Competent Cells

Competent Cells

Cell competence refers to the ability of a cell to take up extracellular DNA from the environment via transformation. These products include competent cells for subcloning, protein expression, and genetic library creation.
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Cloning Methylated DNA

Detection Method

T-REx™-293 Cell Line Invitrogen™

T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ.

Flp-In™-CV-1 Cell Line Invitrogen™

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus.

One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells Invitrogen™

DH10B T1 Phage-Resistant E. colihave all the benefits of the DH10B genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to...

Sf9 cells in Sf-900™ III SFM Gibco™

Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf9 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ III SFM, which saves significant time and expense associated with the adaptation of...

Sf21 Cells in Grace's Gibco™

Gibco® Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, where they were derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda.

Tali™ Cell Cycle Kit Invitrogen™

The Tali® Cell Cycle Kit includes a ready-to-use reagent that, when used with the Tali® Image-Based Cytometer, provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

One Shot™ BL21(DE3) Chemically Competent E. coli Invitrogen™

One Shot® BL21(DE3) Chemically Competent E. coli are ideal for use with bacteriophage T7 promoter-based expression systems (e.g., pRSET, pCR™T7, and pET). BL21(DE3) cells carry the lambda DE3 lysogen. Recombinant proteins that are nontoxic to E.

One Shot™ BL21(DE3)pLysE Chemically Competent E. coli Invitrogen™

BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli, BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme.

MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells Invitrogen™

MultiShot FlexPlate OmniMAX 2 T1R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

DH5α Competent Cells for Subcloning Thermo Scientific™

Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

MultiShot™ StripWell TOP10 Chemically Competent E. coli Invitrogen™

MultiShot StripWell TOP10 chemically competent E. coli cells are cloning competent cells that packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations. TOP10 competent E.

One Shot™ TOP10/P3 Chemically Competent E. coli Invitrogen™

TOP10/P3 E. coliallow high-quality plasmid preparation of pCDM8, pcDNA™1.1, or any other supF-containing vector. The strain features: • P3 plasmid for selection of supF-containing plasmids • Mutated endA1 gene for increased quality of plasmid DNA preparations

BL21 Star™ (DE3)pLysS One Shot™ Chemically Competent E. coli Invitrogen™

One Shot™ BL21 Star™ (DE3) pLysS E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from high copy number, T7 promoter-based expression systems (e.g., pRSET T7 vectors).

BL21-AI™ One Shot™ Chemically Competent E. coli Invitrogen™

One Shot® BL21-AI™ E. coli are chemically competent cells designed for applications that require tight regulation and strong expression of toxic proteins from any T7 promoter-based expression systems. One Shot® BL21-AI™ Chemically Competent cells have a transformation efficiency of >1×108 cfu/ µg...

MAX Efficiency™ DH5αF´IQ Competent Cells Invitrogen™

MAX Efficiency DH5αF´IQ Chemically Competent Cells are high-efficiency chemically competent cells available for M13 cloning, cloning into plasmids with an f1-like origin of replication (phagemids), or cloning into any vector that uses expression from the lac promoter.
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