Shop All Cell Growth and Differentiation Reagents and Kits

Product Type

StemPro™ Adipogenesis Differentiation Kit Gibco™

For Complete Differentiation of Human Mesenchymal Stem Cells into Fat Cells

StemPro® Adipogenesis Differentiation Kit has been developed for the adipogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the adipogenesis pathway and generate adipocytes. Using StemPro® Adipogenesis Differentiation Kit in combination with StemPro® MSC SFM or MesenPRO RS™ Medium provides a standardized culture workflow solution for MSC isolation, expansion and differentiation into lipid vesicle-forming adipocytes.

CyQUANT™ NF Cell Proliferation Assay Invitrogen™

The CyQUANT® NF Cell Proliferation Assay provides a fast and sensitive method for counting cells in a population and measuring proliferation in microplate format.

Features of the CyQUANT® NF Cell Proliferation Assay:

• More sensitive than MTT or AlamarBlue® assays
• Linear detection range from 100 to 20,000 cells per well (96-well microplate)
• Assays can be completed in one hour

A Rapid and simple method for measuring cell proliferation
The CyQUANT® NF Cell Proliferation Assay does not require cell lysis, long incubations, radioactivity, or removal of stain from cells. The CyQUANT® NF assay eliminates the freeze-thaw cell lysis step of the original CyQUANT® cell proliferation assay by using a cell-permeant DNA-binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT® NF Cell Proliferation Assay can be used in either 96-well or 384-well microplate formats, and is available in two configurations: a 200 assay kit (C35007) for smaller sample sizes, and a 1000 assay kit (C35006) for high-throughput applications.

AlamarBlue® is a registered trademark of TREK Diagnostic Systems.

StemPro™ Chondrogenesis Differentiation Kit Gibco™

The StemPro® Chondrogenesis Differentiation Kit has been developed for the chondrogenic differentiation of mesenchymal stem cells (MSCs) in tissue-culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the chondrogenesis pathway and generate chondrocytes. Using StemPro® Chondrogenesis Differentiation Kit in combination with StemPro® MSC SFM or MesenPRO RS™ Medium provides a standardized culture workflow solution for MSC isolation, expansion, and differentiation into collagen matrix-producing chondrocytes.

CyQUANT™ Direct Cell Proliferation Assay Invitrogen™

Experience a more convenient workflow for your high-throughput cytotoxicity and proliferation screening projects with the CyQUANT® Direct Cell Proliferation Assay–no washes, cell lysis, temperature equilibrations, or radioactivity required. DNA-based assays have been shown to be among the most sensitive cell health indicators and data concordance with metabolically based assays is excellent. The CyQUANT® Direct assay is a highly sensitive and robust method to assess cell growth, viability, or compound toxicity in a range of applications, from high throughput screening to bioproduction.

Features of the CyQUANT® Direct Cell Proliferation Assay include:

• More convenient workflow for high-throughput screening–no washes, cell lysis, temperature equilibrations, or radioactivity required
• Rapid protocol, high sensitivity and dynamic range
• Measures independent of metabolic state of cells on standard fluorescent plate readers
• Multiplex assays using a spectrally distinct fluorescent or a luminescent readout

Rapid and convenient, with a large dynamic range
The CyQUANT® Direct assay is a fluorescence-based proliferation and cytotoxicity assay for microplate readers with a linear detection range from less than 100 to 20,000 cells per well in most cell types. The no-wash, homogenous format and fast add-mix-read protocol makes the CyQUANT® Direct assay ideal for HTS applications. The assay can be completed in one hour, with no washes, no cell lysis, or temperature equilibrations required. The signal is stable for several hours, affording additional work flow convenience.

How it works
The CyQUANT® Direct assay is based on a cell-permeant DNA-binding dye in combination with a masking dye reagent. Cellular DNA is highly regulated and estimates of cell numbers based on DNA are very accurate. The masking dye blocks staining of dead cells or cells with compromised cell membranes resulting in only healthy cells being stained. The CyQUANT® Direct assay, therefore, measures proliferation as well as membrane integrity, another measure of cell health. Users of metabolically based cell health assays can readily compare historical data with results from the CyQUANT® Direct assay, as concordance assays based on metabolism or energy (ATP) has been shown to be excellent. Furthermore, it is possible to multiplex with spectrally distinct fluorescent probes or luminescence assay kits because cells are not lysed. Furthermore, the fluorescent signal allows the user to switch between HTS and HCS plate readers if multiplex assays require different platforms.

StemPro™ Osteogenesis Differentiation Kit Gibco™

For Complete Differentiation of Human Mesenchymal Stem Cells into Bone Cells

StemPro® Osteogenesis Differentiation Kit has been developed for the osteogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the osteogenesis pathway and generate
immature and mature osteocytes. Using StemPro® Osteogenesis Differentiation Kit in combination with MesenPRO RS™ Medium or StemPro® MSC SFM provides a standardized culture workflow solution for MSC isolation, expansion and differentiation into mineralized matrix-producing osteocytes.

alamarBlue™ Cell Viability Reagent Invitrogen™

alamarBlue Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Resazurin, the active ingredient of alamarBlue reagent, is a non-toxic, cell-permeable compound that is blue in color and virtually non-fluorescent. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent. Changes in viability can be easily detected using either an absorbance- or fluorescence-based plate reader. alamarBlue Cell Viability Reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi.

Features include:
• Robust and reliable performance—results in a large, highly reproducible dynamic range
• Highly sensitive reagent with a linear response—detects as few as 50 cells per well
• Convenient add-and-read format—no mixing, no washing, no cell lysis
• Compatible with either fluorescence- or absorbance-based instrumentation
• Measures viability from many diverse cell types—including mammalian cells, bacteria, plant, and fungi

Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and cellular reducing conditions are known indicators of cell viability and death. alamarBlue Cell Viability Reagent is an indigo-colored, non-toxic reagent that detects metabolically active cells and is used for the quantitative analysis of cell viability and proliferation. alamarBlue reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi.

When added to cells, alamarBlue Cell Viability Reagent is modified by the reducing environment of viable cells and turns red in color and becomes highly fluorescent. This color change and increased fluorescence can be detected using absorbance (detected at 570 and 600 nm) or fluorescence (using an excitation between 530–560 and an emission at 590 nm). To assay for viability, simply add the pre-mixed alamarBlue reagent to cells in complete media (no wash or cell lysis steps required), incubate for one to four hours, and read using either an absorbance- or fluorescence-based plate reader. If necessary, longer incubation times may be used for greater sensitivity without compromising cell health.

The homogeneous, add-and-read assay format of the alamarBlue Cell Viability Reagent is fast, convenient, and readily amenable to automation and high-throughput assays. The color change and fluorescence increase resulting from cell viability changes allow the detection of alamarBlue reagent by either an absorbance- or fluorescence-based instrumentation.

EnzChek™ Ultra Xylanase Assay Kit Invitrogen™

The EnzChek® Ultra Xylanase Assay Kit features a quick and convenient mix-and-read format for the detection and monitoring of xylanase activity. The increase in fluorescence resulting from xylanase activity is measured using a fluorometer or fluorescence microplate reader.

See our complete line of Fluorescence Microplate assays.

• Detect concentrations of xylanase activity as low as 1.5 mU/mL
• Suitable for a broad pH range (pH 4 to 10)
• Format allows for continuous detection of xylanase activity
• Excitation/emission maxima of ~358/455 nm, well suited for DAPI filter settings

The EnzChek® Ultra Xylanase Assay Kit provides the speed, sensitivity, and convenience required for measuring xylanase activity or for screening xylanase inhibitors in a high-throughput format. The hydrolysis of xylosidic linkages within the included Xylanase Substrate (hemicellulose polysaccharides) results in the unquenching of the attached fluorescent dyes. This kit can be used for continuous detection of xylanase activity, and offers broad dynamic and pH ranges. Each kit contains sufficient substrate for ~500 assays in a 96-well microplate format. Additionally, the kit contains a fluorescent reference standard that can be used to quantify the xylanase activity.

Nα-(3-Maleimidylpropionyl)Biocytin Invitrogen™

The thiol-reactive Na-(3-maleimidylpropionyl) biocytin can be used to attach biotin to biomolecules that can be subsequently detected with avidin or streptavidin conjugates.

DMNPE-caged ATP (Adenosine 5'-Triphosphate, P3-(1-(4,5-Dimethoxy-2-Nitrophenyl)ethyl) Ester, Disodium Salt) Invitrogen™

DMNPE-caged ATP is a nucleotide analog in which the terminal phosphate is esterified with a blocking group, rendering the molecule biologically inactive. Flash photolysis of the caging group with UV illumination at 355 nm results in a rapid and highly localized release of the free nucleotide at the site of illumination. The DMNPE caging group absorbs light more efficiently than CNB and NPE caging groups at ~360 nm, but photolysis rates and quantum yields are generally lower.

LIVE/DEAD™ Fixable Red Dead Cell Stain Kit, for 488 nm excitation Invitrogen™

The LIVE/DEAD™ Fixable Red Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a blue laser flow cytometer.

• Stable—dyes are freeze dried in separate vials to maintain stability

• Robust—staining pattern is the same before and after fixation

• Bright signal—allows for easy distinction between live/dead cells in single channel

View a selection guide for all fixable viability dyes for flow cytometry.

Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Red Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Optimal brightness
The LIVE/DEAD™ Fixable Red stain was selected based on its fluorescent properties to provide a bright signal when excited with a yellow, green, or blue 488 nm laser. The red-fluorescent reactive dye has an excitation maximum of ~595 nm, making it ideal for use with the yellow laser, and an emission of ~615 nm, allowing collection in the 610 or 630 BP filter. LIVE/DEAD™ Fixable Red Dead Cell stain is also excited well with the blue 488 nm laser. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.

How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available
LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.

Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye Invitrogen™

The Click-iT EdU Cell Proliferation Kit for Imaging is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor® dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.

• Simple—works the first time, every time, in less time
• Efficient—no denaturation steps or harsh treatment required
• Content-rich results—better preservation of cell morphology, antigen structure, and DNA integrity
• Consistent—not dependent on variable antibody lots for detection

The most accurate proliferation-detection methods are based on the incorporation and measurement of nucleoside analogues in newly synthesized DNA, with bromodeoxyuridine (BrdU) a commonly used analogue. BrdU-labeled DNA is quantitated using anti-BrdU antibodies following DNA denaturation by harsh methods (HCl, heat, or enzymes) to expose the BrdU molecules. This step is time consuming and difficult to perform consistently. The harsh treatment can also adversely effect sample integrity and quality, which makes co-staining with other antibodies challenging.

Superior Proliferation Methodology
The Click-iT EdU Cell Proliferation Kit for Imaging provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT® EdU, mild fixation and detergent permeabilization is sufficient for the small molecule-based Click-iT® EdU detection reagent to gain access to the DNA. As a consequence, the Click-iT® EdU imaging kit is not only easy to use, but more accurate and compatible with cell cycle analysis and other intracellular or extracellular targets for truly content-rich results.

This kit is optimized for fluorescence microscopy applications; visit the Click-iT® technology area of our website for kits designed for high-content imaging, fluorescence microplate, or flow cytometry platforms.

Learn more about Click-iT technology >
Find more tools for image-based detection of proliferating cells >

Notes:
The Click-iT® assay can be used on cells in culture or in vivo following EdU administration by feeding or injection methods.
The Click-iT® assay can be used with BrdU in dual pulse experiments by using the anti-BrdU (clone MoBu-1) antibody, which does not cross react with EdU.
The Click-iT® technology is compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed cell labeling.

Amplex™ Red/UltraRed Stop Reagent Invitrogen™

Amplex® Red/UltraRed Stop Reagent is designed for use with the Amplex® Red and Amplex® UltraRed fluorogenic substrates and assay kits. Amplex® Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least 3 hours.

See our complete line of Fluorescence Microplate assays.

Amplex® UltraRed and Amplex® Red assay kits are sensitive biomolecular assays based on hydrogen peroxide–generating enzyme systems linked to peroxidase–mediated oxidation of the fluorogenic Amplex® UltraRed or Amplex® Red substrates. Typically, detection reactions are performed in microplate wells and are initiated by adding the fluorogenic Amplex® UltraRed or Amplex® Red substrate, resulting in a continuous fluorescence increase that proceeds for 30 minutes or more. Ultimately, unknown analyte concentrations are determined by comparing fluorescence intensities measured at a certain time point during the reaction to parallel measurements at the same time point on standard samples of known concentration. Clearly, it is critical to ensure that the timing of the standard and unknown sample measurements is the same.

Amplex® Red/UltraRed Stop Reagent is designed for use with both the Amplex® Red and Amplex® UltraRed fluorogenic substrates and assay kits. It is designed to terminate reactions containing up to 0.1 units/mL of horseradish peroxidase (HRP) and 5 μM hyrogen peroxide. The fluorescent signal then remains stable for at least 3 hours.

EnzChek™ Ultra Amylase Assay Kit Invitrogen™

The EnzChek® Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek® kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY® FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

EnzChek® Ultra Amylase Assay Kit Specifications:
• Label (Ex/Em): BODIPY® FL conjugate (~502/512 nm)
• Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
• Sufficient reagents are supplied for 500 assays (using a 100 µL assay volume in a 96-well microplate assay format)


Find Fluorescent Substrates for Other Glycosidases
In addition to the EnzChek® Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more. Review Detecting Glycosidases—Section 10.2 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

CyQUANT™ Direct Red Cell Proliferation Assay Invitrogen™

The CyQUANT Direct Red Cell Proliferation Assay is a simple no-wash, add-and-read fluorescent microplate assay that can be used to assess cell growth, viability, or compound toxicity. The assay consists of a cell-permeant DNA-binding red fluorescent probe and a cell-impermeant background suppressor. The probe and suppressor are mixed and added to cells; the probe stains all cells, while the suppressor only enters dead cells or cells with compromised cell membranes, suppressing probe fluorescence in those locations. The result is that only healthy cells are detected.

CyQUANT Direct Red Cell Proliferation Assays features include:
Convenient—no washes, cell lysis, or temperature equilibration; simply add and read to detect changes in adherent and suspension cell proliferation
Accurate―based on amount of cellular DNA, which is highly regulated and independent of the metabolic state of the cells
Robust―optimized red fluorescent probe and background suppressor result in a highly sensitive assay with a large dynamic range
Stable―red fluorescent signal stability for seven hours, ideal for large screening assays
Multiplex enabled―can be combined with spectrally distinct fluorescent or luminescent readouts

The CyQUANT Direct assay is a red fluorescence-based proliferation and cytotoxicity assay for microplate readers with a linear detection range from less than 100 to 20,000 cells per well for most cell types. The no-wash, add-mix-read assay can be completed in one hour. The red fluorescent excitation and emission wavelengths result in an assay that is ideal for multiplexing with green fluorescent dyes or proteins. Additionally, the fluorescent signal is stable for several hours, for additional workflow convenience.

Since the amount of DNA in cells is highly regulated, detection methods based on determining the amount of DNA are highly accurate. The CyQUANT Direct Red Cell Proliferation Assay consists of a red fluorescent cell-permeant DNA-binding probe and a background suppressor dye. The probe and suppressor are mixed, added to cells, incubated for one hour, and then the fluorescence measured. The probe enters all cells, but the suppressor only enters dead cells or cells with compromised membranes. Upon entering dead and dying cells the background suppressor dye suppresses the signal from the fluorescent probe, resulting in the detection of only healthy cells. Thus, the CyQUANT Direct Red assay can be used to measure changes in proliferation as well as cytotoxicity.

Fluo-4 NW Calcium Assay Kit Invitrogen™

Fluo-4 NW (No-Wash) Calcium Assay Kitsoffer a proprietary calcium assay formulation that requires neither a washstep nor a quencher dye. The fluo-4 NW assay achieves largerincreases in fluorescence intensity than standard fluo-3 and fluo-4assays with a wash step. Eliminating the wash step results inlower variability and higher Z´ values than the standard fluo-4 assay,while providing an easier and faster assay as well.

The fluo-4NW indicator is nonfluorescent and stable in pH 7–7.5 buffer forseveral hours, so spontaneous conversion to the Ca2+-sensitive formis not a significant source of background fluorescence. Contributionsto baseline fluorescence by the growth medium (e.g., esteraseactivity, proteins interacting with receptors of interest, or phenolred) are eliminated by removing the medium prior to adding theindicator dye to the wells.

Another source of potential fluorescenceoutside the cells is extrusion of the indicator out of the cell by organicanion transporters. Probenecid is commonly used to inhibitthis transport and reduce the baseline signal. We have synthesizeda proprietary water-soluble probenecid, which is supplied with theFluo-4 NW Calcium Assay Kits. This form of probenecid has theadvantages of being easy to dissolve in buffer and safer to use thanthe free acid, which requires caustic 1 M NaOH to dissolve. TheFluo-4 NW Calcium Assay Kits are designed for microplates andHTS, and the assay can be performed on adherent as well as nonadherentcells.

Fluo-4 AM is a fluorescent Ca+2 indicator that is widely usedfor in-cell measurement of agonist-stimulated and antagonist inhibitedcalcium signaling in high-throughput screening (HTS)applications. Its visible wavelength excitation (compatible withargon-ion laser sources), high sensitivity, and large fluorescenceincrease upon binding Ca2+ has made it the indicator of choicefor characterizing G-protein–coupled receptor (GPCR) pharmacologyand function. These properties have made fluo-4 AMattractive not only for microplate screening applications but formicroscopy and flow cytometry as well.

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