Miscellaneous Molecular Biology Reagents | Thermo Fisher Scientific

Agarose I (Molecular Biology Grade) Thermo Scientific™

Thermo Scientific Pierce Agaroses are highly purified and carefully blended formulations of regular- or low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.

NaCl (5 M), RNase-free Invitrogen™

Ambion® Molecular biology grade, 5 M NaCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

DTT (dithiothreitol) Thermo Scientific™

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro.

Tris Thermo Scientific™

Thermo Scientific Pierce Tris is a standard reagent for preparation of many molecular biology buffers. Features of Thermo Scientific Pierce Tris • ≥99.8% pure • White crystalline powder • Optimal buffering between pH 7 and 9

Glycerol Thermo Scientific™

Thermo Scientific Pierce Glycerol is high-purity glycerol (greater than 99% pure) for use in molecular biology methods.

SDS, 20% Solution, RNase-free Invitrogen™

Ambion® molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.

Phenol:Chloroform:IAA, 25:24:1, pH 6.6 Invitrogen™

Ambion® Acid Phenol:Chloroform:IAA (25:24:1) is premixed and supplied at pH 6.6 (pH 7.9 ± 0.2), with included buffer. Acid Phenol:Chloroform:IAA is provided in one bottle of 400 mL. In RNA and DNA extraction procedures, Acid Phenol:Chloroform:IAA (pH 7.

DNase I Buffer (10X) Invitrogen™

The same buffer provided with our Ambion® DNase I (AM2222, AM2224) and recombinant DNase I (AM2235) enzymes, offered here as a stand-alone product in case extra buffer is needed.

Orange DNA Loading Dye (6X) Thermo Scientific™

Thermo Scientific 6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis.

DNA Loading Dye & SDS Solution (6X) Thermo Scientific™

Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts.

RNA Gel Loading Dye (2X) Thermo Scientific™

Thermo Scientific 2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide.

Yeast DNA Extraction Kit Thermo Scientific™

Thermo Scientific Pierce Yeast DNA Extraction Reagent Kit utilizes Thermo Scientific Y-PER Reagent to quickly and easily lyse yeast cells to release DNA for study. Features of Yeast DNA Extraction Kit: • Eliminates need for glass beads or harsh enzyme treatments • Yields DNA that is compatible with...

Sodium Acetate Solution (3 M), pH 5.2 Thermo Scientific™

Thermo Scientific 3 M Sodium Acetate Solution, pH 5.2 is a 0.22 µm membrane filtered, sterile, nuclease-free solution of sodium acetate. This solution is routinely used in DNA and RNA precipitation. Applications • DNA precipitation • RNA precipitation

UltraPure™ Dithiothreitol Invitrogen™

UltraPure™ Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups. Higher concentrations of DTT are used to cleave disulfide linkages in polypeptides and to facilitate...

Dodecyl sulfate, sodium salt, 99%, for biochemistry Thermo Scientific Chemicals

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...

Polysorbate 20, Thermo Scientific Chemicals Thermo Scientific Chemicals

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...

Polysorbate 80, Thermo Scientific Chemicals Thermo Scientific Chemicals

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...

Phenol:Chloroform + Tris Buffer Thermo Scientific™

Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6.7 or pH 8.0. Features of Phenol:Chloroform + Tris Buffer : • Offers excellent, nuclease-free performance • Phenol is...

EDTA Thermo Scientific™

Thermo Scientific Pierce EDTA is useful as a chelator of alkaline earth metals, as well as iron, copper and zinc in a variety of laboratory methods.

Formamide Thermo Scientific™

Thermo Scientific Pierce Formamide is a high-purity formulation of formamide denaturant that is suitable for the preparation of hybridization cocktails and various denaturing solutions used with nucleic acids.

UltraPure™ Phenol Invitrogen™

UltraPure™ Phenol is used in the purification of nucleic acids. In a mixture of phenol and buffered aqueous solution, proteins are denatured and collect at the interphase while most nucleic acids remain in the aqueous phase. UltraPure™ Phenol contains no preservatives.

UltraPure™ Phenol:Water (3.75:1, v/v) Invitrogen™

UltraPure™ Phenol:Water (3.75:1, v/v) is used in the purification of nucleic acids. This liquid reagent consists of UltraPure™ Phenol and highly pure deionized water. The reagent can be used to prepare different types of buffer-saturated phenol.

UltraPure™ Guanidine Isothiocyanate Solution Invitrogen™

UltraPure™ Guanidine Isothiocyanate Solution consists of 4 M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA. It may be used as part of a protocol, based on the guanidine isothiocyanate procedure first described by Chirgwin et al. (1) and later modified by Han et al.

Wash Buffer A for Dynabeads™ mRNA Purification Kits Invitrogen™

This Wash Buffer A is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer A than is provided in the kit.

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU Invitrogen™

The APO‑BrdU TUNEL Assay Kit provides the materials necessary to label DNA strand breaks for the detection of apoptotic cells, as well as fixed samples of positive and negative control cells for assessing assay performance.

HATU Coupling Reagent Applied Biosystems™

This is a coupling reagent for use in peptide synthesis. For Research Use Only. Not for use in diagnostics procedures.

Carboxyl Latex Beads, 4% w/v, 0.02 µm Invitrogen™

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character,...

Carboxyl Latex Beads, 4% w/v, 2 µm Invitrogen™

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character,...

MitoProbe™ JC-1 Assay Kit Invitrogen™

The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry. View a selection guide for all apoptosis assays for flow cytometry. Selective to mitochondrial changes The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor...

dNTP Mix (2.5 mM) Invitrogen™

The same high-quality deoxynucleotide triphosphate solutions provided in the Ambion® RETROscript® First Strand cDNA Synthesis Kit for RT-PCR (SKU# AM1710). They are subject to rigorous nuclease testing as well as a functional assay to ensure high levels of incorporation.