Modifying Enzymes

Modifying Enzymes

Enzymes that structurally alter macromolecules while retaining their own molecular structures. Various modifying enzymes, enzyme mixes, kits, buffers, etc. are available in multiple quantities and concentrations tailored to molecular biology procedures.
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1-15 of 125 Products

DNase I Invitrogen™

DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5' phosphate. Ribonuclease has been reduced to non-detectable levels. Applications DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating...

TURBO DNA-free™ Kit Invitrogen™

The TURBO DNA-free™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Note: if you would like to purchase the enzyme alone, without the inactivation and cation removal reagents, please see TURBO™ DNAase.

DNase I, RNase-free (1 U/μL) Thermo Scientific™

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

T4 DNA Ligase (5 U/μL) Thermo Scientific™

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Gateway™ LR Clonase™ II Enzyme mix Invitrogen™

Gateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression clone.

CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL Invitrogen™

Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA. Concentration: 1 unit/μL Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1).

DNA-free™ DNA Removal Kit Invitrogen™

DNA-free™ DNase treatment and removal reagents are designed for the removal of contaminating DNA from RNA samples and for the removal of DNase after treatment. Features of this reagent set include: • Safely eliminate DNA contamination from RNA samples • No organic extraction or heat inactivation...

Proteinase K, recombinant Invitrogen™

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active: • Over a wide pH range–optimal activity between 6.5 and 9.5 • Under denaturing conditions–e.g.

Gateway™ BP Clonase™ Enzyme Mix Invitrogen™

Gateway BP Clonase® enzyme contains both Int (Integrase) and IHF (Integration Host Factor) proteins that catalyze the in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and a Donor vector (containing attP sites) to generate Entry clones.

LR Clonase™ II Plus enzyme Invitrogen™

Gateway® LR Clonase® enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR...

Proteinase K Lyophilized Powder, Thermo Scientific Chemicals Thermo Scientific Chemicals

Used in the isolation of native, high molecular weight DNA and RNA. Used as a general protease to inactivate DNases, RNases and to degrade proteins. Specifically modifies cell surface proteins and glycoproteins for analysis of membrane structures for protein localization.

ezDNase™ Enzyme Invitrogen™

Invitrogen™ ezDNase™ Enzyme is a recombinant double-strand-specific DNase for the fast removal of contaminating genomic DNA from RNA preparations. It cleaves phosphodiester bonds in double-stranded DNA to yield 2–8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini.

Topoisomerase I Invitrogen™

Topoisomerase I (DNA-relaxing enzyme) catalyzes the removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds. Topoisomerase I is active in the presence of EDTA. Applications: Relaxing positively and negatively supercoiled DNA (1).

Proteinase K Solution Invitrogen™

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g.

CorrectASE™ Enzyme Invitrogen™

CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments. By introducing CorrectASE™ enzyme into your do-it-yourself gene syntheis workflow, you can: • Reduce the number of mutations in your...