Shop All Microspheres

Target Organism Class

3K/4K Series Particle Counter Standards Thermo Scientific™

Ensure accurate and convenient calibration of laser particle counters with Thermo Scientific™ 3K/4K Series Particle Counter Standards. Compared with our 3000 and 4000 Series products, this product line is precisely diluted for immediate use in laser particle counters with minimal time-consuming concentration adjustments.

Dynabeads™ Protein A and Magnet Starter Pack Invitrogen™

The Dynabeads Protein A and Magnet Starter Pack combines Dynabeads Protein A magnetic beads for immunoprecipitation with the magnet preferred for use with 1.5 mL microcentrifuge tubes for purchasing convenience. Each mL of beads is sufficient for 20 reactions, and the magnet holds 16 x 1.5 mL microcentrifuge tubes. Dynabeads Protein A beads and the DynaMag-2 magnet are also available separately.

Opti-Bind™ Polystyrene Sulfate Particles Thermo Scientific™

Achieve maximum reactivity in many diagnostic applications with Thermo Scientific™ Opti-Bind™ Polystyrene Sulfate Particles for use in turbidimetric/nephelometric assays, slide agglutination assays, chemiluminescent assays and in clinical diagnostics and molecular biology applications. Particles can be used direct from the bottle, without any pre-cleaning.

Dyed Red Aqueous Fluorescent Particles Thermo Scientific™

Improve sensitivity and detectability for analytical methods with Thermo Scientific™ Fluoro-Max Red Aqueous Fluorescent Particles, microsphere suspensions that are internally dyed and feature bright, high-contrast colors.

CML Latex Beads, 4% w/v, 0.5 µm Invitrogen™

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

These carboxylate modified (CML) latex particles are produced by copolymerizing carboxylic acid containing polymers. The result is a latex polymer particle with a highly charged, relatively hydrophilic and somewhat 'fluffy' surface layer. The CML particles are electrosterically stabilized, and are therefore safe in concentrations of electrolyte up to 1M univalent salt. CML modified latex particles are negatively charged with a surface which has a polyelectrolyte character. It is only when the pH is ~10 that all the carboxyl groups are ionized. This is a hydrophilic latex.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

9000 Series Glass Particle Standards Thermo Scientific™

Replace polystyrene spheres in many applications with Thermo Scientific™ 9000 Series Glass Particle Standards, dry glass NIST™-traceable size standards. Glass microspheres can be used in any application that requires a NIST™-traceable size standard with a narrow size distribution where sample conditions may not be suitable for polystyrene spheres. The glass microspheres provide a better tolerance to chemicals and solvents in addition to higher mechanical and thermal stability.

SURF-CAL™ Particle Size Standards Thermo Scientific™

Simplify the preparation of calibration wafers with Thermo Scientific™ SURF-CAL™ Particle Size Standards, for calibration and maintenance of Scanning Surface Inspection Systems (SSIS). Available particle sizes correspond to the calibration point sizes required by instrument manufacturers.

OneComp eBeads™ Compensation Beads Invitrogen™

OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. The beads are spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls.

Each drop of beads contains two populations: a positive population that will capture any mouse, rat or hamster antibody and a negative population that will not react with antibody. When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations result. This bimodal distribution can be used for single-color compensation controls in multicolor flow cytometry experiments.

OneComp eBeads cross-react to some antibodies of rabbit origin, but have not been extensively tested for this reactivity. OneComp eBeads are designed for use in compensation with all fluorochromes excited by blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-635 nm) lasers. This product is compatible with eFluor™ 450 but is not optimized for compensation of other fluorochromes excited by a violet (405 nm) laser.

Reactivity/Species
Hamster, Mouse, Rat

Reported Application
Flow Cytometric Analysis

Opti-Link™ Carboxylate-Modified Particles Thermo Scientific™

Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles. Particles contain carboxylic acid groups for covalent coupling and can be used directly from the bottle in a variety of applications.

CML Latex Beads, 4% w/v, 1.0 µm Invitrogen™

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

These carboxylate modified (CML) latex particles are produced by copolymerizing carboxylic acid containing polymers. The result is a latex polymer particle with a highly charged, relatively hydrophilic and somewhat 'fluffy' surface layer. The CML particles are electrosterically stabilized, and are therefore safe in concentrations of electrolyte up to 1M univalent salt. CML modified latex particles are negatively charged with a surface which has a polyelectrolyte character. It is only when the pH is ~10 that all the carboxyl groups are ionized. This is a hydrophilic latex.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

5000 Series 0.03μm to 0.30μm Polymer Particle Suspensions Thermo Scientific™

Optimize research in light scattering, microporous filter checking, and aerosol particle generation with Thermo Scientific™ 5000 Series 0.03μm to 0.30μm Polymer Particle Suspensions. These particles are designed to meet the need for particulate materials with a variety of particle sizes and properties.

Attune™ Performance Tracking Beads

The Attune Performance Tracking Beads are designed for use with Attune Cytometric Software and Attune NxT Software to automatically characterize, track, and report the performance of the Attune Acoustic Focusing Cytometer and Attune NxT Acoustic Focusing Cytometer daily and over time. Regular calibration of your instrument with these beads will ensure the accuracy and sensitivity of the instrument and allow easy identification of potential shifts or trends in instrument performance. The tracking beads contain distinct sub-populations of beads labeled at different intensities, and are used to establish optimal dynamic range settings for the instrument. The beads are pre-calibrated at the factory and the lot-specific bead performance data captured as a downloadable file for use by the Attune Cytometric/Attune NxT software. Each vial contains enough beads for 50 measurements (Attune) or 25 measurements (Attune NxT). The beads are provided at a concentration of 5 × 106 beads/mL.

Bead lot files:

Attune NxT
Attune (non NxT)

Learn more about the Attune Acoustic Focusing cytometers

Dynabeads™ Oligo(dT)25 Invitrogen™

Dynabeads® Oligo(dT)25 mRNA isolation beads specifically target and capture mRNA molecules from virtually any crude sample and eliminate the need to purify total RNA when the desired information-bearing nucleic acid is mRNA. Since mRNA comprises only about 1–5% ot total cellular RNA, the isolation of total RNA is not the most efficient way to isolate mRNA. Other technologies designed to purify total RNA yield ~80% ribosomal RNA and force mRNA to compete with ribosomal RNA, transfer RNA, micro RNA, small nucleolar RNA, and small cytoplasmic RNA for membrane binding. Advantages of Dynabeads® Oligo(dT)25 beads:

• Fast and gentle procedure yields pure intact mRNA
• Extremely pure mRNA isolation, best choice upstream of cDNA synthesis
• Exquisitely sensitive mRNA isolation enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell)

How the beads work
The oligo(dT)25-coated Dynabeads® specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured. Isolated mRNA is pure, eliminating the need for ribosomal RNA subtraction or a post-extraction DNase treatment. This column-free system ensures the highest transcriptome recovery:

• Physical mRNA capture on mobile magnetic beads
• Rapid and gentle magnetic handling procedures
• No mRNA lost during high g-force spins
• No mRNA trapped in column membranes during elution

Applications
mRNA is suitable for all downstream molecular applications, including gene cloning, cDNA synthesis, cDNA library construction, RT-PCR, quantitative RT-PCR, RPA (Ribonuclease Protection Assay), subtractive hybridization, primer extension, SAGE, RACE, and others. The Dynabeads® Oligo(dT)25 mRNA isolation beads are the ideal mRNA purification method prior to cDNA library construction. Use of these beads ensures the highest recovery and enrichment of the transcriptome. These beads capture more of the transcriptome than is possible with methods that integrate a total RNA isolation step upstream of mRNA isolation.

Verastile elution options
Elution can be performed in any volume down to 5 µL. mRNA elution is optional because enzymatic reactions in downstream procedures are not inhibited by presence of Dynabeads®. Additionally, one can perform cDNA synthesis directly on the beads to create a reusable solid-phase cDNA library.

5000 Series 0.31μm to 0.93μm Polymer Particle Suspensions Thermo Scientific™

Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 5000 Series Polymer Particle Suspensions, of special interest for research in light scattering, microporous filter checking and aerosol particle generation.

123count eBeads™ Counting Beads Invitrogen™

123count eBeads are intended for use in absolute counting of cells or other particles by flow cytometry. These are 7 μm microparticles containing encapsulated dyes compatible with blue (488 nm) and violet (405 nm) excitation sources and emitting fluorescence between approximately 500 nm and 750 nm. They are supplied at a known concentration in 0.05% Tween-20 with 2 mM sodium azide. Collecting carefully suspended and measured quantities of cell:bead mixtures on a flow cytometer makes the ratiometric enumeration of cells possible.

Reported Application
Flow Cytometric Analysis

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