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SURF-CAL™ Particle Size Standards Thermo Scientific™
Simplify the preparation of calibration wafers with Thermo Scientific™ SURF-CAL™ Particle Size Standards, for calibration and maintenance of Scanning Surface Inspection Systems (SSIS). Available particle sizes correspond to the calibration point sizes required by instrument manufacturers.
Exosome-Human CD9 Isolation Reagent (from cell culture) Invitrogen™
Exosome-Human CD9 Isolation Reagent (from cell culture) enables isolation of CD9-positive extracellular vesicles (ECVs) such as exosomes from enriched cell culture media. Following isolation, the specific sub-population of exosomes/ECVs can be further studied using methods like Western blotting, qPCR, or next generation sequencing.
• Minimal hands-on time for specific exosome isolation
• Obtain a highly pure exosome subset
• Protocol is easily scalable
• Compatible with most downstream analysis
Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.
Dynabeads® magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads® magnetic separation technology also allows you to 'see' your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.
Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.
• Minimal hands-on time for specific exosome isolation
• Obtain a highly pure exosome subset
• Protocol is easily scalable
• Compatible with most downstream analysis
Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.
Dynabeads® magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads® magnetic separation technology also allows you to 'see' your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.
Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.
4000 Series Monosized Particles Thermo Scientific™
Minimize response of analytic systems to shape effects with Thermo Scientific™ 4000 Series Monosized Particles, excellent for use with any application that requires a NIST™ traceable size standard with a very narrow standard deviation.
3000 Series Nanosphere™ Size Standards Thermo Scientific™
Conveniently check the sizes of bacteria, viruses, ribosomes and sub-cellular components with Thermo Scientific™ 3000 Series Nanosphere™ Size Standards, excellent for use with any application that requires a NIST™ traceable size standard with a very narrow standard deviation. The 20 to 900nm range of spheres is ideal for the calibration of electron and atomic force microscopes. Standards are also used in laser light scattering studies and colloidal systems research.
Total Exosome Isolation Kit (from plasma) Invitrogen™
The Total Exosome Isolation Kit (from plasma) enables fast and efficient enrichment of intact exosomes from plasma samples. The kit consists of two parts: Total Exosome Isolation Reagent (from plasma) and Proteinase K (20 mg/mL). The Total Exosome Isolation Reagent enables simple and reliable concentration of intact exosomes from plasma samples with a protocol that is scalable depending on your sample size. Proteinase K is provided separately for optional upfront plasma treatment and is used to eliminate all plasma protein and thus maximize the purity of the exosome preparation.
• Maximize recovery of intact exosomes for any type of downstream application
• Easily isolate exosomes using our simple and reliable protocol
• Avoid time-consuming ultra-centrifugation
• Maximize flexibility—scale up or down depending on the sample size
By tying up water molecules, the Total Exosome Isolation Reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. After a 10 minute treatment with Proteinase K, the reagent is added to the plasma and the solution is incubated for 30 min at 2–8°C. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 5 min at room temperature. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can then be purified using the Total Exosome RNA and Protein Isolation Kit (Cat# 4478545), or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking.
For downstream mass spectrometry analysis we recommend performing gel electrophoresis purification of the isolated exosome suspension prior to analysis.
• Maximize recovery of intact exosomes for any type of downstream application
• Easily isolate exosomes using our simple and reliable protocol
• Avoid time-consuming ultra-centrifugation
• Maximize flexibility—scale up or down depending on the sample size
By tying up water molecules, the Total Exosome Isolation Reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. After a 10 minute treatment with Proteinase K, the reagent is added to the plasma and the solution is incubated for 30 min at 2–8°C. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 5 min at room temperature. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can then be purified using the Total Exosome RNA and Protein Isolation Kit (Cat# 4478545), or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking.
For downstream mass spectrometry analysis we recommend performing gel electrophoresis purification of the isolated exosome suspension prior to analysis.
9000 Series Glass Particle Standards Thermo Scientific™
Replace polystyrene spheres in many applications with Thermo Scientific™ 9000 Series Glass Particle Standards, dry glass NIST™-traceable size standards. Glass microspheres can be used in any application that requires a NIST™-traceable size standard with a narrow size distribution where sample conditions may not be suitable for polystyrene spheres. The glass microspheres provide a better tolerance to chemicals and solvents in addition to higher mechanical and thermal stability.
Dynabeads™ Untouched™ Mouse T Cells Kit Invitrogen™
Dynabeads® Untouched™ Mouse T Cells contains both magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse T cells. The negatively isolated, untouched mouse T-cells are pure, viable and ready to be used in any downstream application.
• Rapid isolation of untouched mouse T-cells – no columns required
• Pure and viable mouse T-cells ready for any downstream application
Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that bind mouse B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-T cells. The cells are washed to remove excess antibodies, and Mouse Depletion Dynabeads® are then added. After a short incubation, the bead-bound cells are separated within 1 – 2 minutes using a magnet. The purified target mouse T-cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched T cells. The protocol can easily be scaled up according to your sample size.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
• Rapid isolation of untouched mouse T-cells – no columns required
• Pure and viable mouse T-cells ready for any downstream application
Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that bind mouse B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-T cells. The cells are washed to remove excess antibodies, and Mouse Depletion Dynabeads® are then added. After a short incubation, the bead-bound cells are separated within 1 – 2 minutes using a magnet. The purified target mouse T-cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched T cells. The protocol can easily be scaled up according to your sample size.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Aliphatic Amine Latex Beads, 2% w/v, 0.4 µm Invitrogen™
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.
This aliphatic amine latex has a high density of amine groups attached to the terminus of an aliphatic six-carbon spacer arm. This product can be used to covalently couple proteins. Location of the amine at the end of the spacer arm minimizes difficulties with steric hindrance and conformation since the group has a greater freedom to rotate. In addition, spacer arms improve the kinetics of latex agglutination reactions. The particles are stabilized by the positively-charged amine groups under low to neutral pH conditions. Care should be taken not to use them under high pH.
Microspheres Are Available in Many Sizes and Surface Functionalities
• 25 sizes to choose from—0.02 microns to 16 microns
• 20 different surface—for ready conjugation to virtually any material
• Surfactant free—ultraclean
• Various quantities—from milliliter to 500-liter amounts
Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.
We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
This aliphatic amine latex has a high density of amine groups attached to the terminus of an aliphatic six-carbon spacer arm. This product can be used to covalently couple proteins. Location of the amine at the end of the spacer arm minimizes difficulties with steric hindrance and conformation since the group has a greater freedom to rotate. In addition, spacer arms improve the kinetics of latex agglutination reactions. The particles are stabilized by the positively-charged amine groups under low to neutral pH conditions. Care should be taken not to use them under high pH.
Microspheres Are Available in Many Sizes and Surface Functionalities
• 25 sizes to choose from—0.02 microns to 16 microns
• 20 different surface—for ready conjugation to virtually any material
• Surfactant free—ultraclean
• Various quantities—from milliliter to 500-liter amounts
Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.
We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
Total Exosome Isolation Reagent (from urine) Invitrogen™
Total Exosome Isolation Reagent (from urine) enables fast and efficient enrichment of intact exosomes from urine samples with a protocol that is scalable depending on your sample size.
• Maximize recovery of intact exosomes for any type of downstream application
• Easily isolate exosomes using our simple and reliable protocol
• Avoid time-consuming ultra-centrifugation
• Maximize flexibility—can be scaled up or down depending on the sample size
By tying up water molecules, the Total Exosome Isolation Reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the urine, and the solution is incubated for 1 hour at room temperature. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 1 hour at 2–8°C. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can be purified using the Total Exosome RNA and Protein Isolation Kit (Cat# 4478545), or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking.
For downstream mass spectrometry analysis we recommend performing gel electrophoresis purification of the isolated exosome suspension prior to analysis.
• Maximize recovery of intact exosomes for any type of downstream application
• Easily isolate exosomes using our simple and reliable protocol
• Avoid time-consuming ultra-centrifugation
• Maximize flexibility—can be scaled up or down depending on the sample size
By tying up water molecules, the Total Exosome Isolation Reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the urine, and the solution is incubated for 1 hour at room temperature. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 1 hour at 2–8°C. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can be purified using the Total Exosome RNA and Protein Isolation Kit (Cat# 4478545), or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking.
For downstream mass spectrometry analysis we recommend performing gel electrophoresis purification of the isolated exosome suspension prior to analysis.
5000 Series 0.31μm to 0.93μm Polymer Particle Suspensions Thermo Scientific™
Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 5000 Series Polymer Particle Suspensions, of special interest for research in light scattering, microporous filter checking and aerosol particle generation.
Cyto-Plex™ Carboxylated Particles Thermo Scientific™
Save time when measuring analytes with Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, open platform particles for suspension array analysis.
COUNT-CAL™ Count Precision Size Standards Thermo Scientific™
Minimize contamination by eliminating the need for serial dilutions and extensive sample handling with Thermo Scientific™ COUNT-CAL™ Count Precision Size Standards, a cost-effective, convenient and easy to use product for validating liquid particle counters. Packaged in single-use bottles and intended to be sampled directly from the bottle, standards were developed for manufacturers of parenteral drugs and ophthalmic solutions seeking interim verification of USP 788 and 789 (Particulate Matter in Injections and Particulate Matter in Ophthalmic Solutions).
7000 Series Copolymer Microsphere Suspensions Thermo Scientific™
Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 7000 Series Copolymer Microsphere Suspensions, useful as model systems for fluid mechanics experiments and as challenge particles for large pore filtration systems.
Aliphatic Amine Latex Beads, 2% w/v, 0.7 µm Invitrogen™
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.
This aliphatic amine latex has a high density of amine groups attached to the terminus of an aliphatic six-carbon spacer arm. This product can be used to covalently couple proteins. Location of the amine at the end of the spacer arm minimizes difficulties with steric hindrance and conformation since the group has a greater freedom to rotate. In addition, spacer arms improve the kinetics of latex agglutination reactions. The particles are stabilized by the positively-charged amine groups under low to neutral pH conditions. Care should be taken not to use them under high pH.
Microspheres Are Available in Many Sizes and Surface Functionalities
• 25 sizes to choose from—0.02 microns to 16 microns
• 20 different surface—for ready conjugation to virtually any material
• Surfactant free—ultraclean
• Various quantities—from milliliter to 500-liter amounts
Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.
We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
This aliphatic amine latex has a high density of amine groups attached to the terminus of an aliphatic six-carbon spacer arm. This product can be used to covalently couple proteins. Location of the amine at the end of the spacer arm minimizes difficulties with steric hindrance and conformation since the group has a greater freedom to rotate. In addition, spacer arms improve the kinetics of latex agglutination reactions. The particles are stabilized by the positively-charged amine groups under low to neutral pH conditions. Care should be taken not to use them under high pH.
Microspheres Are Available in Many Sizes and Surface Functionalities
• 25 sizes to choose from—0.02 microns to 16 microns
• 20 different surface—for ready conjugation to virtually any material
• Surfactant free—ultraclean
• Various quantities—from milliliter to 500-liter amounts
Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.
We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
Exosome-Human CD81 Isolation Reagent (from cell culture) Invitrogen™
Exosome-Human CD81 Isolation Reagent (from cell culture) enables isolation of CD81-positive extracellular vesicles (ECVs) such as exosomes from enriched cell culture media. Following isolation, the specific sub-population of exosomes/ECVs can be further studied using methods like Western blotting, qPCR, or next generation sequencing.
• Minimal hands-on time for specific exosome isolation
• Obtain a highly pure exosome subset
• Protocol is easily scalable
• Compatible with most downstream analysis
Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.
Dynabeads® magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads® magnetic separation technology also allows you to 'see' your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.
Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.
• Minimal hands-on time for specific exosome isolation
• Obtain a highly pure exosome subset
• Protocol is easily scalable
• Compatible with most downstream analysis
Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.
Dynabeads® magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads® magnetic separation technology also allows you to 'see' your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.
Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.
