Shop All RNAi and RNA Reagents

For Use With (Equipment)


Number of Arrays


Purification Target


Ambion™ In Vivo GAPDH Positive Control siRNA Invitrogen™

Ambion® In Vivo siRNA Controls
• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability
• For use in human, mouse, and rat cells

What are Ambion® In Vivo siRNAs?
Ambion® In Vivo siRNAs are designed using the proven Silencer® Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion® In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion® In Vivo siRNAs exhibit equivalent or better potency than Silencer® Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

GAPDH Positive Control siRNAs
Our extensively validated, positive control siRNA to human, mouse, and rat GAPDH serves multiple functions. First, it is an ideal “test" siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. In addition, because it targets GAPDH mRNA, which is commonly used as an internal control, its effects are easy to assay, and thus provides an excellent tool to monitor siRNA transfection efficiency by real-time RT-PCR. The Ambion® In Vivo GAPDH Positive Control siRNA shows increased serum and nuclease resistance compared with Silencer® Select siRNAs without loss of potency or specificity and is recommended for in vivo applications.

Quality Control
Ambion® synthesizes and purifies each Ambion® In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls
Our "In Vivo Ready" Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.

Ambion™ In Vivo Negative Control #1 siRNA Invitrogen™

Ambion® In Vivo siRNA Controls
• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability
• For use in human, mouse, and rat cells

What are Ambion® In Vivo siRNAs?
Ambion® In Vivo siRNAs are designed using the proven Silencer® Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion® In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion® In Vivo siRNAs exhibit equivalent or better potency than Silencer® Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Ambion® In Vivo siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these negative control siRNA has been tested in multi-parametric cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.

Quality Control
Ambion® synthesizes and purifies each Ambion® In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls
Our "In Vivo Ready" Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.

Anti-miR™ miRNA Inhibitor Invitrogen™

miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.

Ambion™ In Vivo GAPDH Positive Control siRNA Invitrogen™

Ambion® In Vivo siRNA Controls
• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability
• For use in human, mouse, and rat cells

What are Ambion® In Vivo siRNAs?
Ambion® In Vivo siRNAs are designed using the proven Silencer® Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion® In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion® In Vivo siRNAs exhibit equivalent or better potency than Silencer® Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

GAPDH Positive Control siRNAs
Our extensively validated, positive control siRNA to human, mouse, and rat GAPDH serves multiple functions. First, it is an ideal “test" siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. In addition, because it targets GAPDH mRNA, which is commonly used as an internal control, its effects are easy to assay, and thus provides an excellent tool to monitor siRNA transfection efficiency by real-time RT-PCR. The Ambion® In Vivo GAPDH Positive Control siRNA shows increased serum and nuclease resistance compared with Silencer® Select siRNAs without loss of potency or specificity and is recommended for in vivo applications.

Quality Control
Ambion® synthesizes and purifies each Ambion® In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls
Our "In Vivo Ready" Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.

Custom Anti-miR™ miRNA Inhibitor Invitrogen™

Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. Use our customization tool to design the ideal inhibitor for your miRNA sequence, or select from among predesigned products.

Custom RT Pools Thermo Scientific™

Custom miRNA array designs can hold from 12 to 380 assays, and 1 to 8 samples on each array design. Ten formats are offered, available only with microfluidics card design.

Poly(A) Tail-Length Assay Kit Thermo Scientific™

Newly reconfigured kit for increased efficiency: 76455 1 KT Poly(A) Tail-Length Assay Kit is a direct replacement for 76450 1 KT

• Measure the poly(A) tail of multiple RNAs in under 3 hrs using common lab equipment
• Accurate measurement of poly(A) tail length compared to other assays (e.g. northern blotting)
• One kit includes optimized reagents for 5 G/I Tailing, 20 RT and 80 PCR reactions

Description:
Poly adenylation of eukaryotic mRNAs plays a critical role in RNA metabolism including stability, enhanced translation, nuclear export and miRNA mediated gene regulation. The Poly(A) Tail-Length Assay Kit allows you to quickly measure the poly(A) tails of multiple mRNAs in only three hours. This kit utilizes a novel G/I extension technique which preserves the 3' end of RNAs and ensures accurate measurement of poly(A) tail-length. And, all reaction components have been optimized so you get quality data quickly.

Description:
The Poly(A) Tail-Length Assay Kit uses four key steps to enable poly(A) tail-length determination (Fig. 1).

In Step 1, poly(A) polymerase adds a limited number of guanosine and inosine residues to the 3’-ends of poly(A)-containing RNAs.

In Step 2, the tailed-RNAs are converted to DNA through reverse transcription using the newly added G/I tails as the priming sites.

In Step 3, PCR amplification products are generated using two primer sets. A gene-specific forward and reverse primer set designed upstream of the polyadenylation site (e.g. the 3’-UTR) is produced as a control for the gene-of-interest. The second set of primers uses the gene-specific forward primer and the universal reverse primer provided with the kit to generate a product that includes the poly(A) tails of the gene-of-interest.

Finally, in Step 4, the PCR products are separated on an agarose or polyacrylamide gel (Fig. 2).

Kit components:
Kit contains all necessary reagents for 5 G/I Tailing, 20 reverse transcription, and 80 PCR reactions.

Selected References:
Parker, R. and Song, H. (2004) Nat Struct Mol Biol. 11 121-127.
Brodersen, D.E. et al.  (2008) Biochim Biophys Acta. 1779, 532-537.
Wu, L., Fan J. and Belasco, J.G. (2006) Proc Natl Acad Sci U S A. 103, 4034-4039.
Izaurralde, E. et al. (2009) RNA 15, 21-32.
Martin, G. and Keller, W. (1998) RNA 4, 226-230.
Gauss-Müller, V. et al. (2001) Nucleic Acids Res. 29 E57-7

Ambion™ In Vivo Factor VII Positive Control siRNA Invitrogen™

Ambion® In Vivo siRNA Controls
• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability
• For use in mouse cells

What are Ambion® In Vivo siRNAs?
Ambion® In Vivo siRNAs are designed using the proven Silencer® Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion® In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion® In Vivo siRNAs exhibit equivalent or better potency than Silencer® Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

Factor VII (FVII) Positive Control siRNA
Ambion® In Vivo Factor VII siRNA, In Vivo Ready, provides a positive control for experiments involving Ambion® In Vivo siRNA delivery to mice. Factor VII (FVII⁄F7; also known as proconvertin) is a vitamin K-dependent serine protease that functions as a central protein in the coagulation cascade. FVII is synthesized in the liver and secreted to the plasma where it circulates in an inactive form. After delivery of FVII-targeting siRNA to the liver, knockdown can be measured by quantifying FVII protein levels in blood by robust, commercially available chromogenic assays. Moreover, blood samples can be collected from the same animal at multiple time points post-siRNA injection. These features enable much more rapid and efficient knockdown measurements for FVII than for gene targets that require quantitation of mRNA levels in liver tissue, making FVII an ideal control for in vivo siRNA delivery.

Quality Control
Ambion® synthesizes and purifies each Ambion® In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls
Our "In Vivo Ready" Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.

Customer-Defined Anti-miR™ miRNA Inhibitor Invitrogen™

Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. Use our customization tool to design the ideal inhibitor for your miRNA sequence, or select from among predesigned products.

Silencer™ Pre-Designed siRNA Invitrogen™

Silencer® Select siRNAs are classic 21-mers which incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemistry. Silencer® Select Pre-Designed siRNAs are guaranteed-to-silence based on their proven design.

Silencer™ Select Human Transcription Factor siRNA Library Invitrogen™

Transcription factors are fast becoming promising drug targets, as they bind to and regulate the transcription of genes. The Silencer™ Select Human Transcription Factor siRNA Library targets genes expressing proteins that bind DNA in a sequence-specific manner to control the rate of transcription of genetic information from DNA to messenger RNA. The Silencer Select Human Transcription Factor siRNA Library contains 5457 Ambion™ Silencer Select highly potent and chemically-modified siRNAs targeting 1819 known transcription factors. This library was designed using the most up-to-date genome databases, including the NCBI RefSeq database and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The Silencer Select siRNAs are designed using Thermo Fisher Scientific’s leading design algorithms—along with our novel locked nucleic acid (LNA) chemical modifications, this helps to maximize the efficacy, specificity and potency required for RNAi screening against transcription factors.

• Superior efficacyReduce off target effects by up to 90% due to latest design algorithms and LNA chemical modifications
• Highest efficiency—Perform knockdown studies with 100-fold more potent siRNAs than currently available siRNAs
• Dependable—Achieve faster discoveries via consistency of gene knockdown and reliability of phenotypic results
• Convenient—Reduce data de-convolution and accelerate target validation with our arrayed format
• Customizable—Choose the best option for your workflow demands, including additional siRNA scales and pooled formats

Why use the Silencer Select Human Transcription Factor siRNA Library?
Together with careful bioinformatics selection of gene targets, Silencer Select siRNAs are optimized with latest design algorithms, proprietary chemical modifications and high quality synthesis to help ensure desired RNAi outcomes (learn more). The Silencer Select Human Transcription Factor siRNA Library contains our pre-designed and when available, validated siRNAs generated with highest potency and specificity in mind so you can focus on the highest data quality backed by the best in industry guarantee (learn more about the guarantee).

Silencer Select siRNA libraries empower you with highly potent siRNAs to maximize discovery with even less
Ambion Silencer Select siRNAs are designed with the highest potency of available siRNAs, resulting in 100-fold more potent siRNAs and allowing gene knockdowns at the lowest concentrations (see figure below). The highly potent Silencer Select siRNAs allow you to use even lower concentrations of siRNAs, which helps to minimize off target effects, reduce false negative data and maximize siRNA library output for additional screens and data validation. The Silencer Select Human Transcription Factor siRNA Library contains 0.25 nmol of each highly potent siRNA, sufficient for at least 500 transfections (at 5 nM siRNA, 100 µL transfection volume).

Flexibility to meet your workflow needs
Thermo Fisher Scientific offers comprehensive list of pre-defined or custom siRNA libraries to human, mouse or rat genes with 1, 2, or 5 nmol of each siRNA or larger sizes. For a complete gene list, additional information or to discuss your research with one of our technical experts, contact your local sales representative or e-mail us at RNAilibraries@lifetech.com.

Non-Standard Custom Silencer™ siRNA, Custom Size & Purity Invitrogen™

RNA interference (RNAi) is the best way to effectively knock down gene expression to study protein function in a wide range of cell types. Use our GeneAssist™ Custom siRNA Builder to design and order a custom siRNA.

LentiArray™ CRISPR Negative Control Lentivirus, human, non-targeting, with GFP Invitrogen™

Negative control gRNAs are essential in screening applications for setting thresholds for hit determination. The Invitrogen™ LentiArray™ CRISPR Negative Control Lentivirus, Human Scrambled, contains a gRNA sequence with no sequence homology to any region of the human genome. This construct also expresses emGFP to provide a visual readout of successful transduction that can be used in determining Multiplicity of Infection (MOI).

Singleplex miRNA 200rxn Thermo Scientific™

Singleplex miRNA 200rxn

Breast Adenocaricinoma (MCF-7) Total RNA Invitrogen™

Breast Adenocarcinoma (MCF-7) Total RNA is a total RNA sample extracted from MCF-7 cells for use as a positive control in QuantiGene assays.
Results per page
    spinner