Shop All DNA Extraction and Purification

MagJET Plasmid DNA Kit Thermo Scientific™

Thermo Scientific MagJET Plasmid DNA Kit, sufficient for 4 x 96 preps, is designed for fast and efficient purification of plasmid DNA (up to 4-5 µg/mL) or high copy plasmid DNA from overnight E. coli culture.

the kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET Magnetic Beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput users.

The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors. Purified plasmid DNA can be used in a wide range of downstream applications such as PCR, qPCR and other enzymatic reactions.

Highlights

High yields – up to 5 µg of plasmid DNA from 1 mL of bacterial culture
Pure – isolated DNA is immediately ready-to-use in downstream application such as sequencing, transcription, digestion with restriction enzymes or transfection of robust cell lines
Flexible – automation and manual processing protocols available
Repeatable results and robust performance

Applications: Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
In vitro transcription
• Transformation
• Transfection of robust cell lines

PCR Product Pre-Sequencing Kit Applied Biosystems™

The PCR Product Pre-Sequencing Kit uses a novel, enzymatic clean-up method to pre-treat PCR products prior to sequencing, without any subsequent purification or separation steps. The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers present in the final PCR product reaction mixture. The enzymes are conveniently added to an aliquot of the PCR product mixture, incubated at 37°C, and then inactivated at 80°C. The result is a cleaner PCR product, free from excess nucleotides and primers, and ready to be sequenced with standard sequencing reagents.

Novex™ TBE Running Buffer (5X) Invitrogen™

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock™ Mini-Cell.

Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended Buffers: For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.

USB™ CycleSeq™ Thermostable DNA Polymerase Applied Biosystems™

USB™ CycleSeq™Thermostable DNA Polymerase is a thermostable, DNA polymerase that is exonuclease-free and incorporates ddNTPs with a marked level of efficiency over standard thermostable DNA polymerases. This allows for more even and easy-to-read sequence band patterns, making sequence anomalies easier to identify.

USB CycleSeq Thermostable DNA Polymerase can be substituted for ThermoSequenase DNA polymerase as both enzymes incorporate modified nucleotides (see Fig. 1).

CycleSeq DNA Polymerase, like Thermo Sequenase DNA Polymerase, includes Thermoplasma acidophilum inorganic pyrophosphatase (TAP). TAP is thermostable and converts pyrophosphate to orthophosphate, a useful feature during cycle sequencing reactions where pyrophosphates are produced.

USB CycleSeq Thermostable DNA Polymerase is useful for cycle sequencing (Sanger sequencing), as it produces sequence data with uniform band intensities which allows for longer and more accurate sequence reads. It is also useful for primer extension protocols during SNP genotyping.

Source:
Recombinant

Purity:
Greater than 95% pure as determined by SDS-PAGE.

Storage Buffer:
20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.053 unit/µl Thermoplasma acidophilum inorganic pyrophosphatase, 50% glycerol, and stabilizers.

Assay Conditions:
The reaction mixture contains 25 mM TAPS, pH 9.3, 50 mM KCl, 2 mM MgCl2, 1 mM Β-ME, 200 µM dATP, 200 µM dGTP, 200 µM dTTP, 100 µM dCTP, 0.05 µL [α33P] dCTP (10 µCi/µL), 400 µg/mL activated DNA, and CycleSeq Thermostable DNA Polymerase. After incubation at 74deg;C for 10 minutes, acid-insoluble material is determined (50 µL reaction volume).

Unit Definition:
One unit of enzyme is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into acid-insoluble form in 30 minutes at 74°C under standard assay conditions.

Concentration:
32 units/µL

Functional test:
Functionally tested to meet or exceed the specifications for use in dye primer sequencing. Release specifications are based on sequence length, band intensity, and sequence quality. The sequence must also be free of background bands strong enough to interfere with sequence interpretation.

PureLink™ 96 HQ Plasmid DNA Purification Kit Invitrogen™

The PureLink HQ 96 Plasmid DNA Purification Kit isdesigned for high-throughput isolation of high-qualityplasmid DNA from bacterial cells. It can beused with a vacuum manifold or a centrifuge and iscompatible with automated liquid handling workstations.

The PureLink HQ 96 Plasmid DNA Purification Kit offersthe following advantages:
• Ability to isolate up to 10 µg plasmid DNA per well
• Designed to isolate high-quality plasmid DNA in30-45 minutes
• Minimal genomic DNA contamination in the purifiedplasmid DNA
• Compatible with automated liquid handlingworkstations
• Reliable performance of the purified plasmid DNA indownstream applications such as mammaliantransfection

Process overview
Bacterial cells are harvested and resuspended in ResuspensionBuffer with RNase. The cells are lysed using an alkaline/SDScell lysis procedure. The lysate is then neutralized andconditions are adjusted for subsequent binding. After lysateclarification using the PureLink HQ 96 Clarification Plate, thelysate is processed through the PureLink HQ 96 BindingPlate. The DNA binds to the silica-based membrane in theplate and impurities are removed by washing with WashBuffer. The DNA is then eluted in low salt Elution Buffer orwater.

Chargeswitch™ Pro ET Removal Wash Buffer Invitrogen™

Chargeswitch® Pro ET Removal Wash Buffer is a component in the Chargeswitch® Pro Filter Plasmid kits. With the endotoxin removal buffer, the endotoxin levels of <10 EU⁄ug can be achievable and it can be used in all Chargeswitch® Pro plasmids.

Lysis Buffer for MagJET Plasmid DNA Kit Thermo Scientific™

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET Plasmid DNA Kits (K2791/2792) and may be purchased separately.

MagMAX™ Viral/Pathogen Binding Solution Applied Biosystems™

This binding buffer is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more binding buffer than is provided in the kit.

PureLink™ Quick Gel Extraction Kit Invitrogen™

The PureLink® Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ~30 minutes using PureLink® silica membrane-based quick gel extraction columns. For your convenience, purification protocols are provided for centrifugation and for vacuum.

Advantages of using PureLink® Quick Gel Extraction Kit:

• Purify DNA fragments from TAE and TBE agarose gels of various percentages and melting points
• Complete the procedure in ~30 minutes
• Easily purify DNA fragments from 40 bp to 10 kb from gels
• Obtain high recovery of DNA fragments
• Bind and purify up to 15 µg DNA with one column
• Purify DNA fragments that are high quality and show reliable performance in PCR, restriction enzyme digestion, cloning, and labeling

Note: The PureLink® Quick Gel Extraction Kit is not designed to purify supercoiled plasmid DNA or genomic DNA from agarose gels. Only linear DNA fragments may be purified from gels using this kit.

PureLink™ HQ Mini Plasmid DNA Purification Kit Invitrogen™

The PureLink HQ Mini Plasmid Purification Kit is designedfor the isolation of high-quality plasmid DNA that is suitablefor restriction enzyme digestion, PCR, sequencing, bacterialcell transformation, and mammalian cell transfection.Using the kit, plasmid DNA can be isolated from varyingamounts of bacterial cells.

The PureLink HQ Mini Plasmid Purification Kit has thefollowing advantages:
• Higher spin column capacity for plasmid DNA ascompared to other commercially available plasmidpurification systems
• Designed to isolate high-quality plasmid DNA in less thanan hour
• Minimal genomic DNA contamination of the purifiedsample
• Reliable performance of the purified DNA in downstreamapplications

Process overview
Bacterial cells are pelleted, resuspended, and lysed. Thelysate is then neutralized and conditions are adjusted forsubsequent binding. After clarification by centrifugation, thelysate is processed through the PureLink spin column. TheDNA binds to the silica-based membrane in the column, andimpurities are removed by a single wash step. The DNA isthen eluted in Elution Buffer or water.

Novex™ Hi-Density TBE Sample Buffer (5X) Invitrogen™

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock™ Mini-Cell.

Formulation:

Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended Buffers:

For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.

PureLink™ Pro Quick96 Plasmid Purification Kit Invitrogen™

The PureLink® Pro Quick96 Plasmid Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of plasmid DNA from E. coli in as little as 45 minutes (Table 1). Using the PureLink® Pro Quick96 Plasmid Kit, you will:

• Obtain consistently high yields of high-purity plasmid DNA
• Experience ease of use and better performance due to improved plate design and protocol
• Increase processing flexibility with protocols for centrifuge, vacuum manifold, or automated platforms

How it works
The PureLink® Pro Quick96 Plasmid Kit uses two 96-well plates, a filtration plate (clear O-ring), and a binding plate (red O-ring) to purify plasmid DNA. Bacterial cells are subjected to alkaline lysis and are pelleted by centrifugation. The bacterial lysate is then applied to the Quick96 Filtration Plate for clearing and collection in the Quick96 Binding Plate, where the plasmid DNA binds to the silica membrane. After washing steps to remove contaminants, purified DNA is eluted and ready for use in downstream applications. The entire procedure can be completed in 45 minutes (Figure 1).

Improved plate design
The PureLink® Pro Quick96 Plasmid Kit provides optimized semiskirted 96-well plates for improved performance and results (Figure 2). The Quick96 Filtration Plate is designed for fast and efficient clearing of the bacterial lysates. The Quick96 Binding Plate offers an increased binding capacity of up to 20 µg plasmid DNA. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation, or with an automated liquid handling platform. The semiskirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
The PureLink® Pro Quick96 Plasmid Kit allows rapid and reproducible purification of up to 20 µg of plasmid DNA from E. coli strains grown in up to 5 ml LB, 3 ml 2xYT, or 3 ml TB. In an automated platform using 1.5 ml E. coli culture grown overnight in a square-well block, typical yields are 5-15 µg plasmid DNA, with an average of 9.4 µg (Figure 3). The resulting plasmid DNA is supercoiled with no detectable genomic DNA or RNA (Figure 4). The PureLink® Pro Quick96 Plasmid Kit provides higher yields of high-quality plasmid DNA for use in common downstream applications such as automated sequencing, PCR, restriction enzyme digestion, and cloning.

PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit Applied Biosystems™

The Applied Biosystems® PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit rapidly extracts Mycoplasma genomic DNA from 100µl sample volumes of research cell lines, stem cell lines, and cell and tissue therapy samples. In the PrepSEQ™ 1-2-3 Kit protocol, a rapid procedure recovers DNA from total lysate of the test sample using magnetic-bead based technology. The Myco Scan Kit is optimized for use with the PrepSEQ 1-2-3 Kit.

- High recovery of Mycoplasma genomic DNA from mammalian cell culture samples
- Rapid easy to use protocol results in under 2 hours
- Compatible with the Myco Scan Mycoplasma Detection assay
- Protocol available for automated sample preparation

High DNA Recovery Sample Preparation
Optimized for use with the Myco Scan Kit, the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit uses proprietary magnetic bead-based separation technology to extract Mycoplasma DNA from mammalian cell lines. The PrepSEQ 1-2-3 Kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, cellular and tissue therapy samples with high efficiency.

The protocol is recommended for use with samples with a starting volume of 100 µl with a maximum cell density of 106 cells. The PrepSEQ 1-2-3 Kit provides an easy to use procedure consisting of 1) RNase treatment 2) Proteinase treatment and 3) DNA extraction using the magnetic bead technology (Figure 1). The kit isolates Mycoplasma genomic DNA with high sensitivity from total lysate of test sample. The PrepSEQ 1-2-3 Kit and the Myco Scan Kit allow routine screening of high-value cell lines using an easy, rapid and sensitive protocol. Figure 2 demonstrates how a sample that was spiked with low levels of Mycoplasma bovoculi DNA was found negative for Mycoplasma contamination using a commercially prepared kit. When using the PrepSEQ 1-2-3 Kit and the Myco Scan Kit, the correct positive call for Mycoplasma contamination was made.

QuantiGene™ Sample Processing Kit, blood samples Invitrogen™

This QuantiGene Sample Processing Kit for blood samples contains reagents and instructions for the preparation of cell lysates from whole blood samples for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA or DNA targets.

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your whole blood or dried blood spot sample

What you get
The kit contains our proprietary lysis mixture, proteinase K, and detailed instructions for preparing whole blood from heparin, citrate, or EDTA tubes, PAXgene blood RNA tubes, Tempus™ blood RNA tubes, or from dried blood spots prepared from standard anticoagulant venous blood or finger sticks.

UltraPure™ Agarose Invitrogen™

UltraPure™ Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 100 bp to >30 kb. Features of UltraPure™ Agarose:

• Ideal for analysis and recovery of DNA and RNA for routine applications
• Strong gel structure allows for better handling and less breakage
• Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay)

Improved packaging
The new environmentally friendly packaging uses 75% less plastic than the original bottles. This means less energy and raw material used in manufacture, and less waste in landfills. Additionally, the easy-pour spout reduces the likelihood of spills and contamination.

Performance and quality testing
The performance of UltraPure™ Agarose is evaluated to satisfy set specifications in appearance, moisture, gel strength, gelling temperature, melting temperature, sulfate content, electroendosmosis, and DNase/RNase activity.
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