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Miscellaneous Molecular Biology Reagents
These products include various reagents used in molecular biology applications.
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IPTG Invitrogen™
IPTG (isopropylthio-β-galactoside) is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures. How it works IPTG binds to the lac repressor and releases lac repressor from the lac operator, thereby allowing...
Guanidine Isothiocyanate Invitrogen™
UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations. It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1).
Wash Buffer B for Dynabeads™ mRNA Purification Kits Invitrogen™
This Wash Buffer B is included in the following Dynabeads™ mRNA purification kits: Cat. Nos. 61006, 61011, 61012, and 61021. It is made available separately for applications that require more Wash Buffer B than is provided in the kit.
PureLink™ RNase A (20 mg/mL) Invitrogen™
PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA. This reagent is also a component of the PureLink™ plasmid purification system. Concentration 20 mg RNase A/mL in 50 mM Tris-HCl (pH 8.0), 10 mM EDTA.
Triton™ X-100, 98%, for molecular biology, DNAse, RNAse and Protease free Thermo Scientific Chemicals
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...
Pierce™ D-Luciferin, Monopotassium Salt Thermo Scientific™
Thermo Scientific Pierce D-Luciferin, Monopotassium Salt is formulated at greater than 99% purity to provide convenience and high performance at a low cost in reporter assays involving firefly luciferase. Features of D-Luciferin, Monopotassium Salt: • Molecular mass = 318.
Pierce™ D-Luciferin, Monopotassium Salt Thermo Scientific™
Thermo Scientific Pierce D-Luciferin, Monosodium Salt is formulated at greater than 99% purity to provide convenience and high performance at a low cost in reporter assays involving firefly luciferase. Features of D-Luciferin, Monosodium Salt: • Molecular mass = 318.
X-Gal Thermo Scientific™
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.
TriTrack DNA Loading Dye (6X) Thermo Scientific™
Thermo Scientific 6X TriTrack DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis.
IPTG, dioxane-free Thermo Scientific™
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization.
IPTG Solution, ready-to-use Thermo Scientific™
Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization.
MassRuler DNA Loading Dye (6X) Thermo Scientific™
Thermo Scientific 6X MassRuler DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains one dye (bromophenol blue) for visual tracking of DNA migration during electrophoresis.
5-Fluoroorotic Acid Thermo Scientific™
Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase.
MgCl2 (magnesium chloride) (25 mM) Thermo Scientific™
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 µm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR with Taq DNA polymerase, DreamTaq DNA Polymerase, Maxima Hot Start Taq DNA Polymerase, TrueStart Hot Start Taq DNA Polymerase, Long PCR...
Aminoallyl-UTP Solution (50 mM) Thermo Scientific™
Thermo Scientific Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH. Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases.
