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Target Organism Class

SURF-CAL™ Particle Size Standards Thermo Scientific™

Simplify the preparation of calibration wafers with Thermo Scientific™ SURF-CAL™ Particle Size Standards, for calibration and maintenance of Scanning Surface Inspection Systems (SSIS). Available particle sizes correspond to the calibration point sizes required by instrument manufacturers.

7000 Series Copolymer Microsphere Suspensions Thermo Scientific™

Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 7000 Series Copolymer Microsphere Suspensions, useful as model systems for fluid mechanics experiments and as challenge particles for large pore filtration systems.

Fluoro-Max Dyed Blue Aqueous Fluorescent Particles Thermo Scientific™

Achieve improved sensitivity and detectability in analytical methods with Thermo Scientific™ Fluoro-Max Blue Aqueous Fluorescent Particles, internally dyed microsphere suspensions that feature bright, high-contrast colors.

4000 Series Monosized Particles Thermo Scientific™

Minimize response of analytic systems to shape effects with Thermo Scientific™ 4000 Series Monosized Particles, excellent for use with any application that requires a NIST™ traceable size standard with a very narrow standard deviation.

9000 Series Glass Particle Standards Thermo Scientific™

Replace polystyrene spheres in many applications with Thermo Scientific™ 9000 Series Glass Particle Standards, dry glass NIST™-traceable size standards. Glass microspheres can be used in any application that requires a NIST™-traceable size standard with a narrow size distribution where sample conditions may not be suitable for polystyrene spheres. The glass microspheres provide a better tolerance to chemicals and solvents in addition to higher mechanical and thermal stability.

Dynabeads™ Untouched™ Mouse CD4 Cells Kit Invitrogen™

Dynabeads® Untouched™ Mouse CD4 Cells contains both magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse CD4+ cells. The negatively isolated, untouched mouse CD4+ cells are pure, viable and ready to be used in any downstream application.
• Rapid isolation of untouched mouse CD4+ T-cells – no columns required
• Pure and viable mouse CD4+ T-cells ready for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD8+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-CD4+ cells. The cells are then washed to remove excess antibodies, and Mouse Depletion Dynabeads® are added. After a short incubation, the bead-bound cells are then separated from unbound cells in 1 – 2 minutes using a magnet. The purified target mouse CD4+ cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD4+ cells. The protocol can easily be scaled up according to your sample size.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Dynabeads™ SILANE Viral NA Kit Invitrogen™

For sensitive, highly predictable and consistent extraction and isolation of viral DNA and RNA from human serum/plasma samples, following a simple separation protocol. The 1 µm Dynabeads have an optimized silica-like surface chemistry, and a high specific surface area, providing efficient kinetics and a high sensitivity. Their uniform size and surface area ensure reproducible results.

A higher sensitivity and improved performance is achieved with Dynabeads® compared to magnetic particles from alternative suppliers. Other particles often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. Dynabeads® hold reputable Dynal high standards with respect to within- and between-lot reproducibility and automation ability.

Advantages:

• Isolates viral DNA and viral RNA
• High sensitivity, allowing for a low detection limit
• Effective isolation of nucleic acids even from challenging viruses (e.g. HBV)
• Can detect HBV DNA in a 200 µl serum sample containing only 40 infectious units (using 2 mg (50 µl) Dynabeads®)
• Quicker than spin-columns
• Cost-effective and performs to the same level as spin-columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)

Applications:
• Isolation of viral nucleic acids (DNA/RNA)
• From human serum/plasma samples
• Well suited for automated assays
• Separate kit available for the isolation of genomic DNA (Cat. no. 370-12D)

Additional Info:
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information, please contact us at: ivd@invitrogen.com

DynaMag™-96 Bottom Magnet Invitrogen™

96-well Magnet optimized for use with Dynabeads® This magnet pulls beads to the bottom of each well. Compatible with PCR-strips and 96-well plates (200ul) non-skirted and half-skirted. This magnet replaces the Dynal MPC®-96B.

When used in combination with the DynaMag™-96 Side (Product # 12331D) on an automation platform, the two magnets allow for significant volume changes through a workflow.
High wash volumes - DynaMag™-96 Side
Low Elution Volumes - DynaMag™-96 Bottom

Concentrates Beads at the bottom of each well.

Features 96 round magnets (one centered at the bottom of each well: to ensure identical sample handling at each position).

Allows for concentrating the sample by eluting in tiny volumes (~10 µl).

MagniSort™ Human CD8 Positive Selection Kit Invitrogen™

The MagniSort™ Human CD8 Positive Selection Kit is designed for the magnetic separation of CD8+ cells by positive selection. It has been optimized for the isolation of CD8+ cells from human peripheral blood monocuclear cells, utilizing a biotinylated Anti-Human CD8 antibody and streptavidin-coated magnetic beads. CD8+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD8+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD8, clones RPA-T8 or SK1.

Reported Application
Magnetic Cell Separation

Cyto-Plex™ Carboxylated Particles Thermo Scientific™

Save time when measuring analytes with Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, open platform particles for suspension array analysis.

Color-Rich Dyed Carboxylate-Modified Particles Thermo Scientific™

Obtain superior test sensitivity in qualitative and quantitative lateral flow tests with Thermo Scientific™ Color-Rich Dyed Carboxylate-Modified Particles. Higher intensity dyed particles provide maximum color and brilliance, as well as immunologically reactive surfaces. These particles are composed of polystyrene with or without a copolymer grated surface for fast coupling and processing reactions. Available in blue, red or black internally dyed versions, these hydrophilic particles can bind ligands without any dye interference, feature high protein binding capacity; and have an optimized acid content.

5000 Series 0.31μm to 0.93μm Polymer Particle Suspensions Thermo Scientific™

Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 5000 Series Polymer Particle Suspensions, of special interest for research in light scattering, microporous filter checking and aerosol particle generation.

Dynabeads™ SILANE Genomic DNA Kit Invitrogen™

For highly predictable and consistent extraction and isolation of genomic DNA from human whole blood, following a simple separation protocol. Isolates linear DNA yields relative to the number of white blood cells (WBC) present in the sample. The 1 µm Dynabeads® have an optimized silica-like surface chemistry and a high specific surface area, providing efficient kinetics and a high capacity.

Their uniform size and surface area ensure highly reproducible results and an improved performance compared to magnetic particles from alternative suppliers. Other particles often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. Dynabeads® hold reputable Dynal high standards with respect to within- and between-lot reproducibility and automation ability.

Advantages:

• Highly predictable binding per mg of beads
• Linear range of DNA yield relative to WBC-count
• High integrity and purity of isolated DNA
• Isolates 10 µg DNA from a 350 µl blood sample using 2 mg (50 µl) Dynabeads®
• Quicker than spin-columns
• More cost-effective, yet performs to the same level as spin-columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)

Applications
• Isolation of genomic DNA
• From cells and fresh/frozen blood samples
• From blood with a wide range of anticoagulants
• Well suited for automated assays
• Separate kit available for the isolation of viral DNA/RNA (Cat. no. 370-11D)

Additional Info:
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information, please contact us at: ivd@invitrogen.com

Dynabeads™ Human T-Expander CD3/CD28 Gibco™

Dynabeads® Human T-Expander CD3/CD28 is intended for separation, activation and expansion of human T cells. Dynabeads® Human T-Expander CD3/CD28 is the research grade version of Dynabeads® CD3/CD28 CTS™. Containing the same amount and ratio of antibodies from the same clones as Dynabeads® CD3/CD28 CTS™, Dynabeads® Human T-Expander CD3/CD28 are intended for small scale pre-clinical research. Dynabeads® Human T-Expander CD3/CD28 offers a simple method for activation and expansion of T-cells that does not require antigen presenting cells or antigen. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads provide both the primary and co-stimulatory signals that are required for activation and expansion of T cells.

Applications:
Dynabeads® Human T-Expander CD3/CD28 is the research grade version of Dynabeads® CD3/CD28 CTS™. The Dynabeads® CD3/CD28 CTS™ technology has been successfully used in ex vivo separation, activation and expansion of T-cells in a number of studies, including studies on HIV infection and cancer. Other potential applications for this novel technology are treatment of autoimmune and infectious diseases and prevention of complications associated with chemotherapy and allogeneic transplantation.

Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit Invitrogen™

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells

Treg cells can be expanded with Dynabeads® CD3/CD28 Treg Expander and remain
Foxp3+.

Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology

The isolation procedure includes the following steps:

• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD®.

Starting sample: PBMC
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