Shop All Nucleic Acid Synthesis

iBu-rG Phosphoramidite, TheraPure™ grade, serum vial bottle Thermo Scientific™

iBu-rG CEP is a phosphoramidite containing 5' DMT and the nucleoside guanosine (G) with isobutyryl (iBu) base protection, for use in oligonucleotide synthesis. RNA phosphoramidites contain a 2'-OH protecting group, tBDMS (tert-butyl dimethylsilyl). Each base has unique protecting groups designed specifically to protect the RNA during the chemical synthesis process, and produce the highest amount of full length product. Alternate base protection options are also available to allow for faster deprotection and longer oligonucleotide synthesis. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 20 mL Amber bottle with a Serum Vial neck style, which is compatible with most ABI Systems|AKTA Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-O-[(tert-butyl)dimethylsilyl]-guanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C50H68N7O9PSi
• Base—rG
• Base Protection—iBu
• Formula weight—970.1 g/mol
• CAS number—147201-04-5
• HPLC Purity—≥99%; M-11 Impurity: ≤0.01%
• 31P NMR Purity—≥99%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%; No single PIII impurity >0.3%

iBu-rG Phosphoramidite, TheraPure™ grade, 20-400 finish bottle Thermo Scientific™

iBu-rG CEP is a phosphoramidite containing 5' DMT and the nucleoside guanosine (G) with isobutyryl (iBu) base protection, for use in oligonucleotide synthesis. RNA phosphoramidites contain a 2'-OH protecting group, tBDMS (tert-butyl dimethylsilyl). Each base has unique protecting groups designed specifically to protect the RNA during the chemical synthesis process, and produce the highest amount of full length product. Alternate base protection options are also available to allow for faster deprotection and longer oligonucleotide synthesis. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 30 mL Amber bottle with a 20-400 Finish neck style, which is compatible with most Expedite Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-O-[(tert-butyl)dimethylsilyl]-guanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C50H68N7O9PSi
• Base Protection—iBu
• Formula weight—970.1 g/mol
• CAS number—147201-04-5
• HPLC Purity—≥99%; M-11 Impurity: ≤0.01%
• 31P NMR Purity—≥99%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%; No single PIII impurity >0.3%

iBu-rG Phosphoramidite, TheraPure™ grade, 28-400 finish bottle Thermo Scientific™

iBu-rG CEP is a phosphoramidite containing 5' DMT and the nucleoside guanosine (G) with isobutyryl (iBu) base protection, for use in oligonucleotide synthesis. RNA phosphoramidites contain a 2'-OH protecting group, tBDMS (tert-butyl dimethylsilyl). Each base has unique protecting groups designed specifically to protect the RNA during the chemical synthesis process, and produce the highest amount of full length product. Alternate base protection options are also available to allow for faster deprotection and longer oligonucleotide synthesis. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 30 mL Amber bottle with a 28-400 Finish neck style, which is compatible with most MerMade Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-O-[(tert-butyl)dimethylsilyl]-guanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C50H68N7O9PSi
• Base Protection—iBu
• Formula weight—970.1 g/mol
• CAS number—147201-04-5
• HPLC Purity—≥99%; M-11 Impurity: ≤0.01%
• 31P NMR Purity—≥99%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%; No single PIII impurity >0.3%

T Phosphoramidite, TheraPure™ grade, 28-400 finish bottle Thermo Scientific™

T CEP is a phosphoramidite containing 5' DMT and the nucleoside deoxythymidine (dT) for use in oligonucleotide synthesis. DNA phosphoramidites are the building blocks for synthesis of DNA or DNA containing oligonucleotides. Each base has unique protecting groups (except T and U) designed specifically to protect the DNA during the chemical synthesis process, and produce the highest amount of full length product. Alternative protecting groups on dC (acetyl) and dG (DMF) allow for a faster deprotection of the oligonucleotide. The TheraPure trademark denotes a highest level of phosphoramidite purity, developed with the highest stringency of of high definiteion analytics and tightly controlled impurity profiles. Ideal for any therapeutic application. This product is supplied in a 250 mL Amber bottle with a 28-400 Finish neck style, which is compatible with most MerMade Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C40H49N4O8P
• Base—T
• Formula weight—744.82 g/mol
• CAS number—98796-51-1
• HPLC Purity—≥99%; M-11 Impurity ≤0.01%; Single critical impurity ≤0.3%; Presence of dC, dG, or T impurity ≤0.03%
• 31P NMR Purity—≥99%; Sum of non-primary peaks at 140-152 ppm: ≤0.3%; No peak(s) above 170 ppm; HMT impurity (~146ppm): ≤0.10%

iBu-rG Phosphoramidite, TheraPure™ grade, wide mouth bottle Thermo Scientific™

iBu-rG CEP is a phosphoramidite containing 5' DMT and the nucleoside guanosine (G) with isobutyryl (iBu) base protection, for use in oligonucleotide synthesis. RNA phosphoramidites contain a 2'-OH protecting group, tBDMS (tert-butyl dimethylsilyl). Each base has unique protecting groups designed specifically to protect the RNA during the chemical synthesis process, and produce the highest amount of full length product. Alternate base protection options are also available to allow for faster deprotection and longer oligonucleotide synthesis. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 250 mL Opaque bottle with a Wide Mouth neck style, which is compatible with most None (Bulk) of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-O-[(tert-butyl)dimethylsilyl]-guanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C50H68N7O9PSi
• Base—rG
• Base Protection—iBu
• Formula weight—970.1 g/mol
• CAS number—147201-04-5
• HPLC Purity—≥99%; M-11 Impurity: ≤0.01%
• 31P NMR Purity—≥99%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%; No single PIII impurity >0.3%

1- Methylimidazole/THF, Capping Formula B Applied Biosystems™

Contains 4 CapB filled Trap-Pak™ bags.

Cap B is a mix of NMI (N-methylimidazole) and THF (tetrahydrofuran).

For Research Use Only. Not for use in diagnostics procedures.

T Phosphoramidite, TheraPure™ grade, 20-400 finish bottle Thermo Scientific™

T CEP is a phosphoramidite containing 5' DMT and the nucleoside deoxythymidine (dT) for use in oligonucleotide synthesis. DNA phosphoramidites are the building blocks for synthesis of DNA or DNA containing oligonucleotides. Each base has unique protecting groups (except T and U) designed specifically to protect the DNA during the chemical synthesis process, and produce the highest amount of full length product. Alternative protecting groups on dC (acetyl) and dG (DMF) allow for a faster deprotection of the oligonucleotide. The TheraPure trademark denotes a highest level of phosphoramidite purity, developed with the highest stringency of of high definiteion analytics and tightly controlled impurity profiles. Ideal for any therapeutic application. This product is supplied in a 60 mL Amber bottle with a 20-400 Finish neck style, which is compatible with most Expedite Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C40H49N4O8P
• Base—T
• Formula weight—744.82 g/mol
• CAS number—98796-51-1
• HPLC Purity—≥99%; M-11 Impurity ≤0.01%; Single critical impurity ≤0.3%; Presence of dC, dG, or T impurity ≤0.03%
• 31P NMR Purity—≥99%; Sum of non-primary peaks at 140-152 ppm: ≤0.3%; No peak(s) above 170 ppm; HMT impurity (~146ppm): ≤0.10%

ChromaTide™ Fluorescein-12-dUTP Invitrogen™

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide® Labeled Nucleotides Specifications:
• Ex/Em of dye: fluorescein-12-dUTP (495/525 nm)
• Length of alkynylamino linker: 12 atoms


Methods for Incorporating ChromaTide® Nucleotides Into Probes
• Nick translation
• Random primer labeling
• End-labeling with terminal deoxynucleotidyl transferase
• Reverse transcription
• PCR amplification


See Methods for Enzymatic Incorporation of ChromaTide® dUTPs for specific guidelines for each of these methods.

Alexa Fluor® and BODIPY® Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY® and Alexa Fluor® dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY® dyes helps ensure minimal spectral overlap. The Alexa Fluor® dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide® dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide® Reagents: Molecular Probes ChromaTide® and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Ac-dC Phosphoramidite, TheraPure™ grade, serum vial bottle Thermo Scientific™

Ac-dC CEP is a phosphoramidite containing 5' DMT and the nucleoside deoxycytidine (dC) with Acetyl (Ac) base protection, for use in oligonucleotide synthesis. DNA phosphoramidites are the building blocks for synthesis of DNA or DNA containing oligonucleotides. Each base has unique protecting groups designed specifically to protect the DNA during the chemical synthesis process and produce the highest amount of full length product. Alternative protecting groups on dC (acetyl) and dG (DMF) allow for a faster deprotection of the oligonucleotide. By using Ac-dC, Bz-dA, and DMF-dG monomers, fast cleavage and deprotection can be effected by a 1:1 mixture of aqueous ammonium hydroxide and aqueous methylamine (or AMA) in 10 min. This product is supplied in a 60 mL Amber bottle with a Serum Vial neck style, which is compatible with most ABI Systems|AKTA Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-Acetyl-2'-deoxycytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
• Molecular formula—C41H50N5O8P
• Base—dC
• Base Protection—Ac
• Formula weight—771.8 g/mol
• CAS number—154110-40-4
• HPLC Purity—≥99%; M-11 Impurity ≤0.01%; No single impurity >0.5%
• 31P NMR Purity—≥99%; Sum of non-primary peaks at 140-152 ppm: ≤0.3%

GeneArt™ Custom DNA Fragment 2001-2250bp Thermo Scientific™

The GeneArt® Gene Synthesis service offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence.

2'-OMe-Bz-A Phosphoramidite, TheraPure™ grade, serum vial bottle Thermo Scientific™

2'-OMe-Bz-A CEP is a phosphoramidite containing 5' DMT and the nucleoside adenosine (A) with 2'-O-methyl protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, 2'-O-methyl (OMe) oligo modifications display nuclease resistance, fewer off-target effects, and slightly increased hybridization affinities. These phosphoramidites are commonly used for oligos designed for antisense, siRNA, aptamer, or gRNA-based research. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 20-mL amber bottle with a serum vial neck style, which is compatible with most Applied Biosystems synthesizers and AKTA synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-O-methyl-adenosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C48H54N7O8P
• Base—A
• Base Protection—Bz
• Formula weight—887.97 g/mol
• CAS number—110782-31-5
• HPLC purity—≥99%; M-11 Impurity: ≤0.01%; Single critical impurity ≤0.3%
31P NMR purity—>99%; Sum of non-primary peaks at 140-152ppm ≤0.5%

2'-MOE-5mC Phosphoramidite, TheraPure™ grade, serum vial bottle Thermo Scientific™

2'-MOE-5mC CEP is a phosphoramidite containing 5' DMT and the nucleoside 5-methyl-cytidine (5mC) with 2'-O-methoxy-ethyl (MOE) protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, 2'-O-methoxy-ethyl (MOE) modifications display nuclease resistance, fewer off-target effects, and slightly increased hybridization affinities. These phosphoramidites are commonly used for oligos designed for antisense, siRNA, aptamer, or gRNA-based research. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 20-mL amber bottle with a serum vial neck style, which is compatible with most Applied Biosystems synthesizers and AKTA synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-N4-benzoyl-cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C50H60N5O10P
• Base—5mC
• Base Protection—Bz
• Formula weight—922.0 g/mol
• CAS number—163759-94-2
• HPLC purity—≥99%; M-11 Impurity ≤0.01%
31P NMR purity—≥99%

ChromaTide™ Texas Red™-12-dUTP Invitrogen™

Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide® Labeled Nucleotides Specifications:
• Ex/Em of dye: Texas Red®-12-dUTP (595/615 nm)
• Length of alkynylamino linker: 12 atoms


Methods for Incorporating ChromaTide® Nucleotides Into Probes
• Nick translation
• Random primer labeling
• End-labeling with terminal deoxynucleotidyl transferase
• Reverse transcription
• PCR amplification


See Methods for Enzymatic Incorporation of ChromaTide® dUTPs for specific guidelines for each of these methods.

Alexa Fluor® and BODIPY® Fluorescent Dyes Make Excellent Probes
Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY® and Alexa Fluor® dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY® dyes helps ensure minimal spectral overlap. The Alexa Fluor® dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

Long Linkers Improve Performance
The ChromaTide® dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

For complete listing of our ChromaTide® Reagents: Molecular Probes ChromaTide® and aha labeled nucleotides—Table 8.5.
For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Ac-dC Phosphoramidite, TheraPure™ grade, 28-400 finish bottle Thermo Scientific™

Ac-dC CEP is a phosphoramidite containing 5' DMT and the nucleoside deoxycytidine (dC) with Acetyl (Ac) base protection, for use in oligonucleotide synthesis. DNA phosphoramidites are the building blocks for synthesis of DNA or DNA containing oligonucleotides. Each base has unique protecting groups designed specifically to protect the DNA during the chemical synthesis process and produce the highest amount of full length product. Alternative protecting groups on dC (acetyl) and dG (DMF) allow for a faster deprotection of the oligonucleotide. By using Ac-dC, Bz-dA, and DMF-dG monomers, fast cleavage and deprotection can be effected by a 1:1 mixture of aqueous ammonium hydroxide and aqueous methylamine (or AMA) in 10 min. This product is supplied in a 250 mL Amber bottle with a 28-400 Finish neck style, which is compatible with most MerMade Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-Acetyl-2'-deoxycytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
• Molecular formula—C41H50N5O8P
• Base—dC
• Base Protection—Ac
• Formula weight—771.8 g/mol
• CAS number—154110-40-4
• HPLC Purity—≥99%; M-11 Impurity ≤0.01%; No single impurity >0.5%
• 31P NMR Purity—≥99%; Sum of non-primary peaks at 140-152 ppm: ≤0.3%

2'-OMe-Bz-A Phosphoramidite, standard grade, serum vial bottle Thermo Scientific™

2'-OMe-Bz-A CEP is a phosphoramidite containing 5' DMT and the nucleoside adenosine (A) with 2'-O-methyl protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, 2'-O-methyl (OMe) oligo modifications display nuclease resistance, fewer off-target effects, and slightly increased hybridization affinities. These phosphoramidites are commonly used for oligos designed for antisense, siRNA, aptamer, or gRNA-based research. The 'standard grade' marking denotes a baseline level of purity verified with analytical methods, and proven to be suitable for most oligo synthesis requirements. This product is supplied in a 20-mL amber bottle with a serum vial neck style, which is compatible with most Applied Biosystems synthesizers and AKTA synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-O-methyl-adenosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C48H54N7O8P
• Base—A
• Base Protection—Bz
• Formula weight—887.97 g/mol
• CAS number—110782-31-5
• HPLC purity—≥98.0%; Sum of non-primary peaks 140-152ppm: ≤0.5%
31P NMR purity—≥98.0%

Results per page
    spinner