conditions (Figure 2). The medium is a complete, ready-to-use medium that has been supplemented with GlutaMAX™-I Supplement and is animal-origin free. FreeStyle™ 293 Expression Medium is able to save significant time and costs associated with adaptation of cell cultures to serum-free conditions.

Jump In™ T-REx™ HEK 293 Kit Thermo Scientific™

The Jump-In™ T-REx™ HEK293 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ HEK293 cell line, to create an inducible isogenic stable cell line with less effort and in less time than traditional cell engineering methods. These cells stably express the tetracycline repressor protein, allowing for inducible expression of your retargeted gene of interest upon addition of doxycycline.

The high retargeting efficiency, made possible by the R4 sites in the HEK293 cell line, allows for the use of isogenic pools for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest.

The Jump-In™ T-REx™ HEK293 Kit lets you:

• Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods
• Inducibly express your gene of interest using doxycycline
• Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs
• Generate multiple cell lines in parallel using the simplified work flow
• Easily access the technology without complicated licenses or restrictions to interpret

Save Time with Rapid and Efficient Generation of Engineered Cell Lines
With the Jump-In™ T-REx™ HEK293 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined protocol makes it easier to generate several cell lines at the same time. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones. In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements.

Expand Your Experimental Capabilities
The Jump-In™ T-REx™ HEK293 Kit allows for inducible expression of your toxic or unstable gene of interest and is the ideal solution for targets and assays where transient engineering technologies are problematic. The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway® destination vectors accept large inserts.

The kit includes:

Jump-In™ T-REx™ HEK293 cells (2 vials @ 1 ml each)
pJTI™ R4 Dest CMV-TO pA vector (100 µg)--A Gateway® Destination vector that drives inducible expression of your gene of interest under control of the strong CMV promoter by inclusion of the Tet-Operon.
pJTI™ R4 Int vector (100 µg)--Expresses the R4 integrase to facilitate the retargeting of your gene of interest into the R4 site.

Sold separately:

pJTI™ R4 CMV-TO MCS pA vector--A multi-site cloning vector that drives inducible expression of your gene of interest under control of the strong CMV promoter by inclusion of the Tet-Operon.
pJTI™ R4 EXP CMV-TO EmGFP pA vector--A control vector with inducible expression of GFP under control of the strong CMV promoter by inclusion of the Tet-Operon.

Lipofectamine™ 3000 Transfection Reagent Invitrogen™

Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28) 


View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Neon™ Transfection System Starter Pack Invitrogen™

The Neon® Transfection System Starter Pack contains a Neon® Transfection device and two 192 reaction kits (10 µL and 100 µL sample sizes) to get you started in a fast, convenient, and cost effective manner. The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:

Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
Simple—easy to use, with a single reagent kit for all cell types
Versatile—open system allows electroporation parameters to be optimized freely

One-of-a-kind electroporation
Unlike standard cuvette-based electroporation chambers, the Neon® system uses a patented, biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows better maintenance of physiological conditions, resulting in very high cell survival compared to conventional electroporation (see figure).

Plug-n-play out of the box
The transfection process couldn’t be simpler. Draw up the cells and plasmid mix into the Neon® pipette tip, plug into the pipette holder, and press start. Then just pipette the transfected cells into your culture vessel. No more filling and pulling sample from the cuvette and capping and de-capping. The Neon® system uses a simple 3-step transfection procedure. The transfection occurs in the Neon® pipette tip.

Simplified transfection kit for all cell types
Avoid the hassle of determining which proprietary buffer kit will work with your favorite cell type. We have simplified your work with just one transfection kit that is compatible with all cell types. Start your transfection with our optimized protocols for popular cell types, or follow our standard simple optimization procedure for all new cell types.

Perform more transfections with limited or precious cells
Your cells may be limited when you are required to perform neuronal preps or when enriching for homogeneous cell populations via FACS or Dynabeads® enrichment. In these instances, reducing the number of cells required per transfection can allow you to perform more complex experiments with your precious cells.

PEI-Transferrinfection Kit Invitrogen™

Transfection of DNA into eukaryotic cells is a common method to study biological mechanisms. A major goal is the efficient and specific delivery of genes into the desired target cells. Although a wide panel of techniques and vectors (viral and nonviral) have been developed that work with variable efficiency, most vectors lack a target cell specificity. Nonviral vectors are attractive because of their ease of manipulation, safety and high flexibility in the size of the delivered transgene. The Transferrinfection is a high-efficiency nucleic acid delivery system based on transferrin receptor-mediated endocytosis to carry DNA into cells. Furthermore it was shown that the cationic polymer polyethylenimine (PEI) mediates efficient gene transfer into a variety of cells. In the Polyethylenimine-Transferrinfection system the gene transfer efficiency of PEI/DNA complexes are combined with the specific mechanism of receptor-mediated endocytosis via Transferrin receptor.

The method results in a 30 - 1000-fold enhanced transfection efficiency depending on the cell line. It is an extremely gentle DNA transfection method whichemploys physiological uptake mechanisms of the cell. Transfection efficacy depends on the cell type, the level of surface transferrin receptor expression. Very high transfection rates have been shown for the tested tumor cell lines B16F10 melanoma, Neuro 2A neuroblastoma and a variety of primary human melanoma cell lines. In other established cell lines, such as HeLa, CHO, Jurkat, K562, HepG2 and COS the PEI-Transferrinfection works with high efficiences, excellent reproducibility and with the advantage of being an extremely gentle procedure.

Reactivity/Species
Human

Suitable Sample Types
Suspension cultures, Adherent cells

Reported Application
Cell Culture

Lipofectin™ Transfection Reagent Invitrogen™

Lipofectin® Transfection Reagent is the reagent of choice for transfection of endothelial cells. Lipofectin® Transfection Reagent is also suitable for transfecting DNA, RNA, and oligonucleotides into mammalian cells, and DNA into plant protoplasts. Lipofectin® reagent has also been shown to work well, in combination with PLUS® Reagent, for the transfection of HeLa cells. Lipofectin® Transfection Reagent is a 1:1 (w/w) liposome formulation of the cationic lipid N-[1-(2,3-dioleyloxy)propyl]-n,n,n-trimethylammonium chloride (DOTMA) and dioleoyl phophotidylethanolamine (DOPE) in membrane-filtered water.
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