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Sf9 Cells in Grace's Gibco™

Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, from the parental IPLBSF-21 (Sf21) cell line, which was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Gibco® Sf9 cells (frozen in Grace's Insect Medium) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Protocols for adherent or suspension growth in Gibco® Supplemented Grace's Insect Medium are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow.

Small, regular size that generates even monolayers and plaques
Gibco® Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.

Quality and performance testing
Each lot of Gibco® Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

BL21 Star™ (DE3)pLysS One Shot™ Chemically Competent E. coli Invitrogen™

One Shot® BL21 Star™ (DE3) pLysS E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from high copy number, T7 promoter-based expression systems (e.g., pRSET T7 vectors). One Shot® BL21 Star™ (DE3) pLysS Chemically Competent cells have a transformation efficiency of >1 x 108 cfu/ µg plasmid DNA.
• High mRNA stability results in increased protein yield
• Ideal for use with high copy number, T7 promoter-based plasmids
• Low background expression in uninduced cells

Increased mRNA Stability Improves Protein Yield
One Shot® BL21 Star™ (DE3) pLysS E. coli offers enhanced mRNA stability so that abundant mRNA is available for protein expression. This enhanced stability is due to a mutation in the RNaseE gene (rne131), which is involved in mRNA degradation.

High Expression of Non-Toxic Recombinant Proteins
One Shot® BL21 Star™ (DE3) pLysS cells are ideal for high-level expression of non-toxic but potentially growth-inhibiting recombinant proteins from high copy number, T7 promoter-based expression systems. The pLysS CamR plasmid carried by the BL21 Star™ (DE3) pLysS strain expresses T7 lysozyme, a T7 RNA polymerase inhibitor that prevents leaky expression in uninduced cells. The p15a origin of pLysS makes this plasmid compatible with pUC or pBR322-derived plasmids. This strain also contains the DE3 lysogen that carries the T7 RNA polymerase gene under control of the lacUV5 promoter, and IPTG is required to induce expression of the T7 RNA polymerase. One Shot® BL21 Star™ (DE3) pLysS cells do not contain the lon protease and are also deficient in the outer membrane protease, OmpT. The absence of these proteases reduces degradation of heterologous proteins.

Note: The BL21 Star™ strains have higher basal expression of heterologous genes than BL21 strains, due to the increased stability of mRNA. Therefore, these strains may not be useful for expression of toxic genes. However, the BL21 Star™ (DE3) pLysS strains offer lower expression levels than the BL21 Star™ strains.

Genotype:
F-ompT hsdSB (rB-, mB-) galdcmrne131 (DE3) pLysS (CamR)

Easy-to-use, Single Tube Format
The single tube, single use, format allows for all steps of transformation up to plating to take place in the same tube, thereby helping to save time and prevent contamination.

Find the Strain and Format That You Need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. For expression of toxic proteins, choose BL21-AI™ One Shot® Chemically Competent E. coli. For expression of non-toxic recombinant proteins from low copy number (e.g. Champion™ pET vectors), T7 promoter-based expression system, choose our One Shot® BL21 Star™ (DE3) chemically competent cells.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MultiShot™ TOP10 Chemically Competent E. coli Invitrogen™

MultiShot™ TOP10 Chemically Competent E. coli provide transformation efficiencies of >1 x 108 cfu/µg control plasmid DNA, and are suited for high-throughput transformations. MultiShot™ TOP10 Chemically Competent cells are packaged in five 96-well microtiter plates (15 µL aliquots) to simplify high-throughput bacterial transformations. Benefits of MultiShot™ TOP10 cells:

• Designed for high-throughput applications—microtiter plate format designed for automated high-throughput cloning
• Compatible with genomic DNA—the mcrA mutation supports efficient transfection of methylated DNA
• Permits rapid screening—the LacZΔM15 allele permits rapid blue/white screening of transfectants on plates containing X-Gal or Bluo-Gal
• Efficient—the endA1 mutation helps reduce endonuclease activity, improving subsequent plasmid DNA yields
• Reliable—the recA1 mutation helps reduce unwanted recombinationEasy to use for high-throughput transformations
MultiShot™ TOP10 Chemically Competent cells are genetically similar to the reliable DH10B™ strain, and are available in five 96-well plates to facilitate high-throughput transformation. After addition of DNA, MultiShot™ TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler. Using our TA Cloning® and TOPO® Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected.

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot™ formatted comp cells or contact us for custom formatting options.

High Five™ Cells in Express Five™ Medium Gibco™

High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichoplusia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System (BEVS). High Five™ Insect Cells are adapted to serum-free culture in Express Five® SFM for maximum cell growth and recombinant gene expression. High Five™ Insect Cells (frozen in Express Five® SFM) features:

• Recombinant protein expression from a variety of expression systems
• Up to ten-fold increase in secreted protein expression compared to Sf9 cells
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in High Five™ cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Up to ten-fold increase in secreted protein expression compared to Sf9 cells
High Five™ cells are the best choice for expression of secreted protein in insect cells. They consistently express 5–10 times more protein that Sf9 cells.

Good growth in adherent or suspension culture
High Five ™ cells thaw into adherent culture, forming an irregular monolayer with plaques that may be difficult to isolate. Protocols for adherent growth can be found in Express Five® SFM are available in the product manual. High Five™ cells can be adapted to suspension culture.

Quality and performance testing
Each lot of High Five ™ cells is tested for cell growth and viability post-recovery from cryopreservation.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

One Shot™ BL21(DE3)pLysS Chemically Competent E. coli Invitrogen™

BL21(DE3)pLysS cells are ideal for use with T7 promoter-based expression systems (e.g., pRSET, pCR™T7, and pET). BL21(DE3)pLysS E. coli carry both the DE3 lysogen and the plasmid pLysS. pLysS constitutively expresses low levels of T7 lysozyme, which reduces basal expression of recombinant genes by inhibiting basal levels of T7 RNA polymerase.

One Shot™ Stbl3™ Chemically Competent E. coli Invitrogen™

One Shot® Stbl3™ Chemically Competent E. coli are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower™ Lentiviral Expression Vectors and other retroviral vectors. The DNA yield from Stbl3™ cells is often >10-fold higher than alternative strains such as TOP10 (see table). One Shot® Stbl3™ Chemically Competent Cells deliver:

• Optimal performance for the propagation of lentiviral vectors
• Enhanced genomic cloning capacity (mcrB, mrr)
• Expected efficiency of >1 × 108 transformants/µg plasmid DNA
• High DNA yields

Recommended use
The Stbl3™ E. coli strain is derived from the HB101 E. coli strain and is recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., Invitrogen® ViraPower™ Lentiviral Expression kits). The transformation efficiency of One Shot® Stbl3™ chemically competent cells is greater than 1 × 108 cfu/µg plasmid DNA.

Genotype: F-mcrB mrrhsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ-leumtl-1

Find the strain and format that you need
We offer a variety of competent cells for the propagation of unstable DNA inserts.

MAX Efficiency® Stbl2™ Competent Cells—stable cloning of direct repeat and retroviral sequences and tandem array genes (5 × 200 µL) (>1 × 109 cfu/µg plasmid DNA)
One Shot® Stbl3™ Chemically Competent E. coli—cloning unstable inserts containing direct repeats (20 × 50 µL) (>1 × 108 cfu/µg plasmid DNA)
ElectroMAX™ Stbl4™ Competent Cells—high transformation efficiencies (>5 × 109 cfu/µg plasmid DNA), ideal for generating cDNA and genomic libraries and for cloning unstable inserts

One Shot™ TOP10 Chemically Competent E. coli Invitrogen™

One Shot® TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 109 cfu/µg plasmid DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits. One Shot® TOP10 cells:

• Maximize cloning efficiency in a single-tube format
• Provide enhanced genomic DNA cloning capabilities

Easy-to-use One Shot® format
The single-tube, single-use format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping to save time and to prevent contamination.

Versatile cloning capabilities
One Shot® TOP10 E. coli cells are similar to the DH10B™ strain, and offer the following features:

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Genotype: F- mcrA Δ( mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ( araleu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require high-throughput transformation, choose from our collection of MultiShot™ formatted comp cells.

Sf-900™ Medium (1.3X) Gibco™

Sf-900™ SFM is a complete serum-free, low protein insect cell culture medium developed for the convenient and reproducible formation of 1% agarose overlays used for plaque assays of baculovirus in Spodoptera frugiperda (Sf9 and Sf21) cells. When used with Gibco® 4% Agarose Gel, Sf900™ SFM (1.3x) allows for convenient formation of a 1% overlay with correct 1x final osmolality and nutrition required for insect cell growth during the plaque assay.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Sf-900 II SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

MultiShot™ FlexPlate Stbl3™ Competent Cells Invitrogen™

MultiShot FlexPlate Stbl3 chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot FlexPlate Stbl3 competent E. coli cells are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower Lentiviral Expression vectors and other retroviral vectors. The DNA yield from Stbl3 cells is often >10-fold higher than alternative strains such as TOP10 (see table).

Reduce unwanted recombination with Stbl3 cells
MultiShot FlexPlate Stbl3 Competent Cells are derived from the HB101 E. coli strain and are recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., ViraPower Lentiviral Expression kits). The transformation efficiency of these cells is greater than 1 × 107 cfu/µg plasmid DNA.

Key features of the MultiShot FlexPlate Stbl3 Competent Cells include:
Optimal performance for the propagation of lentiviral vectors
mcrB, mrr for enhanced genomic cloning capacity
recA13 for reduced occurrence of non-specific recombination in cloned DNA
High DNA yields

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F-mcrB mrr hsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ- leu mtl-1

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

MultiShot™ FlexPlate DH5α T1R Competent Cells Invitrogen™

MultiShot FlexPlate DH5α T1R chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

MultiShot FlexPlate DH5α T1R competent E. coli cells are derived from the popular DH5α strain, one of the most frequently used laboratory cloning strains. All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Reliable DH5α T1R cloning capabilities
The DH5α strain is the standard laboratory strain for chemical transformations as it is ideal for transformations of cloned unmethylated DNA. The DH5α T1R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 108 cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate DH5α T1R Competent Cells include:
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA
tonA (fhuA) for T1 and T5 phage resistance

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 gyrA96 relA1 tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

One Shot™ BL21(DE3) Chemically Competent E. coli Invitrogen™

One Shot® BL21(DE3) Chemically Competent E. coli are ideal for use with bacteriophage T7 promoter-based expression systems (e.g., pRSET, pCR™T7, and pET). BL21(DE3) cells carry the lambda DE3 lysogen. Recombinant proteins that are nontoxic to E. coli are generally expressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS or BL21(DE3)pLysE. However, the basal expression levels of heterologous genes are significantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.

About BL21 strains
BL21 strains are descended from the E. coli B strain and have been specifically constructed for high-level expression of recombinant proteins. These strains have two important attributes that make them ideal for protein expression: key genetic markers and inducibility of protein expression. The most important genetic markers help recombinant RNA and/or protein accumulate to high levels without degradation. Inducibility helps to minimize the toxic effects of some recombinant proteins.

FreeStyle™ CHO Expression Medium Gibco™

FreeStyle™ CHO Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation.

This medium requires supplementation with 8 mM glutamine before use. Gibco® FreeStyle™ CHO Expression Medium features:

• Formulation without hypoxanthine and thymidine for use with or without dhfr systems
• Ability to support high-density suspension growth and transient transfection of CHO-S cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
• Scalability in spinner flasks and bioreactors

Formulation without hypoxanthine and thymidine for use with or without dhfr systems
Gibco® FreeStyle™ CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems. Non-dhfr systems require the addition of HT Supplement before use.

Ability to support high-density suspension growth and transient transfection of CHO-S cells
Gibco® FreeStyle™ CHO cells in suspension can be cultured directly into Gibco® FreeStyle™ CHO Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ CHO Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ CHO Expression Medium can be transfected using lipid-based transfection reagents, such as Freestyle™ MAX reagent.

Animal origin–free, protein-free and chemically defined formulation
Gibco® FreeStyle™ CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
The FreeStyle™ MAX CHO Expression System is a breakthrough technology for rapid (see figure) and high-yield mammalian protein production in 30 mL and 1 L batch sizes (see figure). The FreeStyle™ MAX CHO Expression System combines GIBCO® FreeStyle™ media, FreeStyle™ MAX reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells in one kit.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ CHO Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ CHO Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco® FreeStyle™ CHO Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

One Shot™ PIR1 Chemically Competent E. coli Invitrogen™

The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA strands. The end result is a fusion of the donor and acceptor vectors forming a single, functional expression vector.
PIR1 competent cells are for cloning and maintenance of your donor vector (i.e. pUniV5/His-TOPO®) construct (or other vector containing the R6K-gamma origin). It contains a mutant allele of the pir gene that maintains the donor vector construct at ~250 copies per cell.

MC1061/P3 Chemically Competent E. coli Invitrogen™

MC1061/P3 Chemically Competent E. coli are used for highly efficient transformation of vectors that require the P3 episome for selection and maintenance (i.e., pCDM8, pcDNA™1.1, or any other supF-containing vector).

Contents
• 5 x 300 μL competent MC1061/P3 E. coli
• 50 µL pUC19 plasmid DNA (10 pg/μL in 5 mM Tris-HCl, 0.5 mM EDTA, pH 8)
• 15 mL SOC medium

Sf-900™ III SFM Gibco™

Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Features of Gibco® Sf-900™ III SFM:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, animal origin–free, ready-to-use formulation
• Improved lot-to-lot consistency

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 106 cells/mL, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see figure).

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.

Serum-free, protein-free, animal origin–free, ready-to-use formulation
Gibco® Sf-900™ III SFM is a serum-free, protein-free, animal origin–free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).

Improved lot-to-lot consistency
Gibco® Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM (see figure).

cGMP manufacturing and quality system
Gibco® Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
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