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CaptureSelect™ FcXP EvolveD™ Column Thermo Scientific™

CaptureSelect FcXP EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect FcXP Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect FcXP Affinity Matrix can be utilized for the purification of IgG and Fc fusion proteins directly from complex source materials and enables a robust and efficient purification process with excellent purity obtained in one step.

Features of this affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Non-animal derived

View other biosimilars and recombinant proteins affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The FcXP ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect FcXP affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: 40–50 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

One Shot™ INV110 Chemically Competent E. coli Invitrogen™

INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA

CaptureSelect™ FcXL Ligand Leakage ELISA Kit Thermo Scientific™

The CaptureSelect™ FcXL Ligand Leakage ELISA detects affinity ligand contamination of antibodies or antibody fragments purified using CaptureSelect™ FcXL Affinity Matrix. The ligand, a 13 kD recombinant protein, is coupled by multipoint attachment through stable amide bonds to give high chemical stability and low leakage. Even when covalently attached, small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This sensitive ELISA detects affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect™ FcXL Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells Invitrogen™

MultiShot FlexPlate OmniMAX 2 T1R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Abundance of colonies with OmniMAX 2 T1R cloning capabilities
MultiShot FlexPlate OmniMAX 2 T1R cells are an improved chemically competent E. coli cell line, perfect for use in all cloning applications, including Gateway cloning, and provide transformation efficiencies in the MultiShot FlexPlate format of >5 x 108 cfu/µg with control plasmid DNA. These cells also provide efficient transformation of highly methylated DNA, since OmniMAX 2 T1R cells lack the E. coli K-12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)).

Key features of the MultiShot FlexPlate OmniMAX 2 T1R Competent Cells include:
Δ(ccdAB) for sensitivity to the toxic effects of the ccdB gene product, allowing negative selection of vectors containing the ccdB gene—ideal for transformation of Gateway and TOPO reactions
mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
tonA (fhuA) genotype to confer resistance to T1 and T5 phage
Highest transformation efficiency in FlexWell format (>5x108 cfu/µg control DNA)
endA1 for increased plasmid yield and quantity
recA1 for reduced occurrence of non-specific recombination in cloned DNA
lacZΔM15 for blue/white color screening of recombinant clones

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F´ {proAB lacIq lacZΔM15 Tn10(TetR ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

Zeba™ Desalting Chromatography Cartridges, 7K MWCO, 5 mL Thermo Scientific™

Thermo Scientific Pierce Zeba Desalting Chromatography Cartridges, 7K MWCO, 2 mL contains Zeba™ High-Performance Resin for desalting or buffer exchange applications. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin and other small labeling reagents. Protein samples can be processed with unsurpassed protein recovery and ≥95% retention of salts and other small molecular contaminants (< 1,000 Da).

Features of Zeba Desalting Chromatography Cartridges, 7K MWCO, 2 mL:

High recovery—low-binding resin maximizes protein recovery
Fast—no fraction screening or waiting for protein to emerge by gravity-flow
Easy-to-use—no cumbersome column preparation or equilibration
Flexible—available in spin columns, filter spin plates and cartridges for a range of needs
Economical—great performance at lower cost than other commercially available cartridges

Zeba Desalting Cartridge-fittings are convenient, ready-to-use devices compatible with the major automated liquid-chromatography systems or for manual syringe processing. The cartridges attach directly to ÄKTA™ or FPLC Systems without additional connectors. An accessory pack included with each product readily adapts cartridges for use with Luer Syringe Fittings or 1/16” tubing. Pierce Desalting Cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses and efficiently process samples from 50 to 1500 µL.

Available Formats:
Spin Columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
Chromatography Cartridges—compatible with automated liquid chromatography systems and with syringe processing (learn more about our Chromatography Cartridges)
96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.

More Product Data
Benchmarking experiments with Zeba Spin Desalting Columns
Chromatography cartridges for desalting and affinity purification

Related Products
Zeba™ Desalting Chromatography Cartridges, 7K MWCO, 5 mL
Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL
Zeba™ Spin Desalting Columns, 7K MWCO, 2 mL
Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL
Zeba™ Spin Desalting Columns, 7K MWCO, 10 mL

One Shot™ Stbl3™ Chemically Competent E. coli Invitrogen™

One Shot® Stbl3™ Chemically Competent E. coli are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower™ Lentiviral Expression Vectors and other retroviral vectors. The DNA yield from Stbl3™ cells is often >10-fold higher than alternative strains such as TOP10 (see table). One Shot® Stbl3™ Chemically Competent Cells deliver:

• Optimal performance for the propagation of lentiviral vectors
• Enhanced genomic cloning capacity (mcrB, mrr)
• Expected efficiency of >1 × 108 transformants/µg plasmid DNA
• High DNA yields

Recommended use
The Stbl3™ E. coli strain is derived from the HB101 E. coli strain and is recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., Invitrogen® ViraPower™ Lentiviral Expression kits). The transformation efficiency of One Shot® Stbl3™ chemically competent cells is greater than 1 × 108 cfu/µg plasmid DNA.

Genotype: F-mcrB mrrhsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ-leumtl-1

Find the strain and format that you need
We offer a variety of competent cells for the propagation of unstable DNA inserts.

MAX Efficiency® Stbl2™ Competent Cells—stable cloning of direct repeat and retroviral sequences and tandem array genes (5 × 200 µL) (>1 × 109 cfu/µg plasmid DNA)
One Shot® Stbl3™ Chemically Competent E. coli—cloning unstable inserts containing direct repeats (20 × 50 µL) (>1 × 108 cfu/µg plasmid DNA)
ElectroMAX™ Stbl4™ Competent Cells—high transformation efficiencies (>5 × 109 cfu/µg plasmid DNA), ideal for generating cDNA and genomic libraries and for cloning unstable inserts

Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System Invitrogen™

The Luc-Screen® assay system with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the Luc-Screen® assay from 50 fg to 100 ng of pure enzyme in culture medium.

• Homogeneous assay format allows detection of firefly luciferase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Wide dynamic range of firefly luciferase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for b-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
Luciferase is an ideal reporter because of the high sensitivity of detection and the absence of endogenous luciferase activity in mammalian cells. The Luc-Screen® assay conveniently couples in-well cell lysis in the presence of culture medium with a high sensitivity assay that exhibits extended-glow light emission kinetics. Light signal can be measured between 10 minutes and several hours after adding assay reagents, providing great flexibility in the time between reagent addition and measurement.

High Throughput
The Luc-Screen® system is designed for maximum assay flexibility in a high throughput format and can be used in luminometers without automatic injectors. The Luc-Screen® system is formulated to provide a convenient, easy-to-use luciferase assay that is optimized for use in high-throughput screening. The high sensitivity of the Luc-Screen® assay is accompanied by a wide dynamic range.

For Research Use Only. Not for use in diagnostics procedures.

pT7CFE1-NHis-GST Vector for Mammalian Cell-Free Protein Expression Thermo Scientific™

Thermo Scientific pT7CFE1-NHis-GST is a cloning plasmid optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins. pT7CFE1-NHis-GST Vector is available with tandem affinity tags, 9xHis and GST at the N-terminus, to facilitate protein purification and detection. pT7CFE1-NHis-GST also has a cleavable tag, HRV 3C, available on the N-terminus.

Features of pT7CFE1:
• EMCV IRES at the 5' UTR promotes high-level translation of mRNAs
• MCS accommodates gene insertion via ten different restriction sites: Msc1, Nde1, BamH1, EcoR1, EcoRV, Pac1, Pst1, Sac1, Sal1, Not1 and Xho1
• Poly A sequence in the 3' region promotes mRNA stabilization and protection from nucleases
• T7 terminator ensures synthesis of accurate size mRNA transcripts
• Plasmid linearization may be accomplished with restriction sites between Poly A sequence and the T7 terminator region

pT7CFE1 Expression Vectors contain the Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) element that is critical for high levels of cap-independent protein expression in the Human In Vitro Translation System. Each vector features a highly-compatible multiple cloning site (MCS) to facilitate easy insertion of protein coding sequences into and between vectors. The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE Vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation. Custom cloning services are also available.

More Product Data
Choosing a vector and purification method for in vitro protein expression

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pT7CFE1-NHA-CHis Vector for Mammalian Cell-Free Protein Expression
pT7CFE1-CGST-HA-His Vector for Mammalian Cell-Free Protein Expression
pT7CFE1-CGFP-HA-His Vector for Mammalian Cell-Free Protein Expression
pT7CFE1-NHis-GST-CHA Vector for Mammalian Cell-Free Protein Expression

Streptavidin, (APC-Alexa Fluor™ 750) Invitrogen™

Class: RUO
Statement: For research use only. Not for use in diagnostic procedures.
Buffer: PBS
Preservative: 0.1% Sodium Azide

View a selection guide for all streptavidin RPE, APC, and tandem conjugates.

Concanavalin A, Tetramethylrhodamine Conjugate Invitrogen™

Concanavalin A (Con A) is one of the most widely used lectins in cell biology. Our tetramethylrhodamine conjugate of Con A exhibits the bright, orange-red fluorescence of tetramethylrhodamine (absorption/emission maxima ~555/580 nm). Tetramethylrhodamine Con A selectively binds to α-mannopyranosyl and α-glucopyranosyl residues.

View complete list of fluorescent dye-conjugated lectins ›

Pierce™ Protein A/G Plus UltraLink™ Resin Thermo Scientific™

Thermo Scientific Pierce Protein A/G Plus UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein A/G has been immobilized at high density for use in IgG purification methods.

Features of Protein A/G Plus UltraLink Resin:

Immobilized Protein A/G – combines the IgG binding domain of both Protein A and Protein G, enabling purification of numerous IgG subclasses and species
High capacity – this “Plus” variety has a dense load of immobilized Protein A/G, providing a binding capacity of at least 28 mg human IgG per milliliter of resin
UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

The Protein A/G Plus UltraLink Resin is used for high-capacity binding and purification of IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A/G is a recombinant fusion protein consisting of the antibody-binding domains of Protein A and Protein G, enabling antibody purification from serum samples of a variety of species.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Properties of Thermo Scientific UltraLink Resin:
• Support pH Stability: 1 to 13
• Average Particle Size: 50 to 80 microns
• Exclusion Limit: greater than 2,000,000 daltons
• Average Surface Area: greater than 250m2 per gram of beads
• Average Pore Volume: greater than 1.2 mL per gram of beads (> 60% of bead volume)
• Pore Size: 500 to 1000 angstroms (50 to 100nm)
• Maximum Linear Velocity: 3000 cm per hour
• Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Related Products
Pierce™ Protein A/G UltraLink™ Resin
Pierce™ Protein A/G Plus Agarose

Control BACULOSOMES™ Plus Reagent

Control BACULOSOMES® Plus Reagent is a microsomal preparation from insect cells infected with wild-type baculovirus. This product is suitable as a negative control for assays carried out using our CYP450 BACULOSOMES® Plus Reagents.

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

NAb™ Protein A Plus Spin Kit, 0.2 mL Thermo Scientific™

The Thermo Scientific NAb Protein A Plus Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of 100-1000µg of IgG from 25-500µL of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used. Also included in each kit are sufficient collection tubes, buffers and a streamlined protocol for purifying at least 10 antibody samples.

Pierce Protein A Plus Agarose is a versatile, high-performance affinity resin for antibody purification. It consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available varieties of immobilized Protein A affinity resins, this one provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein A Plus Agarose:

Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity – this “Plus” variety of Pierce Protein A Agarose has a dense load of immobilized Protein A, providing a binding capacity greater than 34 mg human IgG/mL resin (approx. 16 to 17 mg mouse IgG/mL resin)

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).

Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.

Pierce Protein A Plus Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A through multiple rounds of antibody purification.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein A Plus Agarose
NAb™ Protein A Plus Spin Columns, 0.2 mL

MultiShot™ FlexPlate Stbl3™ Competent Cells Invitrogen™

MultiShot FlexPlate Stbl3 chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot FlexPlate Stbl3 competent E. coli cells are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower Lentiviral Expression vectors and other retroviral vectors. The DNA yield from Stbl3 cells is often >10-fold higher than alternative strains such as TOP10 (see table).

Reduce unwanted recombination with Stbl3 cells
MultiShot FlexPlate Stbl3 Competent Cells are derived from the HB101 E. coli strain and are recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., ViraPower Lentiviral Expression kits). The transformation efficiency of these cells is greater than 1 × 107 cfu/µg plasmid DNA.

Key features of the MultiShot FlexPlate Stbl3 Competent Cells include:
Optimal performance for the propagation of lentiviral vectors
mcrB, mrr for enhanced genomic cloning capacity
recA13 for reduced occurrence of non-specific recombination in cloned DNA
High DNA yields

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F-mcrB mrr hsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ- leu mtl-1

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

Texas Red™-X Protein Labeling Kit Invitrogen™

Molecular Probes® Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor® dye colors, biotin, the hapten Oregon Green® 488, fluorescein EX, and Texas Red® dye. Each kit provides the components needed to perform three protein conjugations and purifications.

Important Features of Protein Labeling Kits:

• Labeled proteins typically ready to use in 2 hr (~30 min hands-on time)
• Designed to label 1 mg of IgG
• Simple protocol—react, separate, use
• Stabilizing proteins must be removed from the sample before labeling


The Benefits of Alexa Fluor® Dyes
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum—Section 1.3.

Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes® antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes® Handbook.

We’ll Make a Custom Antibody Conjugate for You
If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
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