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Dextran, Fluorescein and Biotin, 3000 MW, Anionic, Lysine Fixable (Micro-Emerald) Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Fluorescein & Biotin (494/518)
Size: 3,000 MW
Charge: Anionic
Fixable: Fixable via Lysine

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Lucifer Yellow CH, Lithium Salt, 25 mg Invitrogen™

Lucifer yellow CH, lithium salt is a water-soluble dye with excitation/emission peaks of 428/536 nm. It is a favorite tool for studying neuronal morphology, because it contains a carbohydrazide (CH) group that allows it to be covalently linked to surrounding biomolecules during aldehyde fixation. The lithium salt form of the lucifer yellow CH is commonly used for microinjection because it has higher solubility than the potassium and ammonium salt forms of lucifer yellow CH.

NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each Invitrogen™

NeuroTrace® Multicolor Tissue-Labeling Kit contains 500 mg each of DiI, DiO or DiD mixed into an inert, water-resistant gel. The paste is ready to use as supplied and can be applied directly to live or fixed tissue specimens using the tip of a needle. This method of application improves the penetration of the dye into bundled neurons, labeling axons both on and below the surface.

Dextran, Texas Red™, 10,000 MW, Neutral Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Texas Red® (595/615)
Size: 10,000 MW
Charge: Zwitterionic
Fixable: Nonfixable

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Dextran, Tetramethylrhodamine, 40,000 MW, Neutral Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Tetramethylrhodamine (555/580)
Size: 40,000 MW
Charge: Anionic
Fixable: Nonfixable

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

FluoroMyelin™ Green Fluorescent Myelin Stain - Solution in Water Invitrogen™

The FluoroMyelin™ Green fluorescent myelin stain enables quick and selective labeling of myelin in brain cryosections in a single 20-minute labeling step plus washes. This stain can be used in conjunction with antibodies and other dyes, and with standard histochemical methods for cryosection material.

Dextran, Alexa Fluor™ 488; 3,000 MW, Anionic Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Alexa Fluor® 488 (495/519)
Size: 3,000 MW
Charge: Anionic
Fixable: Nonfixable

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Dextran, Tetramethylrhodamine and biotin, 3000 MW, Lysine Fixable (micro-Ruby) Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Tetramethylrhodamine & Biotin (555/580)
Size: 3,000 MW
Charge: Anionic
Fixable: Fixable via Lysine

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

RH 237 (N-(4-Sulfobutyl)-4-(6-(4-(Dibutylamino)phenyl)hexatrienyl)Pyridinium, Inner Salt) Invitrogen™

The fast-response potential-sensitive probe RH 237 can be used for imaging functional neurons.

Dextran, Amino, 70,000 MW Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): None (can be conjugated to dyes and other molecules using amine-reactive moities)
Size: 70,000 MW

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Dextran, Fluorescein, 2,000,000 MW, Anionic, Lysine Fixable Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Fluorescein (494/521)
Size: 2,000,000 MW
Charge: Anionic
Fixable: Fixable via Lysine

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Dextran, Texas Red™, 40,000 MW, Neutral Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Texas Red® (595/615)
Size: 40,000 MW
Charge: Zwitterionic
Fixable: Nonfixable

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Dextran, Alexa Fluor™ 555; 10,000 MW, Anionic, Fixable Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Alexa Fluor® 555 (555/565)
Size: 10,000 MW
Charge: Anionic
Fixable: Fixable via free amines

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Dextran, Fluorescein, 40,000 MW, Anionic Invitrogen™

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Fluorescein (494/521)
Size: 40,000 MW
Charge: Anionic
Fixable: Nonfixable

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

FluoroMyelin™ Red Fluorescent Myelin Stain - Solution in Water Invitrogen™

The FluoroMyelin™ Red fluorescent myelin stain enables quick and selective labeling of myelin in brain cryosections in a single 20-minute labeling step plus washes. This stain can be used in conjunction with antibodies and other dyes, and with standard histochemical methods for cryosection material.
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