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Gene Reporter Systems
Gene reporter systems are used to study gene expression and associated cellular processes. When gene reporters are expressed, they generate measurable, easily identifiable characteristics that can indicate whether a gene has been taken up or expressed.
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BLOCK-iT™ Pol II miR RNAi Expression Vector Kit Invitrogen™
Combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit (Green Fluorescence), for live-cell imaging Invitrogen™
Contains an expression tag-based fluorescence labeling reagent for live-cell labeling
BLOCK-iT™ Adenoviral RNAi Expression System Invitrogen™
Used for the efficient delivery and transient expression of a short hairpin RNA (shRNA) in vivo from an adenoviral vector
Biotin-14-dCTP Invitrogen™
Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling
Biotin-14-CTP Invitrogen™
A CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker
Employs a novel, non-enzymatic technology that rapidly depletes >95% of the alpha and beta globin mRNA from total RNA preparations derived from whole blood
Alexa Fluor™ 555-aha-dCTP Invitrogen™
For better resolution of two-color microarray gene expression assays
BLOCK-iT™ Lentiviral RNAi Gateway™ Vector Kit Invitrogen™
The pLenti6/BLOCK-iT-DEST expression vector provided in the BLOCK-iT Lentiviral RNAi Expression System can be used to efficiently introduce and stably express short hairpin RNA (shRNA) in vivo from a lentiviral vector
Human Cot-1 DNA™-Fluorometric QC Invitrogen™
Quantifying by fluorometry provides greater data accuracy
Thermo Scientific Luc-Screen Extended-Glow Luciferase Reporter Gene Assay System with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the
Yeast tRNA Invitrogen™
Supplied in lyophilized form
λ DNA (cIind 1 ts857 Sam 7) Invitrogen™
λ DNA (cIind 1 ts857 Sam 7) is isolated from a dam+ dcm+ E coli LE597 lysogen
RadPrime DNA Labeling System Invitrogen™
Ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for the detection of DNA and RNA
Random Primers DNA Labeling System Invitrogen™
Ideal for radioactively labeling DNA, particularly fragments under 1 kb
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Reporter genes are genes whose products can be readily assayed subsequent to transfection, and can be used as markers for screening successfully transfected cells, for studying regulation of gene expression, or serve as controls for standardizing transfection efficiencies.
Reporter gene assays are invaluable for studying the regulation of gene expression by cis- or trans-acting factors, or for measuring activation of cell signaling pathways. In these assays, the reporter gene acts as a surrogate for the coding region of the gene under study.
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Product Bulletin: Phospha-Light™ Secreted Placental Alkaline Phosphatase Reporter Gene Assay System (English )
Protocol: Phospha-Light™ System (English )