Shop All Cellular Imaging Instrumentation Accessories

EVOS™ Vessel Holder for Oil Objectives, one 25 x 75 mm microscope slide Invitrogen™

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one 25 mm x 75 mm microscope slide is designed to provide the greater clearance needed when using oil objectives. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Vessel Holder for Automated Stage, one Terasaki plate Invitrogen™

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one Terasaki plate and is specific for the automated stage of the EVOS FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

Tali™ Image-Based Cytometer Power Cord Invitrogen™

This power cord is a replacement part for the Tali™ Image-Based Cytometer instrument. The Tali™ Image-Based Cytometer Power cord and the four universal adaptors included have been specifically designed for use with the Tali™ Image-Based Cytometer instrument. This product has been CE certified.

GeneMapper™ Software 6, full installation Applied Biosystems™

GeneMapper Software is a flexible genotyping software package that provides DNA sizing and quality allele calls for all Thermo Fisher Scientific electrophoresis-based genotyping systems. This software specializes in multi-application functionality, including amplified fragment length polymorphism (AFLP), loss of heterozygosity (LOH), microsatellite and SNP genotyping analysis. GeneMapper Software can help users increase data processing efficiency with remote auto-analysis and command line operation, and allows for multiuser, client-server deployment. The software uses Process Quality Values (PQVs) for automated identification that reduces data review time for high throughput genotyping. In addition, the security and audit features help users meet 21 CFR 11 requirements.

Register to download a trial version of this software.

Features include:

• Installation on the data collection computer or a non-instrument computer
• Support of networked multiple clients when installed on a non-instrument computer
• An Oracle 11g database with project-related data storage that ensures faster installation times and latest database support, as well as flexible archive capabilities
• Customizable project auto-saving frequency
• Defining of linearity range in the analysis methods for more reproducible, higher quality sizing of larger fragments (>800bps)
• Analysis of 6-dye data generated from the Applied Biosystems 3500 and 3500xL instrument systems for higher multiplexing capabilities

GeneMapper Software has not undergone specific developmental validation for human identification applications. Human identification laboratories analyzing single-source or parentage samples that choose to use GeneMapper Software for data analysis should perform their own developmental validation studies.

Several users may access the database concurrently; however, because genotype analysis is data-intensive, more than five clients simultaneously accessing the database may slow the performance of GeneMapper Software.

Recommended Computer Configuration: Dell OptiPlex XE2, 7-4770S Intel Processor (Quad Core HT, 3.10GHz Turbo, 8 MB), 16 GB (2x8 GB) 1600 MHz DDR3. Support Operating Systems: Windows 10 Pro/IoT and Windows 7 Professional Service Pack 1

EVOS™ Vessel Holder for Automated Stage, one Falcon T-75 flask or standard micro-well plate Invitrogen™

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one Falcon T-75 flask or standard micro-well plate and is specific for the automated stage of the EVOS FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

InSpeck™ Green (505/515) Microscope Image Intensity Calibration Kit, 2.5 µm Invitrogen™

The InSpeck™ Green Microscopy Image Intensity Calibration Kit provides microsphere standards that generate a series of well-defined fluorescent intensity levels for constructing calibration curves and evaluating sample brightness. Each kit includes six separate suspensions of 2.5 µm fluorescent microspheres with relative fluorescence intensities of 100%, 30%, 10%, 3%, 1% and 0.3% covering the range of intensities commonly encountered in microscopy applications. Unstained control beads and mounting medium are also supplied. The aqueous suspensions of microspheres may be applied directly to the sample for calibrating fluorescence intensities or mounted separately in an adjacent well or on another slide.

EVOS™ Onstage Vessel Holder, one Lab-Tek coverglass chamber slide Invitrogen™

EVOS Onstage vessel holders maximize your choice of cell culture vessels when conducting time-lapse imaging experiments using the EVOS FL Auto Imaging System equipped with an EVOS Onstage Incubator. EVOS Onstage vessel holders allow a perfect fit of your micro-well plates, cell culture flasks/dishes, and slides to the master plate of the EVOS Onstage Incubator for increased precision in sample alignment. This particular vessel holder accommodates one Nunc Lab-Tek coverglass chamber slide. With a snug and secure fit, yet readily switched, these vessel holders make time-lapse imaging convenient and easy.

Learn more about the EVOS FL Auto Imaging System >

Explore the entire EVOS line of imaging systems and accessories >

Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-mouse IgG Invitrogen™

SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.

SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 488 tyramide (496/524 ex/em), detected using a standard Green/FITC/GFP filter cube. This kit also features poly-HRP-conjugated goat anti-mouse IgG secondary antibody.

Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.

Benefits of SuperBoost kits

Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.

Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.

Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.

Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.

Alexa Fluor™ 594 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG Invitrogen™

SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.

SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 594 tyramide (591/617 ex/em), detected using a standard Red/Texas Red™ filter cube. This kit also features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody.

Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.

Benefits of SuperBoost kits

Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.

Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.

Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.

Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.

EVOS™ Vessel Holder for Automated Stage, one Nunc™ multi-well dish Invitrogen™

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one Nunc multi-well dish and is specific for the automated stage of the EVOS FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

Tali™ Cellular Analysis Slides Invitrogen™

The Tali® Cellular Analysis Slides are specifically designed for use with the Tali® Image-Based Cytometer. Each slide contains two separate, enclosed analysis chambers designed to accommodate 25 µl of sample per chamber. Each box contains 50 slides.

Ready to Use for Your Convenience
Each Tali® Cellular Analysis slide is pre-sterilized and individually packaged. Since the Tali® Image-Based Cytometer is a fluorescent cellular analysis instrument the slides are composed of low fluorescent plastic. The slides are disposable; after use the slides can be disposed of with you regular biohazard waste.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Learn more about the the Tali® Image-based Cytometer

Celleste™ 5.0 Image Analysis Software Invitrogen™

Celleste 5.0 Image Analysis Software has features designed for a range of biological applications, such as image adjustment and processing with manual and automatic measurements over multiple channels, and segmentation and classification tools that help you transform images into quantitative data in a streamlined and customizable workflow. Optional modules for deconvolution, 3D visualization, and 3D analysis allow you to customize the capabilities according to your needs. Coupled with the powerful image acquisition capabilities of our EVOS microscopes, Celleste software allows you to seamlessly capture, process, measure, analyze, and share images and data.

Celleste 5.0 Image Analysis Software offers you these important advantages:
• Streamlined workflow tools that make it easy to visualize and edit images
• Comprehensive image processing and enhancement functions with optional modules to meet your specific needs
• Powerful image analysis capabilities for segmentation, classification, and quantification of single images or a batch of images
• A new interface for streamlined multi-well plate navigation and well selection
• A wide range of functions to create reports and share images and data

Process and analyze
After image acquisition, immediately apply a suite of image processing and enhancement functions with a few clicks of a mouse. Use auto-alignment for overlays and remove background to reveal critical details with a wide range of filters. Once satisfied with the results from one image, apply the same steps to a set of time-lapse images, scans, or results from different treatments. When imaging cells in multi-well plates, use the new interface to select only the wells you wish to analyze and automatically process them as a set.

Measure and quantify
Easily measure and analyze your images with a variety of measurement tools, such as those for distance, region, angles, and area. The ability to identify an object in time-lapse experiments can be used to track cell movement or migration. This feature is particularly powerful in combination with the EVOS Onstage Incubator, which allows continuous monitoring of cells under controlled environmental conditions.

Count and classify
Using a number of manual and automatic measurement tools, easily count and characterize cells and subcellular objects based on area, length, shape, and light intensity. Flexible segmentation tools allow you to label images based on color or intensity level. A new ROI functionality allows you to count objects and maintain measurements by region (e.g., a parent-child application) and also to count by ROI. After object counting, sort and display counted images based on size and other parameters.

Report and share
Upon completing the image analysis, a suite of annotation and reporting tools allow you to create presentation-ready image and data reports with a few clicks and to share them with others in PDF, PowerPoint, and Excel format.

Celleste 5.0 software is a powerful image analysis solution that helps make your imaging research job easier and increase your productivity by seamlessly providing images and accurate data. With the proven usability of EVOS microscopes, you now have the tools to make it easy to capture, process, measure, analyze, and share your important images and data more effectively than ever.

Coverglass Removal Tool Invitrogen™

The Coverglass Removal Tool is intended for use with a CultureWell™ Chambered Coverglass for Cell Culture (Cat. Nos. C37000, C37005). This tool enables you to easily separate the chambered upper structure from the coverglass without the need for excessive force, minimizing the risk of coverglass breakage.

GeneArt™ Service TSE Free Production Thermo Scientific™

The GeneArt® Gene Synthesis service offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence.

InSpeck™ Blue (350/440) Microscope Image Intensity Calibration Kit, 2.5 µm Invitrogen™

The InSpeck™ Blue Microscopy Image Intensity Calibration Kit provides microsphere standards that generate a series of well-defined fluorescent intensity levels for constructing calibration curves and evaluating sample brightness. Each kit includes six separate suspensions of 2.5 µm fluorescent microspheres with relative fluorescence intensities of 100%, 30%, 10%, 3%, 1% and 0.3% covering the range of intensities commonly encountered in microscopy applications. Unstained control beads and mounting medium are also supplied. The aqueous suspensions of microspheres may be applied directly to the sample for calibrating fluorescence intensities or mounted separately in an adjacent well or on another slide.
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