Competent Cells

Competent Cells

Cell competence refers to the ability of a cell to take up extracellular DNA from the environment via transformation. These products include competent cells for subcloning, protein expression, and genetic library creation.
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Sf9 Cells in Grace's Gibco™

Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, from the parental IPLBSF-21 (Sf21) cell line, which was derived from the pupal ovarian tissue of the fall army...

High Five™ Cells in Express Five™ Medium Gibco™

High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichoplusia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System (BEVS).

DH5α Competent Cells Thermo Scientific™

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar...

Tali™ Viability Kit - Dead Cell Red Invitrogen™

The Tali® Viability Kit - Dead Cell Red is a ready-to-use solution of propidium iodide (PI) that has been validated for use with the Tali® Image-Based Cytometer. PI is a live cell-impermeant fluorogenic DNA-binding dye that has been extensively used to identify necrotic cells.

Grace's Insect Medium (2X), supplemented Gibco™

Grace's Supplemented Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21.

FreeStyle™ F17 Expression Medium Gibco™

Gibco FreeStyle F17 Expression Medium is a chemically defined, protein-free, serum-free medium developed to support the growth and transfection of HEK293 cells in suspension. The addition of 4–8 mM L-glutamine or GlutaMAX supplement is required before use.

One Shot™ ccdB Survival™ 2 T1R Competent Cells Invitrogen™

One Shot® ccdB Survival™ 2 T1R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors.

Expi293™ Met (–) Expression Medium Gibco™

Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells. The medium is part of the Expi293 platform for rapid, high-yield transient protein expression from...

MAX Efficiency™ DH5α Competent Cells Invitrogen™

MAX Efficiency DH5α Competent Cells are a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from...

Subcloning Efficiency™ DH5α Competent Cells Invitrogen™

Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any application where...

ElectroMAX™ DH10B Cells Invitrogen™

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/µg plasmid DNA. ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

FreeStyle™ 293 Expression Medium Gibco™

FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required.

MAX Efficiency™ DH10Bac Competent Cells Gibco™

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac, to create an expression bacmid...

Express Five™ SFM Gibco™

Express Five® SFM is a protein-free, serum-free medium specifically developed to support the long-term suspension growth of High Five™ (BTI-TN-5B1-4) cells in suspension for use in the Baculovirus Expression Vector System (BEVS). This medium requires the addition of 16 mM L-glutamine before use.

One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells Invitrogen™

One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain. DH5α-T1R carry the tonA genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction.

FreeStyle™ 293 Expression Medium Gibco™

FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required.

CD Hybridoma AGT™ Medium Gibco™

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas...

CD Hybridoma Medium Gibco™

CD Hybridoma Medium is an animal origin–free, chemically defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas •...

ElectroMAX™ DH5α-E Competent Cells Invitrogen™

ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. They may be used in procedures requiring high transformation efficiencies, such as generation of cDNA libraries or in transformations with limited input DNA.

Sf9 cells in Sf-900™ II SFM Gibco™

Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf9 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ II SFM, which saves significant time and expense associated with the adaptation of cultures.

Grace's Insect Medium, unsupplemented Gibco™

Grace's Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. One supplemented, Grace's Unsupplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21.

293 F Cells, in CD 293 Gibco™

Gibco® 293-F cells were cloned from the original 293 cell line and adapted to Gibco® CD 293 Medium. The 293 cell line is a permanent line established from primary embryonic human kidney and transformed with sheared human adenovirus type 5 DNA.

Grace's Insect Medium, supplemented Gibco™

Grace's Insect Medium, Supplemented, was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21.

Hybridoma-SFM, powder Gibco™

Gibco® Hybridoma SFM is a serum-free, very low-protein medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® Hybridoma SFM features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation...

Mimic™ Sf9 Insect Cells Gibco™

Mimic™ Sf9 Insect Cells are a derivative of the Sf9 insect cell line. These cells have been modified to stably express a variety of mammalian glycosyltransferases. Typically, insect cells are unable to process N-glycans to the extent that mammalian cells do.

Sf-900™ III SFM Gibco™

Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems.

Sf-900™ II SFM, no methionine, no cystine Gibco™

Sf-900™ II SFM (without methionine and cystine) is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector...

Medium NCTC-109 (1X) Gibco™

NCTC-109 medium is a chemically defined medium that was originally developed by the National Cancer Institute and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment.

Schneider's Drosophila Medium Gibco™

Schneider's Drosophila Medium was originally designed for the growth of S2 cells from the fruit fly, Drosophila melanogaser. Schneider's Drosophila Medium can also be used to culture other dipteran cell lines.

Tali™ Calibration Beads Invitrogen™

This product contains three tubes of beads which are used to properly calibrate and align the Tali® Image-Based Cytometer. Contents: • Tali® Alignment Beads, • Tali® Green Calibration Beads • Tali® Red Calibration Beads The calibration function of the Tali® Image-Based Cytometer recalculates the...
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