Shop All Special Purpose IgG Purification Resins and Kits

Melon™ Gel Chromatography Cartridges, 1 mL Thermo Scientific™

The Thermo Scientific Melon Gel Chromatography Cartridge is a convenient, ready-to-use prepacked chromatography column for the isolation and purification of antibodies. Our Melon Gel IgG Purification Resin offers a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Features of Melon Gel IgG Purification Resin:

Simple, one-step protocol—IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
Rapid purification—antibody purification from serum is four to six times faster than Protein A or G methods
High recovery and purity—for many species of serum antibody recovery is greater than 90% with purity greater than 80%
Minimal dilution—reduced need to concentrate antibody before storage or use
Gentle purification—No harsh antibody elution conditions helps retain IgG activity
Regenerable support—Melon Gel Resin can be used for multiple antibody purifications

These cartridges are compatible with major automated liquid-chromatography systems or manual syringe processing, and attach directly to ÄKTA™ or FPLC Systems without additional connectors. An accessory pack, included with each product, readily adapts cartridges for use with Luer-Lok Syringe Fittings or 1/16” tubing. The Melon Gel Cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.

Melon Gel IgG Purification Resin enables purification of antibodies from serum and other sample types. Melon Gel Resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction. The procedure is gentle because no harsh elution conditions are involved.

Melon Gel Resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because Melon Gel Resin is not a bind-and-release support, it is extremely fast and gentle to ant ibodies, resulting in antibody preparations of high purity and high activity.

Melon Gel Resin works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with Melon Gel Resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Related Products
Melon™ Gel Purification Buffer
Melon™ Gel Regenerant

Melon™ Gel IgG Spin Purification Kit Thermo Scientific™

The Thermo Scientific Melon Gel IgG Spin Purification Kit offers a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Features of Melon Gel IgG Purification Kits:

Simple, one-step protocol—IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
Rapid purification—antibody purification from serum is four to six times faster than Protein A or G methods
High recovery and purity—for many species of serum antibody recovery is greater than 90% with purity greater than 80%
Minimal dilution—reduced need to concentrate antibody before storage or use
Robust purification—Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G
Gentle purification—No harsh antibody elution conditions helps retain IgG activity
Regenerable support—Melon Gel Resin can be used for multiple antibody purifications

Melon Gel IgG Purification Kits enable purification of antibodies from serum and other sample types. Melon Gel Resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction. The procedure is gentle because no harsh elution conditions are involved.

Melon Gel Resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because Melon Gel Resin is not a bind-and-release support, it is extremely fast and gentle to antibodies, resulting in antibody preparations of high purity and high activity.

Melon Gel Resin works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with Melon Gel Resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Related Products
Melon™ Gel IgG Purification Kit
Melon™ Gel Purification Buffer
Melon™ Gel Regenerant

Melon™ Gel Monoclonal IgG Purification Kit Thermo Scientific™

The Thermo Scientific Melon Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for monoclonal antibodies from ascites or hybridoma cell culture supernatant, or for large scale antibody purification from serum.

Features of the Melon Gel Monoclonal IgG Purification Kit:

Fast—purify antibodies in half the time of required for Protein A or G
Efficient—recovery and purity is often better than Protein A or G purification
Robust—effective for subclasses of IgG that bind poorly to Protein A or G
Gentle—antibodies retain activity because they are not exposed to denaturing conditions
Regenerable—support can be regenerated and used for up to 6 purifications
Compatible—amine-free buffer does not require removal or neutralization
Flexible—accommodates a broad range of sample volumes and processing vessels; cell culture: spin columns can be used for purifications < 30 mL of supernatant; filter flasks can be used for bulk processing of samples up to 1L. Ascites fluid: spin columns can be used for purifications up to 6 mL

Unlike typical bind-and-elute affinity methods, Melon Gel Purification is based on the binding of non-IgG proteins from a prepared supernatant or ascites sample, thereby allowing the pure IgG to be recovered in the flow-through (non-bound) fraction. Antibodies are recovered ready for use in assays or subsequent conjugation and other modification steps. There is no need to neutralize or desalt to mitigate the effects of harsh elution buffers. Melon Gel Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody.

Specialized protocols for culture supernatant and ascites fluid:
The instructions for the Melon Gel Monoclonal IgG Purification include separate protocols for cell culture supernatant samples and ascites fluid samples. For the latter protocol, Ascites Conditioning Reagent (Part No. 45219) is required.

Other IgG purification kits and package sizes of Melon Gel Resin are available. All sizes use the same formulation of Melon Gel Resin, Purification Buffer and Regenerant Solution but have slightly different protocols based on the most common application for each package size. Components of any package size can be used at an appropriate scale with any one of the procedures.

Related Products
Ascites Conditioning Reagent for Melon™ Gel Monoclonal IgG Purification Kit

Melon™ Gel Purification Buffer Thermo Scientific™

Thermo Scientific Melon Gel Purification Buffer, formulation: proprietary buffer dry-blend, sufficient for preparing 200 mL of 100X buffer.

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Melon™ Gel IgG Spin Purification Kit
Melon™ Gel IgG Purification Kit
Melon™ Gel Regenerant

Melon™ Gel Spin Plate Kit for IgG Screening Thermo Scientific™

The Melon Gel Spin Plate Kit for IgG Screening offers a high-throughput format for quick purification and screening of antibodies. This kit enables purification of up to 96 samples of 20-100 µl in 15 minutes or less, offering a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Features of Melon Gel IgG Purification Resin:

Simple, one-step protocol—IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
Rapid purification—antibody purification from serum is four to six times faster than Protein A or G methods
High recovery and purity—for many species of serum antibody recovery is greater than 90% with purity greater than 80%
Minimal dilution—reduced need to concentrate antibody before storage or use
Gentle purification—No harsh antibody elution conditions helps retain IgG activity
Regenerable support—Melon Gel Resin can be used for multiple antibody purifications

Melon Gel IgG Purification Kits enable purification of antibodies from serum and other sample types. Melon Gel Resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction. The procedure is gentle because no harsh elution conditions are involved.

Melon Gel Resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because Melon Gel Resin is not a bind-and-release support, it is extremely fast and gentle to ant ibodies, resulting in antibody preparations of high purity and high activity.

Melon Gel Resin works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with Melon Gel Resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Related Products
Melon™ Gel IgG Spin Purification Kit
Melon™ Gel Purification Buffer
Melon™ Gel Regenerant

Pierce™ Antibody Clean-up Kit Thermo Scientific™

The Thermo Scientific Pierce Antibody Clean-Up Kit is for removing BSA, gelatin and other stabilizer proteins from antibody stock solutions before amine-based chemical labeling and conjugation procedures.

Features of the Antibody Clean-Up Kit:

Fast—removes carrier protein from antibody solutions in half the time required for purifying antibodies through Protein A or G purification procedures
Gentle no need to neutralize or desalt to mitigate the effects of harsh elution buffers
Complete—supplied desalting columns provide for easy buffer exchange into the required purification buffer or quick exchange into final buffer of choice
Economical—sufficient reagents to perform 10 antibody clean-up reactions

This is a complete and easy-to-use kit for removing BSA and gelatin stabilizing proteins (up to 1%) from IgG samples. The protocol uses Thermo Scientific Melon Gel to bind these frequently used storage proteins, allowing the antibody to flow through in a purified form. The antibody is recovered in an amine-free and carboxyl-free buffer, ready for use in downstream applications such as biotinylation, fluorescent labeling, crosslinking, immobilization and enzymatic fragmentation (e.g., Fab preparation).

Bovine serum albumin (BSA) and gelatin are often added to purified antibody solutions as stabilizers for long-term storage. Inclusion of 0.2 to 1% (2 to 10 mg/mL) BSA or gelatin helps stabilize dilute antibody solutions. Generally these added proteins do not interfere with immunodetection methods; however, their presence in solution does interfere with amine-reactive conjugation chemistries that are the basis for most biotinylation, fluorescent dye labeling, antibody fragmentation and covalent immobilization methods. For such methods, the antibody must be isolated from the stabilizing proteins, and the Antibody Clean-up Kit accomplishes this efficiently.

Related Products
Melon™ Gel IgG Spin Purification Kit

Melon™ Gel Regenerant Thermo Scientific™

Thermo Scientific Melon Gel Regenerant, formulation: proprietary powder dry-blend,sufficient for preparing 500 mL of regenerant.

Related Products
Melon™ Gel IgG Spin Purification Kit
Melon™ Gel IgG Purification Kit
Melon™ Gel Purification Buffer

Melon™ Gel IgG Purification Kit Thermo Scientific™

The Thermo Scientific Melon Gel IgG Purification Kit offers a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Features of Melon Gel IgG Purification Kits:

Simple, one-step protocol—IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
Rapid purification—antibody purification from serum is four to six times faster than Protein A or G methods
High recovery and purity—for many species of serum antibody recovery is greater than 90% with purity greater than 80%
Minimal dilution—reduced need to concentrate antibody before storage or use
Robust purification—Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G
Gentle purification—No harsh antibody elution conditions helps retain IgG activity
Regenerable support—Melon Gel Resin can be used for multiple antibody purifications

Melon Gel IgG Purification Kits enable purification of antibodies from serum and other sample types. Melon Gel Resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction. The procedure is gentle because no harsh elution conditions are involved.

Melon Gel Resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because Melon Gel Resin is not a bind-and-release support, it is extremely fast and gentle to ant ibodies, resulting in antibody preparations of high purity and high activity.

Melon Gel Resin works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with Melon Gel Resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Related Products
Melon™ Gel IgG Spin Purification Kit
Melon™ Gel Purification Buffer
Melon™ Gel Regenerant
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