Modifying Enzymes | Thermo Fisher Scientific

DNase I Invitrogen™

DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5' phosphate. Ribonuclease has been reduced to non-detectable levels. Applications DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating...

T4 DNA Ligase (5 U/µL) Thermo Scientific™

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Gateway™ BP Clonase™ II Enzyme mix Invitrogen™

Gateway™ BP Clonase™ II enzyme mix catalyzes the in vitro recombination of PCR products or subcloning DNA segments from clones (containing attB sites) and a donor vector (containing attP sites) to generate entry clones.

DNase I, RNase-free (supplied with MnCl2) (1 U/µL) Thermo Scientific™

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

DNase I, RNase-free (1 U/µL) Thermo Scientific™

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

RNase I (10 U/µL) Thermo Scientific™

Thermo Scientific Ribonuclease I (RNase I) is an endoribonuclease that preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates. The enzyme does not require any metal ions for activity.

Terminal Deoxynucleotidyl Transferase (20 U/µL) Thermo Scientific™

Thermo Scientific Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA.

ezDNase™ Enzyme Invitrogen™

Invitrogen™ ezDNase™ Enzyme is a recombinant double-strand-specific DNase for the fast removal of contaminating genomic DNA from RNA preparations. It cleaves phosphodiester bonds in double-stranded DNA to yield 2–8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini.

Ambion™ RNase I, cloned, 100 U/µL Invitrogen™

Ambion™ RNase I efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues. RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. Supplied in one tube of 25,000 U (100 U/ µL).

FastAP Thermosensitive Alkaline Phosphatase (1 U/µL) Thermo Scientific™

Thermo Scientific FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.

TrueCut™ Cas9 Protein v2 Invitrogen™

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency. Features include: • Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in...

TURBO™ DNase (2 U/µL) Invitrogen™

TURBO™ DNase cleaves double-stranded DNA nonspecifically to leave 5' phosphorylated oligodeoxynucleotides. It has increased affinity for DNA-binding and remains active in the presence of salt. Note: this product is just the enzyme.

CpG Methyltransferase (M.SssI) Thermo Scientific™

Thermo Scientific M.SssI methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5’-CpG-3’ context. The enzyme is specifically formulated for fast reaction times without compromising the reaction efficiency.

S1 Nuclease Invitrogen™

S1 Nuclease is a single-strand-specific endonuclease that hydrolyzes single-stranded RNA or DNA into 5´ mononucleotides. The enzyme will hydrolyze single-stranded regions in duplex DNA such as loops and gaps. S1 Nuclease is stable at 65°C. Applications: Nuclease mapping techniques (1,2).

Proteinase K Solution Invitrogen™

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K Remains Active: • Over a wide pH range (optimal activity between 6.5 and 9.5) • Under denaturing conditions (e.g.