Shop All DNA Extraction and Purification

UltraPure™ N,N'-Methylenebisacrylamide Invitrogen™

UltraPure™ N,N´-Methylenebisacrylamide is the most frequently used cross-linking agent for polyacrylamide gels.

Performance and Quality Testing: Polymerization time is evaluated in an SDS polyacrylamide gel system.

Fixative-Free Lysing Solution, High-Yield Lyse Invitrogen™

High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.

View a selection guide for all cell lysis and preservation solutions and products related to sample fixation and permeablization, blocking reagents, and information about the use of Dynabeads® for cell isolation.

UltraPure™ 1 M Tris-HCI Buffer, pH 7.5 Invitrogen™

UltraPure™ 1 M Tris-HCl Buffer, pH 7.5, is a premixed, pH-adjusted, sterile-filtered solution. Prepared as a 1 M concentrate, this buffer can be diluted to the desired concentration and used in molecular biology or general biochemistry applications.

Performance and quality testing
No DNase, RNase, or protease activity detected.

PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit Invitrogen™

The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.

Advantages of the PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit include:
• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains <1 EU/µg endotoxins
• High yields—isolate up to 0.4 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

Poly(A)Purist™ MAG Kit Invitrogen™

Ambion® Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures. Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA.

• Highest recovery of mRNA of any available kit
• High stringency for lowering residual rRNA content
• Single round of selection sufficient for most applications

The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits (see Figure). The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography. The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability. The entire selection procedure can be completed in as little as 45 minutes (see Figure).

Accessory Products:
A magnetic stand is required for use this kit. They are available in several formats, including the Single Place Magnetic Stand (SKU #AM10026) and the 6 Tube Magnetic Stand (SKU #AM10055).

UltraPure™ 1M Tris-HCI, pH 8.0 Invitrogen™

UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications.

Performance and Quality Testing: No DNase, RNase, or protease activity detected

MagMAX™ Plant DNA Isolation Kit Thermo Scientific™

The MagMAX™ Plant DNA Isolation Kit is designed for automated high-throughput or manual purification of DNA from a wide variety of plant species. The kit uses MagMAX magnetic bead technology, eliminating the need for phenol/chloroform extraction or alcohol precipitation. The procedures are optimized for the isolation of DNA from 10–100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, age of tissue sample, and storage conditions.

The MagMAX Plant DNA Isolation Kit relies on magnetic bead technology, enabling reproducible yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of the beads makes the technology ideal for automated high-throughput nucleic acid purification, as well as for manual purification for low sample throughput. The resulting high-quality DNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications such as PCR, qPCR, or next-generation sequencing. See bar graph below for typical genomic DNA yields from various sources.

Features of the MagMAX Plant DNA Isolation Kit include:
• Versatile—suitable for most plant types and parts, including woody, polyphenol-rich, and lignified samples
• High DNA yields—compared to traditional CTAB method
• High quality—isolates high integrity DNA, free of inhibitors and RNA contamination
• Flexible—easily adjustable for high-throughput automation platforms and manual processing

Applications
Isolated DNA is ideal for use in common molecular biology procedures, including:
• qPCR
• next-generation sequencing

Benefits of MagMAX magnetic bead–based purification
The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher™ Duo Prime or KingFisher™ Flex processors.

Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids. Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process. This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps.

Optimized bead surface chemistry
The Dynabeads™ MyOne™ Carboxylic Acid utilized in the MagMAX Plant DNA Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture. These aspects make Dynabeads magnetic technology ideal for the recovery of the DNA targets found in a variety of plant samples. Once captured, the DNA is then eluted in 150 µL and ready for downstream applications.

PrepSEQ™ Rapid Spin Sample Preparation Kit with Proteinase K Applied Biosystems™

The PrepSEQ™ Rapid Spin Sample Preparation Kit with Proteinase K is recommended for difficult to lyse bacteria like Listeria monocytogenes. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.

.
For Research Use Only. Not for use in diagnostics procedures.

MagMAX™ CORE Mechanical Lysis Module Applied Biosystems™

The Applied Biosystems MagMAX Core sample preparation system is a universal system designed to meet current and future veterinary testing needs. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing. The MagMAX CORE Mechanical Lysis Module is offered as a supplement to the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of hard-to-lyse organisms like Mycobacterium avium paratuberculosis (MAP) and bovine tuberculosis in feces, milk, and environmental samples.

For a kit that contains this Mechanical Lysis Module as well as the MagMAX CORE Nucleic Acid Purification Kit, please see Cat. No. A37487.

Features include:
• Convenience—a complete solution using the same CORE chemistry
• Confidence—higher-quality nucleic acids from difficult-to-lyse samples, including both small (0.3 g) and large (2–5 g) volume feces samples
• Flexible—purchase only what you need to lyse MAP and other hard-to-lyse organisms

The MagMAX CORE kit and the MagMAX Core Mechanical Lysis Module help make your lab more efficient through greater convenience, consistent performance, and time and cost savings.

PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit Invitrogen™

The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 1.5 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.

Advantages of the PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit include:
• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains <1 EU/µg endotoxins
• High yields—isolate up to 1.5 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

JetQuick™ Blood and Cell Culture DNA Midiprep Kit Invitrogen™

The JetQuick™ Blood and Cell Culture DNA Midiprep Kit is designed for rapid and efficient purification of genomic DNA (gDNA) from blood, body fluids (amniotic fluid, saliva, sperm, lymph), and mammalian cells. It utilizes silica membrane-based technology that allows for isolation of 20-50 kb DNA that is suitable for a wide range of downstream applications, including PCR, restriction enzyme digestion, and Southern blotting.

Key benefits include:
• Large volume processing, up to 3 mL of blood or saliva
• Long double-stranded DNA (20-50 kb) recovery
• Compatible with centrifugation or a vacuum manifold

The midiprep format enables the processing of up to 3 mL of whole blood or other body fluid. DNA from up to 1 x 10⁸ mammalian cells can be isolated. The purified DNA is ready for downstream analysis.

UltraPure™ 0.5M EDTA, pH 8.0 Invitrogen™

UltraPure™ 0.5 M EDTA, pH 8.0 is an aqueous solution prepared by dissolving Na₂EDTA•2H₂O in distilled, deionized water and adjusting the pH to 8.0 with sodium hydroxide. This sterile-filtered solution is suitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions.

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K Applied Biosystems™

The PrepSEQ® Spin Sample Preparation Kit – Extra Clean with Proteinase K is recommended for difficult to lyse bacteria, like Listeria monocytogenes, that also has a high lipid content, such as soft cheeses. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit – Extra Clean with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.

.
For Research Use Only. Not for use in diagnostics procedures.

PureLink™ HiPure Precipitator Module Invitrogen™

The PureLink™ HiPure Precipitator allows fast, simple, and efficient desalting and concentration of plasmid DNA isolated using anion-exchange chromatography. The traditional method for DNA precipitation of anion exchange isolated plasmid DNA involves isopropanol precipitation followed by centrifugation and drying of DNA,which is time consuming and labor intensive. The use of the PureLink™ HiPure Precipitator allows plasmid DNA concentration within 5 minutes, eliminates the need for centrifugation, and reduces the risk of losing the DNA pellet during supernatant removal. The recovery is >80% and resulting plasmid DNA is ready to use in the most challenging applications such as transfection.

MagMAX™ CORE Lysis Solution Applied Biosystems™

This MagMAX CORE Lysis Solution is made available as a supplement to that included in the MagMAX CORE Nucleic Acid Purification Kit. This proprietary buffer solution is used to break open cells in preparation for downstream molecular detection applications such as real time PCR, RT-PCR, and sequencing applications.