Shop All Immunohistochemistry Kits and Reagents

ReadyProbes™ Streptavidin/Biotin Blocking Solution (1X) Invitrogen™

ReadyProbes Streptavidin/Biotin Blocking Solution blocks endogenous biotin, biotin receptors, and streptavidin binding sites present in many types of tissue, reducing background signal in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) procedures. This two-component solution (a streptavidin-blocking solution and a biotin-blocking solution) is provided in a ready-to-use format—just add a few drops of the streptavidin component and incubate for 15 minutes, then wash and repeat with the biotin component. ReadyProbes Streptavidin/Biotin Blocking Solution is used after the blocking of cells/tissue with standard non-specific blocking solution (e.g., diluted serum), but before the primary antibody step.

ReadyProbes™ Endogenous HRP and AP Blocking Solution (1X) Invitrogen™

ReadyProbes Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution inhibits endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase activity in formalin-fixed paraffin-embedded tissue sections, frozen tissue sections, and cell preparations. It is provided in a ready-to-use format—just add a few drops and incubate for 10–15 minutes. Endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase activities become problematic when alkaline phosphatase (AP)- or horseradish peroxidase (HRP)- based detection systems are used. ReadyProbes Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution inhibits these endogenous activities, for reduced non-specific signal in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) procedures. This blocker is used before any standard, non-specific blocking solutions (e.g., diluted serum).

ReadyProbes™ Tissue Autofluorescence Quenching Kit Invitrogen™

The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. If the target signal is low enough, then this background signal can interfere with the interpretation of immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence(IF), and in situ hybridization (ISH) assays. The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes most kinds of autofluorescence, with the exception of autofluorescence from lipofuschin granules.

The ReadyProbes Tissue Autofluorescence Quenching Kit contains three components that are combined just before use. Combine components A, B, and C in a 1:1:1 ratio. Mix components A and B together before adding component C. The resulting solution is then applied to the tissue for 2–5 minutes at room temperature. This is done after the blocking, primary antibody, and secondary antibody steps, but before application of any counterstains like nuclear stains.

Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit Thermo Scientific™

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Products
ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit

Endogenous Biotin-Blocking Kit Invitrogen™

The Endogenous Biotin-Blocking Kit provides a method and reagents for a pretreatment that reduces or eliminates background signals when biotin-avidin or biotin-streptavidin detection systems are used to identify cellular targets.

The Endogenous Biotin-Blocking Kit offers:
• Improved results—increased signal-to-noise ratio facilitates cell target identification
• Ease of use—pipette-free dropper bottle offers speed and convenience
• Fast—endogenous background reduction in under an hour
• Flexibility—the kit can be used to help improve results from conventional immunohistochemistry, immunocytochemistry, RNA FISH, and ELISA procedures

Biotin occurs naturally, functioning as an enzyme cofactor in the cytosol and mitochondria of a wide variety of cell types. The Endogenous Biotin-Blocking Kit minimizes interference from the endogenous biotin found in enzymes of mammalian cells and tissues, through a two-step blocking strategy. First, an excess of unlabeled streptavidin is added to the specimen to bind endogenous biotin-rich enzymes. The streptavidin is subsequently blocked with an excess of unlabeled biotin, effectively rendering the cell or tissue sample free of available biotin-binding sites.

Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) Invitrogen™

The Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) enables optimal image-based analysis of two key markers of human pluripotent stem cells (PSCs): OCT4 and SSEA4. This high performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment. To characterize PSCs with other marker combinations, we recommend the use of the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit or the Pluripotent Stem Cell Immunocytochemistry Kit (SOX2, TRA-1-60).

With the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4), you can:
• Confirm expression of key pluripotent stem cell markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents

Confirm Expression of Key PSC Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 is a critical nuclear marker, while SSEA4 is an important cell surface marker.

Get More Information Per Sample
The antibodies in the kit have been designed to allow for the specific and simultaneous assessment of OCT4 and SSEA4 along with nuclear DNA staining to help save time and precious sample.

Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor® dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.

Minimize Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.

Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

What You Get
Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)

LanthaScreen™ TR-FRET ERR alpha Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15130:

The LanthaScreen® ERR (estrogen related receptor) alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists (sometimes referred to as antagonists) of coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ERR alpha ligand-binding domain (ERR alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.


In the LanthaScreen® ERR alpha TR-FRET Coactivator Assay, ERR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the ERR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).


Contents and Storage:


The LanthaScreen® TR-FRET ERR alpha Coactivator Assay Kit contains ERR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

LanthaScreen™ TR-FRET PPAR alpha Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15133:

The LanthaScreen® TR-FRET PPAR (peroxisome proliferator activated receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PPAR alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR alpha ligand-binding domain (PPAR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

Agonist mode:
When running the LanthaScreen® TR-FRET PPAR alpha Coactivator Assay in agonist mode (to identify agonist compounds), PPAR alpha- LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).

Antagonist mode:
When the LanthaScreen® TR-FRET PPAR alpha Coactivator Assay is run in antagonist mode (to identify antagonist compounds), PPAR alpha-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluorescein-coactivator peptide, and Tb-labeled anti-GST antibody (Figure 2). The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode.

Contents and Storage:
The LanthaScreen® TR-FRET PPAR alpha Coactivator Assay Kit contains PPAR alpha-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or 4°C).

Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit Invitrogen™

The Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit enables optimal image-based analysis of four key markers of human pluripotent stem cells (PSCs): OCT4, SOX2, SSEA4, and TRA-1-60. This high performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment. To characterize pluripotent stem cells using only two markers, we recommend us of the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) or the Pluripotent Stem Cell Immunocytochemistry Kit (SOX2, TRA-1-60).

With the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit, you can:
• Confirm expression of key pluripotent stem cell markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents

Confirm Expression of Key PSC Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 and SOX2 are critical nuclear markers, while SSEA4 and TRA-1-60 are important cell surface markers.

Get More Information Per Sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OCT4 & SSEA4 and SOX2 & TRA-1-60) along with nuclear DNA staining to help save time and precious sample.

Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor® dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.

Minimize Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.

Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

What You Get
Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)

eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 570 Invitrogen™

This BrdU Kit for immunohistochemistry (IHC)/immunocytochemistry (ICC) with immunofluorescence detection contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using a streptavidin-conjugated fluorophore. This kit has been optimized for IHC with both frozen and formalin-fixed paraffin embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Conjugate
eFluo 570

Emit
570 nm

Excite
555 nm

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunohistochemical Staining of Frozen Tissue Sections, Immunocytochemistry, Microscopy

LanthaScreen™ TR-FRET FXR Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15140:

The LanthaScreen® TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Pierce™ 16% Formaldehyde (w/v), Methanol-free Thermo Scientific™

Thermo Scientific Pierce Formaldehyde Ampules are vials of high-quality, methanol-free formaldehyde for use as a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid tissue samples.

The availability of a high-quality, ampule-sealed solution of formaldehyde saves valuable time and eliminates the need and hazards associated with preparing the solution directly from paraformaldehyde. Pierce Formaldehyde is carefully prepared directly from granular paraformaldehyde and sealed under an inert atmosphere in 1 mL and 10 mL ampules. Ampule packaging stabilizes the preparation by protecting the solution from both air oxidation and light, allowing access to 'fresh' formaldehyde every time.

Features of Methanol-free 16% Formaldehyde Ampules:

Fresh—formaldehyde solution remains stable and free of contamination in an amber pre-scored ampule.
Sized Right—package sizes geared for applications ranging from culture-targeted cell fixing to in-cell protein-based applications.
Convenient Starting Concentration—16% w/v formaldehyde solution simplifies dilution to the required working concentration.
Minimizes Waste—1 mL and 10 mL package sizes allow selection of product that will result in less waste and minimize disposal concerns.

Application-targeted ampule packaging
The package sizes of the 16% Formaldehyde Solution (formalin) are targeted to both low and high volume users. The appropriate package size is selected based on the most common applications requiring a fresh, methanol-free formaldehyde solution. The 16% (w/v) solution simplifies the dilution for each respective application and the application-directed packaging minimizes waste.

• 1-mL ampules are intended for protein-based in cell applications such as Chromatin Immunoprecipitation (ChIP) or protein interaction discovery.
• 10-mL ampules are intended for bioimaging (high content screening/analysis) such as fixing cells on a culture plate before image analysis.

'Prepared fresh' Sealed in Ampules
Our formaldehyde solution is prepared as a 16% (w/v) solution of paraformaldehyde (pure granular solid) in distilled/deionized water. Solution stabilization is important because formaldehyde solutions oxidize to formic acid and eventually repolymerize to paraformaldehyde. Most protocols requiring methanol-free formaldehyde suggest that the formaldehyde be 'prepared fresh'. This formaldehyde solution is sealed in ampules immediately after formulation under an inert atmosphere. Ampule packaging stabilizes the preparation allowing access to 'fresh' formaldehyde each time.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit Thermo Scientific™

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody. The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity. Ultra-Sensitive Kits are offered as a Standard Kit and as Complete Kits, which contain biotinylated secondary antibodies and appropriate block serum for use with mouse or rabbit antibodies, respectively.

Related Products
ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Standard Staining Kit
Ultra-Sensitive ABC Peroxidase Mouse IgG Staining Kit

PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green

The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands. The kit uses insect cell-expressed, human PR ligand binding domain tagged with glutathione-S-transferase (GST) (also available separately) and a novel, high-affinity, fluorescent progesterone receptor ligand (Fluormone™ PL Green) in a homogenous, mix-and-read assay format.

This kit produces a green color read-out; a 'red' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact us at drugdiscoverytech@lifetech.com.

The PolarScreen Progesterone Receptor Competitor Assay is:

• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—fluorescence polarization assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs

How the Assay Works
An N-terminal fusion of glutathione transferase to the ligand-binding domain of the human progesterone receptor (PR-LBD(GST)) is added to a fluorescently-tagged progesterone ligand (Fluormone PL Green) in the presence of competitor test compounds in microwell plates. The presence of effective competitors prevents the formation of a PL Green/PR-LBD(GST) complex, resulting in a decrease of the polarization value. The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for PR-LBD(GST).

Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

LanthaScreen™ TR-FRET PPAR gamma Coactivator Assay Kit, rabbit

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4548:

The LanthaScreen® TR-FRET PPAR gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR gamma ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a Peroxisome Proliferator Activated Receptor (PPAR) gamma ligand-binding domain (PPAR gamma-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format.

Agonist mode
To run the LanthaScreen® TR-FRET Peroxisome Proliferator Activated gamma Receptor Coactivator Assay in agonist mode (to identify agonist compounds), PPAR gamma-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After an incubation period at room temperature, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR gamma-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Antagonist mode
When the LanthaScreen® TR-FRET Peroxisome Proliferator Activated gamma Receptor Coactivator Assay is run in antagonist mode (to identify antagonist compounds), PPAR gamma-LBD is added to ligand test compounds followed by addition of a mixture of agonist, fluores-cein-coactivator peptide, and Tb-anti-GST antibody. The concentration of agonist used in this mode is the EC80 concentration as determined by first running the assay in agonist mode (Figure 2).

Contents and Storage:
The LanthaScreen® TR-FRET PPARγ Coactivator Assay Kit contains PPAR gamma-LBD (GST) protein, fluorescently labeled TRAP220/ DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
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