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Gallery Reagents for Food and Beverage Analysis Thermo Scientific™

Save time by eliminating reagent preparation with the Thermo Scientific™ Gallery™ system reagents for food and beverage analysis. Over 50 ready-to-use gallery system reagents are offered for food and beverage analysis, with the kits including two to four reagents in 20 mL barcoded vials. This enables the necessary identification data, such as lot numbers and expiration dates, to be read automatically by the discrete analyzer's barcode reader. Automated dilutions make it possible to apply methods for a wide range of analyte concentrations. Save time and reduce errors by simplifying your analysis with the Gallery system reagents.

TaqMan™ Array Human MicroRNA A Cards v2.0 Applied Biosystems™

The TaqMan® Array Human MicroRNA A Card v2.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool A v2.1 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool A v2.1 for situations where sensitivity is of the utmost importance and⁄or sample is limiting.

Axiom™ PharmacoFocus™ Assay Core Kit, mini 96-array Applied Biosystems™

The Axiom PharmacoFocus Assay Core Kit offers comprehensive coverage of targeted high-evidence pharmacogenomic research content (Pharmacogenomics Knowledge Base annotation levels of evidence 1A-2B) with a cost-efficient and high-throughput solution. The panel includes 2,000 variants in 150 genes and variants in regions of high homology to pseudo-genes (such as cytochrome P450 (CYP) genes), covers population diversity, and offers high accuracy and reproducibility.

The assay offers genotyping, copy number, and HLA typing, achieving the task of three different technologies in a single workflow at a cost per sample that enables profiling of hundreds to hundreds of thousands of samples. The assay is ideal for pharmacogenomic research in labs, academic hospitals, and health care centers, and for pharmaceutical companies with research applications in clinical trials.

Each Axiom PharmacoFocus Assay Core Kit, mini 96-array, includes all necessary array plates, reagents, controls, and consumables to process 94 samples and 2 controls. For a kit that offers star allele reporting with predicted gene phenotype, please see the Axiom PharmacoFocus Assay Kit, mini 96-array.

Features of the Axiom PharmacoFocus Assay Core Kit include:
• 2,000 markers in 150 genes of known pharmacogenomic value
• 88% of markers with PharmGKB annotation levels 1A-2B
• Accurate genotyping of markers in regions of high homology within CYP2C19, CYP2C8, CYP2D6, CYP2A6, CYP2B6, GSTM1, and SULT1A1
• Copy number variation (CNV) analysis for key ADME genes: CYP2D6, CYP2A6, GSTM1, GSTT1, UGB2B17, and SULT1A1
• HLA analysis with high resolution typing (2- and 4-digit) of the major histocompatibility complex (3 MHC Class I and 8 Class II loci) including HLA-A* and HLA-B* types with relevance in drug metabolism

The Axiom PharmacoFocus Assay Core Kit includes:
• Pharmacogenomic markers with highest PharmGKB levels of evidence (levels 1A, 1B, 2A, 2B) accessed in Nov 2019. Majority of annotations are associated with toxicity/adverse drug reactions and efficacy.
• Dutch Pharmacogenetics Working Group passport markers (accessed in Nov 2019)
• OpenArray TaqMan panels markers
• Markers referenced in the Center for Medicare and Medicaid (CMS) reimbursement policy including Psychiatry panel
• Human leukocyte antigen (HLA) markers associated with drug reactions

The Axiom PharmacoFocus Assay Core Kit requires the Applied Biosystems GeneTitan Multi-Channel Instrument, a platform used worldwide by genetic researchers and known for an efficient workflow and high reproducibility. Axiom arrays are manufactured using photolithographic manufacturing technology known for 100% fidelity, ensuring all markers are available in any manufacturing batch.

The Axiom PharmacoFocus Assay Core Kit data is analyzed with Applied Biosystems Axiom Analysis Suite software, which offers genotyping and copy number analysis. The Axiom HLA Analysis software can also be used for HLA typing.

Ion Xpress™ Barcode Adapters 1-16 Kit Ion Torrent™

The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.

Key Product Features:

• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Multiplexing with Barcode Adaptors Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.

Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.

Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.

For Research Use Only. Not for use in diagnostic procedures.

Countess™ Light Cube Removal Tool Thermo Scientific™

For use with Countess II FL and Countess 3 FL automated cell counters. Used for installation or removal of EVOS Light Cubes from the instruments.

ViralSEQ™ Vesivirus Detection Kit Applied Biosystems™

Viral contamination in mammalian product manufacturing presents a serious risk to the manufacturing process, the manufacturing facility, and the integrity of the product. Vesivirus 2117 is an RNA virus that has re-emerged as a potential threat to mammalian cell culture production. To meet the challenge of rapid, specific, and sensitive detection of Vesivirus, Applied Biosystems has developed the first commercially available integrated kit for isolation and detection of Vesivirus from mammalian cell culture samples.

The ViralSEQ™ Vesivirus Detection Assay is a PCR-based test that enables users to quickly isolate and detect Vesivirus 2117 and all other known strains with high sensitivity and specificity. The test utilizes PrepSEQ™ sample preparation magnetic beads to isolate viral RNA from a wide range of sample types, combined with TaqMan®-based technology for sensitive and specific detection of the viral RNA. The kit also includes a proprietary positive control template that helps eliminate the possibility of false positive results arising from inadvertent contamination of test samples with the positive control.
• Detection of known Vesivirus strains
• Rapid time-to-results in less than 5 hours
• TaqMan®-based real-time PCR assay
• Demonstrated sensitivity and specificity
• Proprietary optimized sample preparation for high RNA recovery

Table 1. Exclusion Panel.The ViralSEQ™ Assay has been demonstrated to be specific for Vesivirus and does not detect RNA purified from related and unrelated species and organisms.

Bacillus 2.5 ng Chicken 5 ng
Clostridium 2.5 ng Dog 5 ng
E. coli 2.5 ngHuman 5 ng
Lactoccus 2.5 ngMDCK 5 ng
Shigella 2.5 ngRat 5 ng
Yeast 5 ngCHO 500 ng
  Human RNA 1 µg

TaqMan™ Rodent MicroRNA A Array v2.0 Applied Biosystems™

The Applied Biosystems® TaqMan® Rodent MicroRNA A Array v2.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 335 and 226 unique microRNAs for mouse and rat respectively in as little as 5 hours. Three TaqMan® MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details of the included assays is provided in the Array Map file. Purchase of the Megaplex™ RT Primers, Rodent Pool A is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool A for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan® MicroRNA Arrays
provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan® MicroRNA Assays in parallel.

Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the the TaqMan® MicroRNA Array v2.0. When sensitivity is of the utmost importance, and/or sample is limiting Megaplex™ PreAmp Primers provide an optional preamplification step. TaqMan® MicroRNA Arrays v2.0 and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with Sanger miRBase v10 to be created in as little as 5 hours, providing the ideal miRNA profiling solution.

Simplify Sample Handling and Increase Sample Throughput
Following reverse transcription of miRNA targets using Megaplex RT Primers and optional preamplification using Megaplex PreAmp Primers, TaqMan® Universal PCR Master Mix is simply combined with each reaction and pipetted into each of the eight sample loading ports in the TaqMan® Array, simplifying sample handling and increasing sample throughput.

Provides Comprehensive Coverage of Sanger miRBase v10
Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan® MicroRNA Arrays v2.0. The content is derived from miRBase v10, and provides close to full coverage of Sanger with the most up-to-date TaqMan® MicroRNA Assays. Each array (either human or rodent) contains a selection of carefully chosen endogenous control assays, providing you with a variety of options for data normalization. Of particular note is the inclusion of a new mammalian U6 assay, repeated four times on each card, and an assay unrelated to mammalian species, ath-miR159a, providing a process control.

Ideal for miRNA Profiling
The highly streamlined workflow—coupled with the high specificity, sensitivity, and dynamic range of TaqMan® Assays—make this product solution ideal for miRNA profiling, especially when compared to microarrays.

GeneMapper™ ID-X Software v1.6, full installation Applied Biosystems™

This product includes a single GeneMapper ID-X Software v1.6 full installation (including database) and license.

GeneMapper ID-X Software is an automated genotyping solution for forensic casework and databasing data analysis, as well as for paternity data analysis. It supports a multi-user database configuration and provides a set of streamlined data analysis and sample review tools. It also offers a comprehensive quality value system that is designed to be optimized and validated for use in both expert system and traditional data analysis workflows. GeneMapper ID-X Software supports genotyping applications on the Applied Biosystems 310, 3100, 3130, 3130xl, 3500, 3500xl, 3730, and SeqStudio genetic analyzers. The release notes for this version provide information that should allow laboratories to decide whether internal validation testing is necessary for its system to comply with laboratory guidelines or regulations.

GeneMapper ID-X Software v1.6 features include:
• Supported operating systems: Windows® 7 Professional, 64-bit (Service Pack 1); Windows 10 Enterprise, 64-bit; Windows 10 Enterprise 2016 LTSB, 2019 LTSC
• Support for Java version 8
• Latest oracle security patches
• Support for CODIS 3.2 Revision 16 and CODIS 3.3 specifications
• Internationalized with local language support for English, Russian, and Chinese
• Enhanced command line interface functionality
• Enhanced user interfaces for better usability
• Enhanced printing
• View Table by Marker functionality in Report Manager and export as PDF
• Enhanced profile comparison
• Enhanced Security and Audit Module

 Overview of GeneMapper ID-X Software:
• Powerful, easy-to-use data analysis tool designed to increase lab productivity
• Comprehensive Expert System capability enables analysis of forensic databasing samples delivered by automated data assessment functionality and efficient manual review tools
• Complete Expert Assistant solution for analysis of forensic casework samples combines an efficient suite of manual review features with an integrated mixture analysis tool
• Quality control functionality allows rapid allele match comparisons and concordance searches within a data set
• Extensive security, auditing, and e-signature capabilities help protect data integrity and control technical records
• Multi-user database configuration stores your projects in a central location and facilitates information exchange
• Command Line Interface—offers the ability to access GeneMapper ID-X Software automatically (without human intervention) and helps streamline its integration with LIMS installations and other applications in an existing laboratory infrastructure

Automated data assessment
GeneMapper ID-X Software was designed to fulfill the requirements of both Expert System and Expert Assistant software. A combination of sophisticated automated data assessment processes and efficient manual review tools deliver comprehensive Expert System capability. This facilitates optimal interaction between the analyst and the data, helping to significantly reduce the amount of analysis time required for single source samples. Features such as the Analysis Requirements check, Allelic Ladder Quality Assessment, Improved Quality Value System, and Analysis Summary analyze samples and segregate them into pass or review categories based on your thresholds. Once segregated, you are enabled to easily conduct manual review of samples.

Despite its capabilities to automate and streamline the analysis of single source samples, an Expert System is unable to make the final analysis decision for most forensic casework samples and in particular for those containing mixtures. GeneMapper ID-X Software is designed to harness the automated, rule-based, subject-specific knowledge to act as an Expert Assistant to the forensic analyst, to help simplify much of the analysis process and empower the analyst to make key interpretation decisions. A mixture analysis tool determines the number of contributors to a mixture and helps efficiently manage the resulting possible genotypes. This enables the forensic analyst to interpret mixtures and generate accurate statistical calculations.

Manual tools help improve data review
Data analysis efficiencies offered by the automated data review processes have been further enhanced through the introduction of a suite of manual review tools. These developments focus on the plot window, enabling quick and easy access to all aspects of the data, thus helping to simplify the data review process. Colored marker header bars indicate which markers within a sample have failed to meet the user defined thresholds and the Process Quality Value (PQV) Details window details which flags have been fired and how much the observed value differs from the threshold. In addition, expanded labeling options are available to distinguish alleles from artifacts and reviewers can directly access raw data for further troubleshooting. Electronic/peer review of the data is enhanced with the review and manual acceptance of marker and sample level PQVs along with a detailed label edit table display.

For further troubleshooting, reviewers can also directly access raw data. The ability to review and manually accept markers and sample-level PQVs in a detailed label edit table display enhances electronic/peer data review.

A complete, verified system
GeneMapper ID-X Software is an integral component of the complete Thermo Fisher Scientific system of instruments, reagents, software, and services for human-identity applications. It has undergone extensive verification testing to help ensure robust design features, performance, and reproducibility. When you partner with Thermo Fisher Scientific, you gain access to one of the most extensive human identification resource networks in the industry. Our dedicated team of experienced forensic professionals—with extensive forensic product development experience—worked closely with the forensic community to develop GeneMapper ID-X Software. The software is part of a system that is supported by on-site technical assistance from skilled Field Applications Support Scientists and Field Service Engineers.

TaqMan™ Array, Human Signaling in Gap Junctions, Fast 96-well Applied Biosystems™

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Signaling In Gap Junctions 96-well Plate contains 92 assays to genes associated with signaling in gap junctions and 4 assays to candidate endogenous control genes.

NERL™ Trutol™ Glucose Tolerance Test Beverages Thermo Scientific™

Screen for diabetes mellitus, gestational diabetes and hypoglycemia with Thermo Scientific™ NERL™ Trutol™ Glucose Tolerance Test Beverages. Beverages are noncarbonated, caffeine-free and available in 50, 75 and 100g concentrations.

GeneChip™ Drosophila Genome 2.0 Array Applied Biosystems™

The GeneChip™ Drosophila Genome 2.0 Array is a microarray tool for studying expression of Drosophila melanogaster transcripts.

• Provides comprehensive coverage of the transcribed Drosophila genome on a single array
• Comprised of 18,880 probe sets, analyzing over 18,500 transcripts
• Sophisticated bioinformatics tools available through the NetAffx™ Analysis Center to rapidly obtain biologically meaningful results

Array Profile
Sequences used in the design of the GeneChip Drosophila Genome 2.0 Array were selected from Flybase version 3.1. Oligonucleotide probes complementary to each corresponding sequence are synthesized in situ on the arrays. Fourteen pairs of oligonucleotide probes are used to measure the level of transcription of each sequence.

Instrument Software Requirements
• GeneChip Scanner 3000, enabled for High-Resolution Scanning*
• GeneChip Operating Software (GCOS) v1.1.1, which contains the High-Resolution Scanning Update
*GeneChip Scanner 3000 High-Resolution Update is standard on all instruments shipped starting in September 2003 with serial number series 502. Previous versions, such as serial number series 501, will require the GeneChip Scanner 3000 High-Resolution Update, Catalog Number 00-0110, to be installed.

For more information, please review the data sheet (pdf, 700 KB).

TaqMan™ Array Human EGF Pathway Applied Biosystems™

The Applied Biosystems® TaqMan® Array Human EGF Pathway 96-well Plate contains 92 assays to EGF pathway associated genes and 4 assays to candidate endogenous control genes. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.

• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
• Fast delivery; on your bench in days
• Convenient; dried PCR primer and TaqMan® probe sets supplied in a 96- well plate; just add master mix and your sample
• Same TaqMan®Assay Quality, New Format

TaqMan® Array Plates contain the same high quality TaqMan® Gene Expression Assays (PCR primers and TaqMan® probe sets) that have been commercially available for years in a convenient, dried, 96-well format. Extensive tests have found no difference in performance between TaqMan®Assays supplied in the traditional liquid format and assays dried into a TaqMan® Array Plate. Figure 1 shows the results of an experiment comparing the linear range of the two formats, where no difference in performance was observed.

Just Add Master Mix and Sample, and Begin Cycling
Ideal for projects such as validation of microarray and RNAi data, and pathway studies, the plates include just the right amount of TaqMan®Assay in each well: enough for one 20 µl reaction. You can order as few as three Gene Signature Plates, making these an affordable option for many laboratories and minimizing the waste of unused individually purchased TaqMan®Assays. Save a significant amount of time setting up your experiments. Just add master mix and sample, and begin cycling.

Figure 2 is an example Plate Layout with Gene Symbols and Assay IDs

Plate Map Ensures Proper Data Labeling and Easy Re-Ordering
Each pack of TaqMan® Array Plates is shipped with a CD containing the plate configuration map (see Figure 2) along with the details of each TaqMan®Assay. This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering.

Simple to Use
Each TaqMan® Array Plate well contains a single TaqMan®Assay for one 20 µl reaction. The plates can be stored at room temperature or at 4°C. When you are ready to run your assays, simply add PCR master mix and your cDNA sample, seal the plate, and begin cycling on a real-time PCR instrument.

Compatibility
TaqMan® Array Plates have been optimized for use with TaqMan® Gene Expression Master Mix, but are also compatible with TaqMan® Universal PCR Master Mix. For studies using cultured cells, TaqMan® Array Plates are also compatible (and recommended) with the TaqMan® Gene Expression Cells-to-Ct™ Kit. TaqMan® Array Plates must be run on a real-time PCR instrument with 96-well plate capability. The plates have been validated for use on Applied Biosystems® 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20 µl reaction

GeneMapper™ ID-X Software v1.6, client installation Applied Biosystems™

This product includes a single GeneMapper ID-X Software v1.6 client installation (no database) and five licenses.

GeneMapper ID-X Software is an automated genotyping solution for forensic casework and databasing data analysis, as well as for paternity data analysis. It supports a multi-user database configuration and provides a set of streamlined data analysis and sample review tools. It also offers a comprehensive quality value system that is designed to be optimized and validated for use in both expert system and traditional data analysis workflows. GeneMapper ID-X Software supports genotyping applications on the Applied Biosystems 310, 3100, 3130, 3130xl, 3500, 3500xl, 3730, and SeqStudio genetic analyzers. The release notes for this version provide information that should allow laboratories to decide whether internal validation testing is necessary for its system to comply with laboratory guidelines or regulations.

GeneMapper ID-X Software v1.6 features include:
• Supported operating systems: Windows® 7 Professional, 64-bit (Service Pack 1); Windows 10 Enterprise, 64-bit; Windows 10 Enterprise 2016 LTSB
• Support for Java version 8
• Latest oracle security patches
• Support for CODIS 3.2 Revision 16 and CODIS 3.3 specifications
• Internationalized with local language support for English and Chinese
• Enhanced command line interface functionality
• Enhanced user interfaces for better usability
• Enhanced printing
• View Table by Marker functionality in Report Manager and export as PDF
• Enhanced profile comparison
• Enhanced Security and Audit Module

 Overview of GeneMapper ID-X Software:
• Powerful, easy-to-use data analysis tool designed to increase lab productivity
• Comprehensive Expert System capability enables analysis of forensic databasing samples delivered by automated data assessment functionality and efficient manual review tools
• Complete Expert Assistant solution for analysis of forensic casework samples combines an efficient suite of manual review features with an integrated mixture analysis tool
• Quality control functionality allows rapid allele match comparisons and concordance searches within a data set
• Extensive security, auditing, and e-signature capabilities help protect data integrity and control technical records
• Multi-user database configuration stores your projects in a central location and facilitates information exchange
• Command Line Interface—offers the ability to access GeneMapper ID-X Software automatically (without human intervention) and helps streamline its integration with LIMS installations and other applications in an existing laboratory infrastructure

Automated data assessment
GeneMapper ID-X Software was designed to fulfill the requirements of both Expert System and Expert Assistant software. A combination of sophisticated automated data assessment processes and efficient manual review tools deliver comprehensive Expert System capability. This facilitates optimal interaction between the analyst and the data, helping to significantly reduce the amount of analysis time required for single source samples. Features such as the Analysis Requirements check, Allelic Ladder Quality Assessment, Improved Quality Value System, and Analysis Summary analyze samples and segregate them into pass or review categories based on your thresholds. Once segregated, you are enabled to easily conduct manual review of samples.

Despite its capabilities to automate and streamline the analysis of single source samples, an Expert System is unable to make the final analysis decision for most forensic casework samples and in particular for those containing mixtures. GeneMapper ID-X Software is designed to harness the automated, rule-based, subject-specific knowledge to act as an Expert Assistant to the forensic analyst, to help simplify much of the analysis process and empower the analyst to make key interpretation decisions. A mixture analysis tool determines the number of contributors to a mixture and helps efficiently manage the resulting possible genotypes. This enables the forensic analyst to interpret mixtures and generate accurate statistical calculations.

Manual tools help improve data review
Data analysis efficiencies offered by the automated data review processes have been further enhanced through the introduction of a suite of manual review tools. These developments focus on the plot window, enabling quick and easy access to all aspects of the data, thus helping to simplify the data review process. Colored marker header bars indicate which markers within a sample have failed to meet the user defined thresholds and the Process Quality Value (PQV) Details window details which flags have been fired and how much the observed value differs from the threshold. In addition, expanded labeling options are available to distinguish alleles from artifacts and reviewers can directly access raw data for further troubleshooting. Electronic/peer review of the data is enhanced with the review and manual acceptance of marker and sample level PQVs along with a detailed label edit table display.

For further troubleshooting, reviewers can also directly access raw data. The ability to review and manually accept markers and sample-level PQVs in a detailed label edit table display enhances electronic/peer data review.

A complete, verified system
GeneMapper ID-X Software is an integral component of the complete Thermo Fisher Scientific system of instruments, reagents, software, and services for human-identity applications. It has undergone extensive verification testing to help ensure robust design features, performance, and reproducibility. When you partner with Thermo Fisher Scientific, you gain access to one of the most extensive human identification resource networks in the industry. Our dedicated team of experienced forensic professionals—with extensive forensic product development experience—worked closely with the forensic community to develop GeneMapper ID-X Software. The software is part of a system that is supported by on-site technical assistance from skilled Field Applications Support Scientists and Field Service Engineers.

Oncomine™ Comprehensive Assay v3M Ion Torrent™

The Oncomine Comprehensive Assay v3 is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 161 unique genes to help inform drug discovery research and clinical trial research programs. It provides the reagents for library construction and four pools—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Designed to help you go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion S5 System with the Ion 540 Chip to enable up to 8 samples (7 samples and one no-template control) per run.

When combined with Oncomine Knowledge Reporter Software, you can create reports that align labels, guidelines, and clinical trials to your results so you can focus on key drivers of cancer in your research.

Key features of the assay are:
• Enables analysis of variants across 161 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool (40 ng total) isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

From sample to answer
The Oncomine Comprehensive Assay is part of an NGS workflow that helps researchers to filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

The workflow has been adopted by large-scale national clinical trial research programs including NCI-MATCH (United States) and LC-SCRUM (Japan), and includes:
• Proven Ion AmpliSeq chemistry
• Automated library and template prep on the Ion Chef System
• Scalable sequencing on the Ion S5 Sytem
• Optimized Oncomine informatics

Multiple configurations
The Oncomine Comprehensive Assay v3 comes in the following configurations to match your laboratory needs:

Manual library preparation configuration:
Oncomine Comprehensive Library Assay v3M
This configuration contains all the reagents needed to prepare libraries for 24 or 96 samples:
    • Oncomine Comprehensive Panel v3M DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3M RNA—contains the RNA primer pools
    • Ampliseq Library Plus reagents—allow you to manually create your library
Ion Chef automated library preparation configuration:
Oncomine Comprehensive Library Assay v3C
This configuration contains all the reagents needed to prepare libraries for 32 samples:
    • Oncomine Comprehensive Panel v3C DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3C RNA—contains the RNA primer pools
    • Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System


TaqMan™ Array, Human NFAT & Cardiac Hypertrophy, Fast 96-well Applied Biosystems™

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human NFAT & Cardiac Hypertrophy 96-well Plate contains 92 assays to NFAT & cardiac hypertrophy associated genes and 4 assays to candidate endogenous control genes.
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