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Qubit™ ssDNA Assay Kit Invitrogen™

The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for ssDNA quantitation?
• For 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

BloodPrep™ DNA Purification Solution Invitrogen™

The BloodPrep® DNA Purification Solution is a cell lysis and DNA purification solution for whole blood from any animal species, cultured cells, and buccal swabs for use with BloodPrep® DNA chemistry.

For Research Use Only. Not for use in diagnostics procedures.

pcDNA™3.2-DEST Mammalian Expression Vector Invitrogen™

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features:

•Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
•C-terminal V5 tag for easy detection
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer.

Quant-iT™ RNA Assay Kit, broad range Invitrogen™

The new Quant-iT™ RNA Assay Kit, Broad Range, provides an accurate and selective method for quantitation of high-abundance RNA samples. Whereas the original Quant-iT™ RNA Kit has great sensitivity, the Quant-iT™ RNA Broad Range Kit is ideal for microarray samples where the concentration of RNA is higher. With the Quant-iT™ RNA Broad Range Kit, dilution of the RNA samples prior to quantitation is not required. The kit is specific for RNA and will not quantitate DNA, protein or free nucleotides.

Tempus™ Blood RNA Tube Applied Biosystems™

Tempus™ Blood RNA Tubes are for stabilization and isolation of total RNA from whole blood for gene expression analysis. Advantages of Tempus™ Blood RNA Tubes:

• Immediately lyse whole blood cells and stabilize RNA in a single step—no RBC lysis or proteinase K treatments required
• Freeze global gene expression profiles for up to five days at room temperature; greater than seven days at 4°C

How it works
Each Tempus™ Blood RNA Tube contains 6 mL Stabilizing Reagent. When the blood (3 mL) is drawn into the tube and mixed with this reagent, lysis occurs almost immediately. The stabilizing reagent inactivates cellular RNases and selectively precipitates RNA; genomic DNA (gDNA) while proteins remain in solution. Blood drawn into the Tempus™ Blood RNA Tube is stable for up to five days at room temperature, or minimally seven days at 4°C.

Accessory product
When the Tempus™ Blood RNA Tubes are used with the Tempus™ 12-Port RNA Isolation Kit (Cat. No. 4378672), RNAs with greater stability and higher yields of RNA are produced (see figure).

Homogenizer Invitrogen™

The Homogenizer is for use with the Ambion™ PureLink™ RNA Mini Kit (Cat. Nos. 12183020, 12183018A, 12183025) to reduce the viscosity of difficult tissue samples between 5 and 30 mg during RNA isolation.

DreamTaq™ Hot Start DNA Polymerase Thermo Scientific™

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq buffer containing magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available with pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.

DreamTaq Hot Start DNA Polymerase is formulated to enhance productivity through:
• Better reaction outcomes
   --Higher yield of target amplicons from low template amounts
   --Increased reaction specificity due to reliable hot-start technology
   --Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6 kb
• Enhanced convenience
   --Room temperature reaction set-up
   --Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer

Applications:
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning

More DreamTaq Hot Start DNA Polymerase products >

RNA Gel Loading Dye (2X) Thermo Scientific™

Thermo Scientific 2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide. In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA.

2X RNA Loading Dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilizes RNA.

Highlights

• Two-color tracking of DNA, RNA migration during electrophoresis
• No DNA, RNA masking during gel exposure to UV light
• Denaturation of DNA, RNA due to the presence of formamide

Applications

• Preparation of DNA ladders, markers, and samples for loading on denaturing gels
• Preparation of RNA ladders, markers, and samples for loading on agarose or polyacrylamide gels

Polypropylene Columns (Empty) Invitrogen™

These empty, 10-mL polypropylene columns are for use with the ProBond™ Purification System and hold ProBond™ resin.

pcDNA™6.2-DEST Mammalian Expression Vector Invitrogen™

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-DEST vector offers the following key features:

•Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
•C-terminal V5 tag for easy detection
•Blasticidin resistance gene for efficient stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer.

DES™-Inducible/Secreted Kit with pCoBlast Invitrogen™

The DES®-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:

• Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.

Agarose I (Molecular Biology Grade) Thermo Scientific™

Thermo Scientific Pierce Agaroses are highly purified and carefully blended formulations of regular- or low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.

Features:

• A regular melting temperature agarose for electrophoresis requiring an exceptional level of purity with unequaled performance

Related Products
Agarose II (Low Melt)

TRIzol™ Plus RNA Purification Kit Invitrogen™

The TRIzol® Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol® Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink® RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.

Features of this kit include:

• Combined TRIzol®-based lysis and PureLink™ spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol

Two products in one
The TRIzol® Plus RNA Purification System couples the lysis capabilities of TRIzol® Reagent with the 1000 µg column-;binding capacity of the PureLink™ RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol® Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol® Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol® Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.

Simple lysis to column purification protocol
TRIzol® Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol® Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink™ RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).

Gateway™ pcDNA™-DEST40 Vector Invitrogen™

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway® pcDNA™-DEST40 vector offers the following key features:

•C-terminal V5-6x His tag for easy detection and rapid purification
•Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer.

GeneJET Plasmid Miniprep Kit Thermo Scientific™

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.

Highlights

Efficient—high yields of up to 20 µg of high quality plasmid DNA
Fast—procedure takes less than 14 minutes
Convenient—no phenol/chloroform extraction or alcohol precipitation required
Pure—purified DNA is immediately ready to use

Applications

• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
In vitro transcription
• Transformation

Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol

Related Products
RNase A
Elution Buffer for GeneJET Plasmid Miniprep Kit
Lysis Solution for GeneJET Plasmid Miniprep Kit
Resuspension Solution for GeneJET Plasmid Miniprep Kit
Neutralization Solution for GeneJET Plasmid Miniprep Kit
Wash Solution for GeneJET Plasmid Miniprep Kit
Binding Buffer for GeneJET Plasmid Miniprep Kit
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