Competent Cells

Competent Cells

Cell competence refers to the ability of a cell to take up extracellular DNA from the environment via transformation. These products include competent cells for subcloning, protein expression, and genetic library creation.
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Cloning Methylated DNA

Detection Method

Tali™ Calibration Beads Invitrogen™

This product contains three tubes of beads which are used to properly calibrate and align the Tali® Image-Based Cytometer. Contents: • Tali® Alignment Beads, • Tali® Green Calibration Beads • Tali® Red Calibration Beads The calibration function of the Tali® Image-Based Cytometer recalculates the...

Drosophila S2 Cells in Schneider's Medium Gibco™

Gibco® Drosophila S2 cells are used for heterologous protein expression in the DrosophilaM Expression System (DES®). The S2 cell line was derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos.

MAX Efficiency™ DH10Bac Competent Cells Gibco™

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac, to create an expression bacmid...

Express Five™ SFM Gibco™

Express Five® SFM is a protein-free, serum-free medium specifically developed to support the long-term suspension growth of High Five™ (BTI-TN-5B1-4) cells in suspension for use in the Baculovirus Expression Vector System (BEVS). This medium requires the addition of 16 mM L-glutamine before use.

CD Hybridoma Medium Gibco™

CD Hybridoma Medium is an animal origin–free, chemically defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas •...

Tali™ Viability Kit - Dead Cell Red Invitrogen™

The Tali® Viability Kit - Dead Cell Red is a ready-to-use solution of propidium iodide (PI) that has been validated for use with the Tali® Image-Based Cytometer. PI is a live cell-impermeant fluorogenic DNA-binding dye that has been extensively used to identify necrotic cells.

FreeStyle™ F17 Expression Medium Gibco™

Gibco FreeStyle F17 Expression Medium is a chemically defined, protein-free, serum-free medium developed to support the growth and transfection of HEK293 cells in suspension. The addition of 4–8 mM L-glutamine or GlutaMAX supplement is required before use.

Sf9 Cells in Grace's Gibco™

Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, from the parental IPLBSF-21 (Sf21) cell line, which was derived from the pupal ovarian tissue of the fall army...

High Five™ Cells in Express Five™ Medium Gibco™

High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichoplusia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System (BEVS).

One Shot™ PIR1 Chemically Competent E. coli Invitrogen™

The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many...

One Shot™ ccdB Survival™ 2 T1R Competent Cells Invitrogen™

One Shot® ccdB Survival™ 2 T1R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors.

MultiShot™ TOP10 Chemically Competent E. coli Invitrogen™

MultiShot™ TOP10 Chemically Competent E. coli provide transformation efficiencies of >1 x 108 cfu/µg control plasmid DNA, and are suited for high-throughput transformations. MultiShot™ TOP10 Chemically Competent cells are packaged in five 96-well microtiter plates (15 µL aliquots) to simplify...

One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli Invitrogen™

One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells currently available (see figure). Doubling time is approximately 50 minutes, compared to >74 minutes for other cloning strains.

MultiShot™ StripWell DH5α T1R Competent Cells Invitrogen™

MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell DH5α T1R competent E.

One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli Invitrogen™

One Shot® BL21 Star™ (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion™ pET vectors).
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