Miscellaneous Molecular Biology Reagents | Thermo Fisher Scientific

UltraPure™ Formamide Invitrogen™

UltraPure™ Formamide is commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron microscopy, and hybridization. A liquid at room temperature, UltraPure™ Formamide is vacuum distilled and packaged under dry nitrogen and can be used as supplied for many applications...

Guanidine Isothiocyanate Invitrogen™

UltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations. It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1).

Guanidine Thiocyanate Invitrogen™

Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g.

KCl (2 M), RNase-free Invitrogen™

Ambion® Molecular biology grade, 2 M KCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

Yeast tRNA (10 mg/mL) Invitrogen™

Ambion™ Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10–20 µg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids. It is supplied in one tube containing 0.5 mL at a concentration of 10 mg/mL. What is a Coprecipitant?

Ammonium Acetate (5 M), RNase-free Invitrogen™

Ambion® Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 500 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

X-Gal Solution, ready-to-use Thermo Scientific™

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.

Wash Solution I (concentrated) Thermo Scientific™

Thermo Scientific GeneJET Wash Solution I (concentrated) is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 360 mL aliquots.

X-Gluc Thermo Scientific™

Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate...

PureLink™ RNase A (20 mg/mL) Invitrogen™

PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA. This reagent is also a component of the PureLink™ plasmid purification system. Concentration 20 mg RNase A/mL in 50 mM Tris-HCl (pH 8.0), 10 mM EDTA.

UltraPure™ Guanidine Hydrochloride Invitrogen™

UltraPure™ Guanidine Hydrochloride is a strong protein denaturant. In a highly concentrated (5 to 6 M) guanidine hydrochloride solution, many proteins unfold and separate into their constituent polypeptide chains. Guanidine hydrochloride is widely used to purify nucleic acids from cell extracts.

UltraPure™ Cesium Chloride, Optical Grade Invitrogen™

UltraPure™ Cesium Chloride, Optical Grade is specifically prepared for use in density gradients. The highest grade commercially available, UltraPure™ Cesium Chloride, Optical Grade can be used to prepare plasmids or to isolate phage, virus particles, or cellular RNA.

UltraPure™ Buffer-Saturated Phenol Invitrogen™

UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.

UltraPure™ Urea Invitrogen™

UltraPure™ Urea is an effective protein denaturant. In a highly concentrated (6 to 8 M) urea solution, many proteins unfold and separate into their constituent polypeptide chains. UltraPure™ Urea is also used to denature nucleic acids in sequencing gels.

IPTG Invitrogen™

IPTG (isopropylthio-β-galactoside) is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures. How it works IPTG binds to the lac repressor and releases lac repressor from the lac operator, thereby allowing...