Shop All Protein Analysis Reagents

Cross Reactivity


Immunoassay Kit Format


Immunoassay Kit Grade


No. of Samples


Target Organism Class


Target Specificity


eBioscience™ Streptavidin PE-eFluor™ 610 Conjugate Invitrogen™

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, immunohistochemistry, and immunocytochemistry applications. Streptavidin binds to biotin with high affinity.

Laser
Blue Laser, Green Laser, Yellow-Green Laser

Emit
607 nm

Excite
488 - 561 nm

Reported Application
Flow Cytometric Analysis

Column Extenders for Pierce™ Centrifuge Columns Thermo Scientific™

Thermo Scientific Pierce Centrifuge Column Extenders are made of polypropylene, and are designed for use in gravity-flow procedures. They fit 2 mL, 5 mL and 10 mL Pierce Centrifuge Columns.

Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL

Lab Vision™ UltraVision™ LP Detection System: HRP Polymer/AEC Chromogen Thermo Scientific™

Employ a senstive detection system based on biotin-free, polymer technology with the Thermo Scientific™ Lab Vision™ UltraVision™ LP Detection System: HRP Polymer/AEC Chromogen.

Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer/AEC Chromogen Thermo Scientific™

Take advantage of a one-step polymer system providing increased sensitivity, time savings and detection simplicity with the Thermo Scientific™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer/AEC Chromogen.

eBioscience™ Mouse Hematopoietic Lineage Biotin Panel Invitrogen™

The eBioscience Mouse Hematopoietic Lineage Flow Panel contains 5 biotinylated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their cell surface antigen expression.

Reactivity/Species
Mouse

Reported Application
Flow Cytometric Analysis

Pierce™ Microcentrifuge Tubes, 1.5 mL Thermo Scientific™

Thermo Scientific Pierce 1.5 mL Capacity Microcentrifuge Tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.

Features of 1.5 mL Capacity Microcentrifuge Tubes:

• 1.5 mL capacity
• Polypropylene formulation
• Attached snap caps

Simply add the affinity resin and sample to one of the microcentrifuge tubes, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Microcentrifuge tubes allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Related Products
Pierce™ Microcentrifuge Tubes, 2.0 mL

Lab Vision™ UltraVision™ LP Detection System: HRP Polymer/DAB Plus Chromogen Thermo Scientific™

Take advantage of a senstive detection system based on biotin-free, polymer technology with the Thermo Scientific™ Lab Vision™ UltraVision™ LP Detection System: HRP Polymer/DAB Plus Chromogen.

Active Rac1 Pull-Down and Detection Kit Thermo Scientific™

The Thermo Scientific Active Rac1 Pull-Down and Detection Kit is a complete kit for the selective enrichment and detection of GTP-bound Rac1 GTPase through specific protein interaction with the Pak1 protein-binding domain.

The Active Rac1 Pull-Down and Detection Kit includes purified GST-Pak1 protein-binding domain (PBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/wash buffer, anti-Rac1 primary antibody, SDS sample buffer, spin columns and collection tubes. The kit was validated using lysates from NIH3T3 cells, a cell line that is known to have robust Rac1 activity.

Features of the Active Rac1 Pull-Down and Detection Kit:

Highly sensitive and accurate—optimized reagents, specific anti-Rac1 antibody and Western blot procedure ensure accurate controls and semi-quantitative results
Validated—functionally tested for Rac1 detection to ensure quality and performance
Compatible—effective with a variety of cell types from mouse, rat and human sources

Applications:
• Follow activation of Rac1 GTPase during cell differentiation, migration, division and cytoskeletal rearrangement
• Study Rac1 dependent lamellipodia formation
• Study the role of active Rac1 in cancer and angiogenesis
• Monitor Rac1 activity after stimulation with growth factors
• Screen small molecule inhibitors for their effect on Rac1 activity

The Active Rac1 Pull-Down and Detection Kit was validated for function and specificity of the active Rac1 enrichment method using cell lysates treated with GTPγS to activate endogenous Rac1 and compared to lysates treated with GDP to inactivate the small GTPase. GTPγS treatment traps Rac1 in the GTP-bound, active form, resulting in a strong signal when endogenous Rac1 is present. GDP treatment pushes Rac1 into the GDP-bound, inactive state, resulting in minimal or no signal, regardless of Rac1 protein levels. The kit is optimized for Western blot detection with an HRP-conjugated secondary antibody (Goat Anti-mouse IgG, Part No. 31430) and Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080). The kit contains sufficient components for 30 pull-down assays.

Rac1 Background:
Rho family GTPases serve many cellular functions, including cell signaling, transcriptional regulation and organization of the actin cytoskeleton. This family of GTPases comprise Rho (RhoA, RhoB, and RhoC), Rac (Rac1, Rac2, Rac3, and RhoG), Cdc42 (Cdc42 and G25K), Rnd (Rnd1, Rnd2, and RhoE/Rnd3), RhoBTB family and the Miro family. These GTPases enable signal transduction from the plasma membrane to the cytosol through GPCR, tyrosine kinase, cytokine and adhesion receptors. Attachment to the plasma membrane is accomplished through geranylgeranyl lipid modifications at the carboxy-terminus of the protein. Rac1 activation results in actin polymerization and appears as membrane ruffling at the cellular periphery. Rac1 activation also results in lamelipodia formation.

The Rac1 GTPase transduces signals through tyrosine kinases, adhesion molecules or cytokine/chemokine receptors after stimulation with growth factors (EGF, insulin, PDGF, NGF), integrins (fibronectin) or chemoattractants (fMLP). For example, stimulation with EGF results in PI3 kinase activation, resulting in cell growth and reorganization at the cell periphery (membrane ruffling). Alternatively, tyrosine receptor kinase signaling through Rac1 leads to activation of the MAPK stress response pathways SAPK (JNK) and p38. Stimulation of cells with fibronectin results in integrin-mediated cell spreading. Two of the main effector proteins of Rac1 are Pak1 and phosphoinositol 4-phosphate 5-kinase. Pak1 (p65 Pak) is a kinase that activates the JNK pathway, while phosphoinositol 4-phosphate 5-kinase promotes actin filament assembly. Rac is critical for T-cell development and for promoting differentiating cells. However, the Rho family of GTPases can work agonistically during cell signaling and and antagonistically during differentiation.

More Product Data
Measure activation of small GTPases via their specific downstream effectors
Detection and localization of active GTPases in neuronal cell differentiation

Pierce™ Microcentrifuge Tubes, 2.0 mL Thermo Scientific™

Thermo Scientific Pierce 2 mL Capacity Microcentrifuge Tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.

Features of 2 mL Capacity Microcentrifuge Tubes:

• 1.5 mL capacity
• Polypropylene formulation
• Attached snap caps

Simply add the affinity resin and sample to one of the microcentrifuge tubes, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Microcentrifuge tubes allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

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Pierce™ Microcentrifuge Tubes, 1.5 mL

UltraVision™ Quanto Detection System HRP DAB Thermo Scientific™

Deliver higher lab profitability and better staining by selecting the best antibody for each test with the Thermo Scientific™ UltraVision™ Quanto HRP DAB.

Pierce™ NHS-Activated Agarose Slurry Thermo Scientific™

Thermo Scientific Pierce NHS-Activated Agarose Slurry is a high-quality, amine-reactive, beaded-agarose resin for rapid and stable immobilization of proteins, peptides and other ligands via primary amines.

Features of NHS-Activated Agarose:

Easy to use—immobilize in a simple one-step reaction with minimal hands-on time
Rapid and efficient—greater than 85% coupling for most proteins within 30 minutes
Innovative format—the dry agarose concentrates the sample, making it ideal for immobilizing dilute proteins
Safe—No hazardous chemicals needed (e.g., sodium cyanoborohydride, cyanogen bromide)
Versatile—affinity resin is adaptable to column and batch affinity chromatography techniques and FPLC applications
Compatible—use with any primary amine-containing compound
Reusable—the leak-resistant chemistry means you can reuse the affinity resin
High binding capacity—coupling capacity of >30 mg/mL

NHS-activated agarose is crosslinked, 6% beaded agarose resin that contains N-hydroxysuccinimide (NHS) functional groups. The activated resin reacts with primary amines to form stable amide linkages that covalently immobilize antibodies or other proteins for use in affinity purification procedures. Pierce NHS-Activated Agarose has a coupling capacity greater than 30 mg/mL for the slurry.

Applications:
• Rapid Immobilization of goat anti-mouse and anti-rabbit antibodies in order to purify IgG produced in animals or hybridomas.
• Bulk immobilization of Protein A for purification of monoclonal antibodies
• Immobilization of ligands for purification of recombinant proteins

Pierce NHS-Activated Agarose resin uses reliable NHS-ester Chemistry and does not require hazardous chemicals for immobilization. Other amine-reactive supports, such as periodate-oxidized resins, use toxic sodium cyanoborohydride to stabilize the reaction linkage to primary amines and take 4 to 6 hours to complete. Traditional methods such as cyanogen bromide-activated supports also couple amines; however, this chemistry results in nonspecific binding and constant slow leakage of the coupled ligand. Reactions with Pierce NHS-Activated Agarose are complete in less than one hour and yield much more stable linkages.

The NHS-Activated Agarose coupling reaction is performed in an amine-free buffer at pH 7-9. Protein coupling efficiency is typically greater than 80%, regardless of the ligand's molecular weight or pI. Once a ligand is immobilized, the prepared resin can be used for multiple affinity purification procedures. The crosslinked beaded agarose has fast linear flow potential, making it useful for gravity-flow and low- to medium-pressure applications.

Related Products
Pierce™ NHS-Activated Agarose, Dry
Pierce™ NHS-Activated Agarose Spin Columns, 2 mL
Pierce™ NHS-Activated Agarose Spin Columns, 0.2 mL

Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer/DAB Plus Chromogen Thermo Scientific™

Employ a one-step polymer system providing increased sensitivity, time savings and detection simplicity with the Thermo Scientific™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer/DAB Plus Chromogen.

Slide-A-Lyzer™ Cassette Syringes, 5 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassette Syringes are designed for efficiently loading samples into Slide-A-Lyzer cassettes and removing the sample after dialysis. The 5 mL syringes are a convenient size for use with 3 mL cassettes (66380, 66381)

Features of Slide-A-Lyzer Dialysis Syringes:

• Matched to the Slide-A-Lyzer to minimize losses
• Chemically inert - will not contaminate product
• Appropriately sized for different sample volumes

These accessories are for use with Slide-A-Lyzer Dialysis Cassettes to remove low molecular weight contaminants and perform buffer exchange, desalting and concentration.

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL

SPR, Wave Nickel Plated 1.80 OD Applied Biosystems™

This is a Procise Protein Sequencer Spare Part.

For Research Use Only. Not for use in diagnostics procedures.

Pacific Blue™ Antibody Labeling Kit Invitrogen™

Molecular Probes™ Antibody Labeling Kits provide a convenient means to label small amounts of antibodies with the violet light-excitable Pacific Orange™ or Pacific Blue™ dyes. This kit is optimized for labeling 100 µg of antibody per reaction. Comparably small amounts of other proteins (>40 kDa) can also be labeled.

View a selection guide for all Antibody Labeling Kits.

View the Fluorophore Selection Guide.

The kit contains everything you need to perform five separate labeling reactions as well as to purify the resulting conjugates. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, indirect FISH, and more.

Important features of antibody labeling kits:
• Pacific Blue™ has an excitation and emission maximum of 410/455 nm
• Labeled proteins typically ready to use in 90 min (~15 min hands-on time)
• Useful for labeling 100 µg of protein
• Optimized for small-scale labeling of any protein >40 kDa
• Purified using convenient spin filters
• Stabilizing proteins must be removed from the sample before labeling
• Includes detailed instructions for determining degree of labeling (DOL)


Better results and workflows with primary labeled antibodies
A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Further, multiple primary antibodies of the same isotype or derived from the same species can easily be used in the same experiment if they are directly labeled with compatible fluorophores.

Learn more about protein and antibody labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes™ Handbook.

We'll make a custom antibody conjugate for you
If you can't find what you're looking for in our stocked list, we'll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.
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