Gene reporter systems are used to study gene expression and associated cellular processes. When gene reporters are expressed, they generate measurable, easily identifiable characteristics that can indicate whether a gene has been taken up or expressed.
The TOPO™ TA Cloning™ Kits for Sequencing provide a highly efficient, 5-minute, one-step cloning strategy ('TOPO™ Cloning') for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing.
The pLenti6/BLOCK-iT™-DEST expression vector provided in the BLOCK-iT™ Lentiviral RNAi Expression System can be used to efficiently introduce and stably express short hairpin RNA (shRNA) in vivo from a lentiviral vector.
The BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit contains the pLenti4/BLOCK-iT™-DEST expression vector which enables lentiviral delivery and genomic integration of DNA coding for shRNA. Once expressed, the shRNA is processed by cellular machinery and initiates target-specific RNAi.
The Luc-Screen™ assay system with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the Luc-Screen™ assay from 50 fg to 100 ng of pure enzyme in culture medium.
The BLOCK-iT™ Pol II miR RNAi Expression Vector Kit with EmGFP combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript. The pcDNA™6.
Labeled 5-aminohexylacrylamido-dUTP (aha-dUTP) nucleotides are modified at the C-5 position of uridine with a unique hexylacrylamide linker, which serves as a spacer between the nucleotide and the dye.
Human Cot-1 DNA™-Fluorometric QC is placental DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences, such as the Alu and Kpn family members (1,2). Quantifying Cot-1 DNA™ by fluorometry provides more accurate concentration data of higher molecular weight products, making it...
The pLenti6/TR lentiviral vector can be used to make stable cell lines expressing high levels of tetracycline repressor (TR) protein under the control of the CMV promoter. This vector contains elements that allow packaging of the construct into lentiviral particles and the Blasticidin resistance...
The NovaBright™ Phospha-Light™ EXP Assay Kit is a second generation chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase (SEAP) or the non-secreted placental isozyme PLAP.
The FluoReporter™ lacZ/Galactosidase Quantitation Kit includes the β-galactosidase substrate, 3-carboxyumbelliferyl β-D-galactopyranoside that produces the blue-fluorescent hydrolysis product, 7-hydroxycoumarin-3-carboxlic acid.
The BLOCK-iT™ Pol II miR RNAi Expression Vector Kit combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript. The pcDNA™6.
The RadPrime DNA Labeling System is ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for the detection of DNA and RNA (1). The RadPrime DNA Labeling System: • Produces probes that can detect nucleic acids on northern or Southern blots, plaque lifts, colony...
Biotin-14-dCTP is used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling. Advantages of using biotinylated DNA probes include: • Convenience and safety—probe preparation generates no radioactive waste • Stability—probes remain active...
Biotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP...