Shop All DNA Extraction and Purification

DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples Invitrogen™

DNAzol® Reagent is a complete and ready-to-use organic reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol® Reagent procedure can be completed in typically 10–30 minutes with DNA recoveries of 70–100%. DNAzol® Reagent is:

Versatile—can use with a broad spectrum of sample materials
Efficient—rapid isolation and high recovery of genomic DNA

Efficient isolation of genomic DNA from a variety of sample types
The DNAzol® Reagent procedure uses a novel guanidine-detergent lysing solution that permits selective precipitation of DNA from cell lysate. 1 mL of DNAzol® Reagent can be used to isolate genomic DNA from 1–3 x 107 cells, from 0.1 mL of whole blood, or from 25–50 mg tissue.

Rapid isolation and high recovery of genomic DNA
The DNAzol® Reagent combines reliability, efficiency, and simplicity in its DNA isolation protocol. During isolation, a biological sample is lysed (or homogenized) in DNAzol® Reagent, and the genomic DNA is then precipitated from the lysate with ethanol. Following an ethanol wash, DNA may be solubilized in either water or 8 mM NaOH. The entire procedure can be completed in 10–30 min with DNA recovery of 70–100%.

Isolated DNA can be used for a number of downstream applications
Genomic DNA extracted using the DNAzol® procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.

UltraPure™ Salmon Sperm DNA Solution Invitrogen™

UltraPure™ Salmon Sperm DNA Solution is a ready-to-use, sheared DNA solution that is used directly in the preparation of prehybridization and hybridization solutions. This DNA solution is prepared from highly pure, phenol/chloroform-extracted DNA and DNase-free, RNase-free (DEPC-treated), distilled, deionized water. The DNA is sheared to an average size of ≤2,000 bp. The concentration is adjusted to 10 mg/mL.

Applications
Such solutions are used to block the nonspecific attachment of probe to the surface of a membrane.

Performance and quality testing
Concentration, size verification, viscosity, and ultraviolet absorbance (A260/A280) are analytically tested.

MPC™-6 (Magnetic Particle Concentrator) Thermo Scientific™

The MPC™-6 (Magnetic Particle Concentrator) holds six tubes with variable diameters (10–30 mm). Optimal working volume: 5–15 mL. Magnetic particle concentrators are designed to obtain optimal binding of Dynabeads™ with targets (e.g., cells, proteins, and nucleic acids).

GeneJET Plasmid Maxiprep Kit Thermo Scientific™

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

Efficient—high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
High concentration of DNA purified—> 400 ng/ µL
Convenient—spin column format, centrifuge or vacuum protocols
Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
In vitro transcription

Wash Buffer B for Dynabeads™ mRNA Purification Kits Invitrogen™

This Wash Buffer B is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61006, 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer B than is provided in the kit.

PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit Invitrogen™

The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 1.5 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.

Advantages of the PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit include:
Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
Low endotoxin plasmid—the resulting plasmid contains <1 EU/µg endotoxins
High yields—isolate up to 1.5 mg of high-quality plasmid DNA
Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

ChargeSwitch™ gDNA Normalized Buccal Cell Kit Invitrogen™

The ChargeSwitch® gDNA Normalized Buccal Cell Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from human buccal swabs in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Ideal for STR profiling
• Compatible with high-throughput protocols

Rapid Purification Protocol
The ChargeSwitch® gDNA Normalized Buccal Cell Kit allows purification of genomic DNA from human buccal cell swabs or pelleted cells from a mouthwash in less than 15 minutes after lysates preparation. ChargeSwitch® gDNA Normalized Buccal Cell Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. High-quality genomic DNA is purified at a normalized concentration of 1-3 ng/ µl in a total volume of 150 µl (Figure 1) by eliminating the need for lengthy quantitation and dilution steps. Samples can be stored for up to 2 weeks at 4°C before processing without a noticeable loss in DNA yield or quality.

Note: DNA yield varies and is dependent on several factors including the technique of the person taking the swab, whether the donor is a high or low shedder, and the type of swab used.

Validated for Single Tandem Repeat (STR) Profiling
After employing the ChargeSwitch® gDNA Normalized Buccal Cell Kit, purified gDNA is suitable for use in STR analysis of poor or environmentally degraded samples, and for downstream applications such as PCR.

Compatible with Single Tube and High-Throughput Protocols
The ChargeSwitch® gDNA Normalized Buccal Cell Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette.

For processing up to 24 individual samples at a time the MagnaRack™ two-piece magnetic separation tube rack is required. This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch® products.

For high-throughput applications utilizing 96 well blocks, the 96-Well Magnetic Separator is required. This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station.

For Research Use Only. Not intended for diagnostic procedures.

GeneJET Plasmid Miniprep Kit Thermo Scientific™

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.

Highlights

Efficient—high yields of up to 20 µg of high quality plasmid DNA
Fast—procedure takes less than 14 minutes
Convenient—no phenol/chloroform extraction or alcohol precipitation required
Pure—purified DNA is immediately ready to use

Applications

• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
In vitro transcription
• Transformation

Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol

Related Products
RNase A
Elution Buffer for GeneJET Plasmid Miniprep Kit
Lysis Solution for GeneJET Plasmid Miniprep Kit
Resuspension Solution for GeneJET Plasmid Miniprep Kit
Neutralization Solution for GeneJET Plasmid Miniprep Kit
Wash Solution for GeneJET Plasmid Miniprep Kit
Binding Buffer for GeneJET Plasmid Miniprep Kit

UltraPure™ Calf Thymus DNA Solution Invitrogen™

UltraPure™ DNA solutions from Calf Thymus, Herring Sperm, or Salmon Sperm are ready-to-use, sheared DNA solutions that are used directly in the preparation of prehybridization and hybridization solutions. These prepared solutions are used to block the non-specific attachment of probe to the surface of a membrane. The DNA solutions are prepared from highly pure, phenol/chloroform-extracted DNA and DNase-free and RNase-free (DEPC-treated) distilled, deionized water and are sheared to an average size of <=2,000 bp. The concentration is adjusted to 10 mg/ml.

Performance and Quality Testing: Concentration, size verification, viscosity, and ultraviolet absorbance (A260/A280) are analytically tested.

FastVac™ Vacuum Manifold Thermo Scientific™

The Thermo Scientific FastVac Vacuum Manifold features durable chemical-resistant construction and is capable of processing up to 20 samples simultaneously using a vacuum, eliminating the need for multiple centrifugation steps and disposal of flow-through from collection tubes. The one-way Luer-Lock stopcocks are compatible with most nucleic acid purification columns and individually controlled, allowing the processing of as few as one sample at a time. Furthermore, the extra-large waste reservoir makes the FastVac Vacuum Manifold convenient for protocols that require the processing of large volumes, such as during plasmid DNA isolation using a PureLink Fast Low-Endotoxin plasmid purification kit.

Product features:
Capacity—capable of processing 1 to 20 samples simultaneously using columns and a vacuum source
Composition—consists of a linear, low-density polyethylene manifold body, one-way Luer-Lock stopcocks made of a polycarbonate body and polypropylene handle, a polypropylene vacuum hose connector, and a neoprene stopper
Compatibility—compatible with most nucleic acid purification kits (refer to the kit instruction manual for information regarding compatibility and processing with a vacuum manifold)

AutoMate Express™ Forensic DNA Extraction System, with service install Applied Biosystems™

The AutoMate Express Forensic DNA Extraction System is an easy to use, robust bench-top instrument that utilizes the PrepFiler Express and PrepFiler Express BTA chemistries packaged in pre-filled, foil sealed cartridges. We have designed the PrepFiler chemistry specifically to improve the quantity and quality of DNA isolated from forensic samples, thereby increasing the potential to obtain maximum information from downstream STR analysis.

Key Product Features:
• Designed to improve the yield and overall purity of DNA isolated from both routine and challenging forensic samples.
• Provides high quality DNA, free of PCR inhibitors and suitable for downstream applications such as quantitative real-time PCR and short tandem repeat (STR) analysis
• Simple and fast separation of substrate from lysate with the unique PrepFiler LySep Column
• Quick and easy run setup with ready-to-use pre-filled cartridges
• Closed system that minimizes the risk of contamination and transposition errors
• Processes 1 to 13 samples in a single run
• Rapid implementation with pre-validated, pre-programmed instrument protocols
• Integrated solution including novel reagents, consumables and automation to provide time saving, cost effective and reliable DNA extraction
• Improves downstream genotyping (STR) success rate and profile quality
PrepFiler Express Kit Format and Components

The Automate Express System utilizes two different configurations of PrepFiler Chemistry. The PrepFiler Express Forensic DNA Extraction Kit is suitable for the majority of standard sample types encountered in forensic laboratories such as bodily fluids on different substrates including FTA paper, cotton swabs, cotton cloth, denim and many others. The PrepFiler Express BTA Forensic DNA Extraction Kit was designed specifically for challenging samples such as bones, teeth and adhesive based samples such as cigarette butts and tape lifts. Both formats come with all the reagents and plastic components including the PrepFiler LySep Columns needed to perform 52 DNA extractions on the AutoMate Express Instrument.

Streamlined Off-Line Lysis
The PrepFiler Express Kits include the innovative PrepFiler LySep Column, which dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler Lysis buffer or PrepFiler BTA Lysis buffer is added directly into the PrepFiler LySep Column along with the sample for incubation. Next, the PrepFiler LySep Column is quickly centrifuged which permits lysate flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then simply ready to be placed on the AutoMate Express instrument. This eliminates the manual lysate and substrate transfer steps, saving time and minimizing cross contamination and sample transposition events.

For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Chargeswitch™ Pro Filter Plasmid Maxi Kit Invitrogen™

The Chargeswitch® -Pro Filter Plasmid Maxi Kit includes 10 plasmid maxipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.
The ChargeSwitch®-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification" column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding" column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.



To accomplish this, the ChargeSwitch®-Pro Filter Kit provides:

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units < 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)



How it works
The ChargeSwitch® technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (<6.5) the ChargeSwitch® surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch® protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch® method effortless.

PureLink™ Viral Lysis Buffer Invitrogen™

The PureLink™ 96 Viral RNA/DNA Kit is a 96-well silica plate system that can extract both viral RNA and DNA from 200-µl cell-free fluids which uses a low-depth 96-well centrifuge bucket for ease-of- use, all without any loss in sensitivity. These kits provide flexibility in centrifuge choice. Minimum speeds for 96 well processing of 2250 x g and a rotor bucket depth of 5 cm make this kit one of the most adaptable systems for existing laboratory centrifuges

MagMAX™ DNA Multi-Sample Ultra Kit (with extra proteinase K and DNA binding beads) Applied Biosystems™

Note: This product is identical to the MagMAX™ DNA Multi-Sample Ultra Kit (A25597) except that it comes with additional proteinase K and DNA Binding Beads, which some customers find useful for their specific applications.

The MagMAX™ DNA Multi-Sample Ultra Kit is optimized to isolate genomic DNA (gDNA) from a variety of samples, such as whole blood, buccal cells, saliva, urine, blood cards, mouth rinse, and tissue. gDNA purified with the kit is ideal for qPCR applications on sensitive, scalable platforms such as the QuantStudio™ 12K Flex System with OpenArray® Block (with AccuFill™ System). The magnetic bead–based purification format allows you to easily scale from processing 12 to 500 samples a day, making it the ideal choice for pharmacogenomics (PGx) studies.

Features of the MagMAX™ DNA Multi-Sample Ultra Kit include:
• Streamlined protocols for numerous non-invasive biological samples
• Optimized to provide DNA yields suitable for OpenArray® and Ion AmpliSeq™ applications
• Packaged for high-throughput needs

One Kit for a Diverse Set of Sample Types
Why purchase multiple purification kits to isolate gDNA from different sample types? The MagMAX™ DNA Multi-Sample Ultra Kit eliminates the headache of ordering these extra kits because it has been optimized for use with a variety of biological samples. The kit is designed for high-throughput isolation of gDNA from 1 buccal swab, 50 to 350 µL of whole blood, up to 400 µL urine (without sample concentration), up to 150 µL saliva, 1-5 mL mouth rinse, blood spot cards, and mammalian tissue samples (up to 10 mg). The protocols and magnetic bead format make the kit fully amenable to automation.

*Please note that the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor with a 24 Deep-well Head is recommended for sample volumes greater than 50 µL.

Benefits of MagMAX™ Magnetic Bead–based Purification
Magnetic beads offer many benefits compared to other technologies for isolating gDNA. Beads bind gDNA more efficiently than glass fiber filters, resulting in higher and more consistent gDNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

Accessory Products
Proteinase K (A25561), DNA Binding Beads (A25562), Wash Solution 1 Concentrate (AM8504), and Wash Solution 2 Concentrate (AM8640) are components of the kit that may also be purchased separately.

HT ExoSAP-IT™ Fast High-Throughput PCR Product Cleanup Applied Biosystems™

HT ExoSAP-IT Fast High-Throughput PCR Product Cleanup, with its shortened 14 minute protocol, is a highly stable, customized formulation of the original ExoSAP-IT reagent. It is ideal for high-throughput PCR product cleanup using liquid handling platforms and multi-channel pipettes. Multiple packaging formats (vials, 8-tube strips, 96-well plates) ensure ease of use and flexibility in experimental design.

Like the original formulation, HT ExoSAP-IT Fast reagent is a mixture of Exonuclease I and Shrimp Alkaline Phosphatase for the enzymatic removal of excess primers and dNTPs following a PCR reaction. This enzymatic cleanup reagent has a decreased viscosity yet maintains the same convenience and stability that you have come to expect from the ExoSAP-IT product line. As with all products in the ExoSAP-IT family, HT ExoSAP-IT Fast reagent allows PCR cleanup with a single pipetting step. .

HT ExoSAP-IT Fast reagent is scalable for PCR cleanup from a single tube up to a 384-well plate PCR reaction.
•14 minute protocol is half the time of standard protocols
•One-tube PCR cleanup — add HT ExoSAP-IT Fast reagent directly to PCR product
•100% sample recovery — no loss of PCR products regardless of the fragment size
•Stable at 4°C for one month
•Removes excess primers and dNTPs — does not interfere with downstream applications
   -sequencing
   -SNP analysis
   -single base extension
   -fragment analysis

Adding to our family of patented ExoSAP-IT products, HT ExoSAP-IT Fast High-Throughput PCR Cleanup reagent quickly provides accurate and consistent results in high-throughput applications. HT ExoSAP-IT Fast reagent is over 50% faster than standard HT ExoSAP-IT reagent with a 14 minute total protocol time.
•Add it directly to the PCR product and incubate at 37°C for just 7 minutes.
• After PCR treatment, inactivate it by simply heating to 80°C for another 7 minutes.

This product is ideal for high volume labs that require quick sample turn around. With such a brief and simple protocol, proper sample cleanup never needs to be sacrificed due to processing or instrument time constraints. Ensure the highest quality Sanger sequencing results while keeping sample processing moving quickly and efficiently.

PCR products cleaned up with HT ExoSAP-IT Fast reagent prior to automated sequencing displayed superior sequencing results compared to the untreated equivalent (Fig. 2).

HT ExoSAP-IT Fast reagent displayed excellent stability in all formats tested including vials, 8-tube strips, and 96-well plates. The product showed no loss in function after 10 freeze thaw cycles (Fig. 3), and is stable at variable temperatures for extended periods of time compared to enzyme mix equivalents. With excellent stability and a greater than 50% faster protocol, HT ExoSAP-IT Fast reagent is ideal for high-throughput assays where robotic platforms are utilized and large volumes of samples are processed in successive testing runs.

Collectively, these results clearly demonstrate the effectiveness of HT ExoSAP-IT Fast reagent to ensure the highest quality sequences while keeping sample processing moving quickly and efficiently.
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