Miscellaneous Molecular Biology Reagents

NaCl (5 M), RNase-free Invitrogen™

Ambion® Molecular biology grade, 5 M NaCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

Sodium Acetate (3 M), pH 5.5, RNase-free Invitrogen™

Ambion® Molecular biology grade, 3 M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

EDTA (0.5 M), pH 8.0, RNase-free Invitrogen™

Ambion® Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

IPTG Solution, ready-to-use Thermo Scientific™

Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization.

X-Gal Solution, ready-to-use Thermo Scientific™

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.

EDTA (0.1 mM), pH 8.0, RNase-free Invitrogen™

Ambion® 0.1 mM EDTA is a commonly recommended storage solution for RNA samples. 0.1 mM EDTA is in nuclease-free, ultrapure water. It is provided in one bottle of 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet.

MgCl₂ (1 M) Invitrogen™

Molecular biology grade, Ambion™ 1 M MgCl2 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

EDTA (0.5 M), pH 8.0 Thermo Scientific™

Thermo Scientific 0.5 M EDTA, pH 8.0 is an aqueous solution of 0.22 µm membrane-filtered ethylendiaminetetraacetic acid (EDTA), sodium salt. It's ideal for biochemistry or molecular biology applications that require a chelator for divalent cations.

MgCl₂ (magnesium chloride) (25 mM) Thermo Scientific™

Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 µm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR with Taq DNA polymerase, DreamTaq DNA Polymerase, Maxima Hot Start Taq DNA Polymerase, TrueStart Hot Start Taq DNA Polymerase, Long PCR...

X-Gal Thermo Scientific™

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.

UltraPure™ BSA (50 mg/mL) Invitrogen™

UltraPure BSA is a non-acetylated bovine serum albumin (BSA) pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL. BSA has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions.

Potassium Acetate (3 M), pH 5.5, RNase-free Invitrogen™

Ambion® Molecular biology grade, 3 M Potassium Acetate, pH 5.5 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

MagMAX™ Lysis/Binding Solution Concentrate Invitrogen™

Ambion MagMAX Lysis⁄Binding Solution Concentrate is a component of the MagMAX isolation kits listed below that is made available here for separate purchase. Provided in one 100 mL bottle. This solution is the same formulation as the lysis buffer used in Cat. Nos.

EDTA, Concentrate Thermo Scientific™

This EDTA solution is specifically formulated for use in our Heat Induced Epitope Retrieval (HIER) method. Supplied as a 100 mL aliquot of 20X concentrate or 1L ready-to-use format.

Wash Buffer B for Dynabeads™ mRNA Purification Kits Invitrogen™

This Wash Buffer B is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61006, 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer B than is provided in the kit.