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One Shot™ INV110 Chemically Competent E. coli Invitrogen™

INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA

MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells Invitrogen™

MultiShot FlexPlate OmniMAX 2 T1R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Abundance of colonies with OmniMAX 2 T1R cloning capabilities
MultiShot FlexPlate OmniMAX 2 T1R cells are an improved chemically competent E. coli cell line, perfect for use in all cloning applications, including Gateway cloning, and provide transformation efficiencies in the MultiShot FlexPlate format of >5 x 108 cfu/µg with control plasmid DNA. These cells also provide efficient transformation of highly methylated DNA, since OmniMAX 2 T1R cells lack the E. coli K-12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)).

Key features of the MultiShot FlexPlate OmniMAX 2 T1R Competent Cells include:
Δ(ccdAB) for sensitivity to the toxic effects of the ccdB gene product, allowing negative selection of vectors containing the ccdB gene—ideal for transformation of Gateway and TOPO reactions
mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
tonA (fhuA) genotype to confer resistance to T1 and T5 phage
Highest transformation efficiency in FlexWell format (>5x108 cfu/µg control DNA)
endA1 for increased plasmid yield and quantity
recA1 for reduced occurrence of non-specific recombination in cloned DNA
lacZΔM15 for blue/white color screening of recombinant clones

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F´ {proAB lacIq lacZΔM15 Tn10(TetR ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

One Shot™ Stbl3™ Chemically Competent E. coli Invitrogen™

One Shot® Stbl3™ Chemically Competent E. coli are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower™ Lentiviral Expression Vectors and other retroviral vectors. The DNA yield from Stbl3™ cells is often >10-fold higher than alternative strains such as TOP10 (see table). One Shot® Stbl3™ Chemically Competent Cells deliver:

• Optimal performance for the propagation of lentiviral vectors
• Enhanced genomic cloning capacity (mcrB, mrr)
• Expected efficiency of >1 × 108 transformants/µg plasmid DNA
• High DNA yields

Recommended use
The Stbl3™ E. coli strain is derived from the HB101 E. coli strain and is recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., Invitrogen® ViraPower™ Lentiviral Expression kits). The transformation efficiency of One Shot® Stbl3™ chemically competent cells is greater than 1 × 108 cfu/µg plasmid DNA.

Genotype: F-mcrB mrrhsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ-leumtl-1

Find the strain and format that you need
We offer a variety of competent cells for the propagation of unstable DNA inserts.

MAX Efficiency® Stbl2™ Competent Cells—stable cloning of direct repeat and retroviral sequences and tandem array genes (5 × 200 µL) (>1 × 109 cfu/µg plasmid DNA)
One Shot® Stbl3™ Chemically Competent E. coli—cloning unstable inserts containing direct repeats (20 × 50 µL) (>1 × 108 cfu/µg plasmid DNA)
ElectroMAX™ Stbl4™ Competent Cells—high transformation efficiencies (>5 × 109 cfu/µg plasmid DNA), ideal for generating cDNA and genomic libraries and for cloning unstable inserts

MultiShot™ FlexPlate Stbl3™ Competent Cells Invitrogen™

MultiShot FlexPlate Stbl3 chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot FlexPlate Stbl3 competent E. coli cells are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower Lentiviral Expression vectors and other retroviral vectors. The DNA yield from Stbl3 cells is often >10-fold higher than alternative strains such as TOP10 (see table).

Reduce unwanted recombination with Stbl3 cells
MultiShot FlexPlate Stbl3 Competent Cells are derived from the HB101 E. coli strain and are recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., ViraPower Lentiviral Expression kits). The transformation efficiency of these cells is greater than 1 × 107 cfu/µg plasmid DNA.

Key features of the MultiShot FlexPlate Stbl3 Competent Cells include:
Optimal performance for the propagation of lentiviral vectors
mcrB, mrr for enhanced genomic cloning capacity
recA13 for reduced occurrence of non-specific recombination in cloned DNA
High DNA yields

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be transformed efficiently by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F-mcrB mrr hsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ- leu mtl-1

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli Invitrogen™

One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells currently available (see figure). Doubling time is approximately 50 minutes, compared to >74 minutes for other cloning strains. Mach1™ colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only 4 hours of growth from an overnight colony—saving an entire day in your experiments. The fast-growing Mach1™ T1R strain:

• Minimizes nonspecific recombination in cloned DNA (recA)
• Offers resistance to T1 and T5 phage (tonA)
• Yields cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
• Provides efficient transformation of unmethylated DNA from PCR amplifications (hsdR)
• Allows blue/white screening of recombinant clones (lacZΔM15)

Two formats to choose from
• The convenient One Shot® format provides single-use, 50-µL aliquots that eliminate efficiency-zapping, freeze-thaw cycles and money wasted on unused cells
• The MultiShot™ StripWell format allows rapid, high-throughput transformation in a 96-well stripwell plate, which allows you to perform as few or as many transformations as you need without wasting reagents

MultiShot™ StripWell Stbl3™ Competent Cells Invitrogen™

MultiShot StripWell Stbl3 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell Stbl3 competent E. coli cells are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower Lentiviral Expression vectors and other retroviral vectors. The DNA yield from Stbl3 cells is often >10-fold higher than alternative strains such as TOP10 (see table).

Reduce unwanted recombination with Stbl3 cells
MultiShot StripWell Stbl3 Competent Cells are derived from the HB101 E. coli strain and are recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., ViraPower Lentiviral Expression kits). The transformation efficiency of MultiShot StripWell Stbl3 Competent Cells is greater than 1 × 107 cfu/µg plasmid DNA.

Key features of the Stbl3 Competent Cells include:
Optimal performance for the propagation of lentiviral vectors
mcrB, mrr for enhanced genomic cloning capacity
recA13 for reduced occurrence of non-specific recombination in cloned DNA
High DNA yields

MultiShot StripWell format—flexible for no waste
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and to preventing contamination. After addition of DNA, MultiShot StripWell Competent Cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency.

Key features of the MultiShot StripWell format include:
Increase productivity—for medium throughput experimentation
Maximal flexibility—transform as few as 1 tube and as many as 96 from the StripWell rack
Familiar convenience—no aliquotting, add DNA directly to cells, heat shock, and recover in the same tube
Peace of mind—single-use transformation minimizes contamination

Genotype: F-mcrB mrr hsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ- leu mtl-1

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

MultiShot™ FlexPlate Mach1™ T1R Competent Cells Invitrogen™

MultiShot FlexPlate Mach1 T1R chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput. MultiShot FlexPlate Mach1 T1R competent E. coli cells are exceptionally fast-growing chemically competent cells, accelerating the time to clone evaluation.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Speedy Mach1 T1R cloning capabilities
MultiShot FlexPlate Mach1 T1R competent E. coli cells are fast growing. Mach1 colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only four hours of growth from an overnight colony—saving an entire day in your experiments. The Mach1 T1R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 108 cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate Mach1 T1R Competent Cells include:
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1398 for reduced occurrence of non-specific recombination in cloned DNA
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
tonA (fhuA) for T1 and T5 phage resistance
lacZΔM15 for blue/white color screening of recombinant clones

MultiShot FlexWell format—increase transformation productivity
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into any combination of 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be done quickly with a multichannel pipet or a liquid handling system. After addition of DNA, MultiShot FlexPlate Competent Cells can efficiently be transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key features of the MultiShot FlexPlate format include:
Increase throughput—compatible with automated liquid handling platforms
Flexible—transform in increments of 8 wells to entire 96-well plate
Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK-mK+) ΔrecA1398 endA1 tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

Sf21 cells in Sf-900™ II SFM Gibco™

Gibco® Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf21 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ II SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco® Sf21 cells (frozen in Gibco® Sf-900™ II SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf21 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Each vial containing 1 × 107 cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco® Sf-900™ II SFM are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.

Quality and performance testing
Each lot of Gibco® Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

One Shot™ INVαF´ Chemically Competent E. coli Invitrogen™

INVαF´ E. coliallow stable replication of high-copy number plasmids. The genotype of INVαF´ offers the following features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduction of homologous recombination of transformed plasmids (recA)
• Increased quality of plasmid DNA preparations (endA1)
• Production and rescue of single-strand DNA from vectors carrying the f1 origin of replication (F´)

T-REx™-293 Cell Line Invitrogen™

T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).

MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli Invitrogen™

MultiShot StripWell Mach1 T1R chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell Mach1 T1R competent E. coli cells are exceptionally fast-growing chemically competent cells, accelerating the time to clone evaluation.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Speedy Mach1 T1R cloning capabilities
MultiShot FlexPlate Mach1 T1R Competent Cells are fast growing. Mach1 colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only four hours of growth from an overnight colony—saving an entire day in your experiments. The Mach1 T1R strain is useful for many cloning applications and provides transformation efficiencies of >1 x 108 cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate Mach1 T1R Competent Cells include:
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1398 for reduced occurrence of non-specific recombination in cloned DNA
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
tonA (fhuA) for T1 and T5 phage resistance
lacZΔM15 for blue/white color screening of recombinant clones

MultiShot StripWell format—flexible for no waste
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and preventing contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency.

Key features of the MultiShot StripWell format include:
Increased productivity—for medium throughput experimentation
Maximal flexibility—transform as few as 1 tube and as many as 96 from the StripWell rack
Familiar convenience—no aliquotting, add DNA directly to cells, heat shock, and recover in the same tube
Peace of mind—single-use transformation minimizes contamination

Genotype: F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK-mK+) ΔrecA1398 endA1 tonA

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

Tali™ Viability Kit - Dead Cell Red Invitrogen™

The Tali® Viability Kit - Dead Cell Red is a ready-to-use solution of propidium iodide (PI) that has been validated for use with the Tali® Image-Based Cytometer. PI is a live cell-impermeant fluorogenic DNA-binding dye that has been extensively used to identify necrotic cells.

Key Features of Tali® Viability Kit - Dead Cell Red:

• Impermeant to live cells
• Easily enters and binds to nucleic acids in necrotic cells

After staining a cell population using Dead Cell Red™, dead cells display red fluorescence upon excitation. Tali® Viability Kit - Dead Cell Red can be used to identify dead cells in a population of unstained cells, or in a population of GFP-expressing cells.

One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells Invitrogen™

DH10B T1 Phage-Resistant E. colihave all the benefits of the DH10B genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination. The DH10B-T1R genotype has the following features:

tonA genotype to confer resistance to T1 and T5 phage
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)
• The endA1 mutation to increase plasmid yield and quantity
ZΔM15 for blue/white screening of recombinant clones

DH10B-T1R E. coliare available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High efficiency transformation is seen with plasmids 150 kb in size (3).

One Shot™ TOP10 Chemically Competent E. coli Invitrogen™

One Shot® TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 109 cfu/µg plasmid DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits. One Shot® TOP10 cells:

• Maximize cloning efficiency in a single-tube format
• Provide enhanced genomic DNA cloning capabilities

Easy-to-use One Shot® format
The single-tube, single-use format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping to save time and to prevent contamination.

Versatile cloning capabilities
One Shot® TOP10 E. coli cells are similar to the DH10B™ strain, and offer the following features:

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Genotype: F- mcrA Δ( mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ( araleu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require high-throughput transformation, choose from our collection of MultiShot™ formatted comp cells.

MAX Efficiency™ DH5αF´IQ Competent Cells Invitrogen™

MAX Efficiency DH5αF´IQ Chemically Competent Cells are high-efficiency chemically competent cells available for M13 cloning, cloning into plasmids with an f1-like origin of replication (phagemids), or cloning into any vector that uses expression from the lac promoter.
MAX Efficiency DH5αF´IQ cells offer the following features:

• >3 x 108 transformants/µg efficiency
• Stable F´ episome eliminates need for growth on minimal media
• ZΔM15 for blue/white screening of colonies or plaques

Three milliliters of lawn cells and a vial of monomer M13mp18 RF DNA are supplied as a control.
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