Shop All Immunohistochemistry Kits and Reagents

DAPI Solution (1 mg/mL) Thermo Scientific™

Thermo Scientific Pierce DAPI Nuclear Counterstains are high-purity forms of diamidino-2-phenylindole dye for fixed-cell, fluorescent staining of DNA content and nuclei for cellular imaging techniques.

Also available as a room-temperature-stable, ready-to-use solution: NucBlue Fixed Cell Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

DAPI (diamidino-2-phenylindole) is a blue fluorescent probe that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA, where its fluorescence is approximately 20-fold greater than in the nonbound state. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. DAPI is a classic nuclear counterstain for immunofluorescence microscopy, as well as an important component of high-content screening methods requiring cell-based quantitation of DNA content.

Features of DAPI Nuclear Counterstain:

DAPI dye—diamidino-2-phenylindole is a blue fluorescent stain specific for DNA
Convenient—choose powder or easy-to-use DAPI dye solution (1 mg/mL)
Cellular imaging—cell-permeable dye is effective for fixed-cell staining and quantitation of DNA content
Counterstain—ideal for use alongside detection of specific targets with fluorescent antibodies for fluorescence microscopy or high-content screening (HCS)

Properties of DAPI Fluorescent Dye:

Alternative names: DAPI Stain, DAPI Dye, DNA Content Counterstain
Chemical name(s): 4,6'-diamidino-2-phenylindole, dihydrochloride; 4',6-diamidine-2-phenyl indole; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
Molecular formula: C16H15N5·2HCl
Molecular weight: 350.25
Excitation wavelength: 341±3 nm (near 360 nm when bound to dsDNA)
Emission wavelength: 452±3 nm (456 to 460 nm when bound to dsDNA)
Extinction coefficient: >30,600/M cm at 347 nm in methanol
CAS #: 28718-90-3
Purity: > 95% (most lots >98%) by HPLC at 240 nm
Solubility: >1 mg/mL in water; compound is soluble in DMF, water and various non-phosphate aqueous buffers
Reactive groups: None; binds to minor groove of double-stranded DNA

As a counterstain in fluorescence imaging methods, DAPI is compatible with antibodies and other probes labeled with fluorescein and rhodamine dyes, as well as with Thermo Scientific DyLight Fluors. DAPI has greater photostability than Hoechst dyes, although Hoechst 33342 can be use for live cell imaging while use of DAPI is confined to fixed cells. DAPI is offered in powdered solid and aqueous solution forms.

Applications for DAPI Stain:

• Assaying DNA in solution (ref.4)
• Diagnosing mycoplasmal infection of cell cultures (ref.5)
• Measuring nuclear content and sorting chromosomes in flow cytometry (ref.6)
• Assessing apoptosis (ref.7)
• Detecting nuclei and organellar DNA in immunofluorescent and in situ hybridization procedures (ref.2,8)
• Replacing ethidium bromide for staining DNA in agarose gels (ref.5,9)
• Counterstaining nuclei in histochemical methods when red-fluorescent antibodies have been used to detect specific targets (ref.8)
• Reports also indicate that DAPI will bind to polyphosphates and other polyanions (ref.10), dextran sulfate (ref.11) and SDS (ref.12).

Related Products
DAPI (4',6-diamidino-2-phenylindole, dihydrochloride)

Hoechst 33342 Solution (20 mM) Thermo Scientific™

Thermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques.

Features of Hoechst 33342 Fluorescent Stain:

Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells)
Convenient—provided as an easy-to-use Hoechst dye solution (20 mM)
Cellular imaging—cell-permeable dye is effective for fixed-cell and live-cell staining
Counterstain—ideal for use alongside detection of specific targets with fluorescent antibodies for fluorescence microscopy or high-content screening (HCS)

Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain that is excited by ultraviolet light and emits blue fluorescence at 460 to 490 nm. Hoechst 33342 binds preferentially to adenine-thymine (A-T) regions of DNA. This stain binds into the minor groove of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios.

Properties of Hoechst Fluorescent Dye
As a counterstain in fluorescent imaging, Hoechst dye is compatible with antibodies and other probes labeled with fluorescein and rhodamine dyes, as well as with Thermo Scientific DyLight Fluors. The stable 20 mM aqueous stock solution is essentially ready for use.

Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in combination with 5-bromo-2'-deoxyuridine (BrdU) labeling to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content. Hoechst 33342 and propidium iodide are frequently used together for simultaneous flow cytometric and fluorescence imaging analysis of the stages of apoptosis and cell-cycle distribution.

ProQuantum™ Immunoassays Accessory Kit Invitrogen™

The ProQuantum Immunoassay Accessory Kit provides the common consumable plastics to be used with any ProQuantum immunoassay product, conveniently bundled for first-time users who may be uncertain of what is needed or low-volume users who do not wish to buy these plastics in bulk. Sufficient materials are provided to run 10 assays using 96-well plates. The kit does not include the following items: qPCR plates, cold blocks, and pipette tips.

BSEP Vesicular Transport Assay Reagent Set Gibco™

The BSEP Vesicular Transport Assay Reagent Set is a ready-to-use kit containing all reagents needed for vesicular transport assay except for the vesicles themselves. The reagent set can be used for vesicular transport assay using radioisotope-labeled or non-labeled compound. This kit has enough material to perform 100 vesicular transport assays.

Use The BSEP Vesicular Transport Assay Reagent Set to:

• Investigate the transporter interactions of your drug candidates
• Assess potential for transporter-mediated drug-drug interactions
• Obtain high quality results with a large signal to noise ratios

Concept Behind ABC Transporter Vesicles
ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate’s substrate and drug interaction potential. While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles.

Clear and Reliable Results
Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these 'inside-out' vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter.

Kit Compatibility
Our BSEP Vesicular Transport Assay Reagent Set is designed for use with BSEP Vesicles. The 100 vesicular transport assays that can be performed with this kit will require two BSEP Vesicle products

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

IPTG Thermo Scientific™

Thermo Scientific Pierce IPTG is isopropyl-β-D-thiogalactopyranoside for use with beta-galactosidase substrates such as X-Gal to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene.

The reagent X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase that yields a blue precipitate, is soluble in DMF, and can be used to detect vectors, plasmids, or DNA fragments that contain the β-Galactosidase gene.

IPTG (Isopropyl-β-D-thiogalactopyranoside) may be used to maximize the expression of genes in expression vectors. When screening a lambda bacteriophage library with an antibody, the probability of success can be increased by preventing the expression of the fusion protein until the plaques are well established. By placing a nitrocellulose filter containing the inducer IPTG onto the plate and continuing growth at 37°C, the expression of the potential β-galactosidase-cDNA fusion protein can be induced after 3-4 hours of plaque growth. The nitrocellulose filter is then screened with antibodies. IPTG is also used in combination with X-Gal to detect active β-galactosidase and is practical in applications using bacteriophage M13 and Igt11 vectors.

Features of IPTG:

• X-Gal yields an intense blue precipitate to detect β-galactosidase expression
• Can be combined with IPTG to selectively maximize the detection of β-galactosidase

Alexa Fluor™ 568 Tyramide Reagent Invitrogen™

Alexa Fluor™ 568 Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits made available separately here. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images. Alexa Fluor™ 568 Tyramide Reagent (579/604 ex/em) can be detected using a standard Orange/RFP/TRIC filter cube.

Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

LanthaScreen™ TR-FRET VDR Coactivator Assay Kit, goat Thermo Scientific™

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15127:

The LanthaScreen® TR-FRET Vitamin D Receptor (VDR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential VDR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a VDR ligand-binding domain (VDR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format.

The assay
To run the LanthaScreen® TR-FRET VDR Coactivator Assay, VDR-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based upon the biology of the VDRcoactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET VDR Coactivator Assay Kit contains the VDR-LBD (GST) protein, fluorescein-labeled TRAP220/DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

LanthaScreen™ TR-FRET ERR alpha Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15130:

The LanthaScreen® ERR (estrogen related receptor) alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists (sometimes referred to as antagonists) of coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ERR alpha ligand-binding domain (ERR alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.


In the LanthaScreen® ERR alpha TR-FRET Coactivator Assay, ERR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the ERR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).


Contents and Storage:


The LanthaScreen® TR-FRET ERR alpha Coactivator Assay Kit contains ERR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15128:

The LanthaScreen® TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

In the LanthaScreen® TR-FRET LXR alpha Coactivator Assay, LXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET LXR alpha Coactivator Assay Kit contains LXR alpha-LBD (GST) protein, fluorescently labeled TRAP220/DRIP-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution (30X) Invitrogen™

ReadyProbes Mouse-on-Mouse IgG Blocking Solution blocks endogenous binding when mouse IgG primary antibody is used to label mouse tissue. Most mouse tissues contain mouse IgG antibodies. When an anti-mouse IgG secondary antibody is used on mouse tissue, the antibody cannot distinguish between the endogenous mouse IgG antibodies and the mouse primary antibody used for target protein detection. ReadyProbes Mouse on Mouse IgG Blocking Solution reduces the binding of endogenous antibodies, resulting in increased specific detection in immunohistochemistry (IHC) and immunofluorescence (IF) applications. This reagent is compatible with both fluorescent and chromogenic IHC procedures.

ReadyProbes Mouse-on-Mouse IgG Blocking Solution is provided at a 30X concentration and is diluted to a 1X working concentration (2 drops in 2.5 mL of PBS or TBS). This solution is not used in place of a non-specific blocking solution (e.g., diluted serum), but is used after the tissue has already been blocked with a non-specific solution and before the primary antibody step. Simply add the 1X solution to the tissue and incubate for 30-60 minutes at room temperature.

Human Cardiomyocyte Immunocytochemistry Kit Invitrogen™

The Human Cardiomyocyte Immunocytochemistry Kit enables optimal image-based analysis of two key cardiomyocyte markers: NKX2.5 and TNNT2/cTnT. It is the only kit that offers superior imaging for cardiomyocytes in one box, with a complete set of primary and secondary antibodies, a nuclear DNA stain, and all of the pre-made buffers for an optimized staining experiment.

With the Human Cardiomyocyte Immunocytochemistry Kit, you can:
• Confirm expression of key cardiomyocyte markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by eliminating unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents

Confirm Expression of Key Cardiac Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the immunocytochemistry analysis of cardiomyocytes. The antibodies are well suited for the characterization of cardiomyocytes generated from pluripotent stem cells using the Gibco® PSC Cardiomyocyte Differentiation Kit.

Get More Information Per Sample
The antibodies in the kit have been designed to allow for the specific and simultaneous assessment of NKX2.5 and TNNT2 along with nuclear DNA staining to help save time and precious sample.

Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor® dyes, this kit enables the acquisition of beautiful multiplexed images of cardiomyocyte marker expression using commonly available blue, green, and orange/red filter sets.

Eliminate Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard immunocytochemistry protocols, saving time and decreasing the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.

Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

What You Get
Easily scaled to any culture plate, dish, or slide format, the Human Cardiomyocyte Immunocytochemistry Kit comes with reagents sufficient to stain 50 samples using a 200 µL staining volume. Detailed kit contents can be found below.

LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit Thermo Scientific™

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4839:

The LanthaScreen® TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound’s ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, “addition-only " format.

ProcartaPlex™ Human Viral Reagent Kit Invitrogen™

The ProcartaPlex Viral Reagent Kit is designed to be used with the Coronavirus Simplex kits to combine single analytes into a multiplex assay. This provides maximum flexibility to design assay panels as needed. This kit includes all reagents except for the target-specific beads (sold separately) to run the assay.

ProcartaPlex immunoassays are an ideal solution for assessing multiple protein biomarkers in a single sample.

Suitable sample types: serum, plasma
Sample volume: serum, plasma: 25 µL
Reported application: multiplex immunoassay

LanthaScreen™ TR-FRET PPAR alpha Coactivator Assay Kit, rabbit Thermo Scientific™

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4684:

The LanthaScreen® TR-FRET PPAR (peroxisome proliferator activated receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PPAR alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR alpha ligand-binding domain (PPAR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

Agonist mode:
When running the LanthaScreen® TR-FRET PPAR alpha Coactivator Assay in agonist mode (to identify agonist compounds), PPAR alpha- LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).

Antagonist mode:
When the LanthaScreen® TR-FRET PPAR alpha Coactivator Assay is run in antagonist mode (to identify antagonist compounds), PPAR alpha-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluorescein-coactivator peptide, and Tb-labeled anti-GST antibody (Figure 2). The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode.

Contents and Storage:
The LanthaScreen® TR-FRET PPAR alpha Coactivator Assay Kit contains PPAR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or 4°C).

LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15142:

The LanthaScreen® TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound’s ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, “addition-only " format.
Results per page
    spinner