Shop All Nucleic Acid Labeling and Detection

Quant-iT™ PicoGreen™ dsDNA Assay Kit Invitrogen™

Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products.

PicoGreen® dsDNA Quantitation Reagents are:

• Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1)
• Specific for dsDNA in the presence of equimolar amounts of RNA (Figure 2)
• Easy to use-just add the dye to sample, wait 5 minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers and Fluorescence

Applications: The PicoGreen® dsDNA Quantitation Reagent and Kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.

ReadyProbes™ In Situ Hybridization (ISH) Blocking Solution (5X) Invitrogen™

ReadyProbes In Situ Hybridization (ISH) Blocking Solution minimizes non-specific background staining in chromogenic in situ hybridization (CISH) or fluorescence in situ hybridization (FISH) procedures. It is provided as a 5X solution that is diluted to 1X with distilled or deionized water. After the labeled DNA or RNA probe hybridization and wash steps, the sample is incubated with 1X Ready Probes ISH Blocking Solution for 30–60 minutes. 1X ReadyProbes ISH Blocking Solution can also be used as diluent for antibodies, avidin, streptavidin, or biotin during incubation steps.

Pierce™ Biotin 3' End DNA Labeling Kit Thermo Scientific™

The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency.

Features of the Biotin 3' End DNA Labeling Kit:

• Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
• 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
• Biotin-labeled probes are stable for more than one year
• 30-minute labeling procedure is fast and efficient

The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (Biotin-11-UTP) onto the 3' end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.

PrimeFlow™ Fixation/Permeabilization Buffer Set Invitrogen™

The PrimeFlow™ Fixation/Permeabilization Buffer Set is a component of the PrimeFlow™ RNA Assay kit (cat. 88-18001-204 and 88-18009-204). These buffers can be used to evaluate intracellular staining antibody performance following the fixation and permeabilization steps of the assay. Note that additional changes to antibody performance may be observed after the hybridization steps of the PrimeFlow™ RNA assay.

Please refer to the PrimeFlow™ RNA Assay protocol on the product data sheet for instructions for use.

QuantiGene™ Plex 384-well Assay Kit Invitrogen™

The QuantiGene Plex 384-well Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 384-well plates and must be used with a target-specific QuantiGene Plex panel (sold separately). See related sample preparation reagents and RNA controls ›

The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets. The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals, which are read with a Luminex xMAP instrument (FlexMap3D).

Features of the QuantiGene Plex 384-well Assay Kit include:
Accomodates difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and blood
True multiplexing—measures up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity
Standardized platform—uses 384-well plate format compatible with Luminex FlexMap3D systems
Simple workflow—employs an ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling
Large inventory of validated genes—select from over 22,000 genes to create pathway and disease-themed panels
Rapid turnaround for custom panels—a custom panel can be created within three weeks

Technology
QuantiGene assays utilize branched DNA (bDNA) technology that has been used for decades in various applications and areas of biology. First, cells are lysed or tissue samples are homogenized to release the target RNA. Second, the oligonucleotide probe set is incubated with the target RNA and Luminex xMAP bead set overnight. During this incubation, the probes cooperatively hybridize to the target and capture probes bound to each bead, capturing the target RNA. Third, signal amplification is performed via sequential hybridization of the bDNA pre-amplifier, amplifier, and label probe molecules to the target. Addition of streptavidin phycoerythrin generates a luminescent signal directly proportional to the amount of target mRNA present in the sample.

Applications
QuantiGene Plex 384-well Assay applications include compound screening of drug candidates (primary screening), biomarker verification (secondary screening), siRNA knockdown efficiency, prospective and/or retrospective analysis of samples, microarray validation, predictive toxicology, and detection of translocations and fusion genes.

ViewRNA™ Tissue Assay Core Kit Invitrogen™

The ViewRNA Tissue Assay Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).

ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.

With ViewRNA Tissue assays, you can achieve:
• Robust, single mRNA molecule detection in tissue sections
• Accuracy and specificity inherent to branched DNA amplification technologies
• Simultaneous detection of two RNA targets

The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.
 

Qubit™ RNA HS Assay Kit Invitrogen™

The Qubit RNA HS Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein, are well-tolerated in the assay. The assay kit is designed to be accurate for RNA sample concentrations between 250 pg/µL and 100 ng/µL. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer.

Which product to choose for RNA HS (High Sensitivity) quantitation?
• For 1–20 samples: use this Qubit RNA HS Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT RNA HS Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

PrimeFlow™ RNA Assay Kit Invitrogen™

This PrimeFlow™ RNA Assay is an in situ hybridization assay that combines the power of branched-DNA technology with the single-cell resolution of flow cytometry, enabling the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell surface and intracellular proteins using fluorochrome-conjugated antibodies. Branched-DNA technology uses pairs of target-specific probes to amplify the detection of a RNA transcript, achieving between 8,000- to 16,000-fold signal amplification from a desired RNA transcript. PrimeFlow RNA Assay can detect mRNA, long non-coding RNA, viral RNA, and telomere DNA. The detection of microRNA is also possible and is optimal when using the PrimeFlow™ microRNA Pretreatment Buffer (cat. 88-18006) and protocol. PrimeFlow RNA Assay is compatible with human and mouse cells. Other mammalian and non-mammalian cells may be compatible

This PrimeFlow RNA Assay contains all the reagents needed to conduct the assay. Positive control probes and target probes for genes of interest are sold separately.

Reported Application
RNA Flow Cytometry

Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit Invitrogen™

The Click-iT® RNA Alexa Fluor® 488 Imaging Kit enables detection of global RNA transcription temporally and spatially in cells and tissues (PNAS (2008) 105:15779-84).
The ability to detect newly synthesized RNA or changes in RNA levels resulting from disease, environmental damage or drug treatments is an important aspect of toxicological profiling. Utilizing an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and powerful click chemistry, newly synthesized RNA can be detected without the use of radioactivity or antibodies with a simple, two-step procedure. In step one, the alkyne-containing nucleoside is fed to cells or animals and actively incorporated into nascent RNA. The small size of the tag enables efficient incorporation of the modified nucleoside into RNA, but not into DNA. Detection utilizes the chemoselective ligation or “click" reaction between and azide and an alkyne where the modified RNA is detected with a corresponding azide-containing dye. With its dimunitive “footprint", the Click-iT® detection molecule can easily penetrate complex samples and leaves open the possibility of multiplex analyses with other probes, including antibodies for the detection of RNA-interactive proteins for deeper biological insights.

QuantiGene™ Plex Wash Buffer Components Invitrogen™

QuantiGene Plex Wash Buffer Components are aqueous buffered solutions that are mixed with nuclease-free water to make QuantiGene Plex wash buffer. The wash buffer components may be purchased in 1, 3, or 10-plate quantities to supplement QuantiGene Plex Assay kits when additional wash buffer volume is required for automation.

GeneChip™ WT Terminal Labeling and Controls Kit Applied Biosystems™

The GeneChip™ WT Terminal Labeling and Controls Kit should be combined with the WT Expression Kit (sold separately) as the most effective option for target preparation on GeneChip Exon and Gene 1.0 ST Arrays. The basic chemistry is the same as the GeneChip WT Sense Target Labeling and Control Reagents kit; however, the WT Expression Kit utilizes a streamlined protocol with master mixes, does not require an upfront rRNA-reduction step for optimal performance, and uses optimized reagents that enable lower total RNA input amounts.

The GeneChip WT Terminal Labeling and Controls Kit contains the GeneChip WT Terminal Labeling Kit, GeneChip Poly-A Control Kit, and GeneChip Hybridization Control Kit.

Key Features

WT Expression Kit:
   - 50 ng total RNA input
   - No ribosomal removal required for Human, Mouse, and Rat Exon 1.0 ST and Gene 1.0 ST Arrays
   - Streamlined workflow for a simplified protocol and reduced hands-on time
   - Magnetic bead-based purification for enhanced recovery

The WT Expression Kit includes:
   - All reagents needed to generate sense cDNA ready for fragmentation and labeling
   - Magnetic beads for enhanced purification efficiency
   - Plastic consumables required to run the assay

The GeneChip WT Terminal Labeling and Controls Kit includes:
   - WT Fragmentation and Labeling Kit required for target produced by the WT Expression Kit
   - Poly-A controls that are added to input RNA to measure efficiency of target amplification by the WT Expression Kit
   - Hybridization controls that monitor the hybridization process for troubleshooting purposes

What you need to order
• For 10 reactions:
   - WT Expression Kit (Cat. No. 4411973), 10 reactions
   - GeneChip WT Terminal Labeling and Controls Kit (Cat. No. 901525), 10 reactions*
   *Additional GeneChip WT Terminal Labeling Kit, 10 reactions (Cat. No. 900670), is available separately.

• For 30 reactions:
   - WT Expression Kit (Cat. No. 4411974), 10 reactions
   - GeneChip WT Terminal Labeling and Controls Kit (Cat. No. 901524), 10 reactions*

Documentation
The following documentation is required for using the WT Expression Kit and the GeneChip WT Terminal Labeling and Controls Kit with Exon and Gene 1.0 ST Arrays.
Protocol, WT Expression Kit (Cat. No. 4425209)
User Manual, GeneChip WT Terminal Labeling and Hybridization (Cat. No. 702808)
User Manual, GeneChip Expression Wash, Stain and Scan (Cat. No. 702731)

Related Links
GeneChip™ Hybridization, Wash, and Stain Kit
GeneChip™ WT Terminal Labeling and Controls Kit
atSNPtilx520433 Arabidopsis Cartridge Array

xMAP™ Sheath Fluid Invitrogen™

xMAP Sheath Fluid is the delivery medium that carries the sample to the optics component of Luminex xMAP technology-based instruments. It is needed to operate all Luminex 200 and FLEXMAP 3D systems. It is also available at a 20X concentration that is diluted before use (Cat. No. 4050018).

QuantiGene™ Plex Human Virus-Host Panel Invitrogen

The QuantiGene Plex Human Virus-Host Panel is a 50-plex panel focusing on RNA targets relevant to respiratory virus infection biology in humans and includes targets related to immune response, viral infection process, cytokine storm, viral RNAs (SARS-CoV-2, influenza, respiratory syncytial virus/RSV), and more. This panel was functionally verified. If a particular target of interest is not included, a custom panel can be designed. Every new and custom panel is designed, built, validated, and shipped within three weeks of your order. This panel is used in combination with the QuantiGene Plex Assay Kit, which contains all buffers, plates, and detection reagents necessary to perform the assay in 96- or 384-well plates.

Features of the QuantiGene Plex Human Virus-Host Panel include:
True multiplexing—measure 50 genes of interest and housekeeping genes in the same well with no cross-reactivity
Accommodation of difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and blood
Standardized platform—uses 96 or 384-well plate formats compatible with Luminex 200, MagPix, and FlexMap3D systems
Simple workflow—employs an ELISA-like workflow with direct hybridization of transcripts to beads and transcript labeling
Large inventory of validated targets— if additional targets need to be added to this panel, select from over 22,000 genes to create pathway and disease-themed panels
Rapid turnaround for custom panels—if we don’t have your gene(s), we can create a custom panel within three weeks

QuantiGene Plex assays are hybridization-based assays for the direct quantitation of RNA directly from cell lysates, tissue homogenates, or purified RNA, and they enable the measurement of multiple targets in the same well via multiplexing. QuantiGene Plex assays contain two components: a target-specific probe set and magnetic capture beads. They use the target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals which are read with a Luminex xMAP instrument.

Targets: SARS-CoV-2 pan probe (orf1ab, Membrane Glycoprotein, Nucleocapsid), ORF3a, ORF7a, ORF8, RdRP/NSP12, S protein, TMPRSS2, ACE2, AT2, NF-κB, DPP4 (CD26), Envelope Protein, Influenza Pan Probe, RSV Pan Probe, CD4, CD8a, HLA-DR, CD38, CD25, CD14, CD19, RORc, TBX21, GATA3, NCAM1 (CD56), CD11d, IFITM1, CCL2 (MCP-1), CCL3 (MIP-1a), IFNg, Il12A, IL6, IL8a, IL1b, IFNa, Il10, IL2, TNFa, IL4, Granzyme B, IL18, IP10 (CXCL10), GM-CSF, IL17A (CTLA8), VEGF-A, GAPDH, GUSB, HPRT1, PPIB, RPL13A

Technology
QuantiGene Plex assays utilize branched DNA (bDNA) technology. First, cells (or blood) are lysed or tissue samples (including FFPE) are homogenized to release the target RNA. Second, the oligonucleotide probe set is incubated with the target RNA and Luminex xMAP bead set overnight. During this incubation, the probes cooperatively hybridize to the target and capture probes bound to each bead, capturing the target RNA. Third, signal amplification is performed via sequential hybridization of the bDNA preamplifier, amplifier, and label probe molecules to the target. Addition of streptavidin phycoerythrin generates a luminescent signal directly proportional to the amount of target mRNA present in the sample. This allows for (relative) quantification of gene expression and with this specific panel enables study of viral infection processes, human (host) immune profile, and cytokine storm biomarkers, as well as presence of SARS-CoV-2 and select influenza and RSV strains.

Applications
QuantiGene Plex assay research applications include compound screening for drug discovery, biomarker verification, siRNA knockdown efficiency, prospective and/or retrospective analysis of clinical trial samples, microarray validation, predictive toxicology, and detection of translocations and fusion genes.

Qubit™ dsDNA BR Assay Kit Invitrogen™

The Qubit dsDNA BR Assay Kit is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentration from 100 pg/µl–1,000 ng/µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for dsDNA BR (Broad Range) quantitation?
• For 1–20 samples: use this Qubit dsDNA BR Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT dsDNA BR Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Microplates for Fluorescence-based Assays, 96-well Invitrogen™

Molecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. These plates are compatible with standard fluorescence microplate readers and are offered in a convenient 10-pack size for those times when only a few assay plates are required.
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