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Competent Cells
Cell competence refers to the ability of a cell to take up extracellular DNA from the environment via transformation. These products include competent cells for subcloning, protein expression, and genetic library creation.
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One Shot™ BL21(DE3) Chemically Competent E. coli Invitrogen™
One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. This strain had been engineered to carry the λDE3 lysogen that contains T7 RNA polymerase gene under control of the lacUV5 promoter.
BL21 Star™ (DE3)pLysS One Shot™ Chemically Competent E. coli Invitrogen™
One Shot BL21 Star (DE3)pLysS Chemically Competent E. coli are cells designed for applications that require high-level expression of non-toxic recombinant proteins from low or high copy number vectors, such as pRSET and pET. The BL21 (DE3) E.
One Shot™ BL21-AI™ Chemically Competent E. coli Invitrogen™
One Shot BL21-AI Chemically Competent E. coli cells are designed for applications that require tight regulation and strong expression of toxic proteins from any T7-promoter-based expression systems. A derivative of the E.
One Shot™ BL21(DE3)pLysE Chemically Competent E. coli Invitrogen™
One Shot BL21(DE3)pLysE Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. The pLysE plasmid carried by the BL21(DE3)pLysE strain produces higher amounts of T7 lysozyme than pLysS to further reduce basal level...
One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli Invitrogen™
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
One Shot™ BL21(DE3)pLysS Chemically Competent E. coli Invitrogen™
One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. The pLysS plasmid carried by the BL21(DE3) pLysS strain produces T7 lysozyme, an inhibitor of T7 RNA polymerase, to reduce basal level...
MultiShot™ StripWell BL21 Star™ (DE3) Competent Cells Invitrogen™
MultiShot StripWell BL21 Star (DE3) chemically competent E. coli cells are cloning competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems.
One Shot™ TOP10 Electrocomp™ E. coli Invitrogen™
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ µg supercoiled DNA, these cells allow for stable replication of high copy number plasmids. TOP10 Electrocomp E.
MultiShot™ FlexPlate Stbl3™ Competent Cells Invitrogen™
MultiShot FlexPlate Stbl3 Chemically Competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments to allow for flexibility in transformation...
TOP10 Electrocomp™ Kit Invitrogen™
TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and...
TOP10F´ Electrocomp™ Kit Invitrogen™
TOP10F´ Electrocomp Kit E. coli cells are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication.
Library Efficiency™ DH5α Competent Cells Invitrogen™
Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are able to achieve transformation efficiencies of >1 x 108 cfu/µg control DNA...
Subcloning Efficiency™ DH5α Competent Cells Invitrogen™
Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any general...
One Shot™ TOP10 Chemically Competent E. coli Invitrogen™
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/µg plasmid DNA. These cells allow stable replication of high-copy number plasmids and are the same competent cells that come...
ElectroMAX™ DH5α-E Competent Cells Invitrogen™
ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. It is challenging to achieve high transformation efficiency by electroporation using the standard DH5α strain (chemical transformation is more efficient) thus additional...
