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DNA Vectors
DNA vectors are molecules used as a vehicle to transfer foreign genetic material into a host cell where it can be replicated and/or expressed. These products include kits for cloning, transfection, and expression of DNA.
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pcDNA™3.1 (+) Mammalian Expression Vector Invitrogen™
Designed for high-level, constitutive expression in a variety of mammalian cell lines
Gateway™ pDEST™17 Vector Invitrogen™
Destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins
pLenti6/V5-DEST™ Gateway™ Vector Invitrogen™
For lentiviral-based expression of a target gene in dividing and nondividing mammalian cells
pcDNA™6.2-DEST Mammalian Expression Vector Invitrogen™
Designed for high-level, constitutive expression in variety of mammalian cell lines
pcDNA™3.1 (-) Mammalian Expression Vector Invitrogen™
Designed for high-level, constitutive expression in a variety of mammalian cell lines
pPICZα A, B, & C Pichia Vectors Invitrogen™
Allows high-level, methanol inducible expression of the gene of interest in Pichia, and can be used in Pichia strains including X-33, SMD1168H, and KM71H
pPICZ A, B, & C Pichia Vectors Invitrogen™
Designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein
pcDNA™3.3-TOPO™ TA Cloning™ Kit Invitrogen™
Designed to deliver exceptionally high levels of transgene expression in adherent cells
pYES2 Yeast Expression Vector Invitrogen™
For native expression of the protein of interest in S. cerevisia
pLenti7.3/V5-DEST™ Gateway™ Vector Kit Invitrogen™
The pLenti7.3/V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti7.3/V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells.
Contain a strong promoter for high-level expression in mammalian cells
pCEP4 Mammalian Expression Vector Invitrogen™
Designed for high-level, constitutive expression from either the CMV or RSV promoters
pJTI™ R4 CMV-TO MCS pA Vector
Gateway™ pEF-DEST51 Vector Invitrogen™
attR sites for recombination with any attL-flanked fragment, regardless of whether it is entry clone or Ultimate™ RF Clone
Plasmid pCMV·SPORT-βgal Invitrogen™
Used as a positive control for monitoring expression in eukaryotic cells
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Molecular cloning, a key component of the molecular biology workflow, is used to assemble recombinant DNA molecules and to direct their replication. In the molecular cloning workflow, the DNA to be cloned is identified and treated with enzymes to generate DNA fragments.
Lentiviral vectors are a widely used modality for gene modification in cell therapy manufacturing processes. The innovative lentivirus production solutions from Thermo Fisher Scientific are designed to accelerate and streamline the development of gene-modified cell therapies and gene therapies.
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I am interested in using the CRISPR system to modify my gene of interest using HDR instead of NHEJ repair. How can I do this?
Do I need to use the P3000 Reagent when transfecting siRNA? What if I am transfecting shRNA or miRNA in a vector?