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Slide-A-Lyzer™ Cassette Syringes, 5 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassette Syringes are designed for efficiently loading samples into Slide-A-Lyzer cassettes and removing the sample after dialysis. The 5 mL syringes are a convenient size for use with 3 mL cassettes (66380, 66381)

Features of Slide-A-Lyzer Dialysis Syringes:

• Matched to the Slide-A-Lyzer to minimize losses
• Chemically inert - will not contaminate product
• Appropriately sized for different sample volumes

These accessories are for use with Slide-A-Lyzer Dialysis Cassettes to remove low molecular weight contaminants and perform buffer exchange, desalting and concentration.

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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL

Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 12 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (7K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 7000 daltons.
The 12 mL size is convenient for many preparative applications and the kit format contains syringes, needles, and float buoys for convenience.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis —flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Three cassette sizes —select the cassette that best suits your sample volume
Color-coded —green cassette frame specifies cassettes with the 7000 MW pore size membrane

7K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 13%
Sulfur Content: 0.1 to 0.15%
Heavy Metals Content: Trace

Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12 mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL
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Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 12 mL

Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 0.5 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (3.5K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 3,500 daltons.
The 0.5 mL size is convenient for low volume samples

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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POROS™ GoPure™ XS Pre-packed Column, 0.5 x 5 cm, 1 mL Thermo Scientific™

GoPure™ 0.5 cmD pre-packed columns are intended for the screening of POROS™ ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ XS Column has a sulphopropyl functional group for strong cation exchange chromatography.

• Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 ml/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cmD Operating Instructions below.

Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

Zeba™ Spin Desalting Columns, 40K MWCO, 10 mL Thermo Scientific™

Thermo Scientific Zeba Spin Desalting Columns, 40K MWCO, 10 mL are polypropylene devices containing a proprietary high-performance size-exclusion chromatography resin that provides an excellent protein desalting and recovery in a centrifuge format.

Features of Zeba Spin Desalting Columns, 40K MWCO, 10 mL:

High recovery—low-binding resin maximizes protein recovery
Fast—no screening of fractions or waiting for protein to emerge by gravity-flow
Easy-to-use—no cumbersome column preparation or equilibration
Flexible—available in spin columns and filter spin plates for a range of needs
Economical—comparable performance at lower cost than other commercially available resins

Dilute (25 µg/mL) protein samples can be successfully processed to achieve greater than 90% retention (removal) of salts and other small molecules (< 2000 MW) while maintaining and good recovery of proteins and other macromolecules (> 40,000 MW).

Available Formats:
Spin Columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.

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POROS™ XQ Strong Anion Exchange Resin Thermo Scientific™

POROS™ XQ resin is a next–generation, high-capacity, high-resolution, salt–tolerant, strong anion exchange resin that that enables >140 mg/mL dynamic binding capacity in the presence of up to 6.6 mS/cm NaCl, while delivering exceptional separation performance. A major improvement to the performance of traditional anion exchangers, POROS™ XQ resin sets the standard for strong anion exchange chromatography in both bind & elute or flow-through modes of operation.

Features of POROS™ XQ resin include:
• High dynamic binding capacity leading to reduced column size, smaller footprint, decreased water and buffer usage, and reduced cycling
• High resolution supporting improved product yield and purity, ultimately improving the cost of goods for the molecule being produced
• Robust salt tolerance allowing enhanced process design flexibility and streamlining of process flow
• Low operating back pressure and linear pressure versus flow responses driving flexible scalability
• Rigid polymeric bead with covalent surface chemistry for easier handling and packing, and superior physical and chemical stability enabling a more robust downstream purification process

Dynamic Binding Capacity
POROS™ XQ resin has a high binding capacity of more than 140 mg/mL at 5% breakthrough, over a broad range of process conditions, with superior binding capacity across a broad range of pH and salt conditions. Higher dynamic binding capacity was achieved by optimizing the base bead pore structure and surface chemistry.

High Resolution
Using an optimized 50 µm particle size, POROS™ XQ resin maintains the superior resolving capacity of the POROS™ resins, overcoming the challenging impurity levels associated with high titer processes. Resolution remains consistent across a wide range of operating flow rates.

Robust Salt Tolerance
POROS™ XQ resin has high and consistent protein capacity across a broad range of salt concentrations up to 140 mg/mL at 50 mM NaCl (6.6 mS/cm conductivity). The ability to load columns under higher conductivity reduces or helps eliminate the dilution of column feed streams or the removal of unit operations, such as tangential flow filtration.

Zeba™ Desalting Chromatography Cartridges, 7K MWCO, 1 mL Thermo Scientific™

Thermo Scientific Pierce Zeba Desalting Chromatography Cartridges, 7K MWCO, 2 mL contains Zeba™ High-Performance Resin for desalting or buffer exchange applications. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin and other small labeling reagents. Protein samples can be processed with unsurpassed protein recovery and ≥95% retention of salts and other small molecular contaminants (< 1,000 Da).

Features of Zeba Desalting Chromatography Cartridges, 7K MWCO, 2 mL:

High recovery—low-binding resin maximizes protein recovery
Fast—no fraction screening or waiting for protein to emerge by gravity-flow
Easy-to-use—no cumbersome column preparation or equilibration
Flexible—available in spin columns, filter spin plates and cartridges for a range of needs
Economical—great performance at lower cost than other commercially available cartridges

Zeba Desalting Cartridge-fittings are convenient, ready-to-use devices compatible with the major automated liquid-chromatography systems or for manual syringe processing. The cartridges attach directly to ÄKTA™ or FPLC Systems without additional connectors. An accessory pack included with each product readily adapts cartridges for use with Luer Syringe Fittings or 1/16” tubing. Pierce Desalting Cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses and efficiently process samples from 50 to 1500 µL.

Available Formats:
Spin Columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
Chromatography Cartridges—compatible with automated liquid chromatography systems and with syringe processing (learn more about our Chromatography Cartridges)
96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.

More Product Data
Benchmarking experiments with Zeba Spin Desalting Columns
Chromatography cartridges for desalting and affinity purification

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Slide-A-Lyzer™ Dialysis Flotation Disks Thermo Scientific™

Handy floatation disks are for use with each Thermo Scientific Slide-A-Lyzer Dialysis Flask. When attached to the neck of the flask, the floatation disk allows the flask to float in a vertical position for simple and safe handling.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL
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Slide-A-Lyzer™ Cassette Syringes, 20 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassette Syringes are designed for efficiently loading samples into Slide-A-Lyzer cassettes and removing the sample after dialysis. The 20 mL syringes are a convenient size for use with 12 mL cassettes (66810, 66811)

These accessories are for use with Slide-A-Lyzer Dialysis Cassettes to remove low molecular weight contaminants and perform buffer exchange, desalting and concentration.

Features of Slide-A-Lyzer Dialysis Syringes:

• Matched to the Slide-A-Lyzer to minimize losses
• Chemically inert - will not contaminate product
• Appropriately sized for different sample volumes

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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 3 mL size is convenient for many applications and the box of five is designed for the budget conscious laboratory

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL Thermo Scientific™

Thermo Scientific Pierce 1.8K MWCO (molecular-weight cutoff) Polyacrylamide Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low-molecular-weight buffer salts and reagents.

Pierce 1.8K MWCO Polyacrylamide Desalting Columns are pre-packed with porous polyacrylamide beads and provide for buffer-exchange and desalting of proteins and other macromolecular samples based on a molecular-weight cutoff (MWCO) of 1800. Unlike other size-exclusion media, polyacrylamide resin is not subject to enzymatic degradation and will not serve as a nutrient for microbial growth. The resin is very hydrophilic, thereby minimizing undesirable binding interactions between the resin and sample molecules. Contamination with low molecular weight sugars (which may occur with crosslinked dextran resin) is not a concern when using polyacrylamide resin. Oxidizing agents can be removed without destroying the support. This resin is susceptible to hydrolysis of amide groups under extreme pH conditions, so an operating pH of 2-10 is recommended at room temperature. The polyacrylamide resin can also be autoclaved at pH 5.5-6.5 for 30 minutes at 120°C.

These columns use a resin whose beads have a wet diameter of 45 to 90 µM, and are ideal for separating peptides and small macromolecules (greater than 1.8kDa) from buffer salts and other compounds (less than 500 MW).

Features of Polyacrylamide Resin:
• Stable in water, salt solutions, organic solvents and alkaline or acidic conditions
• Excellent flow properties
• Heat-stable

Applications:
• Removing salts from protein solutions
• Removing phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Removing unreacted dye from fluorescent antibodies
• Removing free radiolabel from labeled proteins
• Exchanging one buffer for another

Gravity-flow resin filtration involves the chromatographic separation of molecules of different dimensions based on their relative abilities to penetrate into a suitable stationary phase. A chromatographic matrix, usually consisting of very small, uncharged porous particles in an aqueous solution, is packed into a column and then used for the separation. Different levels of separation can be achieved based on the pore size of the medium packed into the column. The medium can be chosen to totally exclude proteins or large molecules, while still including small solutes. Large molecules are excluded from the internal pores of the resin and emerge first from the column. The smaller molecules are able to penetrate the pores, then progress through the column at a slower rate. These smaller molecules are subsequently flushed through the column with additional buffer volume.

Desalting and buffer exchange are two of the most widely used applications of resin filtration chromatography.

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SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 16 mm Thermo Scientific™

Thermo Scientific SnakeSkin Dialysis Tubing, 3.5K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 3.5K MWCO, 16mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

3.5K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: Trace
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 3.5K MWCO, is composed of regenerated-cellulose dialysis tubing and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

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96-well Deep-well Plate, 2.2mL, for Pierce™ 96-well Microdialysis Thermo Scientific™

The Thermo Scientific Pierce 96-well Deep-well Plate, 2.2 mL, is a polypropylene deep-well assay plate suficient for 96 microdialysis devices.

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Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

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POROS™ GoPure™ AAVX Pre-packed Column, 0.5 x 5 cm, 1 mL Thermo Scientific™

GoPure 0.5-cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS CaptureSelect AAVX 50 µm resin in this GoPure column has a novel AAVX CaptureSelect ligand, making it suited primarily for affinity capture of a broad range of adeno-associated virus (AAV) serotypes.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure family of pre-packed columns simplifies process scale-up and process modeling

GoPure 0.5-cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1-mL bed volume reduces sample requirements, and the recommended > 3-minute residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the GoPure 0.5-cmD Operating Instructions below.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.
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