Miscellaneous Molecular Biology Reagents

These products include various reagents used in molecular biology applications.
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Ambion Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 μm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR with Taq DNA polymerase, DreamTaq DNA Polymerase, Maxima Hot Start Taq DNA Polymerase, TrueStart Hot Start Taq DNA Polymerase, Long PCR...
dNTP Mix (2.5 mM) Invitrogen™
The same high-quality deoxynucleotide triphosphate solutions provided in the Ambion® RETROscript® First Strand cDNA Synthesis Kit for RT-PCR (SKU# AM1710). They are subject to rigorous nuclease testing as well as a functional assay to ensure high levels of incorporation.
This elution buffer is included in the MagMAX-96 Total RNA Isolation Kit (Cat. No. AM1830) and MagMAX-96 Viral RNA Isolation Kit (AM1836). It is made available here for separate purchase.
The MitoProbe™ JC-1 Assay Kit is a quick and reliable assay used to detect changes in mitochondria by flow cytometry. View a selection guide for all apoptosis assays for flow cytometry. Selective to mitochondrial changes The membrane-permeant JC-1 dye is widely used in apoptosis studies to monitor...
The same buffer provided with our Ambion® DNase I (AM2222, AM2224) and recombinant DNase I (AM2235) enzymes, offered here as a stand-alone product in case extra buffer is needed.
EDTA, Concentrate Thermo Scientific™
This EDTA solution is specifically formulated for use in our Heat Induced Epitope Retrieval (HIER) method. Supplied as a 100 mL aliquot of 20X concentrate or 1L ready-to-use format.
Ambion® 0.1 mM EDTA is a commonly recommended storage solution for RNA samples. 0.1 mM EDTA is in nuclease-free, ultrapure water. It is provided in one bottle of 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet.
Thermo Scientific Pierce Agaroses are highly purified and carefully blended formulations of regular- or low-melting agarose that provide uniform lot-to-lot consistency for preparation of electrophoresis gels.
Galactosidase activation reagent used in recombinant microbial fermentation processes. Made with non-animal derived materials.
XTT, GMP BIOVECTRA
XTT, widely used to measure cell proliferation in toxicity assays.
The APO‑BrdU TUNEL Assay Kit provides the materials necessary to label DNA strand breaks for the detection of apoptotic cells, as well as fixed samples of positive and negative control cells for assessing assay performance.
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.
Thermo Scientific Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH. Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases.
This Wash Buffer B is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61006, 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer B than is provided in the kit.
PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA. This reagent is also a component of the PureLink™ plasmid purification system. Concentration 20 mg RNase A/mL in 50 mM Tris-HCl (pH 8.0), 10 mM EDTA.
UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C. Because it has a density of 1.26 g/mL, glycerol frequently is used as a component in electrophoresis loading buffers.
UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations. It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1).
IPTG Invitrogen™
IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.
UltraPure™ Guanidine Hydrochloride is a strong protein denaturant. In a highly concentrated (5 to 6 M) guanidine hydrochloride solution, many proteins unfold and separate into their constituent polypeptide chains. Guanidine hydrochloride is widely used to purify nucleic acids from cell extracts.
UltraPure™ Cesium Chloride, Optical Grade is specifically prepared for use in density gradients. The highest grade commercially available, UltraPure™ Cesium Chloride, Optical Grade can be used to prepare plasmids or to isolate phage, virus particles, or cellular RNA.
UltraPure™ Sucrose is suitable for forming density gradients for a variety of separation applications. It is used to separate RNAs, to prepare bacteriophage λ-DNA arms, and to separate proteins. UltraPure™ Sucrose gradients typically are formed by use of gradient formers or by layering two or more...
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
UltraPure™ Formamide is commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron microscopy, and hybridization. A liquid at room temperature, UltraPure™ Formamide is vacuum distilled and packaged under dry nitrogen and can be used as supplied for many applications...
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character,...
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character,...
Yeast DNA Extraction Kit Thermo Scientific™
Thermo Scientific Pierce Yeast DNA Extraction Reagent Kit utilizes Thermo Scientific Y-PER Reagent to quickly and easily lyse yeast cells to release DNA for study. Features of Yeast DNA Extraction Kit: • Eliminates need for glass beads or harsh enzyme treatments • Yields DNA that is compatible with...
Ambion formamide has been deionized, is nuclease-free, and comes packaged as a liquid in an amber glass bottle under argon. Supplied in 5 bottles containing 500 g each. Formamide lowers the melting temperature of nucleic acid duplexes and is widely used in applications such as Southern and northern...
Ambion™ Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10–20 μg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids. It is supplied in one tube containing 0.5 mL at a concentration of 10 mg/mL. What is a Coprecipitant?
CTP Soln. (10 mM) Invitrogen™
This Ambion® solution is a component of the MAXIscript® Kits (SKU#s AM1312, AM1316, AM1314, AM1326, AM1324, AM1318, AM1322, AM1320, AM1310, and AM1308) and is supplied in one tube containing 100 μL. Each is subjected to RNase testing as well as a functional assay to ensure high levels of...
MgCl2 (1 M) Invitrogen™
Molecular biology grade, Ambion™ 1 M MgCl2 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g.
UltraPure BSA is a non-acetylated bovine serum albumin (BSA) pure enough to use when DNA or RNA integrity is critical. One tube containing 50 mg is provided, at a concentration of 50 mg/mL. BSA has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions.
Ambion® MagMAX™ Wash Solution 1 Concentrate is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one 205 mL bottle.
Ambion® MagMAX™ Wash Solution 2 Concentrate is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one 200 mL bottle.
Ambion® Molecular biology grade, 2 M KCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
Ambion® Molecular biology grade, 3 M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
IPTG (Dioxane-Free) Invitrogen™
Ambion IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon.
Ambion Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 500 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
Ambion Molecular biology grade, 5 M NaCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
Molecular biology grade, Ambion 20X SSC solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.
This Lysis/Binding Buffer is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61011, 61012, and 61021. It is made available separately for applications that require more Lysis/Binding Buffer than is provided in the kit.
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character,...
Premium cGMP grade dithiothreitol includes additional testing for residual solvents.
Premium cGMP grade reagent used in the reduction of disulfide bonds, and during prep of ADCs.
Thermo Scientific Pierce D-Luciferin, Monosodium Salt is formulated at greater than 99% purity to provide convenience and high performance at a low cost in reporter assays involving firefly luciferase. Features of D-Luciferin, Monosodium Salt: • Molecular mass = 318.
Thermo Scientific Pierce D-Luciferin, Monopotassium Salt is formulated at greater than 99% purity to provide convenience and high performance at a low cost in reporter assays involving firefly luciferase. Features of D-Luciferin, Monopotassium Salt: • Molecular mass = 318.
RNA Gel Loading Dye (2X) Thermo Scientific™
Thermo Scientific 2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide.
Orange DNA Loading Dye (6X) Thermo Scientific™
Thermo Scientific 6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis.
DTT (dithiothreitol) Thermo Scientific™
Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro.
X-Gal Solution, ready-to-use Thermo Scientific™
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.
Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts.
Thermo Scientific 3 M Sodium Acetate Solution, pH 5.2 is a 0.22 μm membrane filtered, sterile, nuclease-free solution of sodium acetate. This solution is routinely used in DNA and RNA precipitation. Applications • DNA precipitation • RNA precipitation
Thermo Scientific GeneJET Wash Solution I (concentrated) is a component of the GeneJET Plasmid Maxiprep Kit (Cat. No. K0491, K0492) and may be purchased separately. Provided in 360 mL aliquots.
X-Gluc Thermo Scientific™
Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate...
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo...
HATU Coupling Reagent Applied Biosystems™
This is a coupling reagent for use in peptide synthesis. For Research Use Only. Not for use in diagnostics procedures.
This Wash Buffer A is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer A than is provided in the kit.
Triton|r X-100, Electrophoresis Reagent Thermo Scientific Chemicals
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo...
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With 35+ years of experience in designing, developing, and manufacturing paramagnetic beads to internationally recognized quality standards, our Dynabeads OEM team has collaborated with leading biotech, pharma, diagnostic, and healthcare companies.
Our Thermo Scientific molecular biology products are designed to deliver reproducibility and performance in everyday applications including reverse transcription, PCR, nucleic acid electrophoresis, restriction enzyme digests, and cloning.
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Can I transfect my primary cells?
Can I transfect my primary cells?