Miscellaneous Molecular Biology Reagents

NaCl (5 M), RNase-free Invitrogen™

Ambion® Molecular biology grade, 5 M NaCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

RNA Gel Loading Dye (2X) Thermo Scientific™

Thermo Scientific 2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide.

DTT (dithiothreitol) Thermo Scientific™

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro.

Glycerol Thermo Scientific™

Thermo Scientific Pierce Glycerol is high-purity glycerol (greater than 99% pure) for use in molecular biology methods.

SDS, 20% Solution, RNase-free Invitrogen™

Ambion® molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.

SDS, 10% Solution, RNase-free Invitrogen™

Molecular biology grade, Ambion® SDS, 10% Solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.

dNTP Mix (2.5 mM) Invitrogen™

The same high-quality deoxynucleotide triphosphate solutions provided in the Ambion® RETROscript® First Strand cDNA Synthesis Kit for RT-PCR (SKU# AM1710). They are subject to rigorous nuclease testing as well as a functional assay to ensure high levels of incorporation.

Orange DNA Loading Dye (6X) Thermo Scientific™

Thermo Scientific 6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis.

MassRuler DNA Loading Dye (6X) Thermo Scientific™

Thermo Scientific 6X MassRuler DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains one dye (bromophenol blue) for visual tracking of DNA migration during electrophoresis.

TriTrack DNA Loading Dye (6X) Thermo Scientific™

Thermo Scientific 6X TriTrack DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis.

X-Gluc Thermo Scientific™

Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate...

UltraPure™ BSA (50 mg/mL) Invitrogen™

UltraPure BSA is a non-acetylated bovine serum albumin (BSA) pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL. BSA has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions.

Pierce™ Endotoxin-Free Magnesium Chloride (MgCl₂) (10 mM) Thermo Scientific™

Pierce Endotoxin-Free MgCl2 is suitable for the preparation of samples for endotoxin detection (LAL) assay. As a diluent, it will eliminate the inhibitory chelation effects of EDTA. It contains <0.005 EU/mL endotoxin.

X-Gal Solution, ready-to-use Thermo Scientific™

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.

RNase Removal Reagent Thermo Scientific Chemicals

Destroys RNases and DNases on contactRNase removal reagent is used to remove the RNase from glasswares, plastic surfaces, equipments, gel boxes, benchtops and labware. It is also used to eliminate the RNase from microcentrifuge tubes without any side enzymatic reactions.