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Reagent Type


RNAi Type


Maybridge HitFinder Screening Library Maybridge™

Greater than 14,000 compounds pre-plated at 1μmol in 96-well plates

GeneArt™ Plasmid Premium Prep, E.coli fermentation, H2O Thermo Scientific™

The GeneArt® Gene Synthesis service offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence.

ClaSeek Library Preparation Kit, Illumina compatible Thermo Scientific™

Thermo Scientific ClaSeek Library Preparation Kit, Illumina™ compatible, is designed for fast and convenient construction of PCR-free NGS fragment library from DNA sample input as low as 500 ng. In combination with the amplification protocol the kit also enables the generation of DNA fragment libraries from ultra-low 5 ng sample input.

The kit utilizes a highly efficient NGS-optimized library construction protocol combining end-conversion and adapter addition steps into a simple and convenient one-tube procedure. This minimizes unnecessary pipetting steps and reduces hands-on time, allowing for PCR-free library construction in less than 70 minutes. The kit is suitable for construction of 50, 300, 500 bp read-length Illumina™-compatible libraries and is validated for use on Illumina™ HiSeq™ and MiSeq™ sequencing platforms.

Highlights

PCR-free—significantly minimized bias and improved coverage with amplification-free protocol
Flexible—universal protocol for variable sample input (5 ng—1 µg) and DNA fragment lengths
Fast and Convenient—fewer cleanup steps, less hands-on time, optimized master mixes, one-tube end conversion procedure.

Applications

DNA fragment library preparation for downstream use in next generation sequencing workflow.

Related Products
ClaSeek Library Preparation Kit, Illumina compatible

Custom RNA, 5' PHO Invitrogen™

Use this tool for fast and easy online ordering of bench-tested Validated Stealth RNAi™ siRNA, ready-to-order Stealth Select RNAi™ siRNA, bench-tested BLOCK-iT™ Pol II miR Validated miRNA Vector Duopaks, and ready-to-clone BLOCK-iT™ miR RNAi Select.

Custom RNA, HPLC Invitrogen™

Use this tool for fast and easy online ordering of bench-tested Validated Stealth RNAi™ siRNA, ready-to-order Stealth Select RNAi™ siRNA, bench-tested BLOCK-iT™ Pol II miR Validated miRNA Vector Duopaks, and ready-to-clone BLOCK-iT™ miR RNAi Select.

Mouse BAC DNA Library Pool Thermo Scientific™

Mouse Bacterial Artificial Chromosome (BAC) Library Pools are supplied as polymerase chain reaction (PCR) ready plasmid DNA arranged in an easy to screen format. There is no need to store a large library or to prepare plasmids.

GeneArt™ Plasmid Premium Prep, E.coli fermentation, PBS Thermo Scientific™

The GeneArt® Gene Synthesis service offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence.

GeneArt™ Plasmid Premium Prep (high copy plasmid), H2O (Ready-to-Work Package) Thermo Scientific™

The GeneArt® Gene Synthesis service offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence.

Custom RNA, HPLC Invitrogen™

Use this tool for fast and easy online ordering of bench-tested Validated Stealth RNAi™ siRNA, ready-to-order Stealth Select RNAi™ siRNA, bench-tested BLOCK-iT™ Pol II miR Validated miRNA Vector Duopaks, and ready-to-clone BLOCK-iT™ miR RNAi Select.

Custom RNA, Internal DNA Modification Invitrogen™

Use this tool for fast and easy online ordering of bench-tested Validated Stealth RNAi™ siRNA, ready-to-order Stealth Select RNAi™ siRNA, bench-tested BLOCK-iT™ Pol II miR Validated miRNA Vector Duopaks, and ready-to-clone BLOCK-iT™ miR RNAi Select.

Custom RNA, Stealth Selectect Set Seq Invitrogen™

Using our award-winning BLOCK-iT™ RNAi Designer, you can design Custom BLOCK-iT™ siRNA sequences for efficient and specific gene expression knockdowns in any organism. For enhanced target specificity and stability in serum, Stealth RNAi™ RNAi chemistry is available.

Custom RNA, HPLC Invitrogen™

Use this tool for fast and easy online ordering of bench-tested Validated Stealth RNAi™ siRNA, ready-to-order Stealth Select RNAi™ siRNA, bench-tested BLOCK-iT™ Pol II miR Validated miRNA Vector Duopaks, and ready-to-clone BLOCK-iT™ miR RNAi Select.

Freedom™ CHO-S™ Kit Gibco™

Gibco® Freedom® CHO-S® Kit, co-developed with ProBioGen AG, is an easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in Chinese Hamster Ovary (CHO)-derived suspension culture. The Gibco® Freedom® CHO-S® Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit. The Gibco® Freedom® CHO-S® Kit also delivers high titers, and offers simplified commercial licensing options.

• Flexible commercial licensing without the need for royalties
• Ability to achieve IgG titers over 3g/L
• Complete workflow that can take your gene of interest from transfection to lead clone typically in 5 months
• Convenient packaging, for simplified ordering and storage

Commercial Licensing that is Simple, Flexible and Without Royalties
Multiple companies provide CHO (Chinese Hamster Ovary) stable cell line development services and products that require annual fees during development of your therapeutic protein. In addition, royalties may be required on the sales of your therapeutic protein upon commercialization. Gibco® Freedom® Kits provide you with the tools that enable you or your CRO provider to develop your stable cell line for just the price of the kit itself. Furthermore, as you move towards commercial use, a flexible license structure is available without the burden of royalties and works to keep it as simple as a onetime payment (Fig 1). For more information regarding licensing, email us at outlicensing@lifetech.com.

Industry Standard Production Levels
Current bioprocess industry standards to achieve manufacturing efficiencies for IgG type molecule production with stable CHO cell lines in bioreactors is 2-3g/L or better. We have demonstrated that the Gibco® Freedom® CHO-S® Kit can consistently generate IgG producing scalable clones (stable out to 60 population doublings), that meet this standard of productivity (Fig 2).

Complete, Fast, and Efficient Workflow
The Gibco® Freedom® CHO-S® Kit’s complete, proven, and fully integrated workflow allows you to move from transfection to lead clone typically in 5 months, with reduced hands on time, and reduced Fulltime Equivalent (FTE) requirements (Fig 3). The entire workflow is performed in our CD FortiCHO™ medium, a chemically defined, animal origin free (AOF) medium. The kit also offers the following advantages:

• Cloning of one or two genes that encode your protein(s) of interest
• High efficiency transfection of DNA into CHO-S® Cells (cGMP-banked)
• Included flash drive with loaded manual

New Convenient Packaging
To free you to focus on the creation of your stable cell line rather than on figuring out where and when all the components of your experiment are arriving, Freedom® CHO-S® Kit introduces the smART™ tote packaging system (shown in above product photos). All components for the creation of your expression cell line are delivered in soft cooler totes along with a parts flyer, and a flash drive containing the manual. Upon delivery you have all the information needed for correct storage and use of components consolidated in the two smART™ tote shipment coolers.

Access to Gibco® Freedom® Kit Support
If any specific support is needed beyond the manual, please contact your account manager to obtain a Process Science Manager consultation. In addition, one of our support scientists may check in with you to ensure that your experience with the kit is as successful as possible. For further assistance, please email our support team at GibcoServices@thermofisher.com.

XTRA MM BAC SUPERPL DNA Thermo Scientific™

XTRA MM BAC SUPERPL DNA

GeneArt™ Plasmid Premium Prep (high copy plasmid), PBS (Ready-to-Work Package) Thermo Scientific™

The GeneArt® Gene Synthesis service offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence.
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