Protein modification reagents are used to modify the amino acid side chains of proteins to alter their native charges, block or expose reactive binding sites, inactivate functional groups, and change functional groups to create targets for crosslinking and labeling.
Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions.
Thermo Scientific Pierce Iodoacetic Acid (IAA) can react with several protein functional groups but is typically used for specific S-carboxymethylation of sulfhydryls (reduced cysteines). Features of Pierce Iodocetic Acid (IAA): • React at slightly alkaline pH for specific S-carboxymethylation of...
Thermo Scientific Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.
Thermo Scientific Pierce Premium Grade TCEP-HCl is our highest quality formulation of this disulfide reducing agent, specially characterized for applications where product integrity and risk minimization are paramount.
Thermo Scientific Pierce SAT(PEG)4 is a long-arm (18.2 angstroms), pegylated form of SATA, a sulfhydryl-addition reagent for covalent modification of primary amines to facilitate controlled, heterobifunctional crosslinking.
Thermo Scientific Pierce Iodoacetamide or Chloroacetamide are provided in a no-weigh format, where small amounts of reagent are pre-weighed and packaged for single use. This single-use format prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and...
Thermo Scientific Pierce Traut's Reagent (2-iminothiolane) is a small thiolation compound that reacts with primary amines (e.g., lysine side chains) to add a small spacer arm (8.1 angstroms) terminated by a free sulfhydryl group.
Immobilized TCEP Disulfide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent. Immobilized TCEP Disulfide Reducing Gel may be adapted conveniently to a variety of scales and formats (i.e.
Thermo Scientific Pierce Sulfo-NHS-Acetate is a small compound used for blocking primary amines (e.g., lysine side chains). This compound forms stable, covalent amide bonds with amine groups to prevent specific interactions or conjugations.
Thermo Scientific Pierce MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) activated as NHS esters for covalent pegylation of primary amines on proteins (e.g., lysines) or assay surfaces.
Thermo Scientific Pierce CA(PEG)24 is a pegylated amino acid of the form carboxy-PEG-amine, containing 24 polyethylene glycol units, and is useful for a variety of surface- and molecule-pegylation applications.
Thermo Scientific Pierce MM(PEG)12 is a methyl-terminated, polyethylene glycol compound (12 PEG units) activated with a maleimide group for covalent pegylation of sulfhydryls on proteins (e.g., cysteines) or assay surfaces.
Thermo Scientific Pierce Immobilized Pepsin Agarose consists of an acidic endopeptidase (pepsin) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
Thermo Scientific Pierce SATP is a long-chain (4.1 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.