DNA Vectors

DNA Vectors

DNA vectors are molecules used as a vehicle to transfer foreign genetic material into a host cell where it can be replicated and/or expressed. These products include kits for cloning, transfection, and expression of DNA.
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Champion™ pET100 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli Invitrogen™

The Champion™ pET Expression System yields the highest-level protein production in E. coli. During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein.

pTrcHis2 TOPO™ TA Expression Kit Invitrogen™

The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO™ or pTrcHis2-TOPO™, includes a minicistron element for enhanced...

ViraPower™ Lentiviral Directional TOPO™ Expression Kit Invitrogen™

The ViraPower™ Lentiviral Directional TOPO™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO™ technologies to facilitate quick PCR-based cloning...

ViraPower™ Bsd Lentiviral Support Kit Invitrogen™

The ViraPower™ Bsd Lentiviral Support Kit contains the necessary components to transfect 293FT cells to generate viral particles containing the target gene. The kit contains the ViraPower™ Lentiviral Packaging Mix, Lipofectamine™ 2000 Reagent and blasticidin and is designed to be used with a...

Expressway™ Maxi Cell-Free E. coli Expression System Invitrogen™

The Expressway™ Maxi Cell-Free E. coli Expression System uses an efficient, coupled transcription/translation reaction to produce milligram quantities of soluble, functionally active protein in 4-6 hours.

Ni-NTA Purification System Invitrogen™

The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different...

ViraPower™ BacMam Expression System Invitrogen™

The ViraPower™ BacMam Expression System provides BacMam pCMV-Dest Vector, Cellfectin™ II Reagent, and MAX Efficiency™ DH10Bac™ Competent Cells for easy recombination-based cloning and baculovirus-based expression of a target gene.

pJTI™ R4 Dest CMV N-EmGFP pA Vector Thermo Scientific™

The pJTI™ R4 Dest CMV N-EmGFP pA vector allows a gene of interest to be expressed as an N-terminal Emerald Green Fluorescent Protein (EmGFP) fusion in a Jump-In™ parental cell line. When used in combination with a Jump-In™ parental cell line kit such as the Jump-In™ GripTite™ HEK293 cell line...

BacMam pCMV-Dest Vector Invitrogen™

The BacMam pCMV-Dest Vector combines Gateway™ cloning and BacMam gene expression technologies for easy recombination-based cloning and baculovirus-based expression of a target gene in variety of cell types.

pJTI™ R4 EXP CMV-TO EmGFP pA Vector

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection,...

pBAD202 Directional TOPO™ Expression Kit Invitrogen™

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence.

pTrcHis TOPO™ TA Expression Kit Invitrogen™

The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO™ or pTrcHis2-TOPO™, includes a minicistron element for enhanced...

NT-GFP Fusion TOPO™ Expression Kit Invitrogen™

The GFP Fusion TOPO™ TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle 3 GFP protein. These kits provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO™ or pcDNA3.1/CT-GFP-TOPO™ vectors (Figure 1).

pcDNA™3.1/V5-His TOPO™ TA Expression Kit Invitrogen™

The pcDNA™3.1/V5-His TOPO™ TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO™ vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants.

Gateway™ pENTR™ 1A Dual Selection Vector Invitrogen™

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!
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