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Miscellaneous Molecular Biology Reagents
These products include various reagents used in molecular biology applications.
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Agarose I (Molecular Biology Grade) Thermo Scientific™
Thermo Scientific Pierce Agaroses are highly purified and carefully blended formulations of regular- or low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.
NaCl (5 M), RNase-free Invitrogen™
Ambion® Molecular biology grade, 5 M NaCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
DTT (dithiothreitol) Thermo Scientific™
Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro.
SDS, 20% Solution, RNase-free Invitrogen™
Ambion® molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.
Tris Thermo Scientific™
Thermo Scientific Pierce Tris is a standard reagent for preparation of many molecular biology buffers. Features of Thermo Scientific Pierce Tris • ≥99.8% pure • White crystalline powder • Optimal buffering between pH 7 and 9
EDTA (0.5 M), pH 8.0 Thermo Scientific™
Thermo Scientific 0.5 M EDTA, pH 8.0 is an aqueous solution of 0.22 µm membrane-filtered ethylendiaminetetraacetic acid (EDTA), sodium salt. It's ideal for biochemistry or molecular biology applications that require a chelator for divalent cations.
Orange DNA Loading Dye (6X) Thermo Scientific™
Thermo Scientific 6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis.
DNA Loading Dye & SDS Solution (6X) Thermo Scientific™
Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts.
Yeast DNA Extraction Kit Thermo Scientific™
Thermo Scientific Pierce Yeast DNA Extraction Reagent Kit utilizes Thermo Scientific Y-PER Reagent to quickly and easily lyse yeast cells to release DNA for study. Features of Yeast DNA Extraction Kit: • Eliminates need for glass beads or harsh enzyme treatments • Yields DNA that is compatible with...
Phenol:Chloroform:IAA, 25:24:1, pH 6.6 Invitrogen™
Ambion® Acid Phenol:Chloroform:IAA (25:24:1) is premixed and supplied at pH 6.6 (pH 7.9 ± 0.2), with included buffer. Acid Phenol:Chloroform:IAA is provided in one bottle of 400 mL. In RNA and DNA extraction procedures, Acid Phenol:Chloroform:IAA (pH 7.
IPTG Solution, ready-to-use Thermo Scientific™
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization.
IPTG Invitrogen™
IPTG (isopropylthio-β-galactoside) is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures. How it works IPTG binds to the lac repressor and releases lac repressor from the lac operator, thereby allowing...
Triton|r X-100, Electrophoresis Reagent Thermo Scientific Chemicals
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo...
Carboxyl Latex Beads, 4% w/v, 2 µm Invitrogen™
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character,...
Poloxamer 188, Thermo Scientific Chemicals Thermo Scientific Chemicals
Poloxamer is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio.
RNase Removal Reagent Thermo Scientific Chemicals
Destroys RNases and DNases on contactRNase removal reagent is used to remove the RNase from glasswares, plastic surfaces, equipments, gel boxes, benchtops and labware. It is also used to eliminate the RNase from microcentrifuge tubes without any side enzymatic reactions.
Ethidium Bromide, Electrophoresis Reagent, 98% (dry weight) Thermo Scientific Chemicals
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo...
APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU Invitrogen™
The APO‑BrdU TUNEL Assay Kit provides the materials necessary to label DNA strand breaks for the detection of apoptotic cells, as well as fixed samples of positive and negative control cells for assessing assay performance.
GTP Soln. (10 mM) Invitrogen™
This Ambion® ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript® Kits (SKU#s AM1312, AM1316, AM1314, AM1326, AM1324, AM1318, AM1322, AM1320, AM1310, and AM1308) and is supplied in one tube containing 100 μL.
DNase I Buffer (10X) Invitrogen™
The same buffer provided with our Ambion® DNase I (AM2222, AM2224) and recombinant DNase I (AM2235) enzymes, offered here as a stand-alone product in case extra buffer is needed.
dNTP Mix (2.5 mM) Invitrogen™
The same high-quality deoxynucleotide triphosphate solutions provided in the Ambion® RETROscript® First Strand cDNA Synthesis Kit for RT-PCR (SKU# AM1710). They are subject to rigorous nuclease testing as well as a functional assay to ensure high levels of incorporation.
EDTA (0.5 M), pH 8.0, RNase-free Invitrogen™
Ambion® Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
Linear Acrylamide (5 mg/ml) (1 ml Tube) Invitrogen™
Ambion™ Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified. It is supplied in five tubes containing 1 mL each.
Potassium Acetate (3 M), pH 5.5, RNase-free Invitrogen™
Ambion® Molecular biology grade, 3 M Potassium Acetate, pH 5.5 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
Urea Invitrogen™
This high-purity molecular biology grade Ambion® urea is ideal for the preparation of denaturing polyacrylamide gels used in nuclease protection assays. Supplied in one bottle containing 1 kg. Urea is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
EDTA (0.1 mM), pH 8.0, RNase-free Invitrogen™
Ambion® 0.1 mM EDTA is a commonly recommended storage solution for RNA samples. 0.1 mM EDTA is in nuclease-free, ultrapure water. It is provided in one bottle of 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet.
ATP Solution (10 mM) Invitrogen™
This Ambion® ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript® Kits (SKU#s AM1312, AM1316, AM1314, AM1326, AM1324, AM1318, AM1322, AM1320, AM1310, and AM1308) and is supplied in one tube containing 100 μL.
UTP Soln. (10 mM) Invitrogen™
This Ambion® ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript® Kits (SKU#s AM1312, AM1316, AM1314, AM1326, AM1324, AM1318,#AM1322, AM1320, AM1310, and AM1308) and is supplied in one tube containing 100 μL.
Yeast RNA (10 mg/mL) Invitrogen™
Purified Torulla Ambion™ yeast RNA is suitable as a coprecipitant in nucleic acid precipitations. It cannot be used in reactions inhibited by exogenous RNA, and it is the most inexpensive source of a high-quality coprecipitant.
Yeast RNA (5 mg/ml) Invitrogen™
Purified Saccharomyces cerevisiae yeast RNA is suitable as a coprecipitant in nucleic acid precipitations. It cannot be used in reactions inhibited by exogenous RNA, and it is the most inexpensive source of a high-quality coprecipitant.
HATU Coupling Reagent Applied Biosystems™
This is a coupling reagent for use in peptide synthesis. For Research Use Only. Not for use in diagnostics procedures.
Wash Buffer A for Dynabeads™ mRNA Purification Kits Invitrogen™
This Wash Buffer A is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer A than is provided in the kit.
UltraPure™ Phenol Invitrogen™
UltraPure™ Phenol is used in the purification of nucleic acids. In a mixture of phenol and buffered aqueous solution, proteins are denatured and collect at the interphase while most nucleic acids remain in the aqueous phase. UltraPure™ Phenol contains no preservatives.
UltraPure™ Guanidine Isothiocyanate Solution Invitrogen™
UltraPure™ Guanidine Isothiocyanate Solution consists of 4 M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA. It may be used as part of a protocol, based on the guanidine isothiocyanate procedure first described by Chirgwin et al. (1) and later modified by Han et al.
UltraPure™ Phenol:Water (3.75:1, v/v) Invitrogen™
UltraPure™ Phenol:Water (3.75:1, v/v) is used in the purification of nucleic acids. This liquid reagent consists of UltraPure™ Phenol and highly pure deionized water. The reagent can be used to prepare different types of buffer-saturated phenol.
Formamide Thermo Scientific™
Thermo Scientific Pierce Formamide is a high-purity formulation of formamide denaturant that is suitable for the preparation of hybridization cocktails and various denaturing solutions used with nucleic acids.
EDTA Thermo Scientific™
Thermo Scientific Pierce EDTA is useful as a chelator of alkaline earth metals, as well as iron, copper and zinc in a variety of laboratory methods.
Glycerol Thermo Scientific™
Thermo Scientific Pierce Glycerol is high-purity glycerol (greater than 99% pure) for use in molecular biology methods.
Phenol:Chloroform + Tris Buffer Thermo Scientific™
Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6.7 or pH 8.0. Features of Phenol:Chloroform + Tris Buffer : • Offers excellent, nuclease-free performance • Phenol is...
UltraPure™ Dithiothreitol Invitrogen™
UltraPure™ Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups. Higher concentrations of DTT are used to cleave disulfide linkages in polypeptides and to facilitate...
Polysorbate 20, Thermo Scientific Chemicals Thermo Scientific Chemicals
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...
Dodecyl sulfate, sodium salt, 99%, for biochemistry Thermo Scientific Chemicals
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...
Polysorbate 80, Thermo Scientific Chemicals Thermo Scientific Chemicals
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...
Carboxyl Latex Beads, 4% w/v, 0.02 µm Invitrogen™
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character,...
MagMAX™ Lysis/Binding Solution Concentrate Invitrogen™
Ambion MagMAX Lysis⁄Binding Solution Concentrate is a component of the MagMAX isolation kits listed below that is made available here for separate purchase. Provided in one 100 mL bottle. This solution is the same formulation as the lysis buffer used in Cat. Nos.
Water-Saturated Phenol, pH 6.6 Invitrogen™
Ambion® Water Saturated Phenol is supplied premixed and at pH 6.6 (pH 7.9 ± 0.2, with included buffer). Water Saturated Phenol is used in RNA and DNA extraction procedures and is provided in one bottle of 400 mL.
Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) Invitrogen™
Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 400 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when...
Salmon Sperm DNA, sheared (10 mg/mL) Invitrogen™
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100–200 µg/mL. Using this product This sheared salmon sperm DNA has been treated with Proteinase K to remove any contaminating nucleases, followed by...
SDS, 10% Solution, RNase-free Invitrogen™
Molecular biology grade, Ambion® SDS, 10% Solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.
MitoProbe™ JC-1 Assay Kit Invitrogen™
The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry. View a selection guide for all apoptosis assays for flow cytometry. Selective to mitochondrial changes The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor...
IPTG, dioxane-free Thermo Scientific™
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization.
X-Gal Thermo Scientific™
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.
MassRuler DNA Loading Dye (6X) Thermo Scientific™
Thermo Scientific 6X MassRuler DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains one dye (bromophenol blue) for visual tracking of DNA migration during electrophoresis.
TriTrack DNA Loading Dye (6X) Thermo Scientific™
Thermo Scientific 6X TriTrack DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis.
5-Fluoroorotic Acid Thermo Scientific™
Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase.
MgCl2 (magnesium chloride) (25 mM) Thermo Scientific™
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 µm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR with Taq DNA polymerase, DreamTaq DNA Polymerase, Maxima Hot Start Taq DNA Polymerase, TrueStart Hot Start Taq DNA Polymerase, Long PCR...
Aminoallyl-UTP Solution (50 mM) Thermo Scientific™
Thermo Scientific Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH. Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases.
Wash Buffer B for Dynabeads™ mRNA Purification Kits Invitrogen™
This Wash Buffer B is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61006, 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer B than is provided in the kit.
UltraPure™ BSA (50 mg/mL) Invitrogen™
UltraPure BSA is a non-acetylated bovine serum albumin (BSA) pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL. BSA has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions.
Fast Green FCF, Electrophoresis Reagent Thermo Scientific Chemicals
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo...
