These products include protein mass spectrometry supplies, reagents, and kits to fractionate, purify, isolate, clean up, and recover total or post-translational modification (PTM)-specific classes of processed peptide fragments for proteomics analysis by mass spectrometry (MS).
Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. The polymer-based hydrophobic resin contained in each column provides excellent binding and recovery characteristics for...
Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spectra.
Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein. Removal of albumin, immunoglobulins, and other abundant proteins is essential for the study of low-abundance...
The Thermo Scientific Pierce Intact Protein Standard Mix is a high quality mixture of recombinant proteins for use as a quality control standard for LC and LC/MS, LC/MS method development, and intact protein mass measurements.
The Invitrosol™ LC/MS (Liquid Chromatorgraphy/Mass spectrometry) Protein Solubilizer Kit contains a novel, proprietary surfactant blend for simplified preparation of hydrophobic and hydrophilic proteins or peptides for ESI (Electrospray Ionization) mass spectrometry (MS) experiments.
Pierce colorimetric or fluorescent quantitative peptide assays are easy mix-and-read microplate assays with stable signals for accurate and robust measurement of peptide digest samples. A high-quality peptide digest reference standard is provided as a stand-alone, or in the kits for use in the...
Protein characterization, identification, and quantification by MS begins with efficient, reproducible protein digestion. Sequence specific proteases are utilized to cleave proteins into smaller fragments or peptides. Trypsin is the protease of choice for protein digestion.
The Thermo Scientific Pierce LTQ Velos ESI Positive Ion Calibration Solution is a mixture of highly purified ionizable molecules designed for positive mode calibration of Thermo Scientific LTQ Velos series mass spectrometer instruments.
In order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by...
The Thermo Scientific AccelerOme Label-Free MS Sample Prep Kit provides ready-to-go reagents for efficient and robust proteomics mass spectrometry (MS) sample preparation for use with the AccelerOme Automated Sample Prep System.
The Thermo Scientific Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.
The Thermo Scientific Pierce FlexMix Calibration Solution is a mixture of 16 highly pure, ionizable components (mass ranges: 50 to 3000 m/z) designed for both positive and negative ionization calibration using Orbitrap Tribrid Series 3.
The Thermo Scientific™ Pierce™ BSA Protein Digest Standard, LC-MS Grade, is a validated protein digest optimized for use as a quality control sample for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples.
The Pierce Quantitative Fluorescent Peptide Assay reagents include peptide assay buffer, fluorescent peptide labeling reagent, and a peptide digest assay standard for the quantitative measurement of peptide concentrations.
Immunoprecipitation is often necessary for the detection and quantitation of low-abundance proteins or rare signaling events. Mass spectrometry analysis relies on two methods for the detection of low-abundance proteins: single reaction monitoring (SRM) and parallel reaction monitoring (PRM), which...