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mirVana™ miRNA Inhibitor, let-7c positive control Invitrogen™
miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.
We have now released the second generation of miRNA inhibitors, mirVana™ miRNA Inhibitors, which:
• Display better potency than lead competitor's product
• Offer maximum and consistent effect in vitro at low miRNA inhibitor concentration
• Are non toxic
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering consistency throughout your entire research project
Positive and negative controls are also available for use along with mirVana™ miRNA Inhibitors in miRNA down-regulation experiments.
mirVana™ miRNA Inhibitor let-7c Positive Control
The mirVana™ miRNA Inhibitor let-7c Positive Control is intended for use as a positive control for mammalian cell culture experiments using mirVana™ miRNA Inhibitors. Endogenous let-7c miRNA has been shown to negatively regulate HMGA2 mRNA in cultured cells. HMGA2 is a ubiquitously expressed, nonhistone, chromatin protein that can modulate gene expression through changes in chromatin architecture. let-7c miRNA down-regulates levels of HMGA2 mRNA. When transfected into human and mouse cell lines, let-7c miRNA inhibitor blocks endogenous let-7c miRNA, resulting in increased levels of HMGA2 mRNA. Thus, let-7c miRNA inhibitor activity can be monitored in human or mouse cells using real-time reverse transcription PCR (RT-PCR) to detect HMGA2 mRNA.
First Generation Inhibitor Controls
Our first-generation miRNA inhibitors (and controls), Ambion® Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Inhibitors. These first-generation inhibitors are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Inhibitors and their corresponding controls.
We have now released the second generation of miRNA inhibitors, mirVana™ miRNA Inhibitors, which:
• Display better potency than lead competitor's product
• Offer maximum and consistent effect in vitro at low miRNA inhibitor concentration
• Are non toxic
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering consistency throughout your entire research project
Positive and negative controls are also available for use along with mirVana™ miRNA Inhibitors in miRNA down-regulation experiments.
mirVana™ miRNA Inhibitor let-7c Positive Control
The mirVana™ miRNA Inhibitor let-7c Positive Control is intended for use as a positive control for mammalian cell culture experiments using mirVana™ miRNA Inhibitors. Endogenous let-7c miRNA has been shown to negatively regulate HMGA2 mRNA in cultured cells. HMGA2 is a ubiquitously expressed, nonhistone, chromatin protein that can modulate gene expression through changes in chromatin architecture. let-7c miRNA down-regulates levels of HMGA2 mRNA. When transfected into human and mouse cell lines, let-7c miRNA inhibitor blocks endogenous let-7c miRNA, resulting in increased levels of HMGA2 mRNA. Thus, let-7c miRNA inhibitor activity can be monitored in human or mouse cells using real-time reverse transcription PCR (RT-PCR) to detect HMGA2 mRNA.
First Generation Inhibitor Controls
Our first-generation miRNA inhibitors (and controls), Ambion® Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Inhibitors. These first-generation inhibitors are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Inhibitors and their corresponding controls.
FAM3™ Dye-Labeled Pre-miR Negative Control #1 Invitrogen™
Ambion® FAM™ dye-labeled synthetic miRNA mimic for miRNA functional analysis. 5 nmol is supplied.
• Optimized design results in correct strand utilization by RISC
• Purified and ready to transfect
Ambion® Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments. The FAM™ Dye-Labeled Pre-miR™ Negative Control #1 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is ideal for monitoring transfection efficiency, as the fluorescent label enables direct observation of the cellular uptake, distribution, and localization of the control. The label is on the 5' end of the molecule, and the oligonucleotide sequence is the same as the Pre-miR™ miRNA Precursor Molecule Negative Control #1 (SKU #AM17110). NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
• Optimized design results in correct strand utilization by RISC
• Purified and ready to transfect
Ambion® Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments. The FAM™ Dye-Labeled Pre-miR™ Negative Control #1 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is ideal for monitoring transfection efficiency, as the fluorescent label enables direct observation of the cellular uptake, distribution, and localization of the control. The label is on the 5' end of the molecule, and the oligonucleotide sequence is the same as the Pre-miR™ miRNA Precursor Molecule Negative Control #1 (SKU #AM17110). NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
Anti-miR™ hsa-let-7c miRNA Inhibitor Positive Control Invitrogen™
Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Anti-miR™ let-7c miRNA Inhibitor Positive Control provides a convenient, validated positive control for experiments using Anti-miR miRNA Inhibitors. Endogenous let-7 miRNA negatively regulates HMGA2, a ubiquitously expressed, nonhistone chromatin protein that modulates gene expression through changes in chromatin architecture. When transfected into human and mouse cells, Anti-miR hsa-let-7c miRNA Inhibitor blocks endogenous let-7c miRNA, resulting in increased levels of HMGA2 mRNA.
Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. Anti-miR™ miRNA Inhibitor Negative Control #1 (SKU #AM17010) is a random sequence that has been validated to produce no identifiable effects on known miRNA function. Cy™3 and FAM™ dye-labeled Anti-miR Negative Controls (SKU #AM17011 and #AM17012, respectively) are also available. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.
Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. Anti-miR™ miRNA Inhibitor Negative Control #1 (SKU #AM17010) is a random sequence that has been validated to produce no identifiable effects on known miRNA function. Cy™3 and FAM™ dye-labeled Anti-miR Negative Controls (SKU #AM17011 and #AM17012, respectively) are also available. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.
Silencer™ Select Negative Control No. 2 siRNA Invitrogen™
Ambion® Silencer® SelectNegative Control #2 siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 40 nmol is provided.
• Premium-quality siRNA, highly purified and ready to use
• Functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells
What are Silencer® Select siRNAs?
Silencer® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Silencer® Select Negative Control #2 siRNA:
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Silencer® Select Negative Control #2 siRNA has been carefully designed and extensively tested, and has no significant sequence similarity to mouse, rat, or human gene sequences. This negative control siRNA has been tested by microarray analysis and shown to have minimal effects on gene expression. It has also been tested in multi-parametric, cell-based assays and shown to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.
Quality Control:
Silencer® Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.
Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer® Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer® Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion® Silencer® siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
• Premium-quality siRNA, highly purified and ready to use
• Functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells
What are Silencer® Select siRNAs?
Silencer® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Silencer® Select Negative Control #2 siRNA:
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Silencer® Select Negative Control #2 siRNA has been carefully designed and extensively tested, and has no significant sequence similarity to mouse, rat, or human gene sequences. This negative control siRNA has been tested by microarray analysis and shown to have minimal effects on gene expression. It has also been tested in multi-parametric, cell-based assays and shown to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.
Quality Control:
Silencer® Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.
Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer® Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer® Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion® Silencer® siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
Pre-miR™ hsa-miR-1 miRNA Precursor Postive Control Invitrogen™
The Pre-miR™ hsa-miR-1 miRNA Precursor is intended for use as a positive control in experiments utilizing Pre-miR miRNA Precursors. This precursor was chosen as a positive control because, upon delivery into cells, it effectively down regulates the expression of twinfilin-1, also known as PTK9, at the mRNA level (60-95% reduction. Effective delivery and activity of the Pre-miR hsa-miR-1 miRNA Precursor can be detected by real-time PCR using a TaqMan® Gene Expression Assay to PTK9 (Assay IDs: Hs00702289_s1 [human], Mm01598980_g1 [mouse]).
About Pre-miR miRNA Precursors
Pre-miR™ miRNA Precursor Molecules are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNAs. These ready-to-use miRNA mimics, which are similar to, but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments (Figure 1). Positive and negative control Pre-miR miRNA Precursors (listed above) or the Pre-miR™ Precursor Starter Kit can be used to optimize transfection of Pre-miR miRNA Precursors.
Pre-miR miRNA Precursor Molecules have been carefully designed and modified to ensure that the correct strand, representing the desired mature miRNA, is taken up into the RNA-induced silencing-like complex responsible for miRNA activity (Figure 2). In contrast to miRNA expression vectors, these synthetic molecules can be used in dose response studies due to their introduction directly into the cell by transfection or electroporation. Thanks to their small size, they are easier to transfect than vectors, and can be delivered using conditions identical to those used for siRNAs.
About Pre-miR miRNA Precursors
Pre-miR™ miRNA Precursor Molecules are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNAs. These ready-to-use miRNA mimics, which are similar to, but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments (Figure 1). Positive and negative control Pre-miR miRNA Precursors (listed above) or the Pre-miR™ Precursor Starter Kit can be used to optimize transfection of Pre-miR miRNA Precursors.
Pre-miR miRNA Precursor Molecules have been carefully designed and modified to ensure that the correct strand, representing the desired mature miRNA, is taken up into the RNA-induced silencing-like complex responsible for miRNA activity (Figure 2). In contrast to miRNA expression vectors, these synthetic molecules can be used in dose response studies due to their introduction directly into the cell by transfection or electroporation. Thanks to their small size, they are easier to transfect than vectors, and can be delivered using conditions identical to those used for siRNAs.
Pre-miR™ miRNA Precursor Negative Control #1 Invitrogen™
Pre-miR™ miRNA Precursors are designed to mimic endogenous miRNAs. Pre-miR miRNA Precursor Negative Control #1 is designed to use as a negative control for experiments using Pre-miR miRNA Precursors. Transfect Pre-miR miRNA Precursor Negative Control #1 using the same methodology as for your experimental Pre-miR miRNA Precursors, then use the target gene expression from the sample transfected with the Pre-miR miRNA Precursor Negative Control #1 as a baseline for evaluation of the effect of the experimental Pre-miR miRNA Precursor on target gene expression. Advantages of the Pre-miR™ miRNA Precursor Negative Control #1:
• Optimized design results in correct strand utilization by RISC
• Purified and ready to transfect
About Pre-miR miRNA Precursors
Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments.
Using Pre-miR miRNA Precursor Negative Control #1
Pre-miR™ Negative Control #1 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. RNA oligonucleotides are susceptible to degradation by exogenous ribonucleases introduced during handling. Wear gloves when handling this product. Use RNase-free reagents, tubes, and barrier pipette tips. Upon receipt, store in a non-frost-free freezer at or below -20°C (dried oligonucleotides are shipped at ambient temperature).
NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
• Optimized design results in correct strand utilization by RISC
• Purified and ready to transfect
About Pre-miR miRNA Precursors
Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments.
Using Pre-miR miRNA Precursor Negative Control #1
Pre-miR™ Negative Control #1 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. RNA oligonucleotides are susceptible to degradation by exogenous ribonucleases introduced during handling. Wear gloves when handling this product. Use RNase-free reagents, tubes, and barrier pipette tips. Upon receipt, store in a non-frost-free freezer at or below -20°C (dried oligonucleotides are shipped at ambient temperature).
NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
Stealth RNAi™ siRNA Negative Control Lo GC Duplex #2 Invitrogen™
Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are:
• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA
Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.
• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA
Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.
mirVana™ miRNA Mimic, Negative Control #1 Invitrogen™
miRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA functional analysis by up-regulation of miRNA activity.
We have now released the second generation of miRNA mimics, mirVana™ miRNA Mimics, which:
• Exhibit maximum and consistent effect in vitro at low concentration
• Are non toxic
• Offer superior specificity due to unique Star strand modification
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering you consistency throughout your entire research project
Positive and negative controls are also available for use along with mirVana™ miRNA Mimics in miRNA up-regulation experiments.
mirVana™ miRNA Mimic Negative Control #1
mirVana™ miRNA Mimic Negative Control #1 is designed to use as a negative control for experiments using mirVana™ miRNA Mimics. Transfect the Negative Control using the same methodology as for your positive control (such as miR-1 Positive Control) and experimental mirVana™ miRNA Mimics. Use target gene expression from Negative Control-transfected samples as a baseline for evaluation of the effect of the control and experimental miRNA mimic on target gene expression.
mirVana™ miRNA Mimic Negative Control #1 is a random sequence miRNA mimic molecule that has been extensively tested in human cell lines and tissues and validated to not produce identifiable effects on known miRNA function.
First Generation Mimic Controls
Our first-generation miRNA mimics (and controls), Ambion® Pre-miR™ miRNA Precursors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Mimics. These first-generation mimics are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Mimics and their corresponding controls.
We have now released the second generation of miRNA mimics, mirVana™ miRNA Mimics, which:
• Exhibit maximum and consistent effect in vitro at low concentration
• Are non toxic
• Offer superior specificity due to unique Star strand modification
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering you consistency throughout your entire research project
Positive and negative controls are also available for use along with mirVana™ miRNA Mimics in miRNA up-regulation experiments.
mirVana™ miRNA Mimic Negative Control #1
mirVana™ miRNA Mimic Negative Control #1 is designed to use as a negative control for experiments using mirVana™ miRNA Mimics. Transfect the Negative Control using the same methodology as for your positive control (such as miR-1 Positive Control) and experimental mirVana™ miRNA Mimics. Use target gene expression from Negative Control-transfected samples as a baseline for evaluation of the effect of the control and experimental miRNA mimic on target gene expression.
mirVana™ miRNA Mimic Negative Control #1 is a random sequence miRNA mimic molecule that has been extensively tested in human cell lines and tissues and validated to not produce identifiable effects on known miRNA function.
First Generation Mimic Controls
Our first-generation miRNA mimics (and controls), Ambion® Pre-miR™ miRNA Precursors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Mimics. These first-generation mimics are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Mimics and their corresponding controls.
Stealth RNAi™ siRNA Negative Control Med GC Duplex #3 Invitrogen™
Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are:
• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA
Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.
• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA
Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.
FAM™ Dye-Labeled Anti-miR™ Negative Control #1 Invitrogen™
A FAM™ dye–labeled synthetic miRNA inhibitor for miRNA functional analysis. 5 nmol is supplied, purified and ready to transfect. Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. FAM™ Dye Labeled Anti-miR™ Negative Control #1 is a random sequence Anti-miR molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is used for monitoring transfection efficiency in transfection experiments using Anti-miR miRNA Inhibitors. The fluorescent label enables direct observation of cellular uptake, distribution, and localization of the control. The molecule is labeled at its 5' end and has the same oligonucleotide sequence as unlabeled Anti-miR™ miRNA Inhibitor Negative Control #1 (SKU #AM17010).
Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. Cy™3 dye-labeled Anti-miR™ Negative Controls (SKU #AM17011) are available. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.
Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. Cy™3 dye-labeled Anti-miR™ Negative Controls (SKU #AM17011) are available. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.
Silencer™ Negative Control No. 2 siRNA Invitrogen™
Ambion® Silencer® Negative Control #2 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 40 nmol is provided.
• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use
Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.
Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use
Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.
Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
Ambion™ In Vivo Factor VII Positive Control siRNA Invitrogen™
Ambion® In Vivo siRNA Controls
• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability
• For use in mouse cells
What are Ambion® In Vivo siRNAs?
Ambion® In Vivo siRNAs are designed using the proven Silencer® Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion® In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion® In Vivo siRNAs exhibit equivalent or better potency than Silencer® Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.
Factor VII (FVII) Positive Control siRNA
Ambion® In Vivo Factor VII siRNA, In Vivo Ready, provides a positive control for experiments involving Ambion® In Vivo siRNA delivery to mice. Factor VII (FVII⁄F7; also known as proconvertin) is a vitamin K-dependent serine protease that functions as a central protein in the coagulation cascade. FVII is synthesized in the liver and secreted to the plasma where it circulates in an inactive form. After delivery of FVII-targeting siRNA to the liver, knockdown can be measured by quantifying FVII protein levels in blood by robust, commercially available chromogenic assays. Moreover, blood samples can be collected from the same animal at multiple time points post-siRNA injection. These features enable much more rapid and efficient knockdown measurements for FVII than for gene targets that require quantitation of mRNA levels in liver tissue, making FVII an ideal control for in vivo siRNA delivery.
Quality Control
Ambion® synthesizes and purifies each Ambion® In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
In Vivo Ready Controls
Our "In Vivo Ready" Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.
• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability
• For use in mouse cells
What are Ambion® In Vivo siRNAs?
Ambion® In Vivo siRNAs are designed using the proven Silencer® Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion® In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion® In Vivo siRNAs exhibit equivalent or better potency than Silencer® Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.
Factor VII (FVII) Positive Control siRNA
Ambion® In Vivo Factor VII siRNA, In Vivo Ready, provides a positive control for experiments involving Ambion® In Vivo siRNA delivery to mice. Factor VII (FVII⁄F7; also known as proconvertin) is a vitamin K-dependent serine protease that functions as a central protein in the coagulation cascade. FVII is synthesized in the liver and secreted to the plasma where it circulates in an inactive form. After delivery of FVII-targeting siRNA to the liver, knockdown can be measured by quantifying FVII protein levels in blood by robust, commercially available chromogenic assays. Moreover, blood samples can be collected from the same animal at multiple time points post-siRNA injection. These features enable much more rapid and efficient knockdown measurements for FVII than for gene targets that require quantitation of mRNA levels in liver tissue, making FVII an ideal control for in vivo siRNA delivery.
Quality Control
Ambion® synthesizes and purifies each Ambion® In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
In Vivo Ready Controls
Our "In Vivo Ready" Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.
Silencer™ Select GAPDH Positive Control siRNA Invitrogen™
Ambion® Silencer® SelectGAPDH Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided.
• Premium-quality siRNA, highly purified and ready to use
• Functionally tested in several common cell lines
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells
What are Silencer® Select siRNAs?
Silencer® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Silencer® Select GAPDH Positive Control siRNA:
The Silencer® SelectGAPDH positive control is extensively validated and an ideal "test" siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. Because it targets GAPDH mRNA (a common internal control), many assays are available to measure its effects, including real-time RT-PCR as well as the Ambion® KDalert™ GAPDH Assay Kit.
Quality Control:
Silencer® Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.
Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer® Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer® Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion® Silencer® siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
• Premium-quality siRNA, highly purified and ready to use
• Functionally tested in several common cell lines
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells
What are Silencer® Select siRNAs?
Silencer® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Silencer® Select GAPDH Positive Control siRNA:
The Silencer® SelectGAPDH positive control is extensively validated and an ideal "test" siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. Because it targets GAPDH mRNA (a common internal control), many assays are available to measure its effects, including real-time RT-PCR as well as the Ambion® KDalert™ GAPDH Assay Kit.
Quality Control:
Silencer® Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.
Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer® Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer® Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion® Silencer® siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
Stealth RNAi™ siRNA Negative Control Hi GC Invitrogen™
Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are: –Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA –Not homologous to anything in vertebrate transcriptome –Tested not to induce stress response Stealth RNAi™ siRNA Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.
Silencer™ GAPDH siRNA (human, mouse, rat) Invitrogen™
Ambion® Silencer® GAPDH Positive Control siRNA (human, mouse, rat) is ideal for developing and optimizing siRNA experiments. The control is validated for use in human, mouse, and rat cell lines. 5 tubes of 40 nmol each are provided.
• Validated siRNA controls for optimizing siRNA experiments
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
• Validated siRNA controls for optimizing siRNA experiments
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
