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Zeba™ Spin Desalting Columns, 7K MWCO, 10 mL Thermo Scientific™

Thermo Scientific Zeba Spin Desalting Columns, 7K MWCO, 10 mL are polypropylene devices containing a proprietary high-performance size-exclusion chromatography resin that provides an excellent protein desalting and recovery in a centrifuge format.

Features of Zeba Spin Desalting Columns, 7K MWCO, 5 mL:

High recovery—low-binding resin maximizes protein recovery
Fast—no fraction screening or waiting for protein to emerge by gravity-flow
Easy-to-use—no cumbersome column preparation or equilibration
Flexible—available in spin columns, filter spin plates and cartridges for a range of needs
Economical—great performance at lower cost than other commercially available cartridges

Even very dilute (25 µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (< 1000 MW) and good recovery of proteins and other macromolecules (> 7000 MW).

Available Formats:
Spin Columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
Chromatography Cartridges—compatible with automated liquid chromatography systems and with syringe processing (learn more about our Chromatography Cartridges)
96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.

More Product Data
Benchmarking experiments with Zeba Spin Desalting Columns
Chromatography cartridges for desalting and affinity purification

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Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL
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Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL

Slide-A-Lyzer™ Cassette Float Buoys (for 12 mL cassettes) Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Cassette Float Buoys are designed for floating 12 mL cassettes in dialysate.
These accessories are for use with Slide-A-Lyzer Dialysis Cassettes to remove low molecular weight contaminants and perform buffer exchange, desalting and concentration.

Features of Slide-A-Lyzer Cassette Float Buoys:

• Keeps stir bar from tearing dialysis membrane
• Matched to Slide-A-Lyzer Cassette for a secure fit
• Chemically inert

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Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 12 mL

Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 0.5 mL
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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 3 mL size is convenient for many applications and the box of five is designed for the budget conscious laboratory

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 0.1 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 3.5K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >3.5kDa in volumes from 10 µL to 2 mL.

Features of Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 2 mL:

Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

These 3.5K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated to 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data
New dimensions for low-volume sample dialysis
Separation characteristics of dialysis membranes

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SnakeSkin™ Dialysis Tubing, 10K MWCO, 16 mm Thermo Scientific™

Thermo Scientific SnakeSkin Dialysis Tubing, 10K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 10K MWCO, 16mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

10K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: 21%
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 10K MWCO, is composed of regenerated-cellulose and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat-width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.1 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 10K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >10kDa in volumes from 10 µL to 2 mL.

Features of Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.1 mL:

Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
100% leak-tested – innovative design does not permit 'wicking' that can occur in home-made devices
Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

These 10K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data

Zeba™ Spin Desalting Columns, 40K MWCO, 2 mL Thermo Scientific™

Thermo Scientific Zeba Spin Desalting Columns, 40K MWCO, 2 mL are polypropylene devices containing a proprietary high-performance size-exclusion chromatography resin that provides an excellent protein desalting and recovery in a centrifuge format.

Features of Zeba Spin Desalting Columns, 40K MWCO, 2 mL:

High recovery—low-binding resin maximizes protein recovery
Fast—no screening of fractions or waiting for protein to emerge by gravity-flow
Easy-to-use—no cumbersome column preparation or equilibration
Flexible—available in spin columns and filter spin plates for a range of needs
Economical—comparable performance at lower cost than other commercially available resins

Dilute (25 µg/mL) protein samples can be successfully processed to achieve greater than 90% retention (removal) of salts and other small molecules (< 2000 MW) while maintaining and good recovery of proteins and other macromolecules (> 40,000 MW).

Available Formats:
Spin Columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.

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Pierce™ Dextran Desalting Columns, 5K MWCO, 5 mL Thermo Scientific™

Thermo Scientific Pierce Dextran Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low-molecular weight buffer salts and reagents.

Pierce Dextran Desalting Columns (5K MWCO) are plastic columns that are pre-packed with crosslinked beaded dextran for protein desalting and buffer-exchange applications based on a molecular-weight cutoff (MWCO) of 5000. The wet diameter of these dextran desalting beads is 50 to 150 µM. The resin has good rigidity for easy handling and excellent flow properties. Dextran is stable in water, salt solutions, organic solvents and alkaline or weakly acidic solutions. It is heat-stable and can be autoclaved dry or in solution at a neutral pH for 30 minutes at 120°C without affecting its chromatographic properties.

Features of Dextran Resin:

• Stable in water, salt solutions, organic solvents and alkaline or acidic conditions
• Excellent flow properties
• Heat-stable

Applications
• Removing salts from protein solutions
• Removing phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Removing unreacted dye from fluorescent antibodies
• Removing free radiolabel from labeled proteins
• Exchanging one buffer for another

Gravity-flow gel filtration involves the chromatographic separation of molecules of different dimensions based ontheir relative abilities to penetrate into a suitable stationary phase. A chromatographic matrix, usually consisting of very small, uncharged porous particles in an aqueous solution, is packed into a column and then used for the separation. Different levels of separation can be achieved based on the pore size of the medium packed into the column. The medium can be chosen to totally exclude proteins or large molecules, while still including small solutes. Large molecules are excluded from the internal pores of the gel and emerge first from the column. The smaller molecules are able to penetrate the pores, then progress through the column at a slower rate. These smaller molecules are subsequently flushed through the column with additional buffer volume.

Desalting and buffer exchange are two of the most widely used applications of gel filtration chromatography.

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Slide-A-Lyzer™ Dialysis Flasks, 2K MWCO, 250 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Flasks, 2K MWCO, 250 mL facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules in sample volumes up to 250 mL.

Features of Slide-A-Lyzer Dialysis Flasks, 2K MWCO, 250 mL:

Easy to use – Simply pipette or pour sample into flask and begin dialysis
Fast dialysis – flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 250 mL sample in 8 hours to overnight
High recovery – rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
Multiple molecular-weight cut-offs – select the membrane MWCO that best suits your sample's molecular weight
Color-coded frames – easily identify membrane pore size (MWCO) based on the frame color

Dialysis Membrane Specifications:
• Composition: Regenerated-cellulose
• Thickness: 0.9 to 1.2mil (22.5 to 30μm); 2K MWCO = 2mil (50μm)
• Hydration Required Before Use: 30 seconds to 2 minutes
• Glycerol Content: Trace (10K MWCO content is 21%)
• Sulfur Content: 0.04 to 0.17%
• Heavy metals Content: Trace

Slide-A-Lyzer Dialysis Flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: Purple (20,000 daltons), Orange (10,000 daltons), Pink (3500 daltons), and Blue (2000 daltons).

Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation and temperature. Minimum dialysis time must be determined empirically for each specific system.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

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SnakeSkin™ Dialysis Clips Thermo Scientific™

Thermo Scientific SnakeSkin Dialysis Tubing Clips are sturdy, non-floating tube closures recommended for use with SnakeSkin Dialysis Tubing and other suitable dialysis tubing with flat tube diameters up to 50mm.

Features of SnakeSkin Dialysis Tubing Clips:

Durable—polyamide composition for long-life
Dove-tail latch—prevent sample loss
Heat resistant—autoclave up to 134°C

Dialysis Clip Specifications:
Height: 10mm
Width: 16mm
Inner Length: 50mm (maximum closure width)
Outer Length: 66mm (end to end)
Composition: Polyamide (nylon) derivative

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Pierce™ Peptide Desalting Spin Columns Thermo Scientific™

Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. The polymer-based hydrophobic resin contained in each column provides excellent binding and recovery characteristics for peptide samples in preparation for mass spectrometry and other methods.

Features of Pierce Peptide Desalting Spin Columns include:
Removes MS-interfering contaminants—significantly reduces signal suppression and improves sensitivity in mass spectrometry
High binding capacity—special hydrophobic polymer-based resin allows for excellent recovery of peptide loads of 5 µg to as high as 5 mg of native and TMT-labeled peptides
Easy to use—resin is provided in single-use spin column format that fits many common 2 mL tubes
Compatible—resin is validated with a variety of complex samples, including TMT-labeled peptides, and is stable under extreme pH conditions

Pierce Peptide Desalting Spin Columns provide a convenient and reproducible way to desalt and remove contaminants from peptide samples prior to analysis by mass spectrometry. Each spin column can bind from 5 µg to 5 mg of native and TMT-labeled peptides. The spin column format allows processing of multiple samples (10 to 300 µL each) in parallel in approximately 30 minutes.

Mass spectrometry is essential for studying biological compounds. However, many of the buffers and reagents used in the course of sample preparation will interfere with mass spectrometry analysis, leading to poor-quality spectra and reduced sensitivity. Pierce Peptide Desalting Spin Columns are designed to remove many of the interfering hydrophilic sample contaminants with minimal sample loss, thus enabling sensitive and comprehensive analysis of digested protein samples by mass spectrometry.

Slide-A-Lyzer™ G2 Dialysis Cassettes, 10K MWCO, 30 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes

10K Dialysis Membrane Specifications
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be loaded with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules that are larger than 10,000 daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free. The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL.

Gamma-irradiated (Sterile) Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

More Product Data
Separation characteristics of dialysis membranes

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