Shop All Fluorescent in situ hybridization Reagents

View-miRNA 440µL Thermo Scientific™

View-miRNA 440µL

View-miRNA 1.44mL Thermo Scientific™

View-miRNA 1.44mL

View-miRNA 120µL Thermo Scientific™

View-miRNA 120µL

FISH Tag™ RNA Green Kit, with Alexa Fluor™ 488 dye Invitrogen™

FISH Tag™ RNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ RNA Kits in two Alexa Fluor® colors or get the FISH Tag™ RNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

ViewRNA™ ISH Cell 740 Module Invitrogen™

The ViewRNA ISH Cell 740 Module is designed to work in conjunction with both the ViewRNA ISH Cell Assay Kit (Cat. No. QVC0001) and ViewRNA Cell Plus Assay Kit (88-19000-99). It contains the signal amplification and AlexaFluor 750-based detection components for visualization of target mRNA using adherent or suspension cells. AlexaFluor 750 can be visualized with a Cy7 filter set, with excitation/emission maxima of 749/775 nm.

The QuantiGene ViewRNA ISH Cell 740 Module is supplied with enough reagents to process 24 assays when using the 24-well chamber plate assay format and 144 assays when using 96-well optical-bottom plates. The kit is configured for processing a minimum of 6-wells per experiment when using the 24-well plate assay format. If fewer assays are processed per experiment, reagent shortages may occur.

Reported applications:
Immunohistochemical staining

Note: We recommend the use of a strong antifade mountant, such as Prolong Glass Antifade Mountant (Cat. No. P36980), to minimize photobleaching.

AP Enhancer Solution Invitrogen™

AP Enhancer Solution, 10 mL is an aqueous buffered solution and an individual component of the ViewRNA™ ISH Tissue Assay Kits, 2-Plex, 24 assays. It is an enhancer solution for alkaline phosphatase.

FISH Tag™ DNA Green Kit, with Alexa Fluor™ 488 dye Invitrogen™

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

REMBRANDT™ Universal DISH & HRP (DIG-HRP) Detection Kit Thermo Scientific™

Specificity: ISH

Applications: ISH

ViewRNA™ Fast Red Buffer and Substrate Kit Invitrogen™

ViewRNA Fast Red Buffer and Substrate Kit is an individual component of the ViewRNA Chromogenic Signal Amplification Kit, 24 assays. It is a red substrate used for the detection of alkaline phosphatase activity.

FISH Tag™ RNA Red Kit, with Alexa Fluor™ 594 dye Invitrogen™

FISH Tag™ RNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ RNA Kits in two Alexa Fluor® colors or get the FISH Tag™ RNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 594 (590/615 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

100X Pretreatment Solution Invitrogen™

100X Pretreatment Solution is an aqueous buffered solution and an individual component of the ViewRNA™ ISH Tissue Assay Kits, 2-Plex, 24 assays.

ViewRNA 1440µL Thermo Scientific™

ViewRNA 1440µL

FISH Tag™ DNA Orange Kit, with Alexa Fluor™ 555 dye Invitrogen™

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 555 (555/565 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

AP Stop QT Invitrogen™

AP Stop QT, 10 mL, is an aqueous buffered solution intended for the inactivation of residual LP6-AP activity after the Fast Blue Substrate development. It is an individual component of the ViewRNA™ ISH Tissue Assay Kits, 2-Plex, 24 assays.
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