Shop All Dialysis and Desalting

Pierce™ Dextran Desalting Columns, 5K MWCO, 5 mL Thermo Scientific™

Thermo Scientific Pierce Dextran Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low-molecular weight buffer salts and reagents.

Pierce Dextran Desalting Columns (5K MWCO) are plastic columns that are pre-packed with crosslinked beaded dextran for protein desalting and buffer-exchange applications based on a molecular-weight cutoff (MWCO) of 5000. The wet diameter of these dextran desalting beads is 50 to 150 µM. The resin has good rigidity for easy handling and excellent flow properties. Dextran is stable in water, salt solutions, organic solvents and alkaline or weakly acidic solutions. It is heat-stable and can be autoclaved dry or in solution at a neutral pH for 30 minutes at 120°C without affecting its chromatographic properties.

Features of Dextran Resin:

• Stable in water, salt solutions, organic solvents and alkaline or acidic conditions
• Excellent flow properties
• Heat-stable

Applications
• Removing salts from protein solutions
• Removing phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Removing unreacted dye from fluorescent antibodies
• Removing free radiolabel from labeled proteins
• Exchanging one buffer for another

Gravity-flow gel filtration involves the chromatographic separation of molecules of different dimensions based ontheir relative abilities to penetrate into a suitable stationary phase. A chromatographic matrix, usually consisting of very small, uncharged porous particles in an aqueous solution, is packed into a column and then used for the separation. Different levels of separation can be achieved based on the pore size of the medium packed into the column. The medium can be chosen to totally exclude proteins or large molecules, while still including small solutes. Large molecules are excluded from the internal pores of the gel and emerge first from the column. The smaller molecules are able to penetrate the pores, then progress through the column at a slower rate. These smaller molecules are subsequently flushed through the column with additional buffer volume.

Desalting and buffer exchange are two of the most widely used applications of gel filtration chromatography.

Related Products
Pierce™ Dextran Desalting Columns, 5K MWCO, 10 mL

Competition Rapid Equilibrium Dialysis (RED) Base Plate Thermo Scientific™

Thermo Scientific Pierce Competition RED Device Inserts and Base Plates comprise a specially designed system for evaluating competitive binding interactions using rapid equilibrium dialysis (RED).

The Competition RED Device was developed in association with pharmaceutical laboratories to more accurately model in vivo drug interactions by enabling equilibrium dialysis experiments to be performed in a multiplexed format involving competition for binding among different tissues. The Competition RED Device System consists of disposable dialysis tube inserts and a reusable Base Plate made of high-grade PTFE. The Base Plate is much like our regular RED Device Plate except that it is divided into different size chambers (wells) for positioning 2-8 RED Device Inserts per well. The format enables competitive dialysis experiments involving 2-15 separate tissue or protein fluid samples. Each Competition RED Insert contains either one or two separate dialysis chambers (each package includes a selection of both types). The Competition RED Device requires no extensive assembly or specialized equipment, and each chamber/well is easily accessible from the top of the device.

Features of the Competition Rapid Equilibrium Dialysis (RED) Device:

Easy to use—disposable tubes require no presoaking step, assembly, or specialized equipment
Short incubation time—design provides high surface-to-volume ratio of membrane to sample, enabling equilibrium to be reached within 2-4 hours
Flexible format—base plate contains several different chamber sizes, enabling small molecule partitioning studies involving 2-16 tissue or protein samples without waste
Robust—compartmentalized design eliminates potential for cross talk or leakage
Reproducible and accurate—perform controlled experiments with multiple tissues to obtain screening results that have high predictability for in vivo studies with animal models
Validated quality—base plate is composed of chemically inert high-grade PTFE, eliminating non-specific binding and risk of contamination; each lot is functionally tested in a protein binding assay for guaranteed performance

Applications:
• ADME-Tox studies: in vitro screening of drug partitioning between plasma and multiple tissues before in vivo studies with animal models
• Aids in determining formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Competitive binding and dissociation constant determination for small molecules versus multiple targets

Determining the extent to which a molecule partitions between plasma and specific tissues in the body is a critical phase of drug development because it determines compound dosing, efficacy, clearance rate, and the potential for drug interactions or tissue damage. Although final assessment of these variables invariably requires animal dosing and analysis, in vitro screening systems that are rapid and have good predictability are highly desirable for ADME-Tox studies. Equilibrium dialysis is among the most successful of in vitro screening method for evaluating drug binding and dosage requirements. Equilibrium dialysis is used to estimate the non-bound drug fraction in plasma or drug partitioning between red blood cell and plasma, plasma and liver microsomes, or plasma and tissue homogenates. However, many tissue-partitioning studies indicate that certain drugs show significant binding to tissues tested individually but have considerably different binding preferences when placed in competition between multiple tissues. The Competition RED Device extends the power of equilibrium dialysis by facilitating analysis of simultaneous drug interactions and partitioning among multiple tissues.

Posters:
• (Xia, C. et al.)
• (Qian, M.G., et al.)
• (Matuszak, K. and Custer, C.)

Related Products
Competition Rapid Equilibrium Dialysis (RED) Inserts

Competition Rapid Equilibrium Dialysis (RED) Inserts Thermo Scientific™

Thermo Scientific Pierce Competition RED Device Inserts and Base Plates comprise a specially designed system for evaluating competitive binding interactions using rapid equilibrium dialysis (RED).

The Competition RED Device was developed in association with pharmaceutical laboratories to more accurately model in vivo drug interactions by enabling equilibrium dialysis experiments to be performed in a multiplexed format involving competition for binding among different tissues. The Competition RED Device System consists of disposable dialysis tube inserts and a reusable Base Plate made of high-grade PTFE. The Base Plate is much like our regular RED Device Plate except that it is divided into different size chambers (wells) for positioning 2-8 RED Device Inserts per well. The format enables competitive dialysis experiments involving 2-15 separate tissue or protein fluid samples. Each Competition RED Insert contains either one or two separate dialysis chambers (each package includes a selection of both types). The Competition RED Device requires no extensive assembly or specialized equipment, and each chamber/well is easily accessible from the top of the device.

Features of the Competition Rapid Equilibrium Dialysis (RED) Device:

Easy to use—disposable tubes require no presoaking step, assembly, or specialized equipment
Short incubation time—design provides high surface-to-volume ratio of membrane to sample, enabling equilibrium to be reached within 2-4 hours
Flexible format—base plate contains several different chamber sizes, enabling small molecule partitioning studies involving 2-16 tissue or protein samples without waste
Robust—compartmentalized design eliminates potential for cross talk or leakage
Reproducible and accurate—perform controlled experiments with multiple tissues to obtain screening results that have high predictability for in vivo studies with animal models
Validated quality—base plate is composed of chemically inert high-grade PTFE, eliminating non-specific binding and risk of contamination; each lot is functionally tested in a protein binding assay for guaranteed performance

Applications:
• ADME-Tox studies: in vitro screening of drug partitioning between plasma and multiple tissues before in vivo studies with animal models
• Aids in determining formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Competitive binding and dissociation constant determination for small molecules versus multiple targets

Determining the extent to which a molecule partitions between plasma and specific tissues in the body is a critical phase of drug development because it determines compound dosing, efficacy, clearance rate, and the potential for drug interactions or tissue damage. Although final assessment of these variables invariably requires animal dosing and analysis, in vitro screening systems that are rapid and have good predictability are highly desirable for ADME-Tox studies. Equilibrium dialysis is among the most successful of in vitro screening method for evaluating drug binding and dosage requirements. Equilibrium dialysis is used to estimate the non-bound drug fraction in plasma or drug partitioning between red blood cell and plasma, plasma and liver microsomes, or plasma and tissue homogenates. However, many tissue-partitioning studies indicate that certain drugs show significant binding to tissues tested individually but have considerably different binding preferences when placed in competition between multiple tissues. The Competition RED Device extends the power of equilibrium dialysis by facilitating analysis of simultaneous drug interactions and partitioning among multiple tissues.

Posters:
• (Xia, C. et al.)
• (Qian, M.G., et al.)
• (Matuszak, K. and Custer, C.)

Related Products
Competition Rapid Equilibrium Dialysis (RED) Base Plate

Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 2 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 10K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >10kDa in volumes from 10 µL to 2 mL.

Features of Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 2 mL:

Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
100% leak-tested – innovative design does not permit 'wicking' that can occur in home-made devices
Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

These 10K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data
New dimensions for low-volume sample dialysis
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device Kit, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device Kit, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.5 mL

Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 70 mL

Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 0.5 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (2K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 2,000 daltons.
The 0.5 mL size is convenient for low volume samples

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 3 mL
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Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 2 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 20K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >20kDa in volumes from 10 µL to 2 mL.

Features of Slide-A-Lyzer MINI Dialysis Device, 20K MWCO, 2 mL:

Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
100% leak-tested – innovative design does not permit 'wicking' that can occur in home-made devices
Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

These 20K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data
New dimensions for low-volume sample dialysis
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 0.5 mL

Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 15 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 70 mL

Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.1 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 10K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >10kDa in volumes from 10 µL to 2 mL.

Features of Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.1 mL:

Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
100% leak-tested – innovative design does not permit 'wicking' that can occur in home-made devices
Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

These 10K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data

Slide-A-Lyzer™ Cassette Syringes, 1 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer Dialysis Cassette Syringes are designed for efficiently loading samples into Slide-A-Lyzer cassettes and removing the sample after dialysis. The 1 mL syringes are a convenient size for use with 0.5 mL cassettes (66383, 66384)

These accessories are for use with Slide-A-Lyzer Dialysis Cassettes to remove low molecular weight contaminants and perform buffer exchange, desalting and concentration.

Features of Slide-A-Lyzer Dialysis Syringes:

• Matched to the Slide-A-Lyzer to minimize losses
• Chemically inert - will not contaminate product
• Appropriately sized for different sample volumes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL

Zeba™ Spin Desalting Columns, 7K MWCO, 5 mL Thermo Scientific™

Thermo Scientific Zeba Spin Desalting Columns, 7K MWCO, 5 mL are polypropylene devices containing a proprietary high-performance size-exclusion chromatography resin that provides an excellent protein desalting and recovery in a centrifuge format.

Features of Zeba Spin Desalting Columns, 7K MWCO, 5 mL:

High recovery—low-binding resin maximizes protein recovery
Fast—no fraction screening or waiting for protein to emerge by gravity-flow
Easy-to-use—no cumbersome column preparation or equilibration
Flexible—available in spin columns, filter spin plates and cartridges for a range of needs
Economical—great performance at lower cost than other commercially available cartridges

Even very dilute (25 µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (< 1000 MW) and good recovery of proteins and other macromolecules (> 7000 MW).

Available Formats:
Spin Columns—75 µL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
Chromatography Cartridges—compatible with automated liquid chromatography systems and with syringe processing (learn more about our Chromatography Cartridges)
96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.

More Product Data
Benchmarking experiments with Zeba Spin Desalting Columns
Chromatography cartridges for desalting and affinity purification

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Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 µL
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Zeba™ Spin Desalting Columns, 7K MWCO, 10 mL

POROS™ XQ Strong Anion Exchange Resin Thermo Scientific™

POROS™ XQ resin is a next–generation, high-capacity, high-resolution, salt–tolerant, strong anion exchange resin that that enables >140 mg/mL dynamic binding capacity in the presence of up to 6.6 mS/cm NaCl, while delivering exceptional separation performance. A major improvement to the performance of traditional anion exchangers, POROS™ XQ resin sets the standard for strong anion exchange chromatography in both bind & elute or flow-through modes of operation.

Features of POROS™ XQ resin include:
• High dynamic binding capacity leading to reduced column size, smaller footprint, decreased water and buffer usage, and reduced cycling
• High resolution supporting improved product yield and purity, ultimately improving the cost of goods for the molecule being produced
• Robust salt tolerance allowing enhanced process design flexibility and streamlining of process flow
• Low operating back pressure and linear pressure versus flow responses driving flexible scalability
• Rigid polymeric bead with covalent surface chemistry for easier handling and packing, and superior physical and chemical stability enabling a more robust downstream purification process

Dynamic Binding Capacity
POROS™ XQ resin has a high binding capacity of more than 140 mg/mL at 5% breakthrough, over a broad range of process conditions, with superior binding capacity across a broad range of pH and salt conditions. Higher dynamic binding capacity was achieved by optimizing the base bead pore structure and surface chemistry.

High Resolution
Using an optimized 50 µm particle size, POROS™ XQ resin maintains the superior resolving capacity of the POROS™ resins, overcoming the challenging impurity levels associated with high titer processes. Resolution remains consistent across a wide range of operating flow rates.

Robust Salt Tolerance
POROS™ XQ resin has high and consistent protein capacity across a broad range of salt concentrations up to 140 mg/mL at 50 mM NaCl (6.6 mS/cm conductivity). The ability to load columns under higher conductivity reduces or helps eliminate the dilution of column feed streams or the removal of unit operations, such as tangential flow filtration.

POROS™ 50 HS Strong Cation Exchange Resin Thermo Scientific™

Applied Biosystems™ POROS™ Strong Cation Exchange Media. POROS™ 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

SnakeSkin™ Dialysis Tubing, 10K MWCO, 16 mm Thermo Scientific™

Thermo Scientific SnakeSkin Dialysis Tubing, 10K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 10K MWCO, 16mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

10K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: 21%
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 10K MWCO, is composed of regenerated-cellulose and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat-width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

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SnakeSkin™ Dialysis Clips

Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 30 mL Thermo Scientific™

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (20K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 20K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 20,000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 3 mL
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Slide-A-Lyzer™ G2 Dialysis Cassettes, 20K MWCO, 70 mL
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