DNA Vectors

DNA Vectors

DNA vectors are molecules used as a vehicle to transfer foreign genetic material into a host cell where it can be replicated and/or expressed. These products include kits for cloning, transfection, and expression of DNA.
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pcDNA™3.1/V5-His TOPO™ TA Expression Kit Invitrogen™

The pcDNA™3.1/V5-His TOPO™ TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO™ vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants.

DES™-Blasticidin Support Kit Invitrogen™

The DES™-Blasticidin Support Kit is designed to be paired with any DES™ Expression vector to allow rapid selection of stable S2 cells with Blasticidin. The DES™-Blasticidin Support Kit contains the pCoBlast selection vector for rapid generation of stable S2 cell lines with Blasticidin.

pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli Invitrogen™

The pENTR™ 5’-TOPO™ TA Cloning™ Kit combines Invitrogen’s TOPO™ Cloning and MultiSite Gateway™ Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway™ entry vector with ≥ 85% efficiency.

Flp-In™ T-REx™ Core Kit Invitrogen™

The Flp-In™ T-REx™ System allows the generation of stable mammalian cell lines exhibiting tetracycline-inducible expression of a gene of interest from a specific genomic location. Features of the Flp-In™ T-REx™ System include: • Once the Flp-In™ T-REx™ host cell line containing an integrated FRT...

pcDNA™3.3-TOPO™ TA Cloning™ Kit Invitrogen™

The pcDNA™3.3-TOPO™ TA Cloning™ Kit is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.

ViraPower™ Lentiviral Gateway™ Expression Kit Invitrogen™

The ViraPower™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and...

pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli Invitrogen™

The pCR™8/GW/TOPO™ TA Cloning Kit delivers fast, efficient and reliable cloning. Additionally, this vector is ideal for use in Gateway™ recombination cloning into various destination vectors for a multitude of applications.

pBAD202 Directional TOPO™ Expression Kit Invitrogen™

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence.

pTrcHis TOPO™ TA Expression Kit Invitrogen™

The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO™ or pTrcHis2-TOPO™, includes a minicistron element for enhanced...

ViraPower™ UbC Lentiviral Gateway™ Expression Kit Invitrogen™

The ViraPower™ UbC Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen™’s ViraPower™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning...

NT-GFP Fusion TOPO™ Expression Kit Invitrogen™

The GFP Fusion TOPO™ TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle 3 GFP protein. These kits provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO™ or pcDNA3.1/CT-GFP-TOPO™ vectors (Figure 1).

ViraPower™ Lentiviral Directional TOPO™ Expression Kit Invitrogen™

The ViraPower™ Lentiviral Directional TOPO™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO™ technologies to facilitate quick PCR-based cloning...

ViraPower™ Bsd Lentiviral Support Kit Invitrogen™

The ViraPower™ Bsd Lentiviral Support Kit contains the necessary components to transfect 293FT cells to generate viral particles containing the target gene. The kit contains the ViraPower™ Lentiviral Packaging Mix, Lipofectamine™ 2000 Reagent and blasticidin and is designed to be used with a...

Expressway™Lumio™ Expression and Detection System, without vector Invitrogen™

The Expressway™ Lumio™ Expression and Detection System takes advantage of the Lumio™ recognition sequence, a small, six amino acid sequence (Cys-Cys-Pro-Gly-Cys-Cys). The Lumio™ detection reagent binds the recognition sequence with high specificity and affinity, resulting in a bright fluorescent...

Ni-NTA Purification System Invitrogen™

The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different...

T-REx™ Complete Kit, with pcDNA™4/TO© Vector Invitrogen™

A Tetracycline-Regulated Expression System without Viral Transactivators The T-REx™ System yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance...

β-Gal Assay Kit Invitrogen™

The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing the lacZ gene. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments.

β-Gal Staining Kit Invitrogen™

The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its...

pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli Invitrogen™

The pENTR™/SD/D-TOPO™ Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO™ Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System.

Champion™ pET102 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli Invitrogen™

The Champion™ pET Expression System yields the highest-level protein production in E. coli. During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein.

Champion™ pET100 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli Invitrogen™

The Champion™ pET Expression System yields the highest-level protein production in E. coli. During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein.

GeneSwitch™ Mammalian Expression Kit, complete kit Invitrogen™

Switch-On Expression from the Lowest Basal Levels The GeneSwitch™ System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites...

T-REx™ Jurkat Cell Line Invitrogen™

T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ.

Flp-In™-3T3 Cell Line Invitrogen™

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus.

Yeast Nitrogen Base Invitrogen™

Yeast nitrogen base (YNB) is a base medium for preparation of minimal and synthetic defined yeast media. YNB contains ammonium sulfate but does not contain amino acids. Both bulk and convenient single-use pouches are available.

Flp-In™-CHO Cell Line Invitrogen™

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus.

Flp-In™-Jurkat Cell Line Invitrogen™

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus.

pcDNA™6/TR vector Mammalian Expression Vector Invitrogen™

The pcDNA™6⁄TR vector is part of the T-REx™ System (catalog K102001), which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to...

Vivid Colors™ pcDNA™6.2/N-YFP-DEST Vector Invitrogen™

The Vivid Colors™ Fluorescent Protein Gateway™ Destination Vectors allow you to quickly and easily fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (1,2) using the pcDNA™ 6.2 Gateway™ mammalian expression vector.

Vivid Colors™ pLenti6.3/V5-GW/EmGFP Expression Control Vector Invitrogen™

The Vivid Colors™ pLenti6.3⁄V5-GW⁄EmGFP Expression Control Vector is a ViraPower™ HiPerform™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP). It is designed for use with the ViraPower™ HiPerform™ Lentiviral Expression Systems as a positive control to enable...

Spheroplast Kit for Yeast Invitrogen™

The Spheroplast Kit contains qualified reagents for the preparation of yeast spheroplasts. The reagents have been optimized for use with Pichia pastoris and are guaranteed to generate 70% spheroplasts.

pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli Invitrogen™

The pENTR™/D-TOPO™ Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO™ Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System.

pTrcHis2 TOPO™ TA Expression Kit Invitrogen™

The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO™ or pTrcHis2-TOPO™, includes a minicistron element for enhanced...

DES™-Inducible/Secreted Kit with pCoBlast Invitrogen™

The DES™-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper...

ViraPower™ Adenoviral Promoterless Gateway™ Expression Kit Invitrogen™

The ViraPower™ Adenoviral Promoterless Gateway™ Expression Kit combines Invitrogen’s ViraPower™ Adenoviral and Gateway™ technologies to facilitate easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells.

pLenti6/V5 Directional TOPO™ Cloning Kit Invitrogen™

The pLenti6⁄V5 Directional TOPO™ Cloning Kit contains the TOPO™-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells.

MagicMedia™ E. coli Expression Medium Invitrogen™

MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture O.D. (optical densities) or adding inducer. The proprietary MagicMedia™ formulation enables E.

S.c. EasyComp™ Transformation Kit Invitrogen™

The S.c. EasyComp™ Transformation Kit is designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells. Advantages over commonly used transformation procedures such as spheroplast formation or LiCl methods include: • Cells can be made competent in less than 30 minutes •...

Expressway™ Maxi Cell-Free E. coli Expression System Invitrogen™

The Expressway™ Maxi Cell-Free E. coli Expression System uses an efficient, coupled transcription/translation reaction to produce milligram quantities of soluble, functionally active protein in 4-6 hours.

pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli Invitrogen™

The pENTR™/D-TOPO™ Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO™ Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System.

pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli Invitrogen™

The pENTR™/TEV/D-TOPO™ Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO™ Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System.

ViraPower™ Zeo Lentiviral Support Kit Invitrogen™

The ViraPower™ Zeo Lentiviral Support Kit contains the necessary components to transfect 293FT cells to generate viral particles containing the target gene. The kit contains the ViraPower™ Lentiviral Packaging Mix, Lipofectamine™ 2000 Reagent and Zeocin™ and is designed to be used with a ViraPower™...

PichiaPink™ Vector Kit Invitrogen™

The PichiaPink™ Vector Kit contains the pPINK-HC and pPINK-LC vectors for use with the PichiaPink™ Yeast Expression System. The pPINK vectors are built around the ADE2 gene for complementing the ade2 deletion in the PichiaPink™ strains.

BacMam pCMV-Dest Vector Invitrogen™

The BacMam pCMV-Dest Vector combines Gateway™ cloning and BacMam gene expression technologies for easy recombination-based cloning and baculovirus-based expression of a target gene in variety of cell types.

pJTI™ R4 EXP CMV-TO EmGFP pA Vector

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection,...

pJTI™ R4 CMV-TO MCS pA Vector

The pJTI™ R4 CMV-TO MCS pA vector is designed for the expression of your gene of interest under the control of the tet-inducible CMV promoter after restriction enzyme cloning and retargeting into the genomic R4 site of a Jump-In™ parental cell line.

Gateway™ pENTR™ 11 Dual Selection Vector Invitrogen™

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit Invitrogen™

The ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit contains the MultiSite Gateway™-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4⁄R4R2⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any...

pJTI™ R4 Dest CMV N-EmGFP pA Vector Thermo Scientific™

The pJTI™ R4 Dest CMV N-EmGFP pA vector allows a gene of interest to be expressed as an N-terminal Emerald Green Fluorescent Protein (EmGFP) fusion in a Jump-In™ parental cell line. When used in combination with a Jump-In™ parental cell line kit such as the Jump-In™ GripTite™ HEK293 cell line...

X-33, Pichia pastoris Yeast Strain Invitrogen™

Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain.

EKMax™ Enterokinase Invitrogen™

EKMax™ is a recombinant preparation of the catalytic subunit of bovine enterokinase (1). EKMax™ recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this peptide sequence (Figure 1).

ViraPower™ BacMam Expression System Invitrogen™

The ViraPower™ BacMam Expression System provides BacMam pCMV-Dest Vector, Cellfectin™ II Reagent, and MAX Efficiency™ DH10Bac™ Competent Cells for easy recombination-based cloning and baculovirus-based expression of a target gene.

Gateway™ pDEST™26 Vector Invitrogen™

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins.

Gateway™ pDEST™15 Vector Invitrogen™

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins.

Gateway™ pDEST™24 Vector Invitrogen™

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins.

pcDNA™3.1/nV5-DEST Mammalian Expression Vector Invitrogen™

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins.

Bac-to-Bac™ Vector Kit Gibco™

The Bac-to-Bac™ Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac™ Baculovirus Expression System (Cat. No. 10359-016), which enables the efficent production of recombinant baculovirus for expression testing in insect cells.

MaV203 Competent Yeast Cells, Library Scale Invitrogen™

MaV203 Competent Yeast Cells are designed for use with the ProQuest™ Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems.

Plasmid pCMV•SPORT-βgal Invitrogen™

Plasmid pCMV·SPORT-bgal is used as a positive control for monitoring expression in eukaryotic cells. The plasmid contains the reporter gene b-galactosidase (b-gal) from E. coli cloned as a Not I fragment into plasmid pCMV·SPORT1.

pcDNA™3.2-DEST Mammalian Expression Vector Invitrogen™

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression •attR sites for Gateway™ cloning, enabling recombination with...
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