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Suitable Downstream Analysis


PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit Invitrogen™

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 90 minutes, purify up to 1.5 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Advantages of the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit include:
• Simple and fast protocol—purification typically takes 90 minutes
• High yield—isolate up to 1.5 mg of high quality plasmid DNA
• Endotoxin-free plasmid—<0.1 EU/µg, ideal for sensitive applications
• High purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
• Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin

Simple and fast protocol
Unlike other anion-exchange-based endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification. There is no waiting for gravity-flow columns and the entire purification typically takes 90 minutes (including the final precipitation step).

Endotoxin-free plasmid
Purify endotoxin-free (<0.1 EU/µg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines in vivo.

Compatible with ExpiCHO and Expi293 expression systems
Plasmid purified using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit is fully compatible with the Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Superior quality with endotoxin-free components
Rest assured that the kit is of high quality and has additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind.

PureLink™ Expi Endotoxin-Free Buffer Set Invitrogen™

The PureLink Expi Endotoxin-Free Buffer Set provides the same buffers included in the PureLink Expi Endotoxin-Free plasmid purification kits, made available separately here for those applications that would benefit from additional buffers. The Buffer Set includes all buffers and endotoxin-free components needed to enable 30 Maxi, 4 Mega, or 2 Giga PureLink Expi Endotoxin-Free plasmid purifications. Specifically, the buffer set includes RNAse A, resuspension buffer (R3), lysis buffer (L7), precipitation buffer (N3), equilibration buffer (EQ1), wash buffer (W8), elution buffer (E4), TE buffer (TE), endotoxin removal buffer, lysis indicator, and endotoxin-free water.

Ni-NTA Purification System Invitrogen™

The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different conditions.

SUPERase•In™ RNase Inhibitor (20 U/μL) Invitrogen™

SUPERase•In RNase Inhibitor is a protein-based inhibitor of non-human origin that noncovalently binds and inhibits the most common and troublesome RNases, including RNase A, B, C, 1, and T1.

SUPERase•In RNase Inhibitor can be used in any application where RNase contamination could be problematic. Because it inhibits a broader range of RNases than traditional RNase inhibitors, SUPERase•In is the most effective RNase inhibitor available, providing a higher level of protection against degradation.

SUPERase•In does not interfere with other enzymes such as RNA polymerases, reverse transcriptase, or Taq DNA polymerase. Additionally, SUPERase•In is active up to 65°C and over a pH range of 5.5 to 8.5.

If you are plannning to use this product with standard antibody purification methods, please contact our technical support to discuss experimental design.

Unit definition
SUPERase•In RNase Inhibitor at 1 U/µL will block the degradation of 0.1 µg/µL labeled RNA by 2.5 pg/µL of RNase A, 2.5 pg/µL of RNase I, and 0.0075 U/µL of RNase T1, for 4 hours at 37°C, in 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM EDTA. Analysis is by denaturing PAGE. SUPERase•In is currently the only ribonuclease inhibitor for which the unit activity is defined by such a functional assay.

GeneTitan™ Hybridization Module for miRNA Plates Applied Biosystems™

The GeneTitan™ Hybridization Module for miRNA Plates is used with the miRNA 3.1 and 4.1 Array Plates.

Breast Adenocarcinoma (MCF-7) Total RNA Invitrogen™

The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any downstream application, including RT-PCR. Note: While it is impossible to remove every DNA molecule from an RNA sample, each preparation is tested to ensure that any residual DNA contamination is insignificant. The integrity of the RNA is verified by capillary electrophoresis using the Agilent® 2100 bioanalyzer.

DNA Extract All Reagents Kit Applied Biosystems™

The DNA Extract All Reagents Kit provides PCR-ready DNA from a wide variety of sample types, ranging from blood to buccal swabs to plant tissues in 5 minutes.

Simply mix your sample with the DNA lysis solution provided. Incubate for 3 minutes and add the DNA stabilizing solution, also provided.

After extraction, robust PCR amplification can be completed using TaqMan® GTXpress™ Master Mix or TaqMan® OpenArray® Genotyping Master Mix (pre-amplified sample only), depending on your application. PCR can be performed using either Fast or Standard mode thermal cycling conditions. The DNA Extract All Reagents Kit is included in the TaqMan Sample-to-SNP Kit, so workflow details can be found in the TaqMan® Sample-to-SNP user guide below.

This 5 mL size should be sufficient for 100 extractions.

Note: Thermal cycling conditions should be followed according to the master mix being used. TaqMan®™ GTXpress™ Master Mix is required for PCR amplification of sample prepared using the DNA Extract All Reagents Kit. If sample has undergone preamplification, OpenArray® Genotyping Master Mix may also be used.

PureLink™ Pro 96 total RNA Purification Kit Invitrogen™

The PureLink® Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink® Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Microplates for Fluorescence-based Assays, 96-well Invitrogen™

Molecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. These plates are compatible with standard fluorescence microplate readers and are offered in a convenient 10-pack size for those times when only a few assay plates are required.

Cervical Adenocarcinoma (HeLa-S3) Total RNA Invitrogen™

The highest-quality RNA available from Ambion®, it is DNase-treated and subjected to unsurpassed quality control standards. One tube containing 100 µg is provided at a concentration of 1 mg/mL. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects used to judge the quality of FirstChoice® RNAs. The samples are processed using Ambion® RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any downstream application, including RT-PCR. Note: While it is impossible to remove every DNA molecule from an RNA sample, each preparation is tested to ensure that any residual DNA contamination is insignificant. The integrity of the RNA is verified by capillary electrophoresis using the Agilent® 2100 bioanalyzer.

PureLink™ Air Porous Tape Invitrogen™

The PureLink® Air Porous Tape is a component in the PureLink® 96 Plasmid Purification System. The PureLink® 96 Plasmid Purification offers a method for high throughput plasmid DNA isolation. The PureLink® Air Porous Tape allows for proper aeration of bacterial cultures.

DreamTaq™ Hot Start Green DNA Polymerase Thermo Scientific™

Thermo Scientific™ DreamTaq™ Hot Start Green DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq Green buffer that includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel. The buffer contains magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available without pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.

DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

DreamTaq Hot Start Green DNA Polymerase is formulated to enhance productivity through:
• Better reaction outcomes
   --Higher yield of target amplicons from low template amounts
   --Increased reaction specificity due to reliable hot-start technology
   --Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6 kb
• Enhanced convenience
   --Room temperature reaction set-up
   --Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
   --Direct loading of PCR products on a gel with DreamTaq Green buffer

Applications:
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning

More DreamTaq Hot Start DNA Polymerase products >

Neutralization Solution Thermo Scientific™

Thermo Scientific GeneJET Neutralization Solution is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 240 mL aliquots.

RNaseAlert™ QC System v2 Invitrogen™

The RNaseAlert® QC System v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This version contains a substrate that is ~60% more sensitive than the previous version. The kit contains sufficient reagents for 5 x 96 (480) high-throughput assays.

Features of RnaseAlert® QC System v2:

• Detects as little as ~0.3 pg of RNase A with a fluorometer
• Simple, straight-forward, 30-minute assay
• Designed to monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample

The RNaseAlert® QC System v2 uses a new, more sensitive, novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.

Fluorescence can be readily detected with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution for a few minutes. The results are then read on a fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

Accessory Product
The RNaseAlert® QC System v2 is fully compatible with the DNaseAlert™ QC System. Since the DNaseAlert™ substrate contains a fluorescent tag that is spectrally distinct from RNaseAlert® substrate, the two kits can be used simultaneously for quantitative real-time analyses of both RNases and DNases within a single sample.

NUCLEIC-CARD™ COLOR matrix, 4 spots Thermo Scientific™

NUCLEIC-CARD COLOR is an easy-to-use system for collection, preservation, and long-term storage of nucleic acids at room temperature. The NUCLEIC-CARD COLOR matrix is chemically-treated to enable cell lysis and protein denaturation. Nucleic acids are immobilized and preserved for long-term storage at room temperature. NUCLEIC-CARD COLOR changes color upon sample application to facilitate processing of buccal samples. NUCLEIC-CARD collection devices are manufactured by Copan, a market leader in the area of sample collection tools, and distributed globally by Thermo Fisher Scientific.

Key Features:

• Enables direct PCR amplification from a card punch, eliminating extraction steps
• Facilitates high quality STR profiles with Applied Biosystems direct PCR amplification kits
• Certified free of DNase, Rnase, and amplifiable human DNA
• Prevents growth of microorganisms to enable long-term room-temperature storage
• Easily automated with commercially-available punching systems to minimize manual handling and maximize sample integrity

For Forensic or Paternity Use Only.

NUCLEIC-CARD is a trademark of Copan Flock Technologies.
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