Enzymes and Inhibitors

A wide variety of modifying and restriction enzymes, buffers, and substrates in various quantities and concentrations. Products are designed for use with clinical, research-based, and general-purpose molecular biology applications.
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T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1).
DNase I Invitrogen™
DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5' phosphate. Ribonuclease has been reduced to non-detectable levels. Applications DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating...
HindIII (10 U/μL) Thermo Scientific™
Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions.
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active: • Over a wide pH range–optimal activity between 6.5 and 9.5 • Under denaturing conditions–e.g.
DNA-free™ DNase treatment and removal reagents are designed for the removal of contaminating DNA from RNA samples and for the removal of DNase after treatment. Features of this reagent set include: • Safely eliminate DNA contamination from RNA samples • No organic extraction or heat inactivation...
FastDigest AdeI Thermo Scientific™
Lambda DNA digested with DraIII (AdeI), 0.7% agarose, 10 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
RNase H (5 U/μL) Thermo Scientific™
Ribonuclease H (Rnase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
BamHI (10 U/μL) Thermo Scientific™
Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions.
NTP Set, 100 mM Solution Thermo Scientific™
The Thermo Scientific NTP set is a convenient set of 100 mM solutions of each ATP, CTP, GTP and UTP. These highly stable nucleotides are more than 99% pure and can be used in a variety of molecular biology applications.
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active: • Over a wide pH range—optimal activity between 6.5 and 9.5 • Under denaturing conditions—e.g.
Exonuclease VII Thermo Scientific™
Exonuclease VII is a strict single-strand directed enzyme with 5'→3' and 3'→5' exonuclease activities, making it the only bi-directional exonuclease with single-stranded specificity. Exonuclease VII has no apparent requirement for divalent cations, as it is fully active in the presence of EDTA.
FastDigest Eam1104I Thermo Scientific™
Lambda DNA digested with EarI (Eam1104I), 1% agarose, 2 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
E. coli DNA Ligase Invitrogen™
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme. Applications: Second-strand cDNA synthesis (1).
Anza™ 7 BshTI Invitrogen™
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification.
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Thermo Fisher Scientific is a global leader in the manufacture and supply of nucleic acid modifying enzymes and polymerases. We offer a combination of proprietary as well as generic off-the-shelf enzymes, and can also custom-manufacture on your behalf.
Protein enrichment encompasses numerous techniques to isolate cellular proteins based on their sequence, antibody epitope, post-translational modification (PTM) or active binding site. Protein enrichment is essential for isolating low-abundant proteins and/or reducing sample complexity for proteomic...
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What are active site probes?
What are active site probes and why are they useful as protein labels?