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Target Organism Class

Dynabeads™ M-280 Streptavidin, for OEM and industrial use only Thermo Scientific™

Uniform and superparamagnetic Dynabeads®, 2.8 µm in diameter, with a monolayer of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The beads allow for efficient capture and downstream handling of your biotinylated ligand or target molecule. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results.

Streptavidin-coupled Dynabeads® is the gold standard for isolation and handling of biotinylated antibodies, nucleic acids or other biotinylated ligands and targets. The liquid-phase behavior in combination with the superparamagnetic properties of the beads provide rapid reaction kinetics both in the coating process, separation and during washing of the analyte.

The product holds reputable Dynal high standards with respect to reproducibility and automation ability, and drives reliability for your assays.

Benefits and Features:

• Direct and fast capture of any biotinylated ligand or target
• Flexible solid-phase protocols with superior liquid-phase reaction kinetics
• Easy and reproducible handling in manufacturing
• Low charged and neutral beads, optimal for binding of proteins, peptides and antibodies
• Fast and efficient washing procedures
• High batch-to-batch reproducibility, securing consistent results in your application
• Well suited for automated protocols

Applications:
Efficient capture of biotinylated molecules. For direct⁄indirect isolation and downstream handling of nucleic acids, proteins⁄peptides and other target molecules. Due to their uniformity, excellent batch reproducibility and ease of adaptation to automated processes, Dynabeads® have become the solid phase of choice for immunoassay development.

Binding capacity:
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-280 Streptavidin typically binds 650-900 pmoles free biotin, 200 pmol biotinylated peptides, up to10 µg biotinylated antibody, approx. 10 µg ds-DNA or 200 pmol ss-Oligonucleotides.

Additional Info:
This specific product format is for large volume customers, available on an OEM basis. The product is also available in smaller volumes for end-users (Cat. no 112-05D, 112-06D and 602-10).

9000 Series Glass Particle Standards Thermo Scientific™

Replace polystyrene spheres in many applications with Thermo Scientific™ 9000 Series Glass Particle Standards, dry glass NIST™-traceable size standards. Glass microspheres can be used in any application that requires a NIST™-traceable size standard with a narrow size distribution where sample conditions may not be suitable for polystyrene spheres. The glass microspheres provide a better tolerance to chemicals and solvents in addition to higher mechanical and thermal stability.

Exosome Immunoprecipitation Reagent (Protein G) Invitrogen™

Exosome Immunoprecipitation Reagent (Protein G) enables fast and gentle magnetic isolation of exosomal proteins, causing minimal physical stress to the target protein and allowing comparison of multiple samples on the same gel.

• Maintain intact exosome protein complexes
• Reduce background significantly—low non-specific binding
• Fast protocol time—only 30 minutes
• Maximal comparison—ultra-sensitivity allows many samples on the same gel

Dynabeads® coupled to Protein G are widely used for immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and protein isolation. The magnetic properties of Dynabeads® makes them a superior alternative to sepharose or agarose slurry for immunoprecipitation, since magnetic separation technology is faster and gentler than other methods, causing minimal physical stress to the target proteins. This permits the isolation and concentration of labile composites that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and intact, large protein complexes will be preserved.

Exosome Immunoprecipitation (Protein G) makes use of Dynabeads® Protein G for antibody binding and subsequent immunoprecipitation of exosomal proteins. Antibody is added to the Dynabeads® suspension where binding occurs via the Fc-region of the antibody. The mixture is then placed near a magnet, causing the beads migrate to the side of the tube, allowing easy removal of the supernatant. The bead-bound antibody can now be used for immunoprecipitation of exosomal proteins. Immunoprecipitation allows a 10 to 50 times concentration of exosomal proteins prior to protein analysis, such as Western blotting.

The amount of Ig captured by Exosome Immunoprecipitation (Protein G) is dependent on the concentration of Ig in the starting sample. The binding capacity is approximately 240 µg human Ig per mL beads.

For isolation of Ig via protein A, we recommend the Exosome Immunoprecipitation (Protein A).

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

5000 Series 0.03μm to 0.30μm Polymer Particle Suspensions Thermo Scientific™

Optimize research in light scattering, microporous filter checking, and aerosol particle generation with Thermo Scientific™ 5000 Series 0.03μm to 0.30μm Polymer Particle Suspensions. These particles are designed to meet the need for particulate materials with a variety of particle sizes and properties.

Amidine Latex Beads, 4% w/v, 0.2 µm Invitrogen™

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

This positively-charged hydrophobic amidine latex is particularly suited for the preparation of latex intermediates. The only surface functional group present on the particle is amidine, which is not sensitive to the aggregating effects of polyvalent cations. These particles are sensitive to negatively charged contaminants or multivalent anions. The amidine latexes should be used in low to neutral pH environments. The amidine group is a base and with a pK ~10-11.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

MagniSort™ Mouse CD45 Depletion Kit Invitrogen™

The MagniSort™ Mouse CD45 Depletion Kit is designed for the magnetic depletion of mouse CD45+ cells from mouse spleens, bone marrow or digested lung tissue. It utilizes a biotinylated Anti-Mouse CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by decanting.

After depletion, the purity of enriched CD45- cells can be verified by staining with Anti-Mouse CD45, clone 30-F11.

Reported Application
Magnetic Cell Separation

Cyto-Plex™ Carboxylated Particles Thermo Scientific™

Save time when measuring analytes with Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, open platform particles for suspension array analysis.

7000 Series Copolymer Microsphere Suspensions Thermo Scientific™

Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 7000 Series Copolymer Microsphere Suspensions, useful as model systems for fluid mechanics experiments and as challenge particles for large pore filtration systems.

SURF-CAL™ Particle Size Standards Thermo Scientific™

Simplify the preparation of calibration wafers with Thermo Scientific™ SURF-CAL™ Particle Size Standards, for calibration and maintenance of Scanning Surface Inspection Systems (SSIS). Available particle sizes correspond to the calibration point sizes required by instrument manufacturers.

LIVE/DEAD™ Fixable Red Dead Cell Stain Kit, for 488 nm excitation Invitrogen™

The LIVE/DEAD™ Fixable Red Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a blue laser flow cytometer.

• Stable—dyes are freeze dried in separate vials to maintain stability

• Robust—staining pattern is the same before and after fixation

• Bright signal—allows for easy distinction between live/dead cells in single channel

View a selection guide for all fixable viability dyes for flow cytometry.

Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Red Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Optimal brightness
The LIVE/DEAD™ Fixable Red stain was selected based on its fluorescent properties to provide a bright signal when excited with a yellow, green, or blue 488 nm laser. The red-fluorescent reactive dye has an excitation maximum of ~595 nm, making it ideal for use with the yellow laser, and an emission of ~615 nm, allowing collection in the 610 or 630 BP filter. LIVE/DEAD™ Fixable Red Dead Cell stain is also excited well with the blue 488 nm laser. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.

How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available
LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.

Amidine Latex Beads, 4% w/v, 0.5 µm Invitrogen™

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

This positively-charged hydrophobic amidine latex is particularly suited for the preparation of latex intermediates. The only surface functional group present on the particle is amidine, which is not sensitive to the aggregating effects of polyvalent cations. These particles are sensitive to negatively charged contaminants or multivalent anions. The amidine latexes should be used in low to neutral pH environments. The amidine group is a base and with a pK ~10-11.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Dynabeads™ Sheep-Anti Mouse IgG Invitrogen™

Dynabeads® Sheep anti-Mouse IgG binds monoclonal mouse IgG of your choice for specific cell isolation or depletion. All cells from all species except from mouse can be isolated with this product, depending only on the specific antibody of choice.

• Depletion: cells can be depleted from a sample
• Positive isolation: cells can be positively isolated from a sample for molecular applications
• Negative isolation: cells can be negatively isolated from a PBMC sample by adding an antibody cocktail of mouse IgGs towards the unwanted cells. Compatible with flow cytometry.

Note: For a more universal binding of your mouse IgG of any subclass, please use Dynabeads® Pan Mouse Ig (Cat.no. 110-41D or 110-42D).

Starting samples:
Whole blood, PBMC, buffy coat., tissue digests.

Exosome-Human CD9 Isolation Reagent (from cell culture) Invitrogen™

Exosome-Human CD9 Isolation Reagent (from cell culture) enables isolation of CD9-positive extracellular vesicles (ECVs) such as exosomes from enriched cell culture media. Following isolation, the specific sub-population of exosomes/ECVs can be further studied using methods like Western blotting, qPCR, or next generation sequencing.

• Minimal hands-on time for specific exosome isolation

• Obtain a highly pure exosome subset

• Protocol is easily scalable

• Compatible with most downstream analysis

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.

Dynabeads® magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads® magnetic separation technology also allows you to 'see' your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.

4000 Series Monosized Particles Thermo Scientific™

Minimize response of analytic systems to shape effects with Thermo Scientific™ 4000 Series Monosized Particles, excellent for use with any application that requires a NIST™ traceable size standard with a very narrow standard deviation.

Dyed Red Aqueous Fluorescent Particles Thermo Scientific™

Improve sensitivity and detectability for analytical methods with Thermo Scientific™ Fluoro-Max Red Aqueous Fluorescent Particles, microsphere suspensions that are internally dyed and feature bright, high-contrast colors.
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