Modifying Enzymes

Modifying Enzymes

Enzymes that structurally alter macromolecules while retaining their own molecular structures. Various modifying enzymes, enzyme mixes, kits, buffers, etc. are available in multiple quantities and concentrations tailored to molecular biology procedures.
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1-15 of 130 Products

Gateway™ LR Clonase™ II Enzyme mix Invitrogen™

Gateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression clone.

T4 DNA Ligase (5 U/μL) Thermo Scientific™

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

LR Clonase™ II Plus enzyme Invitrogen™

Gateway® LR Clonase® enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR...

T4 DNA Ligase, HC (30 U/μL) Thermo Scientific™

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

DNase I, RNase-free (1 U/μL) Thermo Scientific™

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

Terminal Deoxynucleotidyl Transferase (20 U/μL) Thermo Scientific™

Thermo Scientific Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA.

LR Clonase II for BaculoDirect™ Kits Invitrogen™

The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway® Technology with baculovirus expression makes the BaculoDirect™ System the fastest and easiest method for production of...

Gateway™ LR Clonase™ Enzyme Mix Invitrogen™

Gateway® LR Clonase® enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR...

Anza™ DNA Blunt End Kit Invitrogen™

The Invitrogen™ Anza™ DNA Blunt End Kit is used to convert DNA with cohesive ends to DNA with blunt ends for use in blunt-end ligation reactions. The included Anza™ DNA Blunting Enzyme Mix contains T4 DNA polymerase and Klenow Fragment, while the Anza™ 10X Blunting Buffer contains dNTPs to...

T4 DNA Ligase, LC (1 U/μL) Thermo Scientific™

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Bacterial Alkaline Phosphatase Invitrogen™

Bacterial Alkaline Phosphatase (BAP) removes 3´ and 5´ phosphates from DNA and RNA. BAP is active at 65°C for at least 1 h and is inactivated by phenol extraction. Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation.

RNase Cocktail™ Enzyme Mix Invitrogen™

Ambion RNase Cocktail is a mixture of two highly purified ribonucleases, RNase A (500 U⁄ml) and RNase T1 (20,000 U⁄ml) and is free of DNase and nicking activities. Use RNase Cocktail for all situations where it is desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays.

TrueCut™ Cas9 Protein v2 Invitrogen™

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency. Features include: • Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in...

RapidOut DNA Removal Kit Thermo Scientific™

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heating or organic extraction.

TheraPure™ GMP DNase I, RNase-free, 50 U/μL Thermo Scientific™

Thermo Scientific TheraPure GMP* DNase I, RNase-free, is manufactured to ICH Q7 GMP principles and supported by a comprehensive documentation package. It is designed for DNA template removal during the development and manufacturing of mRNA therapeutics and vaccines.
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