Cell Growth and Differentiation Reagents and Kits

Cell Growth and Differentiation Reagents and Kits

Cell growth and differentiation reagents and kits quantify viable cells and determine their differentiation and growth rates.
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Fluo-4 Direct™ Calcium Assay Kit Invitrogen™

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that: 1. Easily loads into cells 2. Achieves a large assay window and 3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the...

Fluo-4, AM, cell permeant Invitrogen™

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for...

FluoVolt™ Membrane Potential Kit Invitrogen™

The FluoVolt™ Membrane Potential Kit represents the next generation in voltage sensitive fluorescent probe-based kits. The FluoVolt™ probe brings together the best characteristics of the fast and slow response membrane potential fluorescent probes.

Fura-2, AM, FluoroPure™ grade - Special Packaging Invitrogen™

Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade Fura-2 AM is greater than or equal to 98% pure by HPLC. Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Fluo-4, Pentapotassium Salt, cell impermeant Invitrogen™

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-3 has been used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for...

Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit Invitrogen™

The Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit combines the ability to monitor the various stages of autophagy (through LC3B protein localization) with the high transduction efficiency and minimal toxicity of BacMam 2.0 expression technology.

Premo™ Autophagy Sensor LC3B-RFP (BacMam 2.0) Invitrogen™

Premo™ Autophagy Sensor combines the selectivity of a LC3B-red fluorescent protein (RFP) chimera with the transduction efficiency of BacMam technology, enabling unambiguous visualization of this protein.

Premo™ Autophagy Sensor LC3B-GFP (BacMam 2.0) Invitrogen™

Premo™ Autophagy Sensor combines the selectivity of a LC3B-green fluorescent protein (GFP) chimera with the transduction efficiency of BacMam technology, enabling unambiguous visualization of this protein.

BrdUTP (5-Bromo-2´-Deoxyuridine 5´-Triphosphate), 10 mM in TE buffer Invitrogen™

BrdUTP can be used in conjunction with tetrminal deoxynucleotidyl transferase (TdT) to label apoptotic cells in the TUNEL assay. BrdUTP can be detected with anti-BrdU antibodies.

BrdU (5-Bromo-2´-Deoxyuridine) Invitrogen™

Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.

BacMam GFP Transduction Control (BacMam 2.0) Invitrogen™

The BacMam 2.0 Transduction Control lets you experience the power and convenience of enhanced BacMam technology, with the convenient readout of emerald GFP (emGFP); our Aequorea victoria emGFP is extremely bright and photo stable and this non-targeted form is ideal for testing transduction.

CellMask™ Deep Red Plasma membrane Stain Invitrogen™

The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard...

Click-IT™ Palmitic Acid, Azide (15-Azidopentadecanoic Acid) Invitrogen™

Identify and characterize palmitylated proteins with Click-iT® palmitic acid, azide using the powerful click chemistry, a simple and robust two-step labeling and detection technique. In step one, the azide-containing biomolecule is fed to cells or animals and actively incorporated into proteins.

ATP Determination Kit, 200-1,000 assays Invitrogen™

Molecular Probes® ATP Determination Kit offers a convenient bioluminescence assay for quantitative determination of ATP with recombinant firefly luciferase and its substrate D-luciferin. The assay is based on luciferase’s absolute requirement for ATP in producing light (emission maximum ∼560 nm at...

Amplex™ Red Galactose/Galactose Oxidase Assay Kit Invitrogen™

The Amplex® Red Galactose/Galactose Oxidase Assay Kit provides a sensitive and simple method for detecting galactose oxidase activity or galactose using a fluorescence microplate reader or fluorometer. See our complete line of Fluorescence Microplate assays.

Click-iT™ EdU Imaging Kit with Alexa Fluor™ 488, 594, and 647 Azides Invitrogen™

The Click-iT® EdU Imaging Kit is a trial version of our superior alternative to traditional proliferation assays optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with...

Click-iT™ Cell Reaction Buffer Kit Invitrogen™

The Click-iT® Cell Buffer Kit provides researchers who have cells labeled with an azide or alkyne and the corresponding click detection reagent with the means to click these two moieties together. Each kit includes sufficient material to perform 50 reactions based upon a 0.

LIVE/DEAD™ Fixable Near IR (876) Viability Kit, for 808 nm excitation Invitrogen™

The LIVE/DEAD Fixable viability kits allow for clear determination of live and dead cells, enabling more precise analysis with both conventional and spectral flow cytometry. Cells labeled with LIVE/DEAD Fixable viability dyes can be washed, fixed, permeabilized, and stained for intracellular...

LIVE/DEAD™ Fixable Near IR (780) Viability Kit, for 633 nm excitation Invitrogen™

The LIVE/DEAD Fixable viability kits allow for clear determination of live and dead cells, enabling more precise analysis with both conventional and spectral flow cytometry. Cells labeled with LIVE/DEAD Fixable viability dyes can be washed, fixed, permeabilized, and stained for intracellular...

LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells Invitrogen™

The LIVE/DEAD® Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population based on plasma membrane integrity and esterase activity. The kit can be used in flow cytometry, fluorescence microscopy, and with fluorescence microplate readers.

LIVE BacLight™ Bacterial Gram Stain Kit, for microscopy & quantitative assays Invitrogen™

The LIVE BacLight™ Bacterial Gram Stain Kit provides a novel one-step fluorescence assay for the determination of gram sign in living bacteria. When gram negative and gram-positive bacterial are simultaneously stained with the membrane-permeant SYTO® 9 dye and hexidium iodide, gram-negative bacteria...

LIVE/DEAD™ Reduced Biohazard Cell Viability Kit #1, green & red fluorescence Invitrogen™

The LIVE/DEAD Reduced Biohazard Cell Viability Kit #1 is a convenient and easy-to-use cell viability kit that is designed to reduce the risk associated with handling potential biohazards such as viral, bacterial or protozoan pathogens.

LIVE/DEAD™ Fixable Orange (602) Viability Kit, for 561 nm excitation Invitrogen™

The LIVE/DEAD Fixable viability kits allow for clear determination of live and dead cells, enabling more precise analysis with both conventional and spectral flow cytometry. Cells labeled with LIVE/DEAD Fixable viability dyes can be washed, fixed, permeabilized, and stained for intracellular...

LIVE/DEAD™ Sperm Viability Kit Invitrogen™

The LIVE/DEAD Sperm Viability Kit provides a novel fluorescence-based assay for analyzing the viability and fertilizing potential of sperm by fluorescence microscopy or flow cytometry. Membrane-permeant SYBR® 14 nucleic acid stain labels live sperm with green fluorescence, and membrane-impermeant...

LDS 751 Invitrogen™

LDS 751 is a cell-permeant nucleic acid stain that has a peak excitation at ∼543 nm on dsDNA and is particular useful in multicolor analyses due to its long wavelength emission maximum (∼712 nm). Binding of LDS 751 to dsDNA results in a ∼20-fold fluorescence enhancement.

HCS CellMask™ Deep Red Stain Invitrogen™

HCS CellMask™ Deep Red stain (H32721) is a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell and provide an accurate backdrop against which the features of interest can be assessed.

HCS CellMask™ Green Stain Invitrogen™

HCS CellMask™ Green stain (H32714) is a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (nucleus and cytoplasm) and provide an accurate backdrop against which the features of interest can be assessed.

BODIPY™ FL GDP (Guanosine 5'-Diphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Bis (Triethylammonium) Salt) Invitrogen™

The mixed-isomer analog of BODIPY® FL GDP is comprised of the BODIPY® FL fluorophore attached to the 2’ or 3’ position of the ribose ring via an aminoethylcarbamoyl linker. BODIPY® FL exhibits spectral characteristics similar to fluorescein (FITC) or the Alexa Fluor® 488 dye.

HCS LipidTOX™ Red Phospholipidosis Detection Reagent (1000X), for cellular imaging Invitrogen™

The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect...

Indo-1, AM, cell permeant Invitrogen™

Indo-1, AM is a high affinity, intracellular calcium indicator (Kd ∼0.23 µM) that is ratiometric and UV light—excitable. This acetoxymethyl (AM) ester form is useful for noninvasive intracellular loading and is also available in special packaging (I-1223) and in a DMSO solution (I-1226).

HCS Mitochondrial Health Kit Invitrogen™

The HCS Mitochondrial Health Kit simultaneously and quantitatively measures two important cell-health parameters: mitotoxicity and cytotoxicity. The MitoHealth stain detects changes in the mitochondrial membrane potential, whereupon the reagent accumulates in active mitochondria.

HCS CellMask™ Orange Stain Invitrogen™

HCS CellMask™ Orange stain (H32713) is a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell and provide an accurate backdrop against which the features of interest can be assessed.

HCS LipidTOX™ Green Phospholipidosis Detection Reagent (1000X), for cellular imaging Invitrogen™

The HCS LipidTOX™ Green phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect...

HCS NuclearMask™ Red Stain Invitrogen™

The versatile HCS NuclearMask™ Red stain (H10326) can be used to measure DNA content and perform cell demarcation with formaldehyde-fixed cells on high content imaging and analysis (HCS) platforms. With excitation⁄emission maxima of 622⁄645 nm when bound to DNA, it is easily discriminated from green...

HCS DNA Damage Kit Invitrogen™

The HCS DNA Damage kit simultaneously and quantitatively measures two important cell-health parameters: DNA damage and cytotoxicity. DNA damage is detected using an antibody against phosphorylated H2AX (Ser139) which is induced in response to double-strand break (DSB) formation.

EnzChek™ Phosphatase Assay Kit Invitrogen™

Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes' patented 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate (D6567).

EnzChek™ Gelatinase/Collagenase Assay Kit, 250-2,000 assays Invitrogen™

The EnzChek Gelatinase/Collagenase Assay Kit provides a sensitive, convenient and fast fluorometric method for measuring gelatinase or collagenase activity in purified enzyme systems, cell/tissue lysates or for screening inhibitors in a high-throughput format.

EnzChek™ Reverse Transcriptase Assay Kit Invitrogen™

The EnzChek® Reverse Transcriptase Assay Kit is a convenient, efficient, and inexpensive assay for measuring reverse transcriptase activity. In less than an hour, samples can be read in a fluorometer or microplate reader with filter sets appropriate for fluorescein (FITC).

EnzChek™ Phospholipase A1 Assay Kit Invitrogen™

This kits takes our stand-alone assay for phospholipase A1, PLA1 (A10070) and combines the necessary reagents to run 2 to 10 complete 96 well microplate assays that monitor activity in purified enzyme preparations and cell lysates.

EnzChek™ Ultra Amylase Assay Kit Invitrogen™

The EnzChek® Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format.

EnzChek™ 3C-like Protease (3CLpro) & Papain-like Protease (PLpro) Assay Kits Invitrogen™

Easily and quickly quantify inhibitors of SARS-CoV-2 3C-like protease (3CLpro) and papain-like protease (PLpro) using the EnzChek 3CLpro and PLpro Assay kits. These kits can be used to screen and profile inhibitors of 3CLpro and PLpro and include all reagents for the determination of protease...

EnzChek™ Pyrophosphate Assay Kit Invitrogen™

The EnzChek® Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. In this assay the formation of a chromophoric product is detected spectrophotometrically. See our complete line of Fluorescence Microplate assays.

Guanosine 5'-Triphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Trisodium Salt (BODIPY™ FL GTP) Invitrogen™

BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of...

Cascade Blue™ hydrazide, Trilithium Salt Invitrogen™

The cell-impermeant, fixable, polar tracer Cascade Blue® hydrazide can be used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.

CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry Invitrogen™

CellTrace™ Far Red Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. • Superior performance—bright, single-peak staining enables visualization of multiple generations • Long-term signal...

CellTrace™ Calcein Blue, AM - Special Packaging Invitrogen™

CellTrace calcein blue AM is a proven short-term, blue-fluorescent tracer for cell labeling. The probe possesses acetoxymethyl (AM) esters that allow its passive diffusion across cell membranes. Upon cleavage of the AM esters by intracellular esterases, the molecule that remains (absorption/emission...

Click-iT™ EdU Proliferation Assay for Microplates Invitrogen™

The Click-iT EdU Proliferation Assay for Microplates provides a more simplified and robust method for the measurement of mammalian cell proliferation compared to traditional BrdU-based methods. After incorporation of EdU (a modified thymidine analogue) into newly synthesize DNA, horse radish...

CyQUANT™ LDH Cytotoxicity Assay, fluorescence Invitrogen™

The CyQUANT LDH Cytotoxicity Assay, fluorescence, is a fluorescent assay that provides a simple and reliable method for determining cellular cytotoxicity. Lactate dehydrogenase (LDH) is a cytosolic enzyme present in many different cell types that is released into the cell culture medium upon damage...

Amplex™ Red Glucose/Glucose Oxidase Assay Kit Invitrogen™

The Amplex® Red Glucose/Glucose Oxidase Assay Kit provides a sensitive and simple method for detecting d-glucose or glucose oxidase using a fluorescence microplate reader or fluorometer. See our complete line of Fluorescence Microplate assays. • Detect concentrations as low as 3 µM d-glucose or 0.

Alexa Fluor™ 647 ATP (Adenosine 5'-Triphosphate, Alexa Fluor™ 647 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Hexa(Triethylammonium) Salt) Invitrogen™

Alexa Fluor® 647 adenosine 5´triphosphate (Alexa Fluor® 647 ATP) is a mixed-isomer comprised of the Alexa Fluor® 647 fluorophore, an exceptionally bright far-red fluorescent dye attached to the 2´ or 3´ position of the ribose ring via an aminoethylcarbamoyl linker.

alamarBlue™ HS Cell Viability Reagent Invitrogen™

alamarBlue HS Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability.

CellMask™ Green Actin Tracking Stain Invitrogen™

Invitrogen CellMask Green Actin Tracking Stain fluorescently labels polymerized/filamentous actin (F-actin) in live or fixed cells. It is designed to readily permeate live cells, thus providing more uniform and bright labeling.

Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye Invitrogen™

The Click-iT EdU Cell Proliferation Kit for Imaging is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently...

Click-IT™ Geranylgeranyl Alcohol, Azide, mixed isomers Invitrogen™

Identify and characterize geranylated proteins with Click-iT® geranylgeranyl alcohol, azide using the powerful click chemistry, a simple and robust two-step labeling and detection technique. In step one, the azide-containing biomolecule is fed to cells or animals and actively incorporated into...

Click-iT™ Protein Reaction Buffer Kit Invitrogen™

The Click-iT® Protein Buffer Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click detection reagent with the means to click these two moieties together.

Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay Invitrogen™

The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging.

Click-iT™ EdU Alexa Fluor™ 594 HCS Assay Invitrogen™

The Click-iT® EdU Alexa Fluor® 594 HCS Assay is a superior alternative to traditional proliferation assays that is optimized for high-content imaging applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with...

Click-iT™ Protein Enrichment Kit, for click chemistry capture of azide-modified proteins Invitrogen™

The Click-iT® Protein Enrichment Kit for the efficient capture of click chemistry based azide-modified proteins on a resin of alkyne agarose. Superior to biotin or lectin based enrichment approaches. Ideal for proteomics, biomarker discovery, posttranslational modification (PTM) analysis, and more.

Click-IT™ GalNAz Metabolic Glycoprotein Labeling Reagent (Tetraacetylated N-Azidoacetylgalactosamine) Invitrogen™

The Click-iT® GalNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize cell surface O-linked glycoproteins. In step one, cultured cells are incubated with the azide-modified galactosamine (GalNAz).

Click-IT™ Biotin Protein Analysis Detection Kit Invitrogen™

The Click-iT® Biotin Glycoprotein Detection Kit provides the second part of a simple and robust technique to identify and characterize glycoproteins by Western blot. In step two, after the incorporation of an azide moiety into protein glycan structures with either a Click-iT® metabolic labeling...
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